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Citations for New England Biolabs :
1101 - 1150 of 3671 citations for Human TGF beta 3 TGFB3 qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Pu TKamplicon (5’-CTGTTTTCATTCTGCCTTTTGACCATAGAGCCCACCGCATCC-3’ and 5’-GCCAACAAAGAAAGCCTCACTACC GGGTAGGGGAGGCG -3) and Gibson Assembly master mix (NEB) following the manufacturer’s guidelines.
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bioRxiv - Molecular Biology 2021Quote: ... 3 ml of Thermolabile ExoI (BioLabs) was added to each reaction and samples were incubated for 15 min at 37 °C ...
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bioRxiv - Bioengineering 2021Quote: 3 μL of 5′-deadenylase (NEB) were added to ligation reaction,
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bioRxiv - Molecular Biology 2021Quote: ... Then 3 μl USER Enzyme (NEB) was used with size-selected ...
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bioRxiv - Neuroscience 2020Quote: ... 3’ overhangs removed with Klenow (NEB) to form blunt ends ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 µl T4 DNA ligases (NEB), and 2 µl of a 15 µM Illumina indexed adapter at room temperature for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL 10xDNase I Buffer (NEB), and 24 μL WB2 ...
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bioRxiv - Plant Biology 2022Quote: ... and 3 (NEB, catalog No. E7500S) following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... 3 μL USER Enzyme (NEB, USA) was used with size-selected ...
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bioRxiv - Microbiology 2024Quote: ... and 3 µL RNase A (NEB) in volumes normalized to OD600 of culture samples ...
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bioRxiv - Genomics 2023Quote: ... 3 µL 10x NEBuffer 4 (NEB), 3 µL of 1 mM each dATP/ddCTP/ddGTP/ddTTP (dATP/ddBTP ...
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bioRxiv - Molecular Biology 2023Quote: ... and 3 μl QuickCIP enzyme (NEB) at 37°C for 10 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... 3 µl USER Enzyme (NEB, USA) was used with size-selected ...
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bioRxiv - Genomics 2023Quote: ... Klenow Fragment (3’→5’ exo-) (NEB) was diluted in 1X NEBuffer 2 to a final concentration of 2 U/μL ...
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bioRxiv - Neuroscience 2023Quote: ... Then 3 μL USER Enzyme (NEB) was used with size-selected ...
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bioRxiv - Microbiology 2023Quote: ... 3 µL 10x reaction buffer (NEB), 0.25 µl 1 M MgCl2 and 0.3 µl Rnasin ribonuclease inhibitor (Promega ...
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bioRxiv - Microbiology 2024Quote: ... and 3 μL of QuickCIP (NEB). The reaction was incubated at 27°C for 20 minutes followed by inactivation at 80°C for 2 minutes ...
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bioRxiv - Systems Biology 2024Quote: ... 3 µL BsiWI-HF (NEB, R3553), 3 µL MluI-HF (NEB ...
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bioRxiv - Systems Biology 2024Quote: ... 3 µL MluI-HF (NEB, R3198), and nuclease-free water to 150 µL at 37 °C for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 μl of XbaI (NEB R0145S) was used to digest 6 μg of the pcDNA3.1-Cas9 vector ...
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bioRxiv - Genomics 2024Quote: ... 3 µL 10x ThermoPol buffer (NEB), 3 µL H2O and Therminator IX (NEB 10 U/µL ...
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bioRxiv - Genomics 2024Quote: ... 3 µl of USER (NEB # E7103L) was added for 20 min at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... we designed primers for cloning into the pLEX-MCS vector using Gibson Assembly (NEB). To amplify ETV7 ...
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bioRxiv - Molecular Biology 2021Quote: ... and NEBNext Multiplex Oligos for Illumina Primer sets 1 and 2 (New England Biolabs). The libraries were amplified with 7 PCR cycles using Herculase II Fusion Polymerase kit (Agilent) ...
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bioRxiv - Molecular Biology 2021Quote: ... indexed using NEBnext Multiplex Oligos for Illumina Dual Index Primers (New England Biolabs E7600S) and sequenced simultaneously using a Illumina HiSeq4000 System.
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bioRxiv - Genomics 2021Quote: ... Libraries were PCR amplified with dual index barcode primers for Illumina sequencing (NEB E7600) using Q5 DNA polymerase (NEB M0491 ...
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bioRxiv - Bioengineering 2021Quote: ... and amplified by PCR with barcoded universal primers NEBNext Multiplex Oligos for Illumina (NEB), using Kapa HiFi Polymerase (KAPA Biosystems) ...
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bioRxiv - Biophysics 2021Quote: ... Primer extension was initiated by the addition of AMV reverse transcriptase (New England Biolabs) and allowed to proceed for 10 min ...
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bioRxiv - Biochemistry 2021Quote: ... Extra RT primers were removed by Exonuclease I (100 U; M0293S, NEB, Ipswich, MA) for 2 hr at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... the free primer was removed by Exonuclease I (80 U; M0293S, NEB, Ipswich, MA), and a 3’ DNA adaptor was ligated onto the cDNA product with T4 RNA Ligase 1 ...
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bioRxiv - Microbiology 2020Quote: ... Annealed primers were then diluted to 1 µl in 200 µl and ligated (NEB) to the digestedand dephosphorylated Cas9 vector.
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bioRxiv - Neuroscience 2020Quote: ... generated template DNA by primer annealing with the NEBNext High-Fidelity polymerase (NEB, M0541S), and carried out in vitro transcription using the HiScribe T7 Kit (NEB ...
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: The primers were annealed and ligated with psiCHECK2 digested with XhoI and NotI (NEB). The pCDH vectors harboring single EBV miRNAs were used as described (Tagawa et al. ...
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bioRxiv - Genomics 2022Quote: ... NEBNext Multiplex Oligos for Illumina® Dual Index Primers (cat. #E7600, New England Biolabs), and AMPure XP Beads (cat ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was generated with oligo dT18 primer using the Protoscript II reverse transcriptase (NEB) in the presence of Murine RNase inhibitor (NEB) ...
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bioRxiv - Microbiology 2021Quote: ... was used to generate single-strand cDNA using random primer mix (NEB, Hitchin, UK). ARTIC V3 PCR amplicons from the single-strand cDNA were generated following the Nanopore Protocol of PCR tiling of SARS-CoV-2 virus (Version ...
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bioRxiv - Neuroscience 2021Quote: ... Mutagenesis primers were designed using the “substitution” feature in NEBaseChanger v1.2.9 (New England Biolabs). Mutagenesis occurs at nucleotide 805-807 (GGA→ GAA ...
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bioRxiv - Immunology 2022Quote: ... The oligonucleotide was used with primer R1(RB202)* in a NEBuilder assembly reaction (NEB). The resulting plasmid was transformed in electro-competent E ...
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bioRxiv - Plant Biology 2020Quote: ... 1μL of each primer and 2U Phusion® High-Fidelity DNA Polymerase (NEB, USA) for 35 cycles (98 °C for 30s ...
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bioRxiv - Synthetic Biology 2020Quote: ... 6 μl primers (10 μM) and 50 μl Q5 high fidelity master mix (NEB). The thermocycling parameters were ...
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bioRxiv - Synthetic Biology 2021Quote: ... Primers were ordered from IDT with Q5 Tms (calculated via the NEB Tm calculator) of 70-72 degrees ...
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bioRxiv - Immunology 2021Quote: ... and amplified by PCR with barcoded universal primers NEBNext Multiplex Oligos for Illumina (NEB), using Kapa HiFi Polymerase (KAPA Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... was used to generate single-strand cDNA using random primer mix (NEB, Hitchin, UK). ARTIC V3 PCR amplicons from the single-strand cDNA were generated following the Nanopore Protocol of PCR tiling of SARS-CoV-2 virus (Version ...
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bioRxiv - Cell Biology 2021Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) (NEB E7335) according to the manufacturer’s protocols ...
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bioRxiv - Genetics 2020Quote: ... amplified using primers with KpnI and BamHI RE sites with Phusion Master Mix (NEB), digested ...
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bioRxiv - Genomics 2021Quote: ... cDNA was prepared from viral RNA using random primer mix (NEB, Massachusetts, United States), and LunaScript® RT SuperMix Kit (Cat# E3010G) ...
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bioRxiv - Developmental Biology 2022Quote: ... with SP6 and T3 primers and Phusion High Fidelity DNA polymerase (New England, BioLabs). The purified product was used as a template for the transcription reaction using the mMessage mMachine SP6 RNA transcription kit (AM1340 ...
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bioRxiv - Molecular Biology 2022Quote: ... rRNA was reverse transcribed using a linker-specific primer and Protoscript II RT (NEB) to generate the first strand of cDNA ...
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bioRxiv - Genomics 2024Quote: ... and amplified by PCR with barcoded universal primers NEBNext Multiplex Oligos for Illumina (NEB), using Kapa HiFi Polymerase (KAPA Biosystems) ...
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bioRxiv - Biochemistry 2023Quote: ... Primers for generating mutations for MsbA were designed using the online tool NEBaseChanger (NEB) and carried out using the KLD enzyme mix (NEB ...