Labshake search
Citations for New England Biolabs :
8851 - 8900 of 10000+ citations for Mouse Dachshund Homolog 1 DACH1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Each of the 11 genes were cloned into a pENTR-DTOPO vector and finally into a pIX-HALO expression vector using a Gateway cloning kit obtained from NEB and following the manufacturer’s protocol (Supplemental Datasets S9-S19) ...
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bioRxiv - Molecular Biology 2023Quote: ... and size selected by running samples on a 1.8% LMT agarose gel at 120 V for 25 minutes at 4°C and gel extracting the mononucleosome band with a Monarch Gel Extraction Kit (T1020S, New England Biolabs). Purified samples were carried forward for qPCR or library preparation ...
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bioRxiv - Cell Biology 2023Quote: ... double and triple N>G mutations were introduced in the parental constructs by using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) according to the manufacturers protocols and using custom designed primer ...
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bioRxiv - Microbiology 2023Quote: ... and given barcode sequences using the NEBnext Ultra-directional RNA Library Kit for Illumina (New England Biolabs, Ipswitch, MA, USA) with NEBnext Multiplex Oligos for Illumina (Primer Set 1 ...
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bioRxiv - Microbiology 2022Quote: ... Illumina sequencing libraries for each genome were constructed using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) using the manufacturer’s protocol with DNA input concentrations of 5-15 ng/µL ...
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bioRxiv - Microbiology 2023Quote: ... Cells were centrifuged 7,000xg/2min pellet was washed in 1ml of PBS and then resuspended in 400 μl of the resuspension buffer (Monarch Miniprep kit – NEB) containing 50 μg of lysostaphin ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing library was generated using NEB Next® Ultra TM DNA Library Prep Kit for Illumina (NEB, USA, Catalog#: E7370L) following manufacturer’s recommendations and index codes were added to each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... The remaining steps of library preparation were done using and the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). Adapters and PCR primers were purchased from New England BioLabs.
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bioRxiv - Molecular Biology 2023Quote: ... Nucleotide substitution or deletion mutations were designed on the NEBaseChanger online tool and TOPO-cloned using the Q5 site-directed mutagenesis kit (New England Biolabs) according to the standard protocol.
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bioRxiv - Molecular Biology 2023Quote: ... . Site-directed mutagenesis was performed on 10 ng pET28a(+)-HisPI31wt template with the Q5 Site-Directed Mutagenesis Kit (New England BioLabs) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... The final cleaned-up Hi-C library was used as input material for Illumina sequencing library prep kit (NEB, E7805) with 6-8 cycles of PCR amplification using KAPA-HiFi (Kapa Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... These DNA constructs were used as a template to in vitro transcribed RNA using T7 high yield RNA kit (New England Biolabs). The synthesized RNA was DNase treated (TURBODNase) ...
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bioRxiv - Biophysics 2023Quote: ... DNA fragments were generated by PCR amplification and then fused with the backbones using NEBuilder HiFi DNA assembly kit (New England Biolabs). Constructs were transformed and amplified in NEB 5-alpha Competent E ...
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bioRxiv - Biochemistry 2023Quote: ... the pool was subjected to NEBNext FFPE DNA Repair and Ultra II End-prep kits (M6630 and E7546, New England Biolabs). After that ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were constructed using approximately 6 ng of total EV RNA as input material for the RNA sample preparations using the Small RNA Library Prep Set for Illumina kit (New England BioLabs) following manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... where the band shift due to the insertion of the BSR cassette was confirmed in the case of homologous recombination and purified using a PCR purification kit (NEB), in which the nucleotide sequence was determined (Genewiz) ...
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Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: ChIP-seq Illumina libraries were prepared for immunoprecipitated and input samples using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB). Following adapter ligation ...
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bioRxiv - Plant Biology 2023Quote: ChIP-seq libraries were constructed from 100 ng of DNA samples using the NEB Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... which were in vitro transcribed with the mRNA products being capped and polyadenylated by using HiScribe T7 ARCA mRNA Kit (New England Biolabs). mRNA products were column-purified (New England Biolabs ...
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bioRxiv - Neuroscience 2023Quote: Quantitative real-time PCR (qRT-PCR) was used to measure RNA with Luna Universal One-Step RT-qPCR Kit (New England Biolabs) via CFX Connect Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Pathology 2023Quote: ... cDNA libraries for sorted KCs were generated using Next Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). RNA-Seq libraries were sequenced on an Illumina NovaSeq 6000 (40 million reads per sample) ...
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bioRxiv - Plant Biology 2023Quote: ... The mutant versions of MUTE constructs were generated in the same way after the site-directed mutagenesis using Q5 Site-directed mutagenesis kit (NEB) and subjected to the Three-Way Gateway reaction after sequence confirmation ...
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bioRxiv - Zoology 2023Quote: ... followed by the production of stranded cDNA libraries with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Individual barcodes were applied with NEBNext dual adaptors (New England Biolabs ...
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bioRxiv - Neuroscience 2023Quote: RNA was released from the beads through protein K lysis (Monarch® Total RNA Miniprep Kit; New England Biolabs, #T2010S). After lysis ...
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bioRxiv - Cell Biology 2023Quote: ... Libraries for RNA-Seq were prepared using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced on the NextSeq500 (Illumina ...
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bioRxiv - Physiology 2023Quote: ... purified and analyzed to quantify 5-methylcytosine levels by applying the DNA Purification Kit (T3010S, Monarch, New England Biolabs, USA) and the 5mC Assay Kit (lot no ...
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bioRxiv - Plant Biology 2023Quote: Site direct mutagenesis of GPS1 (plasmids pDR-FLIP43 GPS1 and p16-FLIP43 nlsGPS1 (Rizza et al., 2017) was achieved using QuikChange site-directed mutagenesis kit and Phusion high-fidelity DNA polymerase (NEB) following manufacturer protocol resulting in plasmids pDR-FLIP43 GPS2 (for yeast expression ...
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bioRxiv - Cell Biology 2023Quote: ... DNA libraries were produced using NEBNext Ultra II DNA Library Preparation kit and NEBNext Unique Dual Index Primers (New England Biolabs). Libraries were quality checked using Agilent D1000 TapeStation kit and Qubit Flourometer and then sequenced on the Illumina NovaSeq X (Admera Health ...
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bioRxiv - Systems Biology 2024Quote: Approximately 100 ng of each RNA sample was used to create Illumina sequencing libraries using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... Approximately 100 ng of each DNA sample was used to create Illumina sequencing libraries using a NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs (NEB), E7805S) ...
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bioRxiv - Systems Biology 2024Quote: 50 ng RNA of each sample were reverse transcribed and barcoded by using the PCR-cDNA barcoding kit (SQK-PCB111.24) and NEBNext Companion Module (NEB E7180L). Libraries were then sequenced on the Nanopore PromethION platform.
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bioRxiv - Plant Biology 2024Quote: Whole Methylome Sequencing (WMS) was performed on genomic libraries prepared using the NEBNext Enzymatic Methyl-seq Kit (New England BioLabs) following the manufacturer instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Sequence fragments of ATML1 (START1, START2, and didomain) were generated using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) with the following primers ...
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bioRxiv - Cancer Biology 2024Quote: The purified DNA was fragmented using the Covaris M220 and the libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) for next generation sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... The peptides obtained by trypsin digestion were subsequently labeled as directed by the tandem mass tag (TMT) kit (PTM Biolabs). The labeling peptides were then fractionated by high pH reverse-phase high-performance liquid chromatography and further subjected to liquid chromatography with tandem mass spectrometry (LC-MS/MS ...
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bioRxiv - Neuroscience 2024Quote: ... using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Cell Biology 2024Quote: ... The remaining steps of library preparation were done using and the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). Adapters and PCR primers were purchased from New England BioLabs ...
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bioRxiv - Molecular Biology 2023Quote: ... Resulting footprints were then used to generate sequencing libraries using the NEBNext Small RNA Library Prep Set for Illumina kit (New England BioLabs). Library quality was assessed using High Sensitivity DNA Chips with the Agilent 2100 Bioanalyzer System (Agilent Technologies).
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bioRxiv - Biochemistry 2024Quote: ... reverse 5’-GTGGCC CTCGAG TCA GTG AGT TTC ATG TTG G-3’ and then purified using the Monarch PCR plus DNA purification kit (NEB). The purified PCR product was digested by KpnI and XhoI (NEB ...
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bioRxiv - Microbiology 2023Quote: ... individual barcodes were incorporated into the dA-tailed DNA using the EXP-NBD104 and EXP-NBD114 native barcoding expansion kit following the ONT protocol with NEB Blunt//TA Ligase Master Mix (New Englands Biolabs). Barcoded DNA samples were then equimolarly pooled ...
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bioRxiv - Microbiology 2023Quote: ... Purified DNA was then constructed into libraries suitable for Illumina sequencing using the NEXT Ultra II library preparation kit (NEB). ChIP libraries were sequenced on the Illumina Hiseq 2500 or Nextseq 550 at the Tufts University Genomics facility.
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bioRxiv - Molecular Biology 2023Quote: ... 500ng of total RNA was used for reverse transcription using ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs). For quantification ...
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bioRxiv - Microbiology 2023Quote: ... RNA sequencing libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs) and the DNA library was prepared using the NEBNext® Microbiome DNA Enrichment Kit (New England Biolabs) ...
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bioRxiv - Microbiology 2023Quote: ... and the appropriate crRNA gBlock (IDTDNA; Coralville, IA) was inserted using the NEBuilder HiFi DNA assembly kit (New England Biolabs (NEB); Cambridge ...
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bioRxiv - Microbiology 2023Quote: ... whereas PCR fragments from the agarose gel were purified using the High-Yield PCR Cleanup and Gel Extraction Kit (New England BioLabs). Site-specific mutagenesis of efp was carried out with the Q5® Site-Directed Mutagenesis Kit (New England BioLabs ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions using equimolar amount of DNA were carried out in presence or absence of 50 nM CTCF using a HiScribe T7 High Yield RNA Synthesis Kit (NEB), adding 100 μM Cy3‐UTP ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 bp Cy3‐UTP labeled RNA was generated using a PCR‐product containing a T7‐promoter site and the HighScribe T7 high yield RNA synthesis kit (NEB) as well as Cy3‐UTP (Jena Bioscience) ...
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bioRxiv - Molecular Biology 2023Quote: Sequencing libraries of DNA fragments isolated following CUT&RUN were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) and the NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Microbiology 2023Quote: Genes of interest were PCR-amplified with 25 bp homology arms from high-titer phage lysates and ligated into respective plasmid backbones using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). Recombinant plasmids were transformed into E ...
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bioRxiv - Immunology 2023Quote: RNA of the carriers and non-carriers were constructed into transcriptome libraries using NEBNext Single Cell/Low Input RNA Library Prep Kit (New England BioLabs) and sequenced using NextSeq2000 (Illumina ...