Labshake search
Citations for New England Biolabs :
8951 - 9000 of 9360 citations for Rat CD325 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Products were visualized on 2% agarose gels and successful amplification reactions were prepared for Sanger sequencing with Exo-CIP™ Rapid PCR Cleanup Kit according to the manufacturer’s protocol (New England Biolabs: E1050S, Ipswich, MA). Samples were then sequenced with amplification primers (Eurofins Genomics Services ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Libraries were prepared using NEBNext FFPE DNA Repair Mix in combination with the NEBNext Ultra II DNA Library Prep kits for Illumina (New England Biolabs, Ipswich, MA, USA) per manufacturer recommendations with custom inline barcodes as described in Moore et al ...
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bioRxiv - Cancer Biology 2022Quote: ... The libraries were prepared using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (New England BioLabs® Inc., USA). For sequencing ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and used to produce RNA-seq libraries with the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2019Quote: All plasmids were constructed through Gibson assembly (Gibson et al., 2009) using the NEBuilder HiFi DNA Assembly kit (New England Biolabs, Ipswich, MA, USA). Primers used in this study were purchased from Eurofins Genomics (Ebersberg ...
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bioRxiv - Genomics 2019Quote: ... Short-insert of 250 bp paired-end libraries were prepared for both individuals using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (Biolabs) and 10 PCR cycles ...
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bioRxiv - Cell Biology 2019Quote: ... and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina®(NEB, Ipswich MA). Libraries were pooled and single-end sequenced (1X75 ...
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bioRxiv - Microbiology 2019Quote: ... One hundred ng RNA from each sample were prepared for cDNA sequencing using NebNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... The reaction was topped up to 200μL with DEPC treated water and a phenol chloroform extract performed (as before except the pellet was resuspended in 17.5μL DEPC treated water. The SR RT primer (NEB small RNA kit for Illumina) was hybridized to the 3’ adapter and the 5’ adapter ligated by adding 2μL T4 RNA ligase buffer (NEB) ...
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bioRxiv - Physiology 2019Quote: ... the culture sample was diluted with the DNA/RNA protection reagent of the Monarch Total RNA Miniprep Kit (New England Biolabs, Ipswich, MA, USA), followed mechanical lysis with ZR BashingBead™ Lysis Tube (0.5mm ...
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bioRxiv - Physiology 2019Quote: ... The synthesis of cDNA for reverse transcription was carried out with a Protoscript II first strand cDNA synthesis kit (New England Biolabs, Ipswich, MA, USA) using 120 ng total RNA and 60 μM random hexamers ...
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bioRxiv - Physiology 2020Quote: ... Sequencing library was prepared using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (New England Biolabs Inc., Cat # E7770S) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... sgRNAs were synthesised directly from gBlock® (IDT) templates containing the T7 promoter using the HiScribe™ T7 high yield RNA synthesis kit (New England BioLabs®) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Digoxigenin (DIG)-labelled anti-sense and sense RNA probes corresponding to GPA2 and GPB5 subunits were synthesized using the HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, Whitby, ON, Canada). Fluorescence in situ hybridization (FISH ...
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bioRxiv - Genomics 2019Quote: ... Library preparation for sequencing was performed using a NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (E7645, New England Biolabs NEB). Samples were sequenced paired-end ...
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bioRxiv - Synthetic Biology 2019Quote: ... Cell pellets were suspended in 800 µL of DNA/RNA protecting buffer from the Monarch Total RNA MiniPrep kit (New England Biolabs, Ipswich, MA, USA), transferred into the ZR S6012-50 Bashing beads lysis tubes (Zymo Research ...
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bioRxiv - Synthetic Biology 2019Quote: ... 80 ng of RNA of each sample was converted into cDNA using the LunaScript RT SuperMix kit (New England Biolabs, Ipswich, MA, USA). qPCR experiments were performed with the Luna Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Plant Biology 2020Quote: ... and cDNA libraries were prepared using NEBNext® Ultra™ RNA Library Prep Kits for Illumina® (New England BioLabs, Ipswich, MA, USA) according to the manufacturers’ protocols ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA-seq library prep was done with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs cat. no. E7420) per the manufacturer’s standard protocol.
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bioRxiv - Evolutionary Biology 2020Quote: ... dual-indexed Illumina genomic libraries were prepared for each DNA sample using an insert size of ~350 bp and the Ultra II Library Prep Kit (New England BioLabs, Ipswich, MA, USA), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: The RNA-Seq libraries were prepared using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs, Ipswick MA), following manufacture’s recommendation ...
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bioRxiv - Molecular Biology 2020Quote: ... The amplicons were gel purified and 100 ng of the mutated linearized plasmids were re-ligated by using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB, Ipswich, MA, USA), following the manufacturers’ protocol ...
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bioRxiv - Zoology 2020Quote: ... of the reaction was then checked for successful amplification on a 1% agarose with the 1 kb DNA Ladder from New England Biolabs and afterwards the remaining PCR product was purified using the Monarch® PCR & DNA Cleanup Kit (New England Biolabs; Ipswich, MA). Purified products for S ...
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bioRxiv - Zoology 2021Quote: ... a total of nine RNA libraries were prepared with 3 μg RNA using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... of which 5 μl was processed with the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (New England Biolabs) with previously published modifications to the manufacturers protocol 37 ...
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bioRxiv - Microbiology 2021Quote: ... DNA libraries were prepared in a paired-end layout with NEBNext® Ultra™ II FS DNA Library Prep Kit (New England BioLabs, UK) and sequenced under a 150-bp read length using Illumina HiSeq 4000 systems and HiSeq SBS Kit v4 reagents (Illumina ...
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bioRxiv - Pathology 2021Quote: RT-qPCR was also performed on RNA extracted from the 4 dpi lung samples with NEB Luna Universal One-Step RT-qPCR Kit with SYBR-Green (NEB, Ipswich, MA, USA) to quantify the cytokines IL-6 and IFN-β ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Genomic libraries were prepared using the NEBNext Ultra DNA Library Prep Kit with NEBNext Multiplex Oligos for Illumina (New England Biolabs, Ipswich, MA, USA) to enable multiplexed sequencing ...
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bioRxiv - Genomics 2021Quote: ... Fragmented gDNA was ligated to NEBNext Adaptor for Illumina using NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs, cat. No. E7645). We performed bisulfite conversion twice with ligated libraries (QIAGEN ...
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bioRxiv - Genetics 2020Quote: ... Sheared genome DNA was used to build the library using NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (E7370/7335, NEB) and Illumina sequenced by Ribobio in Wuhan ...
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bioRxiv - Genetics 2020Quote: ... 17-25 nt small RNA fractions were isolated by denaturating PAGE and subjected to standard small RNA library preparation using the NEB Next small RNA sequencing Kit (NEB, Frankfurt a.M., Germany). The procedure includes 3′-OH and 5′-monophosphate specific ligation steps and we tried to lower 3′-2′-O-me biases by 18 hours 3′-ligation at 16°C ...
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bioRxiv - Cell Biology 2021Quote: ... two-step RT-qPCR was performed using LunaScript® RT SuperMix Kit (E3010L) for cDNA synthesis and Luna® Universal qPCR Master Mix (NEB #M3003) for RT-qPCR ...
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bioRxiv - Genomics 2021Quote: ... the isolated RNA was used for cDNA library construction using the NEBNext Ultra RNA Library Preparation Kit for Illumina (New England Biolabs, Ipswich, MA, USA), with a fragment length of approximately 150 bp ...
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bioRxiv - Biochemistry 2021Quote: ... and one plasmid was randomly selected from those plasmids and used for expression with a PURE system (PURExpress In Vitro Protein Synthesis Kit, New England BioLabs, Ipswich, MA, USA).
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Cryo-EM reveals disrupted human p97 allosteric activation by disease mutations and inhibitor bindingbioRxiv - Biochemistry 2021Quote: ... c.1774G>A (D592N) (see Table S1 for full list of primer sequences) using the Q5® Site-directed mutagenesis kit (New England Biolabs, MA, USA). Each mutant sequence was verified for insertion of the correct mutation using sanger sequencing.
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bioRxiv - Microbiology 2021Quote: ... One microgram of RNA from each pool was used for library preparation with the NEBNext® UltraTMRNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were prepared by the Cornell Transcriptional Regulation and Expression (TREx) Facility using the NEBNext Ultra II Directional RNA kit (New England Biolabs, E7760, Ipswich, MA). Sequencing was performed at the Biotechnology Research Center at Cornell University on the NextSeq500 (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were prepared by the Cornell Transcriptional Regulation and Expression (TREx) Facility using the NEBNext Ultra II Directional RNA kit (New England Biolabs, E7760, Ipswich, MA). Sequencing was performed at the Biotechnology Research Center at Cornell University on the NextSeq500 (Illumina ...
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bioRxiv - Biochemistry 2021Quote: ... The amplified DNA fragment was cloned into BamHI and SalI digested pGST parallel1 vector (Sheffield et al.,1999) with an NEBuilder HiFi DNA Assembly kit (NEB, Ipswich, MA, USA).
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bioRxiv - Developmental Biology 2021Quote: Total RNA was extracted from four caudal fins per experimental point using a Monarch Total RNA miniprep kit (#T2010S; New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... samples were diluted in 10 μl of RNase-free water and 5 μl of the sample were used for RNA library construction using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... Ribosomal RNA was firstly removed and a directional sequencing library was constructed using NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... All libraries were prepared using NEBNext Poly(A) mRNA magnetic isolation modules and Ultra II Directional Library Prep kits (New England BioLabs, Inc, MA, USA) according to standard protocol ...
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bioRxiv - Genomics 2021Quote: RNA-seq libraries were constructed at the ICBR Gene Expression & Genotyping Core Lab using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Samples were prepped for high-throughput sequencing using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... cDNA strand synthesis and indexing were carried out using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs) according to the supplier’s manual ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 S-FL K-to-R and SARS-CoV-2 S-Truncated K-to-R) were generated using the Q5® site-directed mutagenesis kit (New England BioLabs Inc., #E0552S) or the QuickChangeSite-Directed Mutagenesis kit (Stratagene ...
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bioRxiv - Molecular Biology 2021Quote: ... The digested products were separated by 0.8% agarose gel electrophoresis and purified using the Monarch® DNA Gel Extraction Kit (New England Biolabs, Ipswich, MA, USA). The purified products were ligated using T4 DNA ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were prepared from ∼500ng of input or IP DNA using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB) according to kit instructions ...
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bioRxiv - Immunology 2021Quote: ... DNA oligomers with Golden Gate overhangs were annealed and subsequently cloned into the non-digested target plasmid using the NEB® Golden Gate Assembly Kit (BsmBI-v2, New England Biolabs cat E1602L). sgRNAs have been cloned into pXPR_502 (addgene 96923 ...