Labshake search
Citations for New England Biolabs :
9901 - 9950 of 10000+ citations for Mouse Tyrosinase Related Protein 1 TYRP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... ChIP-seq libraries were generated using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB # E7645S) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 cycles on a Covaris E220 focused ultrasonicator and were subsequently processed using NEBNext Enzymatic Methyl-Seq kit (NEB #7120) following the manufacturer protocol ...
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bioRxiv - Biochemistry 2024Quote: ... a codon-optimized synthetic gene product was synthesized in two overlapping halves by Twist Biosciences and integrated into a vector using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB). pNH-TrxT was a gift from Opher Gileadi (Addgene plasmid # 26106 ...
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bioRxiv - Cell Biology 2024Quote: ... The sgRNAs were synthesized by in vitro transcription from DNA oligonucleotide templates using the HiScribe T7 Transcription Kit (New England Biolabs). The transcribed products were subsequently purified using the RNA Clean & Concentrator (ZYMO RESEARCH) ...
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bioRxiv - Cancer Biology 2024Quote: ... Guide RNAs were generated in house using Highscribe™ T7 Quick High Yield RNA synthesis kit (New England Biolabs Inc.), Alt-R™ S.p ...
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bioRxiv - Plant Biology 2024Quote: ... graminearum or mock inoculated was treated with DNAse and cDNA was synthesized using LunaSCript RT MasterMix kit (New England Biolabs), with random hexamer and oligo-dT primers ...
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bioRxiv - Neuroscience 2024Quote: ... The enriched mRNA was fragmented into short fragments using fragmentation buffer and reverse transcribed into cDNA using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530 ...
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bioRxiv - Physiology 2024Quote: ... DNA libraries were generated as per the manufacturer’s protocol using the NEBNext® Poly(A) mRNA Magnetic Isolation Module kit (New England Biolabs). 1μg total RNA per sample was inputted and adaptor ligation and PCR enrichment was performed using the NEBNext® Multiplex Oligos for Illumina® kit as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... IVT was performed following the manufacturer’s protocol for the HiScribe T7 High Yield RNA Synthesis Kit (NEB, Ipswich, MA, USA). Polyadenylated RNA was added to the IVT RNA control at a ratio of 1:1000.
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bioRxiv - Neuroscience 2024Quote: ... The four separate fragments were amplified and inserted into a vector containing the Tol2 sites35 using the Gibson Assembly Cloning Kit (New England Biolabs).
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bioRxiv - Molecular Biology 2024Quote: ... The 3′ adapter was conjugated with an amino CA linker instead of dCC at the 3′ end (GeneDesign) and then adenylated at the 5′ end with a 5′ DNA adenylation kit (NEB). Four random nucleotides were added in the 3′ and 5′ adapters [(5′ -rAppNNNNTGGAATTCTCGGGTGCCAAGG/amino CA linker-3′ ...
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bioRxiv - Biochemistry 2024Quote: ... 1-5 mL of cell pellet was harvested for plasmid extraction with the Monarch Plasmid DNA Miniprep Kit (New England Biolabs) (T1010) ...
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bioRxiv - Biochemistry 2024Quote: ... with C-terminal TEV, GFP, 7xHis, and StrepII (Thawani et al., 2020) using the Q5 Site Directed Mutagenesis Kit (NEB). All three constructs were expressed using the Bac-to-Bac Sf9 cell expression system (Invitrogen) ...
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bioRxiv - Genomics 2024Quote: ... TruSeq-barcoded RNAseq libraries were generated with the NEBNext Ultra II Non-Directional RNA Library Prep Kit (New England Biolabs). Each library was quantified with a Qubit (dsDNA HS kit ...
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bioRxiv - Developmental Biology 2024Quote: ... Construction of pUAST- attB-Cic(1-15)A-SBP and pUAST-attB-Cic20A-mVenus was carried out by assembling gene fragments using the HiFi DNA assembly Kit (NEB). Construction of the other subgroups of Cic mutants was done by assembling different parts of Cic generated by overlap PCR using wild type Cic ...
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bioRxiv - Neuroscience 2024Quote: ... were cloned N-terminally to human CISD1 and point mutations introduced by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis kit (NEB). All plasmids were sequence verified ...
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bioRxiv - Biochemistry 2024Quote: ... was introduced into linearized pEGFP-C1 ΔSV40-Stop with Xho I and BamH I use the HiFi DNA Assembly Cloning Kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... 5 ng of ChIP and Input DNA was used to generate ChIP-seq libraries using the NEBNext Ultra DNA library prep kit for Illumina (New England Biolabs, NEB).
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bioRxiv - Genomics 2024Quote: ... Following end repair with NEBNext® End Prep Ultra II Kit (Supplemental Table 9, Cat No. E7645, NEB, Ipswich, MA), custom iTru adapters were ligated to amplicons with NEBNext® Ultra II Ligation Master Mix (Cat No ...
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bioRxiv - Biochemistry 2024Quote: ... Of the rRNA-depleted samples directional libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) in accordance to the recommended protocol ...
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bioRxiv - Biophysics 2024Quote: ... Plasmid DNA was extracted using the Qiagen Plasmid Plus Midi kit and the library inserts were obtained through double endonuclease digestion (HindIII-HF and NheI-HF, NEB) followed by agarose gel purification achieved by a combined use of centrifugal filter units (Millipore ...
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bioRxiv - Biochemistry 2024Quote: ... Lec5 and Lec9 CHO cells was extracted using the Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB, T3050L). Resulting gDNA was then sheared by passing each sample 25 times through a 26G needle ...
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bioRxiv - Bioengineering 2024Quote: ... PolyA selection and stranded RNA-seq library preparation were performed with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760; New England Biolabs). Then ...
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bioRxiv - Genomics 2024Quote: ... 1µg RNA was used for first strand cDNA preparation by using ProtoScript II Reverse Transcriptase kit followed by Phusion High-Fidelity DNA Polymerase (NEB). RT-qPCR was performed with LunaScript RT Supermix (NEB ...
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bioRxiv - Zoology 2024Quote: ... The transcriptome sequencing libraries were prepared with poly A selection using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) and were sequenced using the NovaSeq 6000 instrument (Illumina ...
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bioRxiv - Immunology 2024Quote: ... 100 ng of the WTA library was subjected to fragmentation/end-repair/A-tailing using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs). Purified adapter-ligated products were subjected to index PCR ...
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bioRxiv - Genetics 2024Quote: ... after extracting total RNA from the testis (three replicates per genotypes) using the Monarch total RNA mini prep Kit (New England Biolabs), we generated RNA-seq libraries using the NEBNext Rrna Depletion Kit (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... 1μg RNA was used for sequencing libraries using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to each sample ...
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bioRxiv - Microbiology 2024Quote: High molecular weight DNA for long-read sequencing was extracted using the Monarch HMW DNA extraction kit for tissue (NEB), with the specific protocol for Gram-negative bacteria ...
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bioRxiv - Cancer Biology 2024Quote: ... The purified CASP3 gene fragment was ligated into the linearized pEGFP-N3 vector using the quick ligationTM kit according to the manufacturer’s instructions (NEB, B2200S). The resulting mixture was transformed into competent DH5α bacteria (NEB ...
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bioRxiv - Microbiology 2024Quote: ... Library preparation with indexing was performed by the University of Kansas Genome Sequencing core facility with NEB Next RNA Library kit (NEB). RNAseq was performed using an Illumina NextSeq2000 high-output system with paired-end reads of 50 bp ...
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bioRxiv - Immunology 2024Quote: ... Libraries were prepared from rRNA depleted samples using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) and sequenced on the Novaseq 6000 S4 (Illumina) ...
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bioRxiv - Cell Biology 2024Quote: ... and R211K were introduced into the mKO2-RIβ plasmid using a site-directed mutagenesis kit (New England BioLabs, Ipswich, MA). N-terminal His-tagged human RIβ-WT and RIβ-L50R constructs ...
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bioRxiv - Neuroscience 2024Quote: ... The remaining steps of library preparation were performed using and the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). Adapters and PCR primers were purchased from New England BioLabs ...
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bioRxiv - Molecular Biology 2024Quote: Libraries of immunoprecipitated DNA were generated from 3 ng of starting DNA with the NEBNext Ultra DNA Library Prep kit for Illumina (New England Biolabs) according to manufacturer’s instructions at the CRG Genomics Core Facility (Barcelona ...
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bioRxiv - Synthetic Biology 2024Quote: ... These synthesized fragments were assembled into the Level 0 entry vector pYTK001 using the NEBridge Golden Gate Assembly Kit (BsmBI-v2) (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA from libraries was prepared according to the instructions of the “NEBNext Ultra Directional RNA Library Prep kit for Illumina” (New England Biolabs), following the “Poly(A ...
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bioRxiv - Molecular Biology 2024Quote: 50-100 ng of purified PCR product was prepared for sequencing using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Molecular Biology 2024Quote: 175-225 ng of purified PCR product was prepared for sequencing using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Microbiology 2024Quote: Codon-optimized full-length SARS-CoV-2 S protein gene was cloned into Topo cloning vector and 22 N-glycosylation sites were mutated individually from N to Q using a Q5 Site-Directed Mutagenesis Kit (NEB). The full-length or C19 truncated fragments were amplified and inserted into plvx-puro vector ...
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bioRxiv - Microbiology 2024Quote: ... the construct generated above with the WT rel allele in pIMAY (pRel-swap, Table S2) was used for site directed mutagenesis with the Q5-Site-Directed-Mutagenesis kit (NEB) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2024Quote: ... Isolation of mRNA was achieved via poly(A) enrichment before library preparation (Ultra II Directional RNA library prep kit, New England Biolabs). Libraries were sequenced on an Illumina NovaSeq with 40M 150bp paired end reads per sample.
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bioRxiv - Microbiology 2024Quote: Genomic DNA purification for use in cloning or whole-genome sequencing was purified using the Monarch HMW DNA Extraction Kit for Tissue (T3060L, New England Biolabs) according to manufacturer’s instruction with one modification ...
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bioRxiv - Microbiology 2024Quote: ... and held at 72°C for 15 minutes before purification by PCR and DNA cleanup kit (New England Biolabs, T1030S) according to manufacturer instructions with the following modifications ...
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bioRxiv - Microbiology 2024Quote: ... Illumina sequencing libraries were generated using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2024Quote: ... ΔPYD and HD mutants were generated from full length IFI207-HA plasmids and the R1-ΔPYD mutant from R1-IFI207 using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). IFI207-HIN plasmids were generated by PCR amplification of the HIN domain from full length IFI207-V5 expression plasmids ...
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bioRxiv - Molecular Biology 2024Quote: ... and Rpa2WH (Rpa2178-268) were cloned from the pRSFduet(+) constructs using the Q5 site directed mutagenesis kit (E0554, New England Biolabs).
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bioRxiv - Microbiology 2024Quote: ... mutagenic primers were designed using the New England Biolabs online software NEBaseChanger (primer sequences are available at doi: XXXX. The Q5 Site-Directed Mutagenesis Kit (New England BioLabs) was then used with the A/Baltimore/R0675/2019 hemagglutinin plasmid to produce plasmids with the desired mutations ...
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bioRxiv - Molecular Biology 2024Quote: 200 ng of pooled PCR product was prepared for sequencing using the NEB-Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Molecular Biology 2024Quote: Plasmid pHERD30t with and without overlapping AluI/BREX site was purified from BREX+ (E. coli HS) or BREX- cells (E. coli BW25113) using Monarch Plasmid Purification Kit (NEB). 100 ng of plasmid was restricted with AluI (ThermoScientific ...