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Citations for New England Biolabs :
701 - 750 of 3027 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... Phusion DNA polymerase and T5 exonuclease were purchased from New England BioLabs (NEB). Sybr Safe DNA gel stain and BenchMark™ His-tagged Protein Standard were purchased from Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... The ‘601’ fragments were released from the plasmid by digestion with EcoRV (NEB), isolated by incremental PEG precipitation ...
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ISL2 is an epigenetically silenced tumor suppressor and regulator of metabolism in pancreatic cancerbioRxiv - Molecular Biology 2020Quote: ... Q5-high Fidelity 2× master mix was used as polymerase from NEB (# M0429L). PCR condition for the 1st PCR was ...
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bioRxiv - Genetics 2021Quote: ... mRNA was generated from the linearized plasmids with the T3 RNA polymerase (NEB). 200 pg of wild-type or mutant pola2 mRNA were injected into embryos from a cross at the 1-cell stage ...
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bioRxiv - Evolutionary Biology 2021Quote: ... including removal of DNA from the column with DNase I (New England Biolabs). Total RNA was quantified with Qbit 3.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... Phusion polymerase (M0532) and T4 DNA ligase (M0202) were also obtained from NEB. Plasmid mini-prep kit (Cat ...
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bioRxiv - Neuroscience 2021Quote: ... Glycosylation was removed from denatured extracted lysates using PNGase F (NEB, Cat# P0704S) as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary antibodies used were from Cell Signalling Technologies (New England BioLabs, ON, CAN). They included phosphorylated and total extracellular signal-regulated kinase (P-ERK 1/2 ...
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bioRxiv - Immunology 2022Quote: ... N-glycans were enzymatically removed from the underlying peptides with PNGase F (NEB) overnight at 37 °C ...
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bioRxiv - Physiology 2020Quote: ... according to the manufacturer’s recommendations (Magnetic mRNA Isolation Kit protocol from NEB, S1550S), and eluted in 17 μL ...
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bioRxiv - Biochemistry 2020Quote: ... Ribonucleotides (ATP, CTP, GTP, and UTP) were purchased from New England Biolabs (NEB). [α–32P] ATP (3000 Ci/mmol ...
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bioRxiv - Genomics 2021Quote: ... XP12 and T4gt genomic DNA used in this study were obtained from NEB.
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bioRxiv - Bioengineering 2021Quote: ... PCR amplicons were generated from plasmid templates using Q5 Hot Start Polymerase (NEB) and pooled into 100 µl reactions for Solid Phase Reversible Immobilization (SPRI ...
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bioRxiv - Genetics 2021Quote: ... each nuclear preparation was washed with 500 μl 1X CutSmart buffer from NEB and spun at 800g for 10 min at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... and restriction enzymes were purchased from New England BioLabs (NEB, Ipswich, MA, USA) or Thermo Fisher Scientific (Waltham ...
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bioRxiv - Biochemistry 2020Quote: ... bound RNA was eluted from the beads using Proteinase K (New England Biolabs) at 550C for 15 mins in modified Blob100 buffer (in the presence of 0.5% (w/v ...
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bioRxiv - Biophysics 2021Quote: ... Cloning and mutagenesis enzymes and primers were purchased from New England BioLabs (NEB) and Integrated DNA Technologies (IDT) ...
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bioRxiv - Cell Biology 2021Quote: Myc- and DDK (FLAG)-tagged Hemopexin mRNA was transcribed from an AgeI (NEB) linearised plasmid using T7 RNA polymerase (Promega) ...
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bioRxiv - Synthetic Biology 2021Quote: ... restriction enzymes and Gibson assembly mix were obtained from New England Biolabs (NEB) (Ipswich ...
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bioRxiv - Synthetic Biology 2021Quote: Restriction enzymes used for molecular biology were purchased from New England Biolabs (NEB). Synthetic oligonucleotides for cloning and sequencing were purchased from Eurofins Genomics or GENEWIZ ...
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bioRxiv - Plant Biology 2021Quote: ... was amplified from Col-0 cDNA using Q5 High-Fidelity DNA Polymerase (NEB), as recommended by the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... Restriction enzymes and Quick Ligation kits were obtained from New England Biolabs (NEB). Sequences were verified by Sanger sequencing (Genewiz).
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bioRxiv - Microbiology 2021Quote: ... was amplified from genomic DNA by PCR using Q5 polymerase (New England Biolabs) with primers ML4212/ML4213 and cloned into the DHFR-TetO7Sag4 plasmid ...
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bioRxiv - Genomics 2020Quote: ... Plasmid DNA was purified from liquid cultures using DNA miniprep kit (NEB #T1010).
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bioRxiv - Bioengineering 2022Quote: ... coli SHuffle T7 Express lysY and the restriction enzymes were purchased from NEB Japan ...
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bioRxiv - Cancer Biology 2022Quote: ... and cell pellets from our patient-derived cell lines was extracted (NEB, T3010S). Genomic libraries were prepared (Illumina ...
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bioRxiv - Biochemistry 2022Quote: Cell lines and molecular biology materials were purchased from New England Biolabs (NEB) unless otherwise indicated ...
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bioRxiv - Synthetic Biology 2022Quote: ... Restriction enzymes used for vector digests were purchased from New England Biolabs (NEB). Sanger sequencing was performed by Quintara Biosciences (Cambridge ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from single-colony cultures (Monarch Genomic DNA Purification Kit, NEB). Short read libraries were prepared based on the NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads (NEB ...
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bioRxiv - Immunology 2022Quote: ... TCRs were then amplified by PCR (20 cycles with the Phusion from NEB) with a single primer pair binding to the constant region and the adapter linked to the TRAV/TRBV primers added during the reverse transcription ...
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bioRxiv - Molecular Biology 2022Quote: ... The LacO array was excised from the pLAU43_LacO_plus vector42 with XhoI (NEB, R0146L) and ligated into the linearized targeting vector (cut with XhoI ...
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bioRxiv - Microbiology 2022Quote: ... All DNA polymerases and restriction enzymes were purchased from New England Biolabs (NEB) unless stated otherwise ...
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bioRxiv - Bioengineering 2022Quote: ... cas12a (1 μM) and ATP are purchased from NEB (New England Biolabs Inc). SYBR Green II dye ...
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bioRxiv - Cell Biology 2022Quote: ... The standard procedure and reagents from the Q5 Site-Directed Mutagenesis Kit (NEB) were used to insert mutations ...
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bioRxiv - Plant Biology 2021Quote: ... yielding almost 1.6 M mapping-quality variants (homozygous in NEB, absent from WUS). ddRADseq libraries were de-multiplexed and barcodes removed and variants called using a GATK 4.0 best practices pipeline29 ...
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bioRxiv - Developmental Biology 2021Quote: ... Grb10a was amplified from cDNA by high specificity PCR (NEB Q5 Hot Start) with primers flanking open reading frame (Table 1) ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNA from each sample was treated on-column with DNase I (NEB) for 15 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Ribonucleotides (ATP, CTP, GTP and UTP) were purchased from New England Biolabs (NEB). [α–32P] ATP (3000 Ci/mmol ...
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bioRxiv - Bioengineering 2021Quote: ... (4) lacI and tac promoter from pMAL-c5X (New England Biolabs, Ipswich, MA). The modified replacement plasmid for pMut2 ...
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bioRxiv - Systems Biology 2022Quote: ... with the exception of using the commercial φ29 reaction buffer from NEB (B0269L). Amplification products were cleaned and concentrated using the E.Z.N.A.® Cycle-Pure Kit from Omega BioTek (D6492-02 ...
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bioRxiv - Cell Biology 2022Quote: ... pGEM-N-SNAPf was first constructed by removing SNAPf from pSNAPf (NEB, N9183S) with AgeI-XhoI and inserting it into the AgeI-XhoI site of pGEM-HE (51) ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from cells using Monarch total RNA Miniprep kit (NEB). cDNA synthesis was performed using LunascriptTM RT Supermix Kit (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... The pMal-c5X vector was purchased from New England Bioscience (NEB, Ipswich, MA). Tenofovir-DP ...
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bioRxiv - Genomics 2020Quote: Sequencing libraries were prepared using DNA derived from Enterobacteria phage lambda (NEB N3011S), fragmented using a g-TUBE™(Covaris® 520079 ...
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bioRxiv - Biochemistry 2020Quote: ... Restriction enzymes were purchased in their HF format from New England Biolabs (NEB); PCR stages used the KOD HotStart Polymerase Kit (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: PCR was performed using Phusion HF polymerase purchased from New England Biolabs (NEB). T4 polynucleotide kinase (T4 PNK ...
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bioRxiv - Synthetic Biology 2020Quote: ... SNAP reagents (BG-649, BG-fluorescein and BG-biotin) were obtained from NEB. Electrocompetent cells were used in all transformations.
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bioRxiv - Synthetic Biology 2020Quote: ... Enzymes and DNA/RNA ladders were purchased from New England BioLabs® (NEB), and used as per the manufacturer’s instructions unless otherwise indicated ...
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bioRxiv - Immunology 2020Quote: ... RNA was isolated from the cell culture supernatants and converted to cDNA (NEB). The E gene of stock virus ...
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bioRxiv - Synthetic Biology 2021Quote: All restriction enzymes used for molecular biology were from New England Biolabs (NEB). Synthetic oligonucleotides for cloning and sequencing were purchased from Eurofins Genomics or GENEWIZ ...