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Citations for New England Biolabs :
901 - 950 of 3027 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Phusion® High-Fidelity DNA Polymerase was obtained from NEB (New England Biolabs, M0530S). Glycine was obtained by VWR (1042011000) ...
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bioRxiv - Molecular Biology 2024Quote: Construct generation: all cloning was performed using restriction enzymes from New England Biolabs (NEB) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... using BsaI digestion and T4 DNA ligation (respectively M0202S and M0202S both from NEB). Correct ligation was confirmed by digestion with enzymes XmnI ...
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bioRxiv - Molecular Biology 2024Quote: ... from the Nluc PCR template as described 65 and capped using ARCA (NEB, S1411) as described 47 ...
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bioRxiv - Molecular Biology 2024Quote: ... qRT-PCR from total RNA was performed using Luna Universal One-step kit (NEB) per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The complexes were eluted from the beads by TEV Protease (New England Biolabs/ NEB) cleavage in a buffer containing (10 mm Tris (pH 8) ...
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bioRxiv - Cancer Biology 2024Quote: ... The complexes were eluted from the beads by TEV Protease (New England Biolabs/ NEB) cleavage in a buffer containing (10 mm Tris (pH 8) ...
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bioRxiv - Microbiology 2024Quote: ... and purified from agarose gel using Monarch Gel purification kit (New England Biolabs®). They were ligated using T4 ligase from New England Biolabs® ...
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bioRxiv - Microbiology 2024Quote: We synthesized cDNA from RNA using Luna RT Mix (New England Biolabs, Ipswich, MA) and measured gene expression by qPCR performed with 2X Universal SYBR Green Fast qPCR Mix (ABclonal ...
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bioRxiv - Microbiology 2024Quote: ... gene fragments were amplified from genomic DNA using Phusion DNA Polymerase (New England Biolabs) with complementary plasmid insertion site overhangs and gel purified ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from tissue samples using the Monach Total RNA kit (NEB) and assessed for quality using RNA Integrity Number (RIN ...
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bioRxiv - Immunology 2024Quote: ... following the protocol “High Molecular Weight DNA (HMW DNA) Extraction from Cells (NEB #T3050)” with the following alterations ...
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bioRxiv - Microbiology 2024Quote: Bacterial DNA or lysed cell suspension was qPCR amplified using Phusion polymerase from NEB according to the manufacturer’s instruction using the following thermocycling protocol ...
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bioRxiv - Microbiology 2024Quote: DNA from phage 6E351A was purified using the Monarch® RNA Cleanup Kit (NEB), and 0.5 µg of DNA was hydrolyzed with a nucleoside digestion mix (NEB) ...
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bioRxiv - Neuroscience 2024Quote: ... Amplicons were purified from PCR buffer using Monarch PCR cleanup kit (New England Biolabs) and submitted for Sanger sequencing (EtonBio).
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bioRxiv - Bioengineering 2024Quote: ... 1 µL of T4 ligase and 0.5 µL of T4 polymerase (both from NEB) were added to 20 µL of PBS ...
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bioRxiv - Molecular Biology 2024Quote: For lambda phosphatase assay the reactive from Lambda Protein Phosphatase (Lambda PP) (P0753L, NEB) were used and followed the protocol from the manufacturer.
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bioRxiv - Microbiology 2024Quote: ... after PCR amplification from Bacteroides fragilis ATCC 25285 (NCTC 9343) genomic DNA by NEB HiFi DNA assembly (Gibson Assembly) ...
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bioRxiv - Pathology 2024Quote: ... Total RNA was extracted from cells using Monarch Total RNA Miniprep Kit (NEB, T2010S) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... we added 5 pg of unmethylated DNA from phage λ (New England Biolabs, E7123A) per 10 ng sample DNA ...
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bioRxiv - Neuroscience 2024Quote: ... biotin was removed from non-ligated DNA ends in a T4 polymerase (NEB, M0203L) reaction and again subjected to phenol-chloroform purification ...
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bioRxiv - Biophysics 2024Quote: ... FUS-GFP was cleaved from MBP using TEV protease with a His tag (NEB) at 30 °C for 2 hours and collected as wash through on Ni-NTA beads ...
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bioRxiv - Biochemistry 2024Quote: All proteins were purified from standard pET28a vectors expressed in BL21DE3 cells (NEB, C2527H). FoTO1 and FoTO1ΔCterm were purified as C terminal fusions ...
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bioRxiv - Genetics 2024Quote: ... 5 μL NEB T4 DNA Ligase from the NEB Quick Ligation Module (NEB, E6056), and 1.0 μL Native Adapter (NA ...
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bioRxiv - Genetics 2020Quote: ... We amplified hmx1 sequence from the cDNA using Phusion High-Fidelity DNA Polymerase (M0530L, NEB), primers hmx1-FW ...
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bioRxiv - Molecular Biology 2021Quote: ... The abundant rRNAs were depleted from the sample using NEBNext rRNA Depletion Kit (NEB, #E6310). The rRNA-depleted RNAs were then subjected to RNA-seq library construction using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... All enzymes and related buffers were purchased from New England Biolabs (NEB, Ipswich, MA, USA) unless otherwise indicated ...
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bioRxiv - Biochemistry 2020Quote: N-linked glycans were released from gp140 in-gel using PNGase F (New England Biolabs). The released glycans were subsequently fluorescently labelled with procainamide using 110mg/ml procainamide and 60mg/ml sodium cyanoborohydride ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... poly (ADP-ribose) polymerase (PARP) (both from Cell Signaling via New England Biolabs, Frankfurt, Germany), MYCN (abcam ...
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bioRxiv - Genomics 2020Quote: KlenTaq wild type gene was amplified using a high fidelity PCR (Q5 polymerase from NEB) from a stored plasmid ...
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bioRxiv - Biophysics 2021Quote: A 2.5 kbp DNA fragment was PCR amplified from the pUC19 plasmid (New England Biolabs) with a double digoxigenin-labeled primer (Biomers GmbH ...
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bioRxiv - Genetics 2021Quote: All PCRs were performed with Q5 High-Fidelity polymerase from New England Biolabs (NEB, M0492L). Standard subcloning protocols were used to generate all the DNA plasmids (see details below) ...
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bioRxiv - Genomics 2021Quote: ... rRNA was removed from 3 replicates with NEBNext Bacteria rRNA Depletion Kit (New England Biolabs), while the other 3 were treated with NEBNext Depletion Core Reagent Set using custom probes targeted to Haloferax volcanii rRNA (Martinez-Pastor and Sakrikar ...
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bioRxiv - Microbiology 2021Quote: ... RNA was then removed from the mixture by incubation with 5U RNase H (NEB, USA) and 10μg DNase-free RNase A (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... and T7 RNA polymerase from HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB). Cold-anesthetized female mosquitoes were microinjected with 2.0 µg gene-specific dsRNA using a Nanoject II microinjector (Drummond Scientific Company ...
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bioRxiv - Biochemistry 2021Quote: ... which was purchased from Integrated DNA Technologies and end-labeled with T4 polynucleotide kinase (NEB) and γP32-ATP (6000 Ci/mmol ...
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bioRxiv - Bioengineering 2021Quote: ... DNA extracted from the 30mL culture was digested with HindIII-HF (#R3104S, New England Biolabs) and BamH1-HF (#R3136S ...
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bioRxiv - Bioengineering 2021Quote: Site directed mutagenesis was performed using Q5® Site-Directed Mutagenesis Kit from NEB (E0554S) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Library construction was performed using the NEBNext UltraII DNA Library Prep Kit from NEB (E7645S). Indexed samples were run using the Illumina Next-seq 300-cycle kit ...
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bioRxiv - Synthetic Biology 2021Quote: ... gBlocks were amplified by PCR using Q5 High-Fidelity DNA Polymerase from NEB (NEB #M0492L) and NEB Tm calculator tool was used to estimate the annealing temperature ...
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bioRxiv - Microbiology 2021Quote: ... using a 12-µL Gibson Assembly (GA) reaction (purchased from NEB or prepared in house) by incubation at 50°C for 1 h ...
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bioRxiv - Plant Biology 2020Quote: ... MBP and GST fusion proteins were separated from supernatants using amylose resin (New England Biolabs) and Glutathione Sepharose 4 Fast Flow (GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was generated from the pHH21-NA flag plasmids with Q5 Hot-Start PCR (NEB). This cDNA product was then cloned into the mammalian expression vector pCAGGS for transient transfection experiments as previously described [51].
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bioRxiv - Cell Biology 2020Quote: For cloning standard restriction enzyme digests or oligo annealing and ligations using enzymes from NEB was used ...
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bioRxiv - Synthetic Biology 2021Quote: ... Enzyme digestions were performed using high-fidelity restriction enzymes purchased from New England Biolabs (NEB). DNA ligations were performed using Promega T4 ligase ...
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bioRxiv - Molecular Biology 2021Quote: WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA) was purchased from NEB (MA, USA). The iNAAT reaction volume was 15 μl ...
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bioRxiv - Cell Biology 2021Quote: ... from all the samples and amplified by PCR using Q5 master mix (New England Biolabs) and forward-TGTGTCAATGGAACTCTTGAC and reverse-TCGTTTCGACACCGAAGCAG primers.
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bioRxiv - Biophysics 2020Quote: ... 2.5 kbp and 1.3 kbp DNA tethers were generated by PCR from pUC19 plasmid (NEB) with a double digoxigenin- or biotin-labeled primer on one side and a phosphoprimer on the other side ...
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bioRxiv - Bioengineering 2022Quote: cDNA was synthesized from RNA using the Protoscript II First Strand cDNA Synthesis Kit (NEB). qPCR was performed using a CFX Connect Real Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Developmental Biology 2022Quote: ... total RNA (1 µg) extracted from adult mouse testes was treated with alkaline phosphatase (NEB), de-phosphorylated ...