Labshake search
Citations for New England Biolabs :
8751 - 8800 of 9360 citations for Rat CD325 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... Library preparation was performed using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs Inc., Ipswich, MA, USA). The quantity of the prepared DNA libraries was determined using a Qubit© 2.0 Fluorometer (Thermo Fisher Scientific Inc. ...
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bioRxiv - Genomics 2023Quote: ... 1.2 μg of RNA was used as the starting material for the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA). Poly-A RNA was purified as per instructions and converted to adapter-ligated ...
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bioRxiv - Pathology 2023Quote: ... The sample preparation was performed according to the protocol “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” (NEB #E7760S/L). Briefly ...
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bioRxiv - Plant Biology 2023Quote: ... The YFP-CESA6 construct was used as a template to prepare 18 YFP-CESA6 mutated constructs using a Q5 Site Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA) with the specific primers listed in Supplemental Table S4 ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA-seq libraries were prepared from total RNA using the Ultra II RNA Sample Prep Kit (New England BioLabs, Ipswitch, MA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Wild-type or mutant plasmid was linearized using restriction digestion and then subsequently used for in vitro transcription (HiScribe® T7 High Yield Synthesis Kit, NEB E2040) to synthesize wild-type and mutant N gene RNA ...
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bioRxiv - Bioengineering 2022Quote: ... These linearized templates were used to direct in vitro transcription (IVT) of mRNAs using the HiScribe™T7 mRNA Kit with CleanCap® Reagent AG (NEB). Cy3-UTP and Cy5-UTP were used replacing 25% of standard UTP in some experiments to fluorescently label the mRNA transcripts ...
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bioRxiv - Cell Biology 2023Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Genomics 2022Quote: ... and then used for qPCR or converted into sequencing libraries following the NEBNext® Ultra™ II DNA Library Prep Kit (NEB). For ChIP-qPCR ...
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bioRxiv - Genomics 2022Quote: An Illumina sequencing DNA library was prepared from the same DNA sample using the NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (New England Biolabs). An Omni-C library was prepared for a separate visceral sample using the Dovetail™ Omni-C™ Kit with Library Module and Primer Set for Illumina (Dovetail Genomics ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The beads were washed and re-suspended in elution buffer and used for library preparation using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB). The amplified final libraries were sequenced on the Illumina HiSeq X Ten platform (San Diego ...
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bioRxiv - Genetics 2023Quote: ... RNA-seq libraries were prepared using the NEBNext UltraII Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, USA). Sequencing was performed using HiSeq X Ten (Illumina) ...
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bioRxiv - Evolutionary Biology 2023Quote: Sequencing libraries were prepared from 0.5 μg total RNA using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... 1 µg of the duplex was in vitro transcribed using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB # E2050S) following the standard IVT protocol and purified using Monarch® RNA Cleanup columns (NEB # T2030).
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bioRxiv - Plant Biology 2023Quote: ... pCAMBIA0380 was digested by EcoRI and XhoI and used for the cloning of three PCR fragments with Gibson Assembly cloning kit (NEB, Evry, France) according to the supplier’s indications ...
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bioRxiv - Biochemistry 2023Quote: ... ABCB5β with ABCB6 and ABCB5β with ABCB9 coding sequences were fused following the NEBuilder HIFI DNA Assembly kit’s instructions (New England Biolabs, Ipswich, MA, USA). The ABCB5β-ABCB6 ...
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bioRxiv - Microbiology 2023Quote: ... The final pool was run on an Agilent Bioanalyzer dsDNA High Sensitivity chip and quantified using NEBNext Library Quant Kit for Illumina (cat # E7630L, New England Biolabs, Ipswich, USA).
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bioRxiv - Genomics 2023Quote: The 40 libraries were constructed with NEBNext® Ultra II DNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA) according to the instruction ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we purified the products of the ace-1 PCR (see above) using the BS664-250 Preps EZ-10 Spin Column PCR Purification kit (New England BioLabs, Evry France). The purified PCR products were then cloned (TOPO TA Cloning Kit pCR 2.1-TOPO Vector and TOP10F’ invitrogen bacteria) ...
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bioRxiv - Genomics 2023Quote: ... Illumina libraries for all 4 isolates were constructed using the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs Inc., USA) as per standard protocol and sequenced using the Illumina MiSeq and NextSeq500 platforms (paired end ...
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bioRxiv - Bioengineering 2023Quote: ... was amplified by PCR from the same vector and its DNA was extracted using the Monarch DNA Gel Extraction Kit (New England Biolabs, Ipswich, MA). GLuc was inserted into the digested backbone through Gibson Assembly Hi-Fi kit (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... 3μg RNAs of each biological sample were collected to construct sequencing library using NEB Next Ultra Directional RNA LibraryPrep Kit for Illumina (NEB, Ispawich, USA). The mRNAs were measured by DEGseq software to find differential expressed genes (DEG ...
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bioRxiv - Cell Biology 2023Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA libraries were prepared from 50 ng (hippocampi) or 10 ng (amygdalae) RNA using the NEBNext® Ultra™ II RNA Library Prep Kits for Illumina (New England Biolabs). Libraries were sequenced (40M bp sequencing depth ...
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bioRxiv - Biochemistry 2023Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II directional RNA library prep kit with the NEBNext Poly(A) mRNA magnetic isolation module (New England Biolabs, E7490L, E7760L) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... ChIP and input libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswitch, MA, USA). Libraries were sequenced on an Illumina NextSeq500 (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... and library preparation was performed with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs, catalog # E6420) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and Illumina-compatible libraries prepared using the NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs E7760S). Libraries were sequenced on the Illumina HiSeq3000 system ...
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bioRxiv - Cell Biology 2023Quote: RNA was extracted from HCT116 Ki-67-AID in presence or absence of Ki-67 and extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs, Hitchin, UK) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Quality control of libraries was performed using a Bioanalyzer and the final NGS libraries were quantified using NEBNext Library Quant Kit for Illumina (New England Biolabs, Cat. E7630L). For NGS ...
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bioRxiv - Immunology 2023Quote: ... Quality control of libraries was performed using an Agilent 2100 Bioanalyzer and the final NGS libraries were quantified using NEBNext Library Quant Kit for Illumina (New England Biolabs, Cat. E7630L). For NGS ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared with the NEBNext Ultra II Directional RNA library prep kit for Illumina (New England Biolabs, Ipswich, Massachusetts, USA) and checked by chip-based ...
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bioRxiv - Molecular Biology 2023Quote: cDNA libraries were prepared from 500 ng RNA using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs; Ipswich, MA) with rRNA depletion ...
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bioRxiv - Microbiology 2023Quote: ... The sequencing library employed for total RNA sequencing was prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Cat# E7530). Paired-end 76-bp sequencing was performed using a MiSeq system (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were prepared using NEBNext Ultra II Directional RNA library preparation kit for Illumina (New England Biolabs GmbH, Frankfurt am Main, Germany). Libraries were sequenced to the depth of at least 60 million reads ...
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bioRxiv - Systems Biology 2023Quote: ... Base editor mRNAs were generated by in vitro transcription using the HiScribe T7 High-Yield RNA synthesis kit (NEB Cat No. E2040S) via the method described previously86 ...
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bioRxiv - Cancer Biology 2023Quote: Purified DNA was used to generate libraries using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs cat# 7370) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... This plasmid was used as a template for G2019S and I2020T site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, NEB, Catalog # E0445S). These plasmids were used for the generation of recombinant baculoviruses following Bac-to-Bac expression system protocols (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Genomics 2023Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following the manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... viral RNA was produced via in vitro transcription of an XbaI-linearized HCV-Jc1 or AflII-linearized YFV-17D plasmid using the HiScribe® T7 High Yield RNA Synthesis Kit (HCV, New England Biolabs E2040S) or mMESSAGE mMACHINE™ SP6 Transcription Kit (YFV-17D ...
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bioRxiv - Cancer Biology 2023Quote: ... 800 ng of RIN ≥7 of total RNA was used for library construction using a NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... DNA was sheared to fragments of ∼300 bp and libraries were created using the NEBNext Ultra DNA Library Prep Kit (New England Biolabs, Ipswich, MA). An Illumina NextSeq 500 sequencer (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... The sequencing library employed for total RNA sequencing was prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Cat# E7530). Paired-end 76-bp sequencing was performed using a MiSeq system (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... RNA sequencing library preparation uses NEB Next Ultra II RNA Library Preparation Kit for Illumina by following the manufacturer’s recommendations (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was isolated by acid guanidinium thiocyanate-phenol-chloroform-based extraction and purified from the aqueous phase using the Monarch RNA Clean Up Kit (NEB, Frankfurt, Germany). RNA quality was assessed by agarose gel electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: ... total RNA libraries were built using the NEBNext Ultra II library prep kit for Illumina (New England BioLabs inc., product code: E7775) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The optimized for expression in PAO1 cell gene gp180 was synthesized by IDT and inserted into pHERDmCh using NEBuilder® HiFi DNA Assembly kit (New England Biolabs, UK).
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bioRxiv - Molecular Biology 2023Quote: ... SCAR-seq libraries were prepared following the previous published protocol (11) without any modification except using NEBNext® Ultra™ II DNA library prep kit (NEB) for the end repair ...
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bioRxiv - Biochemistry 2023Quote: ... respective FoxP3 CDS with overhangs of pcDNA vector was synthesized by IDTDNA and then assembled with NEBuilder® HiFi DNA Assembly Cloning Kit (NEB, #5520G). FoxP3 paralogs (FoxP1 ...