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Citations for New England Biolabs :
851 - 900 of 3027 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... Primers were ordered from IDT with Q5 Tms (calculated via the NEB Tm calculator) of 70-72 degrees ...
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bioRxiv - Plant Biology 2021Quote: Recombinant proteins from Escherichia coli lysates were immobilized on amylose resins (New England Biolabs), incubated for 1 h at 4 °C with purified GST-SH3P2 ...
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bioRxiv - Cell Biology 2020Quote: ... using the Q5 quick mutagenesis kit (purchased from New England BioLabs, catalog number E0554s) and following the protocol provided by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... All media components were purchased from BD Difco (Franklin Lakes, NJ) and all enzymes from New England Biolabs (NEB; Ipswich, MA) unless otherwise noted.
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bioRxiv - Developmental Biology 2021Quote: ... purified from 1.5% agarose and quantified with NEBNext Library Quant Kit for Illumina (NEB). Sequencing was performed on Illumina HiSeqV4 SR50.
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bioRxiv - Synthetic Biology 2021Quote: Total RNA was extracted from cell-free expression reactions with a kit (NEB #T2010), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and Bp1026b_II2523 were PCR amplified from Bp82 genomic DNA using Phusion DNA polymerase (NEB) or Kapa HiFi polymerase (KapaBiosystems ...
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bioRxiv - Genomics 2021Quote: ... cDNA was prepared from viral RNA using random primer mix (NEB, Massachusetts, United States), and LunaScript® RT SuperMix Kit (Cat# E3010G) ...
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bioRxiv - Genetics 2020Quote: Repair template plasmids were assembled from overlapping fragments using the NEBuilder HiFi Kit (NEB) per manufacturer instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... mRNA was isolated from total RNA using a Magnetic mRNA isolation kit (S1550S, NEB), and libraries were prepared using a NEXTflex Rapid directional RNA-seq kit (5138-08 ...
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bioRxiv - Bioengineering 2022Quote: RNA was isolated from gut mucosal biopsies using Monarch Total RNA Miniprep Kit (NEB) according to the manufacturers’ protocols ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were designed in SnapGene and mutants were generated via Gibson assembly (Gibson Assembly® Cloning Kit from New England Biolabs)57,58 or via QuickChange (Q5® Site-Directed Mutagenesis Kit from New England Biolabs).59 Recombinant expression of motors in E ...
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bioRxiv - Genomics 2022Quote: ... An isothermal extension reaction mixture was made from 35μl NEBuffer2 (10×, New England Biolabs), 12.5μl Klenow Fragment (5,000U/ml ...
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bioRxiv - Developmental Biology 2022Quote: ... or from COV434 cells and hiPSCs using the Monarch Total RNA Miniprep kit (NEB). For experiments involving TF overexpression ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was removed from the extracted RNA using DNase I (New England Biolabs) treatment and removal was confirmed by PCR of a reference gene ...
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bioRxiv - Plant Biology 2023Quote: ... Real time reactions were performed using Luna® Universal qPCR Master Mix from NEB. Oligonucleotides were used to detect GFP-VAMP (56 and 57 ...
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bioRxiv - Plant Biology 2023Quote: ... the culture filtrates from methanol-induced cultures were treated with PNGaseF (New England Biolabs), and then evaluated by immune-decoration analysis using a monoclonal His-antibody (AbHis ...
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bioRxiv - Molecular Biology 2022Quote: ... treated with phenol-free RNA Binding Buffer (included in RNA Cleanup Kits from NEB) and Proteinase K ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids were isolated from individual colonies (Monarch Plasmid Miniprep Kit, New England Biolabs, #T1010L) and sequenced (Mix2Seq Kit NightXpress ...
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bioRxiv - Microbiology 2022Quote: ... rRNA was removed from the total RNA using a NEBNext rRNA depletion kit (NEB), and RNA was converted to a DNA library using a TruSeq stranded total RNA kit (Illumina) ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA was extracted from collected spermatids fractions with Monarch RNA extraction kit (NEB) according to manufacturers’ protocol for RNA extraction from tissue or leukocytes ...
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bioRxiv - Molecular Biology 2024Quote: ... The purification protocol for the poly(A)+ RNA was optimized from the manufacturer (NEB) as ribosomes have very high content of rRNAs ...
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bioRxiv - Bioengineering 2024Quote: The 5’ RACE protocol using the template-switching reverse transcriptase enzyme mix from NEB was executed following the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNAs generated from CIT9 cell line and Q5® High-Fidelity DNA polymerase (NEB). MSCV 2.2-IRES-CFP retroviral vector was generated by replacing GFP of MSCV 2.2-IRES-GFP vector (obtained from James Carlyle lab at University of Toronto ...
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bioRxiv - Microbiology 2023Quote: Total RNA was isolated from cells using the Monarch Total RNA Miniprep kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... the scFv insert from isolated plasmids was amplified by PCR using Q5 polymerase (NEB). DNA samples were prepped and run using the Illumina MiSeq v3 reagent kit following manufacturer’s protocols ...
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bioRxiv - Cell Biology 2022Quote: ... coli PURExpress system were generated from the T7 PURExpress plasmid (New England Biolabs, USA). pSpCas9(BB)-2A-Puro (PX459 ...
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bioRxiv - Molecular Biology 2023Quote: The single- and double-stranded M13 DNA was obtained from New England Biolabs (NEB). The genomes mixture was generated from the combination of four different bacterial genomes ...
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bioRxiv - Genetics 2023Quote: ... biotin from un-ligated DNA ends was removed using T4 DNA polymerase (NEB #M0203) and the DNA was sonicated (Covaris E220 ...
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bioRxiv - Plant Biology 2023Quote: ... oligo(dT) primed cDNAs were synthesized from total RNA with MMLV (reverse transcriptase, NEB). Reactions of qRT-PCR were set up with 50-70ng of cDNA and 250 nM gene-specific primers using FastStart Universal Sybr Green Master (Rox ...
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bioRxiv - Microbiology 2023Quote: ... was amplified from Ap strain HZ gDNA using Q5 DNA polymerase (New England Biolabs). Cloning into pET-28a and a modified pET41 was performed using restriction free PCR-based methods26 ...
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bioRxiv - Molecular Biology 2023Quote: ... T4- PNK (polynucleotide kinase) and NheI enzymes were purchased from New England Biolab (NEB).
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted from cell pellets using the Monarch gDNA Purification Kit (NEB). 2 µg of purified genomic DNA was then bisulfite converted using the EpiTect Bisulfite Kit (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: ... Genes of interest were amplified from wild-type genomic DNA using Phusion Polymerase (NEB), according to the manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2022Quote: ... and a high-fidelity polymerase (Phusion) purchased from New England Biolabs (NEB, Ipswich, MA). 10 μL PCR reactions were set-up using 2 μL 5x HF Buffer (NEB) ...
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bioRxiv - Genomics 2022Quote: ... RT-qPCR was performed with Luna Universal One-Step RT-qPCR Kit from NEB on BioRad CFX96 Touch Real-Time PCR Detection System ...
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bioRxiv - Cancer Biology 2022Quote: ... Restriction reactions were performed using High-Fidelity Restriction enzymes from New England Biolabs (NEB), by incubating 2 μg of DNA with 2U of each enzyme in the presence of 10X NEB CutSmart buffer ...
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bioRxiv - Biochemistry 2023Quote: ... 5 µg of genomic DNA from the clones were digested solely with XhoI (NEB) overnight at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... cDNA (1 µg) was prepared from total RNA using LunaScript cDNA synthesis mix (NEB), diluted 5 times before using it for qRT-PCR ...
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bioRxiv - Developmental Biology 2023Quote: The TrackerSeq lineage libraries retrieved from cDNA were amplified with Q5 polymerase (NEB, M094S) in a 50-µl reaction ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR was performed using Q5 High-Fidelity DNA polymerase from New England Biolabs (NEB). Genes were either synthesized as bacterial codon-optimized gBlocks fragments (IDT ...
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bioRxiv - Microbiology 2023Quote: ... along with NEBNext Quick Ligation Reaction Buffer and T4 DNA ligase (both from NEB). The reaction incubated for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Ribosomal RNA was depleted from total RNA with the rRNA depletion kit (NEB# E6310) and subsequently prepared for RNA-seq with the NEBNext Ultra Directional RNA Library Prep Kit (NEB #E7420 ...
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bioRxiv - Genetics 2023Quote: ... We performed cDNA synthesis from RNA using LunaScript RT Supermix Kit (New England Biolabs) beginning with 500ng of RNA ...
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bioRxiv - Biophysics 2023Quote: ... Chimeric NICDs were created from native NICDs using HiFi DNA Assembly (New England Biolabs). Boundaries for chimeric constructs are given in Table S3.
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bioRxiv - Cancer Biology 2023Quote: ... and regularly tested for mycoplasma contamination (PCR-based assay from Minerva-Biolabs, Berlin, Germany).
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bioRxiv - Biophysics 2023Quote: ... DNA was purchased from New England Biotechnologies and treated with Klenow fragment polymerase (NEB) and a dNTP mix containing biotinylated dCTP ...
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bioRxiv - Biophysics 2023Quote: ... fasciculata minicircles and maxicircle were predicted for enzymes available from New England Biolabs (NEB) using a custom python script to facilitate enzyme choice.
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bioRxiv - Molecular Biology 2023Quote: DNA from positive clones was extracted using a Monarch Genomic DNA Purification kit (NEB). DNA insertion was confirmed by amplification of a fragment including both genomic and kanamycin resistance cassette sequences followed by the sequencing of amplified DNA (Macrogen).
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bioRxiv - Neuroscience 2024Quote: ... Amplicons were purified from PCR buffer using Monarch PCR cleanup kit (New England Biolabs) and submitted for Sanger sequencing (EtonBio).