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Citations for New England Biolabs :
8801 - 8850 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Purified DNA was used to generate libraries using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs cat# 7370) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were prepared using NEBNext Ultra II Directional RNA library preparation kit for Illumina (New England Biolabs GmbH, Frankfurt am Main, Germany). Libraries were sequenced to the depth of at least 60 million reads ...
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bioRxiv - Cancer Biology 2023Quote: ... 800 ng of RIN ≥7 of total RNA was used for library construction using a NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... DNA was sheared to fragments of ∼300 bp and libraries were created using the NEBNext Ultra DNA Library Prep Kit (New England Biolabs, Ipswich, MA). An Illumina NextSeq 500 sequencer (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... SCAR-seq libraries were prepared following the previous published protocol (11) without any modification except using NEBNext® Ultra™ II DNA library prep kit (NEB) for the end repair ...
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bioRxiv - Biochemistry 2023Quote: ... respective FoxP3 CDS with overhangs of pcDNA vector was synthesized by IDTDNA and then assembled with NEBuilder® HiFi DNA Assembly Cloning Kit (NEB, #5520G). FoxP3 paralogs (FoxP1 ...
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bioRxiv - Plant Biology 2023Quote: ... from Col-0 cDNA and prepared for in vitro transcription with the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA). After purification with Monarch RNA Cleanup Kit (New England Biolabs ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesized from 1 μg total RNA using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, catalog #E6560S) with oligo-dT priming according to the manufacturer protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-Seq libraries are prepared by Integragen SA (Evry, France) with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina according to supplier recommendations (NEB). The capture is then performed on cDNA libraries with the Twist Human Core Exome +Custom IntegraGen Enrichment System according to supplier recommendations (Twist Bioscience) ...
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bioRxiv - Cell Biology 2023Quote: ... The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA). Briefly ...
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bioRxiv - Plant Biology 2024Quote: ... 36 sequencing libraries were obtained using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (New England Biolabs, MA, USA), followed by PE 150 sequencing on the Illumina Novaseq 6000 ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were prepared using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs Japan, Tokyo, Japan) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... in vitro translation of the short-model mRNA was performed using a PURExpress® In VitroProtein Synthesis Kit (New England Biolabs, US). The reaction mixture (volume ...
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bioRxiv - Microbiology 2023Quote: RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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Pre-implantation genome-wide methylation enables environmental adaptation in a social meso-carnivorebioRxiv - Genomics 2024Quote: Genomic DNA was extracted and purified from 200 μL of whole blood using Monarch Genomic DNA Purification Kits (New England Biolabs, United Kingdom). DNA quantity and quality were assessed using NanoDrop 2000 (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Indexed cDNA libraries were obtained using the NEBNext Ultra II directional RNA Library Prep Kit for Illumina® E7760L (NEW ENGLAND BioLabs Inc) following manufacturer recommendations ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we extracted high molecular weight DNA from brain and liver tissue with the NEB Monarch HMW DNA Extraction Kit for Tissue (Catalog #T3060S, NEB, Ipswich, Massachusetts). The purified DNA was size selected for >40kb fragments with the PacBio SRE XL buffer (Catalog #102-208-400 ...
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bioRxiv - Immunology 2024Quote: ... and the cDNA was generated using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (New England Biolabs, #E7770S). Next ...
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bioRxiv - Genetics 2024Quote: RNA sequencing was performed upon libraries constructed using the NEB stranded mRNA-seq library preparation kit (New England Biolabs, Ipswich, MA, USA). RNA libraries were sequenced as 150bp pair-end fragments using Illumina Novaseq system (Illumina ...
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bioRxiv - Genetics 2024Quote: ... sgRNA complementary to the central part of the hACE2 (Table 2) was synthesized with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, USA). Library was loaded onto the FLO-MIN106 flow cell and sequenced with GridION (Oxford Nanopore ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR reactions were run on a 1% agarose gel and positive bands were excised and purified using Monarch Gel extraction clean kit (NEB, Cat. #T1020L). A-tailing of the fragments was carried out by incubating the DNA with MyTaq polymerase (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... An RNA-seq library was prepared using an NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA) after ribosomal RNA (rRNA ...
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bioRxiv - Cell Biology 2024Quote: ... The cDNA library was constructed with mean inserts of 200bp with non-stranded library preparation using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, cat#E7530L). Sequencing was performed by a paired-end 125 cycles rapid run on the Illumina HiSeq2500 ...
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bioRxiv - Genomics 2024Quote: ... The purified Hi-C DNA was sheared by covaries E220 evolution and libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB, no. E7645), and the average size of the library was detected by bioanalyzer ...
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bioRxiv - Genomics 2024Quote: Genomic DNA was used in library preparations utilizing the NEB Next® Ultra DNA Library Prep Kit for Illumina® (NEB, USA) following the recommended protocols ...
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bioRxiv - Microbiology 2024Quote: ... Paired-end sequence libraries were prepared using the NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (New England BioLabs Inc. ...
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bioRxiv - Immunology 2024Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and the sequencing library was generated using the NEBNext® Ultra™II Directional RNA Library Prep Kit (New England Biolabs, E7760). RNA-seq was performed on NovaSeq 6000 (Illumina ...
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bioRxiv - Cell Biology 2024Quote: Site directed mutagenesis was performed using the Q5® Site-Directed Mutagenesis Kit according to the manufacturer’s instruction (New England BioLabs Inc. (NEB), Ipswich ...
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bioRxiv - Cell Biology 2024Quote: RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Immunology 2024Quote: ... The T7 sgRNA PCR product (500 ng) was used as a template for in vitro transcription with the T7 High Yield RNA synthesis kit (NEB, cat # E2040S). The reaction was incubated at 37°C for 4 hours ...
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bioRxiv - Immunology 2024Quote: ... Illumina compatible cDNA libraries generated for polyA selected mRNA using NEB next ultra RNA library preparation kit Cat#E7530L (New England Biolabs Inc, MA). The cDNA libraries were purified using AmpureXP beads ...
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bioRxiv - Neuroscience 2024Quote: ... and 50 ng of total RNA was used for RT-qPCR using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, cat# E3005L) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... and the linear backbone vector (5098 base pairs in size) was gel extracted using a gel extraction kit (NEB, Cat. No. T1020S). The second product from the digestion (2878 base pairs ...
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bioRxiv - Bioengineering 2024Quote: The two fragments from pCpf1b-sp2 plasmid and the gRNA cassette obtained above was assembled by using Golden Gate Assembly (Golden Gate Assembly Kit, New England BioLabs, and E1601S). The cycling conditions were as follows ...
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bioRxiv - Microbiology 2024Quote: ... input and immunoprecipitated DNA were prepared into multiplexed libraries using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA). For the RNA-seq library ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Fully homogenized tissue was then used for DNA purification using the “Protocol for Extraction and Purification of Genomic DNA from Tissues” protocol for extraction and purification of genomic DNA from tissues in the Monarch® Genomic DNA Purification Kit (New England Biolabs T3010). DNA extract quality was assessed for quality (260:230 nm and 260:280 nm wavelengths ...
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bioRxiv - Genomics 2024Quote: Each cDNA library was built from 300 ng total RNA with ERCC spike-ins (Thermo cat. #4456740) using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB cat. #E7760), specifically the protocol for use with NEBNext Poly(A ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 µg total RNA was used as input to NEBNext Poly(A) mRNA Magnetic Isolation Module for NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Cat #E7775), following the manufacturer’s protocol and using 7 PCR cycles for library amplification ...
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bioRxiv - Neuroscience 2024Quote: ... Isolated RNA from brain regions and purified microglia was converted to cDNA and prepared for Real Time quantitative PCR (RT-qPCR) reactions using the LunaScript® RT SuperMix Kit (New England Biolabs E3010) and Luna® Universal qPCR Master Mix (New England Biolabs M3003) ...
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bioRxiv - Molecular Biology 2024Quote: ... a two-step process was used in which cDNA was first synthesized with oligo dT priming using the ProtoScript II First-Strand cDNA Synthesis kit (New England BioLabs, Ipswich, MA). To denature the template RNA ...
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bioRxiv - Biochemistry 2024Quote: ... The concentration of adapter-labelled DNA fragments in each sample was measured via qPCR using NEBNext® Library Quant Kit for Illumina® (NEB). The samples were then pooled together to an equimolar concentration and checked on a Tapestation (Agilent).
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bioRxiv - Plant Biology 2024Quote: ... cDNA libraries were constructed using a NEBNext Ultra II RNA Library Prep Kit with Sample Purification Beads (New England Biolabs, Tokyo, Japan), a NEBNext Poly(A ...
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bioRxiv - Biochemistry 2024Quote: ... DNA digestion was performed with DNase I and RNA was reverse-transcribed into cDNA using LunaScriptTM RT SuperMix kit (New England Biolabs, Cat#E3010L). qPCR was performed using the StepOnePlusTM Real-Time PCR System with SYBR green as the detection method ...
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bioRxiv - Bioengineering 2024Quote: ... Reactions were incubated at 16°C for 16 hours and purified with Monarch® RNA Cleanup Kits (New England Biolabs, Cat# T2030) according to manufacturer instructions ...
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bioRxiv - Genomics 2024Quote: ... with library preparation conducted using the NEBNext® Ultra™ IIDNA Library Prep Kit (from New England BioLabs Inc., Cat No. E7645). Ethical approval was obtained from the medical research ethics committees of the Ministry of Health ...
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bioRxiv - Evolutionary Biology 2024Quote: The RNA prepared as described above was sent to Macrogen Japan for library preparation and cDNA sequencing as follows: ribosomal RNA (rRNA) was removed from each sample using the NEBNext Bacteria rRNA Depletion Kit (New England BioLabs, Ipswich, MA), and library preparation was performed using the TruSeq Stranded Total RNA Library Preparation Kit (Illumina) ...
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bioRxiv - Cancer Biology 2024Quote: ... The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... using the NEBNext® Ultra TM II DNA Library Prep Kit (NEB E7645S/L, New England BioLabs ® Inc., Ipswich, MA, USA) as described previously (6 ...