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Citations for New England Biolabs :
1151 - 1200 of 3027 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The effector AVR-Pii from was obtained via PCR amplification using Phusion Polymerase (New England Biolabs). Primers were designed on the promoter and terminator of ZiF_VIIIc from BTJP 4-1 and included the overhangs required for plasmid assembly ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: Genes were amplified by PCR from a testis cDNA library and digested by EcoR I (NEB) and Nde I (NEB) ...
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bioRxiv - Biochemistry 2024Quote: ... Strep-GRP94 FL was cloned from C2C12 cDNA into pET21d by Gibson assembly (NEB Hifi Builder). His6-BiP-NBD (27-411 ...
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bioRxiv - Cell Biology 2024Quote: ... was used to generate pcDNA3.1-ABDTS from pcDNA3.1 vector digested with EcoRI-HF (Cat #: R310S; NEB)/NotI-HF (Cat # ...
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bioRxiv - Synthetic Biology 2024Quote: ... The HDRT integration site was PCR-amplified from genomic DNA using Q5 High-Fidelity Polymerase (NEB), with an expected amplicon size of ∼1kb for non-edited B2M alleles and ∼2kb for alleles with HDRT integration ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted from ear punch biopsies using the Monarch Genomic DNA purification kit (NEB). Transgenes were identified using PCR primers specific for each transgene (Suppl ...
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bioRxiv - Molecular Biology 2023Quote: ... and the 3,240 bp fragment recovered from the gel (Monarch Gel Extraction Kit, NEB, Ipswich, MA). Components of the transgene construct ...
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bioRxiv - Biophysics 2024Quote: ... and the resulting RNAs were isolated from the reaction mixtures by Monarch RNA Cleanup Kit (NEB). The size and purity of the RNA constructs was confirmed by TBE-urea PAGE (Supplementary Fig ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein samples collected from culture media were treated with PNGase F enzyme (New England Biolabs, P0709S) to remove the N-linked oligosaccharides from the TECTA protein according to the manufacturer protocol ...
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bioRxiv - Microbiology 2022Quote: Phusion polymerase (Thermo) was used for PCR using primers from Microsynth (Switzerland) and restriction enzymes (NEB) for digestion ...
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bioRxiv - Genomics 2022Quote: ... the crosslinked DNA from 10 million nuclei was first digested overnight with 400 U DpnII (NEB) at 37°C ...
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bioRxiv - Biophysics 2022Quote: 10 kilobase long λ-DNA was prepared from full-length phage λ-DNA (New England BioLabs) by performing polymerase chain reaction (PCR ...
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bioRxiv - Bioengineering 2022Quote: ... cDNA synthesis was performed with random primers from the Protoscript cDNA synthesis kit (NEB Cat#E6560S). Transgene expression was then quantified via qPCR using the iTaq Universal SYBR green supermix and primers binding to the mCherry transcript ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Fragments were amplified from the UCA860 sequence via PCR using Q5 Polymerase (NEB, Ipswich, MA, #M0491). The resulting fragments were purified using a 2X ratio of Aline beads (Aline Biosciences ...
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bioRxiv - Genomics 2022Quote: ... polyadenylated RNA was isolated from total RNA using oligo d(T)25 Magnetic Beads (NEB, #S1419S) at a 1μg Total RNA to 10μg beads ratio ...
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bioRxiv - Synthetic Biology 2022Quote: ... The target gene sequences were PCR amplified from the purchased plasmids using either Phusion (NEB, M0531S) or Q5 High Fidelity (NEB ...
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bioRxiv - Microbiology 2022Quote: ... (a kind gift from Prof. William Jacobs Jr.) was double digested with VspI and XhoI (NEB) and the fragment lacking kanR was purified from the agarose gel ...
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bioRxiv - Microbiology 2022Quote: ... The restriction enzymes used were either BsaI or BsmBI (both 10 000 U/mL from NEB).
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bioRxiv - Microbiology 2022Quote: ... diluted in pure water and quantified with the Luna® Universal qPCR Master mix from NEB as previously described.
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bioRxiv - Neuroscience 2022Quote: ... made from pCX with the promoter replaced by human Synapsin (hSyn) using golden gate assembly (NEB). For synaptic labelling pCAG-GPHN-FingR-EGFP-CCR5TC40 Addgene # 46296 ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were eluted from beads with 11 μl 1x TE and 1.5 μl USER enzyme (NEB) for 15 min at 37°C ...
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bioRxiv - Genomics 2022Quote: ... Barcodes were amplified from the library using Q5 High-Fidelity 2X Master Mix (Q5, NEB #M0492) using primers GWLP P2-3 with 20 cycles ...
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bioRxiv - Cell Biology 2023Quote: ... The blasticidin resistance gene was isolated from a plasmid pKM808 using XhoI/SalI (New England Biolabs) and treated with a Quick Blunting Kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was derived from total RNA using ProtoScript II First Strand cDNA Synthesis Kit (NEB, E6560S). Numerous primers were designed against cryptic exon targets and screened to identify primer pairs that minimized background bands.
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bioRxiv - Physiology 2024Quote: ... Plasmid and genomic DNA (gDNA miniprep kits were purchased from Promega. E. coli NEB 5α (NEB) was used as a host for plasmids ...
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bioRxiv - Physiology 2024Quote: ... Plasmid and genomic DNA (gDNA miniprep kits were purchased from Promega. E. coli NEB 5α (NEB) was used as a host for plasmids ...
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bioRxiv - Genetics 2024Quote: ... as well as a number of buffers customarily used for restriction enzyme reactions sourced from NEB. We found that all NEB buffers containing Mg ions were not suitable for the recombination reactions ...
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bioRxiv - Molecular Biology 2024Quote: ... hnRNPA1 effector domain was amplified from HEK293T cDNA using the Q5 High fidelity polymerase (#M0491S, NEB). Overhanging BsWI sites were added to the oligonucleotides for cloning into the dCasRx plasmid with Quick Ligation kit (M2200S ...
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bioRxiv - Cancer Biology 2024Quote: ... the DNA fragments were purified from gel electrophoresis using the Monarch DNA gel extraction kit (NEB), fragments and destination plasmid were digested using the respective restriction enzymes (NEB ...
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bioRxiv - Biophysics 2024Quote: ... were ligated to the product from the preceding step using NEBNext Ultra Ligation Module (NEB #7445L). The resulting product was size-selected as described in the manufacturer’s protocol by employing modified SPRIselect (Beckman Coulter #B23318 ...
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bioRxiv - Systems Biology 2024Quote: ... 66U/μl truncated T4 ligase 2 and 13U/μl murine RNAse inhibitor (all from NEB, USA)) were added at 25°C for 1h ...
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bioRxiv - Synthetic Biology 2024Quote: ... Reagents added were 1 μl of 1 mg/ml BSA (diluted from 20 mg/ml - NEB), 1 μl T4 DNA ligase buffer (ThermoFisher/NEB) ...
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bioRxiv - Plant Biology 2024Quote: First strand cDNA was synthesized from DNase-treated RNA with LunaScript® RT SuperMix Kit (NEB). Prepared cDNA was amplified in Real Time utilizing the StepOnePlus real-time PCR system (Applied Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... The PCR products from MsORF1 cells will be digested by the restriction enzyme BsaAI (NEB, R0531S).
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bioRxiv - Neuroscience 2024Quote: ... and then Gal4DBDZp was replaced with LexADBDZp amplified from UAS-LexADBD76 by Gibson assembly (NEB #E2611S) to generate a pBPLexADBDZp construct ...
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bioRxiv - Genomics 2024Quote: ... Supernatant containing PCR products were separated from beads using 12-tube magnetic separation rack (NEB # S1509S) and purified using 0.9x volume of NEBNext Sample Purification beads ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was then purified from the quenched reaction mixtures using Monarch® RNA Cleanup Kit (NEB) according to manufacturer protocols and subsequently quantified by NanoDrop.
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bioRxiv - Immunology 2024Quote: ... were made using restriction enzymes and T4 DNA ligase from New England Biolabs (NEB, Whitby, ON). The immunoglobulin heavy chain enhancer (EiH ...
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bioRxiv - Cancer Biology 2024Quote: RNA was harvested from cell pellets using the Monarch Total RNA Miniprep Kit (New England Biolabs). Library preparation and sequencing was performed at Washington University School of Medicine GTAC.
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bioRxiv - Genomics 2024Quote: ... 2.67 uL of 10X RNA Fragmentation Buffer from the NEBNext Magnesium RNA Fragmentation Module (E6150S, NEB) was added ...
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bioRxiv - Cancer Biology 2024Quote: RNA was harvested from cell pellets using the Monarch Total RNA Miniprep Kit (New England Biolabs). cDNA was produced using the Invitrogen RNA-to-cDNA kit ...
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bioRxiv - Developmental Biology 2024Quote: RNA extraction from hiPSCs/hESCs was conducted using the Monarch Total RNA Miniprep Kit (NEB T2010S) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... The mRNA was purified from human PAECs using Magnic mRNA isolation kit (New England Biolabs, S1550S). In order to obtain the required quantity of RNA ...
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bioRxiv - Bioengineering 2024Quote: ... for Gibson Assembly and from fragments generated via PCR with Q5 High Fidelity DNA polymerase (NEB). Assemblies were transformed into NEB Stable E ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA was extracted from 5×106 cells using a Monarch Genomic DNA extraction kit (NEB). 2 ng of genomic DNA was incubated at 55°C for 15 minutes with 0.4 ul TDE1 (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... via Ligation Independent Cloning (LIC) or Restriction cloning using enzymes purchased from New England Biolabs (NEB). Full-length IQGAP3 coding sequence was cloned from pCMV-IQGAP3-GFP and inserted into 3xHA-TurboID pRetrox ...
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bioRxiv - Genetics 2024Quote: Genomic DNA was isolated from cell pellets using the Monarch Genomic DNA Purification Kit (NEB, #T3010L) following the manufacturer’s protocol for cultured cells ...
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bioRxiv - Plant Biology 2020Quote: ... RGA::dRGA was amplified from genomic DNA of Col-0 using Q5 high fidelity DNA polymerase (NEB). Two overlapping fragments lacking 17 aminoacids covering the DELLA domain like described in (Feng et al ...
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bioRxiv - Synthetic Biology 2021Quote: ... Linear DNA templates from IDT were amplified by PCR using Q5 High fidelity DNA polymerase (NEB, #M0491S) and dNTPs (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... Total blood RNA from DBS was extracted using Monarch Total RNA Miniprep Kit (New England Biolabs, USA) with a modified protocol ...