Labshake search
Citations for New England Biolabs :
8201 - 8250 of 9501 citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... NEBNext Low Input RNA libraries were prepared manually following the manufacturer’s protocol (NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® Instruction Manual Version 5.0_5/20, NEB #E6420L). Samples were normalized to 1ng total RNA material per library in 8μl of nuclease-free water ...
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bioRxiv - Developmental Biology 2023Quote: ... building parallel total-RNA and poly(A)+ Illumina strand-specific libraries using the Ultra II RNA-seq library kit (NEB #E7765) (see below) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Linear plasmid fragments were generated by PCR and purified by Monarch® PCR and DNA cleanup kit (New England BioLabs®). Ligations were performed with In-Fusion® enzyme at 50°C for 15 minutes (min ...
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bioRxiv - Synthetic Biology 2023Quote: The cell-free protein synthesis (CFPS) reactions were carried out using the PURExpress In Vitro Protein Synthesis Kit (New England Biolabs Inc) in 384-well plates (Thermofisher NUNC ...
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bioRxiv - Systems Biology 2022Quote: ... A total of 500ng of purified RNA was used as input for the NEBNext Ultra II RNA Library Prep Kit (NEB #E7770S), which first involved enrichment of polyadenylated mRNA using the NEBNext Poly(A ...
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bioRxiv - Biochemistry 2022Quote: ... were generated from pGB1-MITF81-204 and pGAL4-MITF1-204 using the Q5® QuickChange site-directed mutagenesis kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... 8 µl PCR amplified product was used for the in vitro transcription using the NEB HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, E2040S), incubating at 37 °C for 18 hours in the thermocycler ...
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bioRxiv - Neuroscience 2023Quote: ... NEBNext Ultra RNA Library Prep Kit was then used to prepare sequencing libraries according to manufacturer’s instructions (NEB, Ipswich, MA, USA). Oligo(dT ...
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bioRxiv - Immunology 2023Quote: ... Stranded RNA sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, cat# E7760L). Purified libraries were qualified on an Agilent Technologies 4150 TapeStation using a D1000 ScreenTape assay (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... Paired-end Illumina sequencing libraries were prepared from CUT&RUN enriched DNA using the NEBNext Ultra™ II DNA Library Prep Kit (New England Biolabs) and sequenced on a NextSeq 500 (Illumina) ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were harvested by phenol-chloroform extraction and processed according to the NEB Ultra II Directional RNA Sample Prep Kit (New England Biolabs, #E7760S).
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (New England Biolabs, MA, USA). Illumina MiSeq sequencing was carried out on the IlluminaHiseq2500 platform (Illumina Inc. ...
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bioRxiv - Microbiology 2023Quote: ... RNA was diluted 1:50 for RT-qPCR which was conducted using the Luna Universal One-Step RT-qPCR kit (New England Biolabs E3005E) on a C1000 touch thermal Cycler / CFX384 Real-Time System (Biorad) ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was purified from 100μL of CSF samples using the Monarch® Genomic DNA Purification Kit (New England Biolabs, Australia) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Substrates were transcribed in vitro from purified PCR products using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... strand-specific mRNA libraries were generated using the NEBNext Ultra II Directional RNA library prep Kit for Illumina (New England BioLabs #E7760), and mRNA was isolated using Poly(A ...
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bioRxiv - Genetics 2023Quote: These three parts were assembled together with the pJmp3 backbone using the NEBuilder HiFi DNA Assembly cloning kit (NEB cat # E5520S) and transformed to DH5α competent cells ...
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bioRxiv - Microbiology 2023Quote: All parasite materials were centrifuged at 1,000 g for 2 min and total genomic DNA was isolated from the resulting pellets using the Monarch Genomic DNA Purification kit (New England Biolabs, Australia). For the ovine ...
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bioRxiv - Genetics 2023Quote: ... 100ng of each mRNA sample was used to prepare libraries with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB E7760S) according to the manual ...
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bioRxiv - Genomics 2023Quote: ... with the exception that elution of the Tn5 treated DNA was performed using the Monarch PCR and DNA clean-up kit (NEB T1030S) following a 5:1 ratio of Binding Buffer:Sample ...
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bioRxiv - Genomics 2023Quote: ... the samples were incubated with 20-fold diluted Quick Ligase in 1× Quick Ligase Reaction Buffer from Quick Ligation Kit (NEB M2200) supplemented with additional 1 mM ATP (NEB P0756 ...
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bioRxiv - Biochemistry 2023Quote: ... 0.5 μg of linearized plasmid was used as template in a 10 µL reaction using the HiScribe T7 High Yield RNA Synthesis Kit (NEB # E2040S) with an 8:1 ratio of cap analog to GTP ...
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bioRxiv - Cell Biology 2023Quote: ... the BRE-Citrine was replaced with CAGA12-mCherry using the NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs, E5520S). For ease of readability these reporters are referred to as BRE-YFP and SBE-RFP in the manuscript.
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bioRxiv - Developmental Biology 2023Quote: ... A short version of enhD1 luciferase vector was mutated with use of a gBlock (IDT) and NEBuilder HiFi DNA Assembly kit (NEB, E2621). Luciferase reporter vectors were co-transfected with pNL1.1.PGK[Nluc/PGK] (N1441 ...
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bioRxiv - Molecular Biology 2023Quote: ... 100ng of polyA selected mRNA samples were treated using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB E7760S) according to the manual ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the pML104-GAL1 vector has a guide sequence 5’- CTCTTAAATTATAGTTGGTT-3’ introduced by Q5® Site-Directed Mutagenesis Kit (New England Biolabs) (Hu et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Sequencing libraries were generated with a NEBNext Ultra Directional RNA Library Prep kit according to the supplier’s directions (New England Biolabs, Beverly, MA). Sequencing was performed as previously described on a NovaSeq 600 instrument (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... The U6 promoter was deleted and replaced with an XbaI site using NEB Q5 site directed mutagenesis kit (NEB Cat# E0554S) with primers forward ...
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bioRxiv - Genetics 2023Quote: ... and inserted to AatII site on pHACK-GAL4>nlsLexA::GADfl (v1) using the HiFi DNA Assembly Cloning Kit (New England BioLabs, E5520S). The resulting construct carrying both 3xP3-RFP and yellow transgene markers ...
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bioRxiv - Plant Biology 2023Quote: ... The DNA fragment was introduced into pGreen0179_YFP-(AauI)::35St with EXP7a promoter by using a NEBuilder HiFi DNA Assembly kit (NEB, MA, USA), and the resulting construct was transformed into Arabidopsis Col-0 by floral dip (Clough and Bent ...
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bioRxiv - Genomics 2023Quote: ... Sequencing libraries for genome wide DNA methylation profiles were generated from 200 ng of genomic DNA using NEBNext® Enzymatic Methyl-seq Kit (New England Biolabs). These libraries were sequenced on an Illumina NextSeq 2000 to generate 100 bp paired end reads.
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bioRxiv - Microbiology 2023Quote: ... AOB and AOA amoA genes were created using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, MA) according to the manufacturer’s guideline ...
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bioRxiv - Molecular Biology 2023Quote: ... truncations were then constructed from this plasmid by site-directed deletion of the appropriate regions of the SFPQ ORF using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs). The mammalian expression construct encoding GFP-tagged full-length SFPQ was described previously (Huang et al ...
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bioRxiv - Neuroscience 2023Quote: ... The gRNAs were cloned into a dual gRNA pCFD4 vector having a BbsI restriction site using Gibson Assembly Kit (New England Biolabs (UK) Ltd ...
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bioRxiv - Microbiology 2023Quote: All clonings were carried on in a Gibson assembly-like reaction with the NEBuilder® HiFi DNA Assembly kit (NEB, E2621) and into plasmids derived from pMM704 or pMM731 (17) ...
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bioRxiv - Bioengineering 2023Quote: mRNA was synthesized through in vitro transcription using the HiScribe T7 mRNA Kit with CleanCap Reagent AG (NEB, China, Cat: E2080S). The templates for in vitro transcription were linearized plasmids ...
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bioRxiv - Neuroscience 2023Quote: ... RNA-Seq was performed using libraries prepared with NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced on the Illumina NextSeq 500 platform to obtain 36bp paired-end reads ...
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bioRxiv - Neuroscience 2023Quote: ... ChIP-Seq was performed using libraries prepared with the NEBNext Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs) as per the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... we introduced a silent mutation into the PAM motif of the sgRNA located within the 3’ homology arm in the donor plasmid by using Q5 Site-Directed Mutagenesis kit (NEB, E0054). The donor plasmid was confirmed with DNA sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was purified by PolyA selection and libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit (NEB, E7770). Samples were sequenced on a NextSeq 2000 (Illumina).
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bioRxiv - Genetics 2023Quote: RNA was isolated from frozen hippocampal samples stored at -80°C using the Monarch® RNA Total Isolation kit (NEB T2010S). Hippocampus from the left hemisphere was used where possible ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNAs were subcloned downstream of a tetracycline response element (TRE) using the Gibson Assembly Cloning kit (New England Biolabs, # E5510S). Mutations were introduced by in vitro mutagenesis using Q5 Site-directed Mutagenesis Kit (New England Biolabs ...
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bioRxiv - Plant Biology 2023Quote: ... The purified total RNA was used to synthesize cDNA using LunaScript® RT SuperMix Kit (New England Biolabs, Catalog number E3010). The synthesized cDNA was analyzed using qPCR using Luna® Universal qPCR Master Mix (NEB ...
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bioRxiv - Plant Biology 2023Quote: Constructs of MBP-DspE and MBP-DspEΔβ-barrel were made by the infusion cloning methods using NEBuilder HiFi® DNA assembly kit (New England Biolabs) with the following primer sets:
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bioRxiv - Plant Biology 2023Quote: ... RNA extraction was done with TriZol from the 12 fractions collected and then samples were cleaned up using the Monarch® RNA Cleanup Kit (50 μg) from New England Biolabs (NEB). Optical densities (OD260 ...
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bioRxiv - Synthetic Biology 2023Quote: ... we constructed the genetic sequence of the major shell protein of 50nmGVs using Q5 DNA polymerase and Gibson assembly kits (New England Biolabs Inc.)65 ...
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bioRxiv - Microbiology 2023Quote: ... For in vitro transcription plasmids were first linearised by restriction digest and purified using Monarch PCR and DNA Clean-up Kit (NEB, USA). 3’UTRs were in vitro transcribed from 500ng of plasmid DNA using MEGAscript T7 Transcription Kit (Invitrogen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... or NEBNext Ultra FS DNA library kits (paired-end 150bp reads, average insert size 155 ± 105 bp, New England Biolabs, MA)) following the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... which were then used to replace the corresponding regions in the wildtype plasmids with NEBuilder® HiFi DNA Assembly Cloning Kit (New England BioLabs). All plasmids were fully sequenced ...
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bioRxiv - Cell Biology 2023Quote: ... The digested fragments were gel-purified and then cleaned up using a GenepHlow™ Gel/PCR Kit before ligation into the appropriate vector using T4 ligase (New England Biolabs). The ligated plasmids were transformed into DH5α ...