Labshake search
Citations for New England Biolabs :
8351 - 8400 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2023Quote: ... samples were split into 5 μg aliquots for sequencing library preparation using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, E7645L) and NEBNext Multiplex Oligos (New England Biolabs ...
-
bioRxiv - Genomics 2023Quote: ... The linear backbone vector (2,183 base pairs in size) was gel extracted using a gel extraction kit (NEB, Cat. No. T1020S) and assembled with the pegRNA oligos (listed above ...
-
bioRxiv - Genomics 2023Quote: ... In vitro transcription of pegRNAs and gRNAs was performed using the HiScribe SP6 RNA Synthesis Kit (New England Biolabs, no. E2070S), purified using the Monarch RNA Cleanup Kit (New England Biolabs ...
-
bioRxiv - Cell Biology 2023Quote: ... The digested fragments were gel-purified and then cleaned up using a GenepHlow™ Gel/PCR Kit before ligation into the appropriate vector using T4 ligase (New England Biolabs). The ligated plasmids were transformed into DH5α ...
-
bioRxiv - Cell Biology 2023Quote: ... G3BP1-GFP (V11A, F112A, F33A, F33W, F15A, F15W, F124A, F124W) were created by the Q5 Site-Directed Mutagenesis kit (NEB; E0554S).
-
bioRxiv - Developmental Biology 2023Quote: ... Libraries for RNA-Seq were prepared using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, E7760) and sequenced on the NextSeq500 (Illumina ...
-
bioRxiv - Bioengineering 2023Quote: ... RNA-seq libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, Cat. No. / ID: E7770) following the manufacturer’s protocols ...
-
bioRxiv - Molecular Biology 2023Quote: ... full length CDS of TaSnRK1α gene was amplified using the wheat cDNA and cloned into the pCAMBIA1302 vector under the constitutive promoter 35S using the Gibson assembly cloning kit (Gibson Assembly® Master Mix, NEB) with the help of restriction enzyme NcoI ...
-
bioRxiv - Neuroscience 2023Quote: ... RNA-Seq was performed using libraries prepared with NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced on the Illumina NextSeq 500 platform to obtain 36bp paired-end reads ...
-
bioRxiv - Neuroscience 2023Quote: ... ChIP-Seq was performed using libraries prepared with the NEBNext Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs) as per the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2023Quote: ... we introduced a silent mutation into the PAM motif of the sgRNA located within the 3’ homology arm in the donor plasmid by using Q5 Site-Directed Mutagenesis kit (NEB, E0054). The donor plasmid was confirmed with DNA sequencing ...
-
bioRxiv - Microbiology 2023Quote: ... Corresponding site-directed mutagenesis was conducted on the intermediate TOPO constructs with specific mutation primers by using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs). The mutated E2- or E1-containing fragments were digested from the TOPO constructs through intrinsic viral restriction sites and inserted back into CHIKV through T4 ligation.
-
bioRxiv - Molecular Biology 2023Quote: ... from 1 µg of total RNA and library preparation was conducted with the NEBNext® Ultra RNA Library Prep Kit for Illumina (NEB) with Dual Index Primers (NEBNext® Multiplex Oligos for Illumina ...
-
bioRxiv - Neuroscience 2023Quote: ... RNA libraries were prepared using NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England Biolabs) and sequenced using the Illumina NOVAseqSP sequencer.
-
bioRxiv - Microbiology 2023Quote: ... Parasites were pelleted by centrifugation at 13,000 xg for five minutes and the parasite DNA was isolated using the Monarch® Genomic DNA Purification Kit (New England Biolabs).
-
bioRxiv - Microbiology 2023Quote: RNA was used as template to detect and quantify viral genomes by duplex reverse transcriptase (RT) quantitative polymerase chain reaction (RT-qPCR) using a Luna Universal Probe one-step RT-qPCR kit (New England Biolabs, E3006E). SARS-CoV-2-specific RNAs were detected by targeting the ORF1ab gene using the following set of primers and probes ...
-
bioRxiv - Microbiology 2023Quote: ... the strand-specific cDNA libraries were constructed with a NEB Next Ultra II Directional RNA library preparation kit for Illumina and the NEB Next Multiplex Oligos for Illumina (96) (New England BioLabs, Germany). To assess quality and size of the libraries ...
-
bioRxiv - Physiology 2023Quote: ... which were then used to replace the corresponding regions in the wildtype plasmids with NEBuilder® HiFi DNA Assembly Cloning Kit (New England BioLabs). All plasmids were fully sequenced ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cDNAs were subcloned downstream of a tetracycline response element (TRE) using the Gibson Assembly Cloning kit (New England Biolabs, # E5510S). Mutations were introduced by in vitro mutagenesis using Q5 Site-directed Mutagenesis Kit (New England Biolabs ...
-
bioRxiv - Plant Biology 2023Quote: ... RNA extraction was done with TriZol from the 12 fractions collected and then samples were cleaned up using the Monarch® RNA Cleanup Kit (50 μg) from New England Biolabs (NEB). Optical densities (OD260 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... we constructed the genetic sequence of the major shell protein of 50nmGVs using Q5 DNA polymerase and Gibson assembly kits (New England Biolabs Inc.)65 ...
-
bioRxiv - Plant Biology 2023Quote: Constructs of MBP-DspE and MBP-DspEΔβ-barrel were made by the infusion cloning methods using NEBuilder HiFi® DNA assembly kit (New England Biolabs) with the following primer sets:
-
bioRxiv - Microbiology 2023Quote: ... For in vitro transcription plasmids were first linearised by restriction digest and purified using Monarch PCR and DNA Clean-up Kit (NEB, USA). 3’UTRs were in vitro transcribed from 500ng of plasmid DNA using MEGAscript T7 Transcription Kit (Invitrogen ...
-
bioRxiv - Plant Biology 2023Quote: ... The purified total RNA was used to synthesize cDNA using LunaScript® RT SuperMix Kit (New England Biolabs, Catalog number E3010). The synthesized cDNA was analyzed using qPCR using Luna® Universal qPCR Master Mix (NEB ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... or NEBNext Ultra FS DNA library kits (paired-end 150bp reads, average insert size 155 ± 105 bp, New England Biolabs, MA)) following the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... the BRE-Citrine was replaced with CAGA12-mCherry using the NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs, E5520S). For ease of readability these reporters are referred to as BRE-YFP and SBE-RFP in the manuscript.
-
bioRxiv - Developmental Biology 2023Quote: ... A short version of enhD1 luciferase vector was mutated with use of a gBlock (IDT) and NEBuilder HiFi DNA Assembly kit (NEB, E2621). Luciferase reporter vectors were co-transfected with pNL1.1.PGK[Nluc/PGK] (N1441 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Sequencing libraries were generated with a NEBNext Ultra Directional RNA Library Prep kit according to the supplier’s directions (New England Biolabs, Beverly, MA). Sequencing was performed as previously described on a NovaSeq 600 instrument (Illumina ...
-
bioRxiv - Genetics 2023Quote: ... strand-specific mRNA libraries were generated using the NEBNext Ultra II Directional RNA library prep Kit for Illumina (New England BioLabs #E7760), and mRNA was isolated using Poly(A ...
-
bioRxiv - Genetics 2023Quote: These three parts were assembled together with the pJmp3 backbone using the NEBuilder HiFi DNA Assembly cloning kit (NEB cat # E5520S) and transformed to DH5α competent cells ...
-
bioRxiv - Microbiology 2023Quote: All parasite materials were centrifuged at 1,000 g for 2 min and total genomic DNA was isolated from the resulting pellets using the Monarch Genomic DNA Purification kit (New England Biolabs, Australia). For the ovine ...
-
bioRxiv - Genetics 2023Quote: ... 100ng of each mRNA sample was used to prepare libraries with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB E7760S) according to the manual ...
-
bioRxiv - Genomics 2023Quote: ... with the exception that elution of the Tn5 treated DNA was performed using the Monarch PCR and DNA clean-up kit (NEB T1030S) following a 5:1 ratio of Binding Buffer:Sample ...
-
bioRxiv - Genomics 2023Quote: ... the samples were incubated with 20-fold diluted Quick Ligase in 1× Quick Ligase Reaction Buffer from Quick Ligation Kit (NEB M2200) supplemented with additional 1 mM ATP (NEB P0756 ...
-
bioRxiv - Microbiology 2023Quote: ... Genomic DNA was sonicated using Qsonica sonicator (Q8000R) and the sonicated DNA was used to prepare libraries using the NEBNext UltraII DNA library prep kit (NEB #E7645L). Paired end sequencing of the libraries was performed using Illumina NextSeq 550 platform and at least 3 million paired end reads were obtained for each sample ...
-
bioRxiv - Molecular Biology 2023Quote: ... truncations were then constructed from this plasmid by site-directed deletion of the appropriate regions of the SFPQ ORF using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs). The mammalian expression construct encoding GFP-tagged full-length SFPQ was described previously (Huang et al ...
-
bioRxiv - Cancer Biology 2023Quote: ... The U6 promoter was deleted and replaced with an XbaI site using NEB Q5 site directed mutagenesis kit (NEB Cat# E0554S) with primers forward ...
-
bioRxiv - Genetics 2023Quote: ... and inserted to AatII site on pHACK-GAL4>nlsLexA::GADfl (v1) using the HiFi DNA Assembly Cloning Kit (New England BioLabs, E5520S). The resulting construct carrying both 3xP3-RFP and yellow transgene markers ...
-
bioRxiv - Microbiology 2023Quote: ... AOB and AOA amoA genes were created using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, MA) according to the manufacturer’s guideline ...
-
bioRxiv - Plant Biology 2023Quote: ... The DNA fragment was introduced into pGreen0179_YFP-(AauI)::35St with EXP7a promoter by using a NEBuilder HiFi DNA Assembly kit (NEB, MA, USA), and the resulting construct was transformed into Arabidopsis Col-0 by floral dip (Clough and Bent ...
-
bioRxiv - Genomics 2023Quote: ... Sequencing libraries for genome wide DNA methylation profiles were generated from 200 ng of genomic DNA using NEBNext® Enzymatic Methyl-seq Kit (New England Biolabs). These libraries were sequenced on an Illumina NextSeq 2000 to generate 100 bp paired end reads.
-
bioRxiv - Microbiology 2023Quote: All clonings were carried on in a Gibson assembly-like reaction with the NEBuilder® HiFi DNA Assembly kit (NEB, E2621) and into plasmids derived from pMM704 or pMM731 (17) ...
-
bioRxiv - Bioengineering 2023Quote: mRNA was synthesized through in vitro transcription using the HiScribe T7 mRNA Kit with CleanCap Reagent AG (NEB, China, Cat: E2080S). The templates for in vitro transcription were linearized plasmids ...
-
bioRxiv - Evolutionary Biology 2023Quote: RNA libraries from at least three replicate individuals per sex for each lineage (Table S1) were prepared from gametes using NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs). Mature gametophytes release gametes just after the medium renewal ...
-
bioRxiv - Developmental Biology 2024Quote: ... Purified RNAs were used for Illumina RNA-seq library preparation with NEBNext Ultra II Directional RNA Library Prep Kit (NEB; E7765), and a minimum of 20 million raw reads were obtained ...
-
bioRxiv - Developmental Biology 2024Quote: ... RNA was quantified by Nanodrop and 1 µg of RNA per sample was treated for ribosomal RNA depletion using the NEBNext rRNA Depletion Kit (NEB, E6350) and then immediately followed by Library prep using NEBNext Ultra II kit (NEB ...
-
bioRxiv - Neuroscience 2022Quote: ... and used to generate point mutations in the GlyT2 cDNA/pOTV construct utilising the Q5 site-directed mutagenesis kit (New England Biolabs (Genesearch), Arundel ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Purified DNA was eluted in 30 μl of Ultrapure water and 10ng was inputted into the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S) using the following PCR programme ...
-
bioRxiv - Molecular Biology 2022Quote: ... and 500 ng of the gDNA was subjected to DNA library preparation using the NEBNext Ultra II FS DNA Library Prep kit for Illumina (New England Biolabs, E7805S) following manufacturer’s instructions (protocol for inputs >100 ng) ...
-
bioRxiv - Neuroscience 2022Quote: ... Deletion mutant EGFP-Inka2ΔiBox and EGFP-Inka2ΔCC were constructed from pEGFP-Inka2 using Q5 Site-Directed Mutagenesis Kit (New England BioLabs, Ipswich, MA, USA). Expression plasmids for Pak1 (pCMV6M-Pak1 ...