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Citations for New England Biolabs :
801 - 850 of 1706 citations for Urothelial Cell Culture Supplements since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... Each electroporation cuvette (BTX) contained 25 μL of 10beta electrocompetent cells (NEB) and 5 μL of cleaned master tube DNA from the previous golden gate barcoding step ...
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bioRxiv - Biochemistry 2022Quote: ... competent cells and were purified using NEB Monarch Miniprep Kits (NEB, Canada) as per manufacturer’s protocol.
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bioRxiv - Cancer Biology 2019Quote: ... and the cell pellets were resuspended in 345μl of 1× NEBuffer2 (NEB) per 5 million cell aliquot ...
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bioRxiv - Genetics 2019Quote: ... Clones were transformed into electrocompetent 10-Beta cells (New England Biolabs # C3020) using a 96-well plate electroporator using fresh electroporation plates (BTX 45-0450-M) ...
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bioRxiv - Genomics 2019Quote: ... Nuclei were extracted by incubation of cells in Nuclear Extraction Buffer (NEB) containing 10 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2020Quote: ... The ligation mix was transformed using NEB stable competent cells (NEB, C3040) for single colony isolation ...
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bioRxiv - Genomics 2019Quote: ... The 3XFlag-pA-Tn5-Fl plasmid was transformed into C3013 cells (NEB) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... 5 μL of this reaction mixture were transformed into XL10Gold cells (NEB).
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bioRxiv - Genetics 2020Quote: ... and stable competent cells were transformed with the resulting construct(NEB, C3040). Ligation was confirmed by PCR and Sanger sequencing ...
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bioRxiv - Biochemistry 2021Quote: ... coli BL21 T7 Express lysY/Iq cells (New England BioLabs, cat. # C3013). The bacterial cultures were grown at 37 °C to an optical density (OD600 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with 0.1 μM TMR-Star (S9105S, New England BioLabs) for at least 30 min ...
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bioRxiv - Synthetic Biology 2021Quote: ... The cells were resuspended in 1 mL of 1× NEBuffer 2.1 (NEB) and homogenized by grinding to a fine powder in liquid nitrogen ...
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bioRxiv - Microbiology 2021Quote: ... cell lysates were treated with endoglycosidase H (Endo H) (New England BioLabs) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were labeled with 500nM SNAP-Surface 649 (New England Biolabs, #S9159S) for 5 minutes at 37°C for TIR-FM imaging or 1μM permeable SNAP-Cell 647-SiR (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were supplemented with 1X Protein MetalloPhosphatase buffer (New England Biolabs) and 1 mM MnCl2 ...
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bioRxiv - Cancer Biology 2022Quote: ... adjacent normal and cell lines (Qiagen RNeasy or NEB Monarch Total RNA). Libraries were prepared using Illumina TruSeq ...
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bioRxiv - Biochemistry 2022Quote: DNMT3A PWWP domain was expressed in BL21(DE3) cells (New England Biolabs) using the same protocol as above ...
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bioRxiv - Biochemistry 2022Quote: ... EmGtsf1 expression vector was transformed into BL21(DE3) cells (New England Biolabs). Transformed cells were grown in LB supplemented with 1 μM ZnSO4 at 37°C until OD600 ∼0.6–0.8 ...
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bioRxiv - Biochemistry 2022Quote: ... Each protein was expressed in T7 Express cells (New England BioLabs, MA) grown in Terrific Broth at 37°C following induction with 0.5mM IPTG ...
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bioRxiv - Cell Biology 2022Quote: ... 1.5 μl of 0.6 μM SNAP-Cell 647-SiR (New England Biolabs) was injected into the mouse vitreous under direct visualization using a Zeiss operating microscope ...
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bioRxiv - Cell Biology 2022Quote: ... cells were labelled in suspension with SNAP-Surface 549 (New England Biolabs) for 30 minutes at 37°C and FACS-sorted to select for SNAP-GLP-1R-expressing ones ...
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bioRxiv - Microbiology 2021Quote: ... Ligations were used to transform NEB 5-alpha competent cells (NEB C2987H) and the cloned spacer was verified by Sanger sequencing using primer PSP108 ...
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bioRxiv - Biophysics 2021Quote: ... the library was transformed into elec-trocompetent NEB 10-beta cells (NEB) to yield ¿1000x transformants per gene ...
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bioRxiv - Genomics 2022Quote: ... The cells were treated with 100 U of MboI (New England Biolabs) for chromatin digestion ...
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bioRxiv - Biochemistry 2022Quote: ... cells were subjected to RNase H (15U/well) or RNase H (NEB) buffer only treatment for 4 hr and RNase III or RNase III buffer (Ambion ...
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bioRxiv - Biochemistry 2022Quote: ... Ea1174 and AncCDT-5(WAG) were expressed in BL21(DE3) cells (NEB): transformed cells were grown in LB media supplemented with 100 mg/L ampicillin to OD600 ∼0.7 at 37°C ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli cells were used (New England Biolabs GmbH, Germany,Art. No. C2987). This strain was grown in Luria-Bertani (LB ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 μL of ligation products were then transformed into competent cells (NEB). Transformation and plasmid DNA recovery were performed as previously described ...
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bioRxiv - Cell Biology 2019Quote: ... SNAP-Cell® 647-SiR (SiR-SNAP 1:2000) (New England Biolabs) was used to detect SNAP-tagged alleles ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli strain DH10-β cells (45 μL each; C3019I, New England Biolabs) thawed on ice for 10 minutes and mixed gently by tapping ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were grown in DH10-β outgrowth medium (New England Biolabs, B9035S) for transformation ...
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bioRxiv - Microbiology 2021Quote: ... Assemblies were transformed into DH5-alpha chemically competent cells (New England Biolabs) and correct clones were identified by restriction profile and Sanger sequencing (Genewiz ...
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bioRxiv - Synthetic Biology 2020Quote: ... Separately seeded cells were transfected with pCMV-GLuc2 plasmids (1 μg, NEB) and GLuc activity was measured using the same kit ...
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bioRxiv - Genomics 2019Quote: ... The library was then transformed into 10-beta electrocompetent cells (NEB C3020), grown in 100 mL of LB+Amp ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli cells were incubated at 37°C (30°C for NEB stable), 200 min-1 either in 5 ml (for a high copy ...
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bioRxiv - Biochemistry 2019Quote: ... The cleared cell lysate was applied to Amylose resin (New England Biolabs) followed by washing with buffer A and elution with buffer A containing 10 mM maltose ...
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bioRxiv - Cell Biology 2020Quote: ... cells were quenched with a non-fluorescent bromothenylpteridine (BTP; New England Biolabs) at 2 µM final concentration ...
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bioRxiv - Microbiology 2021Quote: ... Ligation mixtures were transformed into chemically competent DH5α cells (New England Biolabs) and verified by miniprep and restriction digests ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GFP-fusion proteins were purified from BL21(DE3) cells (NEB, # C2527H) using an N-terminal H6-tag ...
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bioRxiv - Microbiology 2020Quote: ... coli Top10 cells and purified by using a Monarch miniprep kit (NEB). A Ty tag DNA sequence was inserted at the 3’-end of the gat1 coding sequence using a Q5 site directed mutagenesis kit (NEB ...
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bioRxiv - Genomics 2020Quote: ... concentrated 4x and transformed in electrocompetent E.coli cells (10-beta Electrocompetent, NEB). Cells were recovered in SOC medium and plated on LB with Ampicillin ...
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bioRxiv - Microbiology 2020Quote: ... DNA was transformed into NEB 5-alpha high efficiency competent cells (NEB). Insert size was verified with PCR and purified plasmids were sequenced using Sanger sequencing.
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bioRxiv - Genomics 2021Quote: ... cells were lysed and digested with the MboI restriction enzyme (NEB, R0147). Biotin-14-dATP was used to mark the DNA ends ...
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bioRxiv - Immunology 2022Quote: ... a 1 in 200 dilution of SNAP-Oregon cell permeable ligand (NEB) was added.
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bioRxiv - Cancer Biology 2022Quote: ... then transformed into NEB-a Competent Cells (New England Biolabs, Ipswich, MA). A full listing of the primer sequences used to generate all the plasmids used in this study is provided in the Supplemental file ...
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bioRxiv - Biophysics 2022Quote: ... TnsC clones were transformed into Escherichia coli BL21(DE3) competent cells (NEB), while TnsB and S15 clones were transformed into homemade T1 phage-resistant pRIPL BL21(DE3) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with 1 mM vanadate (New England BioLabs, Ipswich, MA.).
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bioRxiv - Genomics 2022Quote: ... Purified plasmids were electroporated to ElectroMAX Stbl4 Competent Cells (New England Biolabs) and amplified.
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bioRxiv - Cell Biology 2022Quote: ... the permeabilized cells were treated with 25 units of MboI (R0147, NEB) at 37°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... according to manufacturer’s recommendations and transformed into NEB5α cells (New England Biolabs). Transformants were selected on LB-Cm plates ...