Labshake search
Citations for New England Biolabs :
9201 - 9250 of 10000+ citations for Human Oxidized low density lipoprotein receptor 1 OLR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Construction of pUAST- attB-Cic(1-15)A-SBP and pUAST-attB-Cic20A-mVenus was carried out by assembling gene fragments using the HiFi DNA assembly Kit (NEB). Construction of the other subgroups of Cic mutants was done by assembling different parts of Cic generated by overlap PCR using wild type Cic ...
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bioRxiv - Genomics 2024Quote: ... Following end repair with NEBNext® End Prep Ultra II Kit (Supplemental Table 9, Cat No. E7645, NEB, Ipswich, MA), custom iTru adapters were ligated to amplicons with NEBNext® Ultra II Ligation Master Mix (Cat No ...
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bioRxiv - Biochemistry 2024Quote: ... Of the rRNA-depleted samples directional libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) in accordance to the recommended protocol ...
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bioRxiv - Biochemistry 2024Quote: ... 1-5 mL of cell pellet was harvested for plasmid extraction with the Monarch Plasmid DNA Miniprep Kit (New England Biolabs) (T1010) ...
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bioRxiv - Biophysics 2024Quote: ... Plasmid DNA was extracted using the Qiagen Plasmid Plus Midi kit and the library inserts were obtained through double endonuclease digestion (HindIII-HF and NheI-HF, NEB) followed by agarose gel purification achieved by a combined use of centrifugal filter units (Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... 5 ng of ChIP and Input DNA was used to generate ChIP-seq libraries using the NEBNext Ultra DNA library prep kit for Illumina (New England Biolabs, NEB).
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bioRxiv - Cancer Biology 2024Quote: ... The purified CASP3 gene fragment was ligated into the linearized pEGFP-N3 vector using the quick ligationTM kit according to the manufacturer’s instructions (NEB, B2200S). The resulting mixture was transformed into competent DH5α bacteria (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... 1μg RNA was used for sequencing libraries using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to each sample ...
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bioRxiv - Cell Biology 2024Quote: ... and R211K were introduced into the mKO2-RIβ plasmid using a site-directed mutagenesis kit (New England BioLabs, Ipswich, MA). N-terminal His-tagged human RIβ-WT and RIβ-L50R constructs ...
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bioRxiv - Molecular Biology 2024Quote: ... After washing samples were resuspended in 150 µl RNA cleanup binding buffer of the Monarch RNA Cleanup Kit (NEB, T2030S). Samples were incubated for 5 min at RT on a rotating wheel ...
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bioRxiv - Microbiology 2024Quote: High molecular weight DNA for long-read sequencing was extracted using the Monarch HMW DNA extraction kit for tissue (NEB), with the specific protocol for Gram-negative bacteria ...
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bioRxiv - Microbiology 2024Quote: ... Isolation of mRNA was achieved via poly(A) enrichment before library preparation (Ultra II Directional RNA library prep kit, New England Biolabs). Libraries were sequenced on an Illumina NovaSeq with 40M 150bp paired end reads per sample.
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bioRxiv - Microbiology 2024Quote: Genomic DNA purification for use in cloning or whole-genome sequencing was purified using the Monarch HMW DNA Extraction Kit for Tissue (T3060L, New England Biolabs) according to manufacturer’s instruction with one modification ...
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bioRxiv - Microbiology 2024Quote: ... and held at 72°C for 15 minutes before purification by PCR and DNA cleanup kit (New England Biolabs, T1030S) according to manufacturer instructions with the following modifications ...
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bioRxiv - Microbiology 2024Quote: Libraries for whole transcriptome sequencing were produced from 20 ng total RNA per sample using NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) and its corresponding sample indexing kit (Index Primer Set 1) ...
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bioRxiv - Microbiology 2024Quote: ... The resulting PCR fragments were combined to generate plasmid pTkv141 using the NEBuilder HiFi DNA Assembly Kit (New England Biolabs), and transformed into chemically competent DH5 E ...
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bioRxiv - Microbiology 2024Quote: ... Library preparation with indexing was performed by the University of Kansas Genome Sequencing core facility with NEB Next RNA Library kit (NEB). RNAseq was performed using an Illumina NextSeq2000 high-output system with paired-end reads of 50 bp ...
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bioRxiv - Bioengineering 2024Quote: ... PolyA selection and stranded RNA-seq library preparation were performed with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760; New England Biolabs). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... then 40 μL of each sample was used reactions as outlined in the manufacturer’s protocol for denaturing reaction conditions using the Protein Deglycosylation Mix II Kit (New England Biolabs, P6044) both with and without deglycosylase enzymes ...
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bioRxiv - Microbiology 2024Quote: ... NEBNext Ultra II DNA Library Prep Kit for Illumina with NEBNext Multiplex oligos for Illumina (NEB cat # E7645S/L; E6440) following the instruction manual (V6.1_5/20 ...
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bioRxiv - Immunology 2024Quote: ... Libraries were prepared from rRNA depleted samples using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) and sequenced on the Novaseq 6000 S4 (Illumina) ...
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bioRxiv - Molecular Biology 2024Quote: Visible LEAP bands were excised from agarose gels and cDNAs were purified using the Monarch DNA Gel Extraction Kit (NEB). Purified DNAs from excised gel slices were cloned into a pCR4Blunt-TOPO vector using a Zero Blunt TOPO PCR Cloning Kit for Sequencing (ThermoFisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... ΔPYD and HD mutants were generated from full length IFI207-HA plasmids and the R1-ΔPYD mutant from R1-IFI207 using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). IFI207-HIN plasmids were generated by PCR amplification of the HIN domain from full length IFI207-V5 expression plasmids ...
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bioRxiv - Microbiology 2024Quote: ... Illumina sequencing libraries were generated using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: Codon-optimized full-length SARS-CoV-2 S protein gene was cloned into Topo cloning vector and 22 N-glycosylation sites were mutated individually from N to Q using a Q5 Site-Directed Mutagenesis Kit (NEB). The full-length or C19 truncated fragments were amplified and inserted into plvx-puro vector ...
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bioRxiv - Microbiology 2024Quote: ... the construct generated above with the WT rel allele in pIMAY (pRel-swap, Table S2) was used for site directed mutagenesis with the Q5-Site-Directed-Mutagenesis kit (NEB) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2024Quote: ... and its variant CjeNThr69Ser77Thr111his6 were cloned into pET21a(+) plasmid using a NEBuilder HiFi DNA assembly cloning kit (New England Biolabs). The proteins were overexpressed in E ...
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bioRxiv - Molecular Biology 2024Quote: ... and Rpa2WH (Rpa2178-268) were cloned from the pRSFduet(+) constructs using the Q5 site directed mutagenesis kit (E0554, New England Biolabs).
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bioRxiv - Microbiology 2024Quote: ... the RNA pellet was resuspended in 50 µl of RNase-free H2O and 1 µl of the resuspended samples was used for quantification of the DENV-2 D220 NS5 RNA regions using Luna Universal One-Step RT-qPCR Kit (New England Biolabs) in a 10-ul reaction volume ...
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bioRxiv - Microbiology 2024Quote: ... mutagenic primers were designed using the New England Biolabs online software NEBaseChanger (primer sequences are available at doi: XXXX. The Q5 Site-Directed Mutagenesis Kit (New England BioLabs) was then used with the A/Baltimore/R0675/2019 hemagglutinin plasmid to produce plasmids with the desired mutations ...
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bioRxiv - Neuroscience 2024Quote: ... Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reversely transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530 ...
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bioRxiv - Molecular Biology 2024Quote: ... Library preparation was performed using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760S/L). The workflow was adapted to generate longer library fragments by reducing the fragmentation time (see manual ...
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bioRxiv - Molecular Biology 2024Quote: ... The 3′ adapter was conjugated with an amino CA linker instead of dCC at the 3′ end (GeneDesign) and then adenylated at the 5′ end with a 5′ DNA adenylation kit (NEB). Four random nucleotides were added in the 3′ and 5′ adapters [(5′ -rAppNNNNTGGAATTCTCGGGTGCCAAGG/amino CA linker-3′ ...
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bioRxiv - Molecular Biology 2024Quote: ... These three DNA fragments were further assembled using the NEBuilder HiFi DNA Assembly cloning kit (New England BioLabs, Ipswich, MA).
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bioRxiv - Molecular Biology 2024Quote: ... DNA fragments were subject to end-repair and dA-tailing with NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs), followed by ligation with 50 pmol of adaptor 1 (Ad1 ...
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bioRxiv - Microbiology 2024Quote: ... This dimer was introduced into pBECAb-apr using the NEBridge Golden Gate Assembly Kit (New England Biolabs, Ipswich, MA, USA) to generate pBECAb-apr-kmtA ...
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bioRxiv - Molecular Biology 2024Quote: ... Modifications of uORFs or other mutations were performed using the Q5 site-directed mutagenesis kit (Cat. No. E0554S, New England Biolabs) and validated by Sanger sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... ChIP-seq libraries were generated using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB # E7645S) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 cycles on a Covaris E220 focused ultrasonicator and were subsequently processed using NEBNext Enzymatic Methyl-Seq kit (NEB #7120) following the manufacturer protocol ...
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bioRxiv - Molecular Biology 2024Quote: Plasmid pHERD30t with and without overlapping AluI/BREX site was purified from BREX+ (E. coli HS) or BREX- cells (E. coli BW25113) using Monarch Plasmid Purification Kit (NEB). 100 ng of plasmid was restricted with AluI (ThermoScientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Introduction of the overlapping BREX/AluI site to the pHERD30t vector was also achieved via Q5 site-directed mutagenesis kit (NEB). Plasmids were transformed into chemically competent XL1-Blue cells (Evrogen) ...
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bioRxiv - Molecular Biology 2024Quote: 50-100 ng of purified PCR product was prepared for sequencing using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Molecular Biology 2024Quote: 175-225 ng of purified PCR product was prepared for sequencing using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Molecular Biology 2024Quote: 200 ng of pooled PCR product was prepared for sequencing using the NEB-Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Genomics 2024Quote: ... libraries were generated from 250 ng of DNase-treated RNA with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) following the Poly(A ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA was converted into Illumina-compatible libraries with the NEBNext(R) Ultra(TM) II DNA Library Prep Kit (NEB E7645L) following the protocol described by 41 and using NEBNext Dual Index Multiplex Oligos (NEB E7600S) ...
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bioRxiv - Genetics 2024Quote: ... with constructs cloned in place of GFP via the Gibson assembly method using the NEBuilder HiFi Cloning Kit (NEB, #E5520S). GPS lentiviral vectors encoding the N-terminus of PTGS1 and the C-terminus of TNNC2 fused to GFP were gifts from Stephen Elledge ...
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bioRxiv - Synthetic Biology 2024Quote: ... where separately introduced to the wild-type MS2 coat protein present in these mRNA constructs using the NEB-Q5 mutagenesis kit (New England Biolabs). The primer sets and PCR settings used are listed in supplementary information ...
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bioRxiv - Synthetic Biology 2024Quote: ... These synthesized fragments were assembled into the Level 0 entry vector pYTK001 using the NEBridge Golden Gate Assembly Kit (BsmBI-v2) (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... rRNA-depleted RNA was used to generate the RNA-sequencing libraries using NEB Ultra II directional kit (New England Biolabs). Samples from Keratinocytes were multiplexed and sequenced on a HiSeq4000 PE 150 sequencing platform (Illumina) ...