Labshake search
Citations for New England Biolabs :
8651 - 8700 of 9360 citations for Rat CD325 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The sequencing library for total RNA-sequencing was prepared using NEB Next Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Cat# E7530). Paired-end ...
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bioRxiv - Cancer Biology 2022Quote: ... We designed spCas9 guide RNAs to target codon 12 of the human KRAS gene and selected the one with highest targeting efficiency determined by T7E1 mismatch assay (EnGen Mutation Detection Kit, New England Biolabs, Ipswich, MA). Truncated guide RNA was produced by PCR assembly of a guide RNA template (56 ...
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bioRxiv - Microbiology 2024Quote: Complimentary DNA (cDNA) was prepared from 250 ng of total RNA using the First Strand cDNA Synthesis Kit (New England BioLabs Inc., USA), according to the manufacturer’s protocol ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... RNA sequencing libraries were prepared using the NEB-Next Ultra RNA Library Prep Kit for Illumina using manufacturer’s instructions (NEB, Ipswich, MA, USA). The samples were sequenced on the Illumina 4000 instrument ...
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bioRxiv - Molecular Biology 2024Quote: ... Enriched DNA was subjected to library construction using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (catalog no. E7645L, NEB), with the adapter ligation step performed at 50°C instead of 65°C ...
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bioRxiv - Cell Biology 2024Quote: ... RNA sequencing library preparation used the NEBNext Ultra RNA Library Prep Kit for Illumina followed by manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1pmol of each fragment were then assembled for 1h at 50°C using NEBuilder HiFi DNA Assembly master mix from the NEBuilder HiFi DNA Assembly Cloning Kit (NEB, ref E5520S). Reaction products were amplified in provided bacteria and purified using QIAprep Spin Miniprep Kit.
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bioRxiv - Immunology 2023Quote: ... The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA and library preparation were performed using NEBNext® Ultra™ II Directional RNA Library Prep kit for Illumina (New England Biolabs). Library concentration was determined using NEBNext® Library Quant Kit for Illumina (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... Two hundred nanograms of RNA was used to generate sequencing libraries using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® Kit (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was prepared with 1 µg of total RNA using the Protoscript First Strand cDNA Synthesis kit (New England Biolabs; Catalog #E6560L) as instructed by the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ... at 37° C for 2 hrs from 1 µg purified dsDNAs using a T7 high yield RNA synthesis kit (NEB, Cat # E2040S) in a total volume of 20 µL ...
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bioRxiv - Microbiology 2024Quote: ... 3 ug RNA was treated with Promega RQ1 DNase (M6101) then cleaned up with the Monarch® RNA Cleanup Kit (NEB Biolabs, T2050S) and eluted in 30 µl dH2O.
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bioRxiv - Systems Biology 2024Quote: ... 500 ng of total RNA was subjected to ribosomal-RNA depletion using the NEBNext rRNA Depletion kit (E7850L; New England Biolabs, Ipswich, MA), according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were prepared using a polyA selection method using the NEBNext Ultra II RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA) and quantified using Qubit 4.0 Fluorometer (Life Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... the sequencing libraries were generated using a NEBnext® Ultra™ II DNA library prep kit and NEBNext Multiplex Oligos (NEB #E7335) for Illumina (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... mCherry fluorescent protein gene was inserted into pHERD20T vector according to the manufacturing protocol of NEBuilder® HiFi DNA Assembly kit (New England Biolabs, UK). The resulting plasmid was referred to as pHERDmCh ...
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bioRxiv - Molecular Biology 2024Quote: ... EM-seq libraries were prepared with the NEBNext® Enzymatic Methyl-seq kit (E7120S) according to the manufacturer’s protocol (New England Biolabs, MA USA). Briefly ...
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bioRxiv - Immunology 2024Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA sequencing libraries were prepared using the NEB Next Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... 173 bp TBR-PCR target products were excised and purified using an Exo-CIP Rapid PCR Cleanup Kit (New England Biolabs, Ipswich, USA) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The libraries were then prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (New England Biolabs, MA, USA) in accordance with the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: Libraries were prepared using the NEBNext® DNA Library Prep Reagent Set for Illumina® kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were normalized to an input of 150 ng before preparation with a NEBNext® Ultra™ II RNA Library Prep (Magnetic Bead) kit (New England Biolabs). The polyA mRNA protocol was used for all preps ...
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bioRxiv - Microbiology 2024Quote: ... primers were designed to clone Ceg10 into pET28b using restriction enzymes NdeI and NotI by HiFi DNA Assemby kit (NEB; Table S4), producing the His6x-TEV-Ceg10 vector ...
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bioRxiv - Cell Biology 2024Quote: ... followed by stranded cDNA library generation using NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (#E7760L, New England Biolabs, US). All steps were performed as recommended in user manual E7760 (Version 1.0_02-2017 ...
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bioRxiv - Genomics 2023Quote: ... was extracted from hTERT RPE-1 dry cell pellets using the Monarch HMW DNA extraction kit for cells & blood (New England Biolabs, NEB T3050L) and for tissue (NEB T3060L) ...
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bioRxiv - Genetics 2024Quote: ... The B splice form (Spc105RB) was generated using site directed mutagenesis to delete the first intron from the A form (NEB BaseChanger Kit). All mutants were generated using site specific mutagenesis of the A- or B-form Spc105R coding region in the pENTR4 vector ...
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bioRxiv - Genomics 2024Quote: ... A library for short-read sequencing was prepared using an NEBNext Ultra II DNA Library Prep kit (New England Biolabs, Beverly, MA) and sequenced using a NovaSeq 6000 (Illumina ...
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bioRxiv - Developmental Biology 2024Quote: Sequencing libraries were prepared using a commercial kit (NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads, New England Biolabs, Ipswich, USA), and cDNA of three replicates per strain was sequenced as 150 bp (100 bp for Mpyr_CVe13f ...
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bioRxiv - Genomics 2024Quote: Sequencing libraries for regular cfDNA were prepared with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... RNA-Seq libraries were prepared using NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (Catalog #: E7760, New England Biolabs Inc.), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... high-molecular-weight (HMW) DNA was extracted from cultured lymphoblasts using the Monarch® HMW DNA Extraction Kit for Cells & Blood (New England Biolabs, T3050L). At all steps ...
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bioRxiv - Genomics 2024Quote: Libraries for metagenomic sequencing of CS2022-0121 and F16-31-UTM were prepared using a NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, Ipswich, MA) to elucidate the enrichment efficiency of the target capture sequencing ...
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bioRxiv - Microbiology 2024Quote: ... and assembled in frame into the digested pLIC-HA -DHFR-TS plasmid in frame with the 3x HA tag using the NEBuilder HiFi DNA Assembly cloning Kit (NEB, cat# E5520) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... was reverse transcribed from ∼1 μg high quality RNA using the ProtoScript® II First Strand cDNA Synthesis Kit (NEW ENGLAND BioLabs, Germany). Newly synthesized cDNA was purified using a the NucleoSpin® Gel and PCR Clean-up kit (Germany ...
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bioRxiv - Genomics 2024Quote: ... Double-stranded DNA libraries with 450 bp insert size were prepared using the ultraFS-II kit (New England Biolabs, London, United Kingdom). The QC check for the prepared libraries was done on an Agilent Tapestation (Santa Clara ...
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bioRxiv - Genomics 2024Quote: Base editor mRNAs were generated by in vitro transcription using the HiScribe T7 High-Yield RNA synthesis kit (NEB Cat No. E2040S) via the method described in48 ...
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bioRxiv - Microbiology 2024Quote: ... we used a similar protocol but library preparation was performed using the NEBNext Ultra DNA Library Prep Kit (New England BioLabs, ref. E7370) for Illumina following manufacturer’s specifications except for end repair ...
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bioRxiv - Biophysics 2023Quote: Purified linear and circular DNA were both in vitro transcribed using HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB, E2050S). 240 ng reaction was prepared according to the manufacturer’s recommendation (see details in Supplementary Methods) ...
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bioRxiv - Microbiology 2023Quote: ... Damaged double-stranded DNA in the initial DNA sample was repaired using the PreCR® Repair Mix Kit (New England Biolabs, USA) before library construction ...
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bioRxiv - Plant Biology 2023Quote: ... RNA libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs) and each individual library was barcoded using the NEBNext® Multiplex Oligos for Illumina® kit (New England Biolabs) ...
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bioRxiv - Biophysics 2023Quote: ... This plasmid was used as a template for G2019S and I2020T site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, NEB, Catalog # E0445S). These plasmids were used for the generation of recombinant baculoviruses following Bac-to-Bac expression system protocols (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... The sequencing library employed for total RNA sequencing was prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Cat# E7530). Paired-end 76-bp sequencing was performed using a MiSeq system (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... RNA sequencing library preparation uses NEBNext Ultra II RNA Library Preparation Kit for Illumina by following the manufacturer’s recommendations (NEB, Ipswich, MA, USA). Sequencing libraries were validated using the Agilent Tapestation 4200 (Agilent Technologies ...
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bioRxiv - Genetics 2023Quote: ... Ab aliquot of DNA (100 ng) was used to create libraries using the NEBNext Ultra II kit (New England Biolabs, Ipswich, Massachusetts). Libraries were sequenced at The University of Texas at Austin Genome Sequencing and Analysis Facility on a HiSeq 4000 ...
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bioRxiv - Genetics 2023Quote: ... We ran amplified DNA from wildtype and mutant individuals on an agarose gel and purified (Monarch DNA Extraction and Purification kit, new England Biolabs, MA, USA) and cloned the PCR products into JM109 competent cells using pGEM®-T Easy Vector Systems (Promega Corporation ...
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bioRxiv - Cell Biology 2023Quote: The 2NSP23 nanobody mRNA was prepared by in vitro translation of a DNA molecule of using the HiScribe™ T7 mRNA Kit with CleanCap® Reagent AG (New England BioLabs). The obtained mRNA has the following sequence and includes a 5’-CAP and a poly-A tail ...
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bioRxiv - Immunology 2023Quote: ... RNA sequencing library preparation uses NEBNext Ultra II RNA Library Preparation Kit for Illumina by following the manufacturer’s recommendations (NEB, Ipswich, MA, USA). Briefly ...