Labshake search
Citations for New England Biolabs :
8001 - 8050 of 9501 citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: RT-LAMP reactions were performed using either WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA) (M1800) or WarmStart® LAMP Kit (DNA & RNA) (E1700) from New England Biolabs (NEB). 40 mM guanidine hydrochloride was included in all reactions to improve LAMP reaction speed and sensitivity [10] ...
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bioRxiv - Molecular Biology 2021Quote: Site-directed mutagenesis of the AP-1 binding sites in the distal enhancer reporter plasmids were performed using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs). Primer pairs 5’-CCATAATGTGgggCTATACTAAATTTCATCTTC-3’ and 5’-CTAAATCCACTTAGAAAAAACAATC-3’ ...
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Nucleo-cytoplasmic shuttling of splicing factor SRSF1 is required for development and cilia functionbioRxiv - Molecular Biology 2020Quote: ... The guide region was then amplified by PCR and paired guide RNAs synthesised by in vitro transcription (T7 Quick High Yield RNA Synthesis kit, NEB, #E2050S). Single stranded DNA oligonucleotides (WT oligo ...
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bioRxiv - Microbiology 2021Quote: ... The ChIP libraries were prepared using NEBNext® UltraTM II DNA Library Prep Kit for Illumina (Catalog: E7645S, New England Biolabs). The samples were subjected to various enzymatic steps to repair the ends and tailing with dA-tail followed by an adapter ligation ...
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bioRxiv - Neuroscience 2020Quote: ... RNA-seq libraries were prepared from total RNA using polyA selection followed by the NEBNext Ultra II Directional RNA Library Prep Kit protocol (New England Biolabs, E7760S). Sequencing was performed on Illumina HiSeq 2500 instrument resulting in approximately 30 million of 50 bp reads per sample ...
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bioRxiv - Immunology 2021Quote: ... captured mRNA was processed for Next Generation Sequencing (NGS) using the NEB Next Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, UK). Quality was assessed on the Agilent 2100 Bioanalyser using the High Sensitivity DNA Kit (Agilent ...
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bioRxiv - Microbiology 2021Quote: ... followed by adaptor ligation and enrichment with a low-cycle according to the instructions of NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA). The purified library products were evaluated using the Agilent 2200 TapeStation and Qubit2.0 (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... according to the manufacturer’s instructions.29 RNA reverse transcription and first strand cDNA synthesis were carried out using chain-specific reverse primers30 with ProtoScript® II First Strand cDNA Synthesis Kit (NEB). The resulting cDNA was used as template for preparing the sequencing library using 5’ multiplex PCR as previously described.31 The PCR products corresponding to the library amplification were purified on BluePippin with size cutoff around 500 bp ...
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bioRxiv - Cell Biology 2021Quote: ... 100 ng of total RNA was used to generate RNA-seq library using NEBNext Ultra RNA Library prep kit (NEB, E7530L) according to the vendor’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... Resultant DNA was gel extracted then 400 ng template used for in vitro transcription with the HiScribe RNA synthesis T7 kit (NEB, E2040) for 2 hours at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... BRCT domain (aa 560-777), BUDR (R705A, D712A, Q715R) were deleted or mutated using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554S). The GFP-BARD1 constructs were shuttled into a lentiviral destination vector containing a UBC promoter and a hygromycin resistance gene using the Gateway LR Clonase II Enzyme Mix (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... The digestion product was resolved on a 0.7% agarose gel and the plasmid backbone was purified using the Monarch gel purification kit (NEB, Cat# T1020S). The ‘T2A-GFP-WPRE’ sequence was inserted into the digested backbone using the Gibson Assembly kit (SGI ...
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bioRxiv - Genetics 2019Quote: ... Poly(A)+ RNA was selected from 1 μg of total RNA using the NEBNext Ultra Kit (New England Biolabs, Ipswitch, MA). Libraries were prepared and sequenced by Genewiz (South Plainfield ...
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bioRxiv - Genomics 2021Quote: ... and the fragmented DNA was subjected to library preparation with NEBNext® Ultra II DNA Library Prep Kit for Illumina (E7645S, New England Biolabs). Sequencing was carried out on Illumina NovaSeq 6000 with 150bp paired end mode.
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bioRxiv - Genomics 2021Quote: ... We prepared a total of 30 libraries using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs Inc.). We selected an insert size of 300 bp by using a fragmentation time of 5-12 min ...
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bioRxiv - Genomics 2019Quote: To prepare the RNA sample for use in a smallRNA library preparation kit the sample was phosphorylated using 5 ul of 10X T4 PNK buffer and 1 ul of T4 PNK (NEB #M0201), 1 ul of SUPERASE-In ...
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bioRxiv - Microbiology 2020Quote: ... The sequencing libraries were created using the NEBNext® Ultra™ II DNA Library Prep Kit (New England Biolabs, MA, USA). Coverage values are reported in Table 1 ...
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bioRxiv - Immunology 2022Quote: ... The immunoprecipitated DNAs were subsequently processed to generate a DNA library using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, # E7645L) and NEBNext® Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Microbiology 2022Quote: ... night RNAseq libraries were produced according to the manufacturer’s protocol (NEBNext UltraII Directional RNA Library Prep Kit, Illumina, NEB, MA, USA) using aroung 1 000 ng of total RNA ...
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bioRxiv - Microbiology 2022Quote: ... The viral cDNAs were synthesized from extracted RNA using the Luna Script RT Super Mix Kit (New England BioLabs, Ipswich, MA), followed by DNA amplification by multiplex PCR in two separated primer pools using ARTIC-N5 primers (59 ...
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bioRxiv - Immunology 2022Quote: ... Caspase-11 catalytic (C254A) and cleavage (D285A) mutants were generated by site-directed mutagenesis (Q5 SDM Kit, New England BioLabs; #E0554S) of the wild-type parent vector according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The library for sequencing was prepared using 1 µg of RNA and the NEBNext Ultra kit (New England BioLabs, Ipswich, MA), according to the protocol for low-input samples ...
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bioRxiv - Microbiology 2022Quote: ... 1-step RT-qPCR was performed on 2μL of RNA using the Luna® Universal One-Step RT-qPCR Kit (NEB Biosciences) and primers for β-tubulin (F ...
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An apical protein, Pcr2, is required for persistent movement by the human parasite Toxoplasma gondiibioRxiv - Cell Biology 2022Quote: ... and inserted between the BglII and AflII sites of ptubA1-mEmeraldFP-TUBA1[69] using the NEBuilder HiFi Assembly kit (New England Biolabs, #E2621S).
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were made with 6 ng of CUT&RUN DNA fragments using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S). The manufacturer’s protocol was adjusted to account for shorter DNA fragments as described previously (33) ...
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bioRxiv - Molecular Biology 2022Quote: ... for each co-expressed DF-APOBEC1 protein using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760L) with NEBNext Poly(A ...
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bioRxiv - Developmental Biology 2022Quote: ... equal amount of DNA (∼2 ng) was used as an input for NEB Ultra II DNA library prep kits (NEB #E7645). Number of cycles for amplification of adapter ligated libraries were estimated by the qPCR before final amplification to avoid any bias arising due to PCR amplification and indexing (NEB #E7350) ...
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bioRxiv - Biochemistry 2022Quote: ... and for OGA we introduce a D174>A mutation with a Q5 site-directed mutagenesis kit (New England Biolabs cat # E05545). These mutations produce stable mutant proteins that abolish the catalytic activity of the enzymes (38,39) ...
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bioRxiv - Cancer Biology 2022Quote: In vitro transcription of LINC00313 or firefly luciferase (F-luc) mRNA was performed using the HiScribe™ T7 High Yield RNA Synthesis kit (New England Biolabs), according to the instructions by the manufacturer ...
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bioRxiv - Biochemistry 2022Quote: ... RNA was used for RNA-Seq library generation using the NEBNext Ultra II Directional Library Prep kit for Illumina (NEB, E7760S). Amplified cDNAs were submitted for Illumina sequencing.
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bioRxiv - Biochemistry 2022Quote: ... RNA from each sample was used for RNA-Seq library generation using the NEBNext Ultra II Directional Library Prep kit for Illumina (NEB, E7760S). Amplified cDNAs were submitted for Illumina sequencing.
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bioRxiv - Biochemistry 2022Quote: ... GT GGT ATT GCC GAC GGA G) and the combination of both were generated using the Q5® site-directed mutagenesis kit (NEB) (lower case letters highlight the amino acid change) ...
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bioRxiv - Bioengineering 2022Quote: ... constant oligonucleotide was used.(Petri et al., 2022) The products were purified using Monarch® PCR & DNA Cleanup Kit (New England Biolabs), which were then used as the template for in vitro transcription ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA synthesis was carried out with a standard oligo-dT primer protocol using the ProtoScript II First Strand cDNA Synthesis Kit (NEB E6560). RNA concentrations were normalized between samples prior to reverse transcription ...
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bioRxiv - Developmental Biology 2022Quote: ... Adaptor-ligated DNA fragments of proper size were enriched with PCR reaction using Fusion High-Fidelity PCR Master Mix kit (NEB, M0531S) and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1 ...
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bioRxiv - Developmental Biology 2022Quote: ... Briefly 500pg of ribozero treated RNA was fragmented and used to produce cDNA libraries using the NEBnext Ultra RNA library prep kit (NEB, E7530S) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Library quality was assessed using an Agilent BioAnalyzer 2100 and libraries were quantified with the Library Quant Kit for Illumina (NEB #E7630L). Libraries were then diluted to 1.8pM and sequenced on the NextSeq500 platform using 75-base-pair (bp ...
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bioRxiv - Cell Biology 2022Quote: ... Sequencing libraries were prepared using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs #E6420) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... An IFT144 construct lacking the N-terminal β-propeller domain (residues 2–349 inclusive; IFT144ΔNFLAG) was made using the Q5® Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Cancer Biology 2022Quote: ... and library preparation was done using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA).56 Paired-end 150 bp read sequencing was performed ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were designed in SnapGene and mutants were generated via Gibson assembly (Gibson Assembly® Cloning Kit from New England Biolabs)57,58 or via QuickChange (Q5® Site-Directed Mutagenesis Kit from New England Biolabs).59 Recombinant expression of motors in E ...
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bioRxiv - Immunology 2022Quote: ... Short hairpin resistant chimeric mutants of Flag-tagged GLUT3 alleles were generated using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, E5520) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... were used for RNAseq library preparation with the NEBNext Single Cell / Low Input RNA Library Prep Kit for Illumina (NEB, E6420S), following the manufacturer’s protocol for single cells ...
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bioRxiv - Genetics 2022Quote: ... and DNA library preparation was performed using NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads Kit (NEB, #E6177). Whole-genome sequencing was performed using an Illumina NextSeq instrument ...
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bioRxiv - Evolutionary Biology 2022Quote: ... One µg of total RNA was used for the NEBNext® Ultra™ Directional RNA Library Prep Kit (New England Biolabs) without removing ribosomal RNA ...
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bioRxiv - Genetics 2022Quote: ... Libraries were produced with the Ultra II DNA Library prep kit according to manufacturer’s instructions (New England Biolabs, cat. no. E7645L). Bisulfite conversion of DNA was carried out according to manufacturer’s protocol (Zymo ...
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bioRxiv - Immunology 2022Quote: ... mRNAs encoding TCR genes were transcribed in vitro using the mMESSAGE mMACHINE® T7 Ultra Kit (Life) using the Avr□ (NEB) linearized plasmid DNA as templates ...
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bioRxiv - Developmental Biology 2022Quote: ... Indexed libraries were made using 0.5 μg RNA per sample with NEBNext Ultra™ RNA Library Prep Kit for Illumina with the Poly(A) mRNA Magnetic Isolation Module (NEB) and NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Genetics 2022Quote: ... 500 ng of purified PCR products (D2500, Gel Extraction Kit, OMEGA, USA) were denatured and reannealed in NEB buffer 2 (M0302S, NEB, USA): 95 ℃ ...
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bioRxiv - Genomics 2022Quote: ... IPs and INPUTS were then quantified and NGS libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, #E7645) following the manufacturer’s instructions ...