Labshake search
Citations for New England Biolabs :
9151 - 9200 of 10000+ citations for Human Oxidized low density lipoprotein receptor 1 OLR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... The Ct values of samples were determined by quantitative PCR (qPCR) using a Luna Universal Probe One-Step RT-qPCR Kit (cat. #E3006, New England Biolabs) with LightCycler 480 (Roche Diagnostics ...
-
bioRxiv - Microbiology 2023Quote: ... The amount of RNA template was equalized for reverse transcription using the Protoscript II First Strand cDNA Synthesis Kit (New England Biolabs) and random hexamers ...
-
bioRxiv - Microbiology 2023Quote: ... Library preparation was conducted by the standard ≥100 ng protocol from the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) with minor modifications ...
-
bioRxiv - Microbiology 2024Quote: Libraries for whole transcriptome sequencing were produced from 20 ng total RNA per sample using NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) and its corresponding sample indexing kit (Index Primer Set 1) ...
-
bioRxiv - Microbiology 2024Quote: ... The resulting PCR fragments were combined to generate plasmid pTkv141 using the NEBuilder HiFi DNA Assembly Kit (New England Biolabs), and transformed into chemically competent DH5 E ...
-
bioRxiv - Microbiology 2024Quote: ... NEBNext Ultra II DNA Library Prep Kit for Illumina with NEBNext Multiplex oligos for Illumina (NEB cat # E7645S/L; E6440) following the instruction manual (V6.1_5/20 ...
-
bioRxiv - Plant Biology 2024Quote: ... The PCR products from divergent primers of expected length were excised and purified via PCR clean up kit (New England Biolabs) for confirmation of BSJ using Sanger Sequencing.
-
bioRxiv - Microbiology 2024Quote: ... rRNA-depleted samples were prepared for next-generation sequencing using NEBNext Ultra II Directional RNA Library Prep kit (NEB; E7765S) and NEBNext Multiplex Oligos for Illumina (NEB ...
-
bioRxiv - Genetics 2024Quote: ... with constructs cloned in place of GFP via the Gibson assembly method using the NEBuilder HiFi Cloning Kit (NEB, #E5520S). GPS lentiviral vectors encoding the N-terminus of PTGS1 and the C-terminus of TNNC2 fused to GFP were gifts from Stephen Elledge ...
-
bioRxiv - Genetics 2024Quote: Golden Gate Assembly was performed largely according to the manufacturer protocol included with the NEBridge Golden Gate Assembly kit (New England Biolabs) using the with alterations noted below (Potapov et al ...
-
bioRxiv - Developmental Biology 2024Quote: ... DNA was converted into Illumina-compatible libraries with the NEBNext(R) Ultra(TM) II DNA Library Prep Kit (NEB E7645L) following the protocol described by 41 and using NEBNext Dual Index Multiplex Oligos (NEB E7600S) ...
-
bioRxiv - Genetics 2024Quote: ... the Cas9 terminator (TCYC1) was switched to an ADH1 terminator (TADH1) by Gibson assembly using the HiFi DNA Assembly kit (New England Biolabs). Cas9D10A ...
-
bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were then prepared from 20ng of input DNA using the NEBNext Ultra-II DNA kit (New England Biolabs) following manufacturer’s instructions22 ...
-
bioRxiv - Neuroscience 2024Quote: ... was used to construct sequencing libraries using the NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs, NEB). Libraries were multiplexed using NEBNext Multiplex Oligos for Illumina (dual index primers ...
-
bioRxiv - Genomics 2024Quote: Double-stranded DNA libraries were prepared with NEBNext Ultra II DNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (New England Biolab: NEB). Around 1 ng of DNA was used for each library ...
-
bioRxiv - Microbiology 2024Quote: ... Genomic DNA libraries were generated using the NEBNext Ultra II FS DNA Library Prep kit for Illumina (New England BioLabs) in combination with NEBNext multiplex oligos for Illumina (96 Unique Dual Index Primer Pairs ...
-
bioRxiv - Microbiology 2024Quote: ... Sequencing libraries for RNA-Seq were constructed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760S), following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Germany). The size distribution of the libraries was checked by high-resolution gel electrophoresis with a Bioanalyzer instrument using the DNA 7500 Pico kit (Agilent Technologies) ...
-
bioRxiv - Genomics 2024Quote: ... was used as the original tissue sample for genomic DNA extractions with Monarch High Molecular Weight (HMW) Extraction Kit for Tissue (New England Biolabs). Spleen tissue (totaling 25 mg ...
-
bioRxiv - Immunology 2024Quote: ... Gene expression levels were quantified by reverse transcription quantitative PCR (RT-qPCR) using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs), and being conducted on a QuantStudio 3 Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Genomics 2024Quote: ... libraries were generated from 250 ng of DNase-treated RNA with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) following the Poly(A ...
-
bioRxiv - Cancer Biology 2024Quote: ... indel assay was performed by T7 Endonuclease I-based Mutation Detection with the EnGen® Mutation Detection Kit (NEB #E3321) to detect how efficiently a given sgRNA produces indels at targeted genomic DNA locus ...
-
bioRxiv - Cell Biology 2024Quote: ... rRNA-depleted RNA was used to generate the RNA-sequencing libraries using NEB Ultra II directional kit (New England Biolabs). Samples from Keratinocytes were multiplexed and sequenced on a HiSeq4000 PE 150 sequencing platform (Illumina) ...
-
bioRxiv - Molecular Biology 2024Quote: ... The 3′ adapter was conjugated with an amino CA linker instead of dCC at the 3′ end (GeneDesign) and adenylated using a 5′ DNA adenylation kit at the 5′ end (NEB). To reduce ligation bias ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... genomic DNA was extracted from each homogenized pool of 100 flies using the Monarch Genomic DNA Purification Kit (New England Biolabs). DNA quality was assessed using Nanodrop and quantified with Qubit ...
-
bioRxiv - Microbiology 2024Quote: ... equal volumes of cDNA were used for qPCR using the Luna® Universal qPCR Master Mix kit (New England Biolabs). For qPCR ...
-
bioRxiv - Physiology 2024Quote: ... The libraries for the Illumina sequencer (paired-end 150 bp) were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... RNA samples were treated with DNAse I (New England Biosciences) and were concentrated through a Monarch RNA Cleanup Kit (NEB) to ensure no carryover of contaminants.
-
bioRxiv - Bioengineering 2024Quote: Libraries for RNA sequencing were generated using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #7765L) in conjunction with NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors RNA Set1 ...
-
bioRxiv - Biophysics 2024Quote: ... The codon optimized full-length LRRK2 sequence was inserted into the same vector used previously via Gibson assembly and LRRK2 mutant as cloned using the Q5 Site-Directed Mutagenesis Kit (NEB) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... Library preparation was carried out by the Edinburgh Clinical Research Facility from 500 ng of each RNA sample using the NEBNext Ultra II Directional RNA library kit with PolyA enrichment module (New England Biolabs). Libraries were assessed for quality and fragment size using the Agilent Bioanalyser ...
-
bioRxiv - Cell Biology 2024Quote: Site directed mutagenesis was performed using the Q5® Site-Directed Mutagenesis Kit according to the manufacturer’s instruction (New England BioLabs Inc. (NEB), Ipswich ...
-
bioRxiv - Cancer Biology 2024Quote: ... mRNA magnetic isolation module and sequencing libraries were created using NEBNext Ultra II direction RNA library prep kit for Illumina (New England Biolabs). DNA libraries were validated with TapeStation (Aligent Technologies ...
-
bioRxiv - Cancer Biology 2024Quote: ... C1- and HMG-domain deletion and point-mutant constructs were cloned from pcDNA3.1-HA-CIC::DUX4 (a gift from Takuro Nakamura (6)) using the Q5 Site-Directed Mutagenesis Kit (NEB E0554S) with the primers described in Supplemental Dataset S2 ...
-
bioRxiv - Genetics 2024Quote: ... Cas9H840A was made by site-directed mutagenesis of pLS504 using the Q5 site-directed mutagenesis kit (New England Biolabs #E0554S) using primers MK118 and MK119 to yield pLS517 ...
-
bioRxiv - Cell Biology 2024Quote: ... The vector backbone was also PCR-amplified and the three fragments were assembled with NEBuilder HiFi DNA assembly kit (New England Biolabs) according to manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2024Quote: ... 5 ng of DNA for each sample was then used with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) and completed according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2024Quote: ... range 9.0-9.5] were converted into sequencing libraries with the “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” (#E7760, New England Biolabs) following the manufacturer’s instructions ...
-
bioRxiv - Physiology 2024Quote: ... Five hundred micrograms of total RNA was used to prepare strand-specific libraries by the NEBNext Ultra II Directional RNA Library Preparation kit (NEB) and sequenced (75 nucleotides ...
-
bioRxiv - Genomics 2024Quote: ... PCR amplified and then pooled into equimolar amounts and further quantified using the NEBNext Library Quant Kit (New England Biolabs). The pooled library was subjected to 50 bp paired-end sequencing on the Illumina NextSeq 2000 platform.
-
bioRxiv - Microbiology 2024Quote: ... Libraries were prepared using NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (New England Biolabs; #E6177) per manufacturers protocol with 50-100 ng of DNA input and 5-6 cycles of PCR ...
-
bioRxiv - Immunology 2024Quote: ... Stranded RNA sequencing libraries were prepared as described using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (Cat# E7760L; New England Biolabs). Purified libraries were qualified on an 4150 TapeStation (Agilent Technologies ...
-
bioRxiv - Cell Biology 2024Quote: ... Total RNA obtained from each sample was subjected to sequencing library construction using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) with NEBNext Poly(A ...
-
bioRxiv - Microbiology 2024Quote: ... and processed into a cDNA library for Illumina with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Dual-index primers were conjugated with NEBNext Multiplex Oligos for Illumina (Set1 ...
-
bioRxiv - Cell Biology 2024Quote: ... To generate pcDNA5/FRT-Tg-NLuc plasmids the Tg gene was amplified from the pcDNA5/FRT-Tg-FLAG plasmid and assembled with the NLuc fragment using a HiFi DNA assembly kit (New England BioLabs). To generate the respective mutant construct plasmids for A2234D and C1264R Tg ...
-
bioRxiv - Molecular Biology 2024Quote: ... On-column DNase I digestion was performed followed by mRNA isolation with the NEBNext Poly (A) mRNA Magnetic Isolation Kit (NEB). Library preparation was done with the Ultra II Directional RNA Library Prep for Illumina following the manufacturer’s manual ...
-
bioRxiv - Biochemistry 2024Quote: ... Site-directed mutagenesis was performed using standard PCR-based mutagenic procedures with the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) following the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2024Quote: ... insert and vector bands were separated by agarose gel electrophoresis and purified with the Monarch DNA Gel Extraction Kit obtained from New England Biolabs (NEB). Ligations were done with the Quick LigationTM Kit (NEB) ...
-
bioRxiv - Cancer Biology 2024Quote: ... A sequencing library was generated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, 7645). ChIP-seq was carried out on NovaSeq 6000 (Illumina ...
-
bioRxiv - Cancer Biology 2024Quote: mRNA was isolated from 6-well plates showing a cell confluency of ∼90% using the Monarch Total RNA Miniprep kit (NEB). siRNA transfection was performed 48 hours prior to RNA isolation using the Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific ...