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Citations for New England Biolabs :
651 - 700 of 3027 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... Linear transformation cassettes were excised from plasmids with EcoRV (New England Biolabs) prior to transformation.
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bioRxiv - Microbiology 2021Quote: ... Restriction enzymes were purchased from New England Bio-labs (NEB, MA, USA). Oligonucleotides (sequences provided in Table S2 ...
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bioRxiv - Microbiology 2020Quote: ... PCRs were performed with the Phusion High-Fidelity DNA polymerase from NEB, using the Phusion GC Buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... SNAPf_6xHis was amplified from this construct by PCR using VENT Polymerase (NEB), forward primer 5’-AAAAGAATTCAACCATGGACAAAGACTGCGAAA-3’ and reverse primer 5’-TTTTGAATTCATTAGTGATGGTGATGGTGATGGGA-3’ (EcoR1 site are underlined) ...
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bioRxiv - Neuroscience 2022Quote: ... The guide RNA and Cas9 (EnGen Cas9 NLS from New England Biolabs) were transfected by using the NEON transfection system (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... Scaffold strands (p8064, produced as previously described41 and phiX174, purchased from NEB) were used at 10 nM ...
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bioRxiv - Biochemistry 2022Quote: ... The PCR product was inserted into the linearised (SalI, XbaI from NEB) pRS1024 yeast expression vector (Kellosalo et al. ...
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bioRxiv - Bioengineering 2022Quote: ... 0.5 of Murine RNase Inhibitor (purchased from New England Biolabs, cat# M0314S), 2 μL of FQ-labeled reporter (RNaseAlert substrate purchased from IDT ...
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bioRxiv - Microbiology 2022Quote: ... Restriction enzymes were purchased from New England Biolabs (NEB, Ipswich, MA, USA). DNA ligase ...
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bioRxiv - Synthetic Biology 2022Quote: ... used enzymes and buffers from New England Biolabs (NEB; Ipswich, MA, USA) according to the relevant NEB protocols ...
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bioRxiv - Synthetic Biology 2022Quote: ... All enzymes for plasmid construction were obtained from New England Biolabs (NEB). Constructs were chemically transformed into E ...
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bioRxiv - Synthetic Biology 2022Quote: ... PURExpress and all other enzymes were purchased from New England Biolabs (NEB). E ...
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bioRxiv - Neuroscience 2022Quote: ... The fragments were ligated using T4 DNA ligase (all enzymes from NEB).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Phusion® High-Fidelity DNA Polymerase was from NEB (Beverly, MA, USA). EDTA-free protease inhibitor was purchased from Roche (Basel ...
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bioRxiv - Molecular Biology 2022Quote: ... Additional enzymes were from commercial sources: E.coli Topoisomerase I (TopA) (NEB #M0301S); Nuclease P1 (NEB #M0660S) ...
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bioRxiv - Genomics 2022Quote: ... The products were eluted from the NEBNext polyA magnetic isolation (NEB, E7490S) in tris buffer ...
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bioRxiv - Genomics 2022Quote: ... 1X NEB Buffer 2 and 0.20μg/μl BSA (all from NEB, USA) was added to the wells and incubated at 25°C for 1.5h ...
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bioRxiv - Molecular Biology 2022Quote: ... CaMKAR was isolated from soluble lysate by nickel chromatography NEBExpress columns (NEB). Soluble protein was quantified by Pierce BCA assay (ThermoFisher ...
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bioRxiv - Biophysics 2024Quote: ... SNAP-Surface Alexa Fluor 647 (catalog no. S9136S) was obtained from NEB. 40 nm and 90 nm diameter gold nanoparticles (catalog no ...
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bioRxiv - Synthetic Biology 2024Quote: All enzymes were purchased from New England Biolabs (NEB, Ipswich, MA, USA) and ThermoFisher Scientific (Waltham ...
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bioRxiv - Molecular Biology 2023Quote: ... ii) constructed using Gibson Assembly (GA) protocol from New England Biolabs (NEB) or iii ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were isolated from selected transformants using Monarch plasmid miniprep kit (NEB) and subjected to sequencing reactions (Eurofins ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted from samples using Monarch RNA Cleanup Kit (NEB T2030L) and quantified using a Nanodrop 2000 Spectrophotometer 2000.
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bioRxiv - Synthetic Biology 2023Quote: ... and other molecular biology reagents were purchased from New England Biolabs (NEB). Plasmid extraction mini kit and Gel/PCR mini kit were purchased from Favogen Biotech Corporations.
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bioRxiv - Biochemistry 2023Quote: ... 75 mM NaCl) from clarified lysates using Chitin Resin (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... and ligases were purchased from New England Biolabs (NEB; Ipswich, MA, USA). Sanger DNA sequencing and oligonucleotide synthesis were performed by Microsynth (Balgach ...
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bioRxiv - Developmental Biology 2023Quote: ... from template DNA plasmid digested by NotI or SpeI (New England Biolabs), and were cleaned by RNeasy mini kit (QIAGEN) ...
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bioRxiv - Cancer Biology 2023Quote: RNA was harvested from 1 million cells using Bio-TRI® (BioLabs) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was harvested from the treated cells using Bio-TRI® (BioLabs) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... by Zymo Research and incubated with the second strand synthesis kit from NEB (E6111S). Double-stranded DNA was then entered into the end-repair module of RNA Library Prep Kit for Illumina from NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... nucleic acids extracted from SPARDA were phosphorylated by T4 polynucleotyide kinase (NEB) according to the manufacturer’s protocol and were separated by 18% PAGE ...
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bioRxiv - Microbiology 2023Quote: Cloning was performed according to standard procedures using enzymes purchased from NEB, unless stated otherwise ...
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bioRxiv - Microbiology 2023Quote: ... RNA was isolated from using the Monarch Total RNA miniprep kit (NEB), using the Tough-to-lyse sample protocol with bead-beating (Tissuelyser LT ...
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bioRxiv - Biophysics 2023Quote: ... Purple ‘no SDS’ loading dye was purchased from New England Biolabs (NEB). PEG:bioPEG microscopy chips were purchased from Oxford Nanoimaging (Oxford ...
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bioRxiv - Microbiology 2023Quote: Restriction modification was performed using enzymes purchased from NEB (New England Biolabs) according to manufacturer’s specifications ...
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bioRxiv - Cell Biology 2024Quote: DNA from lysed worms was amplified using OneTaq polymerase (New England BioLabs) and the primers listed in Table S2 that were designed based on sequences in WormBase (17) ...
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bioRxiv - Biochemistry 2024Quote: ... and NEBNext® Multiplex Oligos for Illumina® were purchased from NEB and stored at −20°C.
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bioRxiv - Microbiology 2024Quote: ... The qPCR was performed with Luna Universal qPCR Master Mix from NEB and primer sets for gyrB (gyrB_Fwd and gyrB_Rev) ...
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Synergistic amplifier reprograms biosensor behavior to facilitate detection of dioxin-like compoundsbioRxiv - Molecular Biology 2024Quote: ... and T4 DNA ligase were purchased from New England Biolabs (NEB, USA). E ...
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Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... Deglycosylation was performed with either PNGase F or EndoH (both from NEB), following the protocol of the manufacturer.
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bioRxiv - Microbiology 2024Quote: ... and HiFi DNA Assembly kit were purchased from New England Biolabs (NEB). All bacterial strains were PCR or sequence verified before use ...
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bioRxiv - Microbiology 2024Quote: ... gRNA oligos from IDT were cloned into Pss013 using Gibson Assembly (NEB). Plasmids were transformed into DH5α E ...
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bioRxiv - Microbiology 2024Quote: ... MBP fusion proteins were detected with a rabbit anti-MBP from NEB company 1:1000 followed by an incubation with a goat anti-rabbit HRP-conjugated (1:3000) ...
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bioRxiv - Plant Biology 2024Quote: ... construct-specific endonucleases and T4 DNA ligase (all enzymes from NEB BioLabs), according to the manufacturer’s directions ...
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bioRxiv - Plant Biology 2024Quote: ... construct-specific endonucleases and T4 DNA ligase (all enzymes from NEB BioLabs), according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2024Quote: ... The cloning method was followed according to a standard method from NEB 71 ...
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bioRxiv - Immunology 2024Quote: ... The mCherry ORF was excised from pGW163 using NcoI and HindIII (NEB) and subsequently replaced with the tailed rpoS DNA fragment using the HiFi DNA assembly kit (NEB) ...
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bioRxiv - Synthetic Biology 2024Quote: ... DeepVent and DeepVent (exo-) polymerases and PCR buffers were purchased from NEB. SD Polymerase was purchased from Bioron ...
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bioRxiv - Biophysics 2024Quote: ... We used a pUC19 vector (2,686 bp) from New England Biolabs (NEB) because it has single BsaI restriction endonuclease site ...
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bioRxiv - Biochemistry 2024Quote: ... 75⍰mM NaCl) from clarified lysates using Chitin Resin (New England Biolabs) following the manufacturer’s protocol ...