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Citations for New England Biolabs :
9401 - 9450 of 10000+ citations for Human Oxidized low density lipoprotein receptor 1 OLR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: Illumina sequencing libraries were prepared from 1ng to 10ng of purified CUT&RUN DNA using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs # E7645) as previously (Liu et al ...
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bioRxiv - Biochemistry 2020Quote: ... PCR products were cloned into pET22b-mCherry vector using the NdeI and SpeI restriction sites and joined using Gibson Assembly® Cloning Kit (New England Biolabs) according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... to obtain the amplified PCR product which was then purified (Quiagen Gel purification kit) and digested with Nco1 and BamH1 restriction endonucleases (NEB, India) for inserting the gene into the pTrc99A expression vector ...
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bioRxiv - Biophysics 2021Quote: ... Mutagenic PCR to obtain the D614G amino acid change into both untagged and 161/345A4-tagged SΔTM constructs was done using the primers S2_D614_Q5-F and S2_D614_Q5-R (Table S1) and the Q5® Site-Directed Mutagenesis Kit (NEB®, Ipswich, MA, USA) according to the manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were prepared using with 1 μg of total RNA and mRNA isolation step was done by using NEBNext poly(A) mRNA Magnetic Isolation kit (New England Biolabs, E7490S). The cDNA libraries were generated by the “dUTP” method for strand-specificity using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... and the DNA-free samples consisting of RNA were converted to cDNA using M-MuLV Reverse Transcriptase kit (New England BioLabs, USA) according to the instructions provided by the supplier ...
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bioRxiv - Cell Biology 2021Quote: ... RNA sequencing libraries were prepared using the NEB Next Ultra RNA Library Prep Kit for Illumina using manufacturer’s instructions (NEB, Ipswich MA). The sequencing library was validated on the Agilent Tape Station (Agilent Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... sgRNAs were chosen to minimize predicted off-target effects with maximum cleavage efficiency and were synthesized by in vitro transcription as previously described26 using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, E2040S) or ...
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bioRxiv - Cancer Biology 2020Quote: ... The Nup88 construct lacking the coiled coil domain (Nup88∆C) was created by inserting a stop codon after 584aa through site directed mutagenesis (Q5 Site Directed Mutagenesis Kit, NEB-E0554S) using pEGFPC1-Nup88 as template ...
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bioRxiv - Cancer Biology 2020Quote: ... was digested with BbsI and the gRNAs targeting E6 and E7 genes from HPV18 and HPV16 were designed using NEBuilder® HiFi DNA Assembly cloning kit (NEW ENGLAND BioLabs). Briefly ...
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bioRxiv - Cell Biology 2021Quote: The components of both cassettes were first PCR-amplified from other vectors and subsequently ligated together using the NEBuilder® HiFi DNA Assembly Cloning kit (NEB) to generate the resulting pENTR-eGFP-attP(bxb)-*BsdR and pENTR-mCherry-attP(C31)-*BleoR vectors ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The single-stranded labeled probes were finally cleaned up using the Monarch PCR & DNA cleanup kit (New England Biolabs®, T1030) following the oligonucleotide cleanup protocol ...
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bioRxiv - Neuroscience 2020Quote: ... RNA was stored short-term at −20°C or long-term at −150°C or immediately used in a retrotranscription reaction (ProtoScript® AMV First Strand cDNA Synthesis Kit, New England Biolabs or SuperScript® III First-Strand Synthesis System ...
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bioRxiv - Neuroscience 2021Quote: ... Point variants were introduced into hFibcd1 cDNA while cloned in the intermediate pDONR201 plasmid using the Q5 site-directed mutagenesis kit (NEB #E0554), following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs, Ipswich, MA, USA inc.) for Illumina following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... Quantification of libraries prior to sequencing used qPCR with primers specific to the Illumina P5 and P7 adaptor sequences and standards from the NEBNext Library Quant Kit (NEB, E7630S). Sequencing of prepared libraries was performed using an Illumina MiniSeq with the 75-cycle high-output kit ...
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bioRxiv - Cell Biology 2021Quote: ... libraries were prepared from purified DNA using “NEBNext Ultra II DNA Library Prep Kit for Illumina” from New England BioLabs (NEB, #E7645), as per the manufacturers’ instructions ...
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bioRxiv - Plant Biology 2020Quote: 20-33 kD subregions of NRPD1 and RDR2 polypeptides were expressed in vitro using a PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs). Briefly ...
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bioRxiv - Synthetic Biology 2021Quote: ... The plasmid of pAC-BETAipi-ispA/dxs was constructed by using Gibson assembly kit (Gibson Assembly® Master Mix, New England Biolabs). The insert was amplified from genomic DNA of E ...
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bioRxiv - Synthetic Biology 2021Quote: ... was linearized by PCR with oligos designed to flank the metY gene (Table S5) and the modified metY(AAC) gene was then inserted using homologous recombination (NEBuilder HiFi DNA Assembly kit (NEB#E2621)) ...
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bioRxiv - Systems Biology 2021Quote: ... using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Plant Biology 2021Quote: ... was used as input for library preparation using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs Inc). Libraries were sequenced using Illumina NextSeq High Output 75 bp pair-end (Illumina Inc. ...
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bioRxiv - Microbiology 2021Quote: Synthesis of RNA in vitro was carried out as previously described using HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, E2050) [35 ...
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bioRxiv - Genetics 2020Quote: ... Followed by a Dual index library prep with the DNA NEBNext® Ultra™ II Library Prep Kit (New England Biolabs), using a quarter of the recommended volumes with the Dual Index Primers Set ...
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bioRxiv - Genetics 2021Quote: ... Libraries for sequencing were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB E7645), as described by the manufacturer but using half volumes of all reagents and starting material ...
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bioRxiv - Microbiology 2021Quote: ... Mutant of the dGTPase gene of Shewanella putrefaciens CN-32 was constructed using Q5 Site directed Mutagenesis kit (NEB, cat. #E0554S) using primers presented in Supplementary Table S5.
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bioRxiv - Microbiology 2020Quote: ... qRTPCR was performed with primers mentioned in Table S3 with Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs). Log2 normalized RNA abundances were calculated using ValidPrime signal as reference (51).
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bioRxiv - Microbiology 2021Quote: ... and fifteen repeats of the FG-repeats were amplified from genomic DNA using primers (Table S2) with Phusion High-Fidelity PCR Kit (NEB - E0553S). The PCR products were purified with GenElute PCR Clean-Up Kit (Sigma-Aldrich NA1020) ...
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bioRxiv - Microbiology 2021Quote: ... while the Zymo Mock sample was prepared with NEB Next Ultra II DNA library prep kit for Illumina (New England Biolabs, USA) following the manufacturer’s protocols and sequenced using the Illumina MiSeq platform.
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed either directly on the inactivated supernatants or on extracted RNA using the Luna Universal One-Step RT-qPCR Kit (NEB #E3005E) in a QuantStudio 6 thermocycler (Applied Biosystems ...
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bioRxiv - Biochemistry 2021Quote: The gene encoding the C-terminal 6xHis GlcNAc-1-kinase NahK from Bifodobacterium longum JCM1217 was inserted into a pBAD/HisB backbone using a NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs) following manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: Double-stranded single-indexed libraries were prepared using NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (NEB #E7645S) and NEBNext® Multiplex Oligos for Illumina® Index Primers Set 1 and 2 (NEB #E7335S ...
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bioRxiv - Microbiology 2021Quote: ... and all of the point mutants were constructed using Mut Express II Fast Mutagenesis Kit V2 (Vazyme, C214) and DpnI endonuclease (NEB, R0176S). Primers used for plasmids construction were listed in Supplementary table 1 and table 2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Pre-miRNAs were purified on denaturant polyacrylamide gel and long pri-miR-K10/12 derived transcripts (up to ∼3 kb) were salt purified using Monarch® PCR and DNA cleanup kit (New England BioLabs). After acidic phenol extraction and ethanol precipitation ...
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bioRxiv - Microbiology 2021Quote: ... EF1α Lamp1-mCherry plasmid was extracted from successful transformants with the Monarch® Plasmid Miniprep Kit (New England BioLabs Inc., USA), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... was used to generate RNA-seq library using NEBNext Ultra Directional RNA library Prep Kit for Illumina (New England BioLabs, Inc) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... were used to construct the RNA-seq libraries using a NEB Next Ultra RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Entry vectors for Golden Gate cloning were made by digesting the Greengate Kit [56] entry vector plasmids (PGGA000, PGGB000, PGGC000, PGGD000, PGGE000, PGGF000) with BSA1-HF V2 (New England Biolabs Inc.). Bladderwort sequences were synthesized (IDT ...
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bioRxiv - Plant Biology 2021Quote: ... The pooled PCR reactions were purified with the Monarch® PCR & DNA Cleanup Kit (5 μg) (New England Biolabs® Inc.) and run on 6% acrylamide gel ...
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bioRxiv - Synthetic Biology 2021Quote: ... All the three fragments were assembled uisng the NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs, Ipswitch, MA, USA) and transformed into E ...
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bioRxiv - Systems Biology 2021Quote: ... Sequencing libraries were generated from the qualified DNA samples(s) using the NEB Next Ultra DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were generated following manufacturer’s recommendations with NEB Next® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA). The constructed libraries were validated using an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... (Leiden, The Netherlands) using the NEBNext® Ultra™ II FS DNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts, USA) and the NextSeq 500 platform (paired-end ...
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bioRxiv - Molecular Biology 2020Quote: ... ATG-free versions of the truncated OpIE2 promoter sequence (tIE2 promoter with no ATG triplet in its sequence) were created by Q5 Site-Directed Mutagenesis (SDM) Kit (New England Biolabs-NEB, USA). Primers used to generate mutations were designed using the NEBaseChanger tool (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... to lysine (K) at 363 (E363K) mutant of HNF4A2 was generated using Q5® Site-Directed Mutagenesis Kit (New England Biolabs). The pcDNA3-SHP-Flag vector for SHP was synthesized by GenScript ...
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bioRxiv - Microbiology 2020Quote: ... and libraries for RNAseq were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The 5’ arm and LexA:p65 coding sequence was assembled from 2 fragments from PCR-amplified Drosophila genome and a LexA:p65 coding sequence using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, cat#E5520), and inserted into XhoI-SpeI sites of the pHD-DsRed plasmid ...
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bioRxiv - Cancer Biology 2020Quote: ... and gene expression was analyzed by RT-qPCR on QuantStudio 6 (Applied Biosciences) using the Luna Universal qPCR kit (New England Biolabs, M3003). Relative expression was normalized to Actb and Gapdh housekeeping genes and was determined using the ΔΔCt method ...
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bioRxiv - Cancer Biology 2020Quote: ... The GST tag in this construct was replaced by a 6xHis tag using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs). N-PTEN was recombinantly expressed in E ...
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bioRxiv - Cell Biology 2021Quote: ... 100 ng total RNA per 20 µl reaction were analyzed using the Luna Universal Probe One-Step RT-qPCR Kit (NEB, # E3006) in technical triplicates ...