Labshake search
Citations for New England Biolabs :
8051 - 8100 of 9501 citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... from ONTs ligation sequencing kit (ONT, SQK-LSK109) and 10 µl of NEBNext Quick T4 DNA Ligase (New England Biolabs, E6056S). Ligation mix was incubated at RT for 30 min ...
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bioRxiv - Plant Biology 2022Quote: ... and single amplicon copies were ligated into the pMiniT 2.0 vector using the NEB PCR Cloning Kit (New England Biolabs, Ipswich, MA). Individual clones were selected and confirmed via colony PCR using Q5 High-Fidelity DNA Polymerase (New England Biolabs ...
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bioRxiv - Plant Biology 2022Quote: ... followed by treatment with DNase I.5 The samples were then purified with the Monarch® RNA Cleanup Kit (New England Biolabs).
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bioRxiv - Microbiology 2022Quote: crRNAs were synthesized for in vitro cleavage experiments using the HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs; NEB). Templates for in vitro transcription were generated by hybridizing a ssDNA oligo with the T7 promoter to another oligo with the complementary T7 promoter sequence fused to the LbCas12a repeat and spacer in annealing buffer ...
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bioRxiv - Microbiology 2022Quote: ... was used as a template to prepare 10 μl cDNA using LunaScript®RT SuperMix Kit (New England Biolabs, cat# E3010L) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Purified RNA was quantified by ultraviolet spectrophotometry and 1 μg used for reverse transcription by LunaScript® RT SuperMix Kit (New England Biolabs), as described by the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... The pre-existing gRNA was replaced a by the newly designed DNA using the Q5 Site-Directed mutagenesis kit (New England Biolabs, M0491S), respective primers are listed in Table 1 ...
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bioRxiv - Neuroscience 2022Quote: ... mRNA libraries were generated using the NEBNext Ultra™ II RNA Library Prep Kit for Illumina (New England BioLabs, Ipswitch, MA). Resulting libraries were sequenced on a NovaSeq 6000 at a depth of 30 million reads/sample ...
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bioRxiv - Neuroscience 2022Quote: ... Three to four independently generated PCR products for each OT1-4/founder were purified using the Monarch PCR & DNA Cleanup Kit (NEB Inc.) and sent for Sanger sequencing at the OHSU Vollum Sequence Core.
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bioRxiv - Genomics 2022Quote: ... Libraries were constructed from these sheared DNA samples using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB E7645S) and NEBNext Multiplex Oligos for Illumina Index Primers Sets 1 and 2 (NEB E7335S & E7500S) ...
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bioRxiv - Immunology 2022Quote: ... 200 ng of total RNA were used to generate barcoded RNA-seq libraries using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs Inc). Libraries were sequenced on a HiSeq 2500 (Illumina ...
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bioRxiv - Microbiology 2022Quote: Extracted DNA was used for DNA library construction using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... the CmR cassette and the downstream region were bound together by overlap PCR and purified using Monarch DNA Gel extraction kit (New England Biolabs®).
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bioRxiv - Microbiology 2022Quote: ... Purified RNAs were used for library preparation using the NEB Next Multiplex Small RNA Library Prep kit for Illumina (E7300 L) with Universal miRNA Cloning Linker from Biolabs (S1315S) as the 3’ adaptor and in-house designed indexed primers ...
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bioRxiv - Microbiology 2022Quote: RNA-free PXO99A genomic DNAs (0.2 μg) were used to construct the DNA libraries using a NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and DHX38-FKBPF36V-2xHA_P2A_BFP were synthesized with 800bp dsDNA homology arms by Genewiz and assembled using the NEBuilder HiFi DNA assembly cloning kit (NEB E5520S).
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bioRxiv - Plant Biology 2022Quote: ... The cassette was assembled to the backbone of CRISPR/Cas9 construct using Gibson Assembly® Cloning Kit (New England Biolabs Inc). The gRNAs targetingKTI1 and KTI3 were synthesized in one cassette at GenScript Biotech Corp ...
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bioRxiv - Plant Biology 2022Quote: RNA libraries were constructed using ribosomal-depleted RNAs as per the protocol of Ultra Directional RNA Library Prep Kit for Illumina (NEBNext, NEB, USA). The random hexamer primer plus RNaseH-(M-MuLV Reverse Transcriptase ...
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bioRxiv - Plant Biology 2022Quote: ... The RNA-Seq paired end sequencing libraries were prepared from the quantity and quality check (QC) passed RNA samples using NEBNext® UltraTMII directional RNA Library Prep Kit for Illumina (NEB) as per manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing adaptors from the Oxford Nanopore Ligation Sequencing Kit (ONT, LSK109) were ligated to DNA ends using Quick Ligase (NEB, M2200) for 10 minutes at room temp ...
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bioRxiv - Molecular Biology 2023Quote: ... and libraries for next-generation sequencing were prepared using the NEBNext Ultra(tm) II Directional RNA Library Prep kit with Sample Purification Beads (NEB, E7765S). Libraries were indexed using NEBNext Multiplex Oligos for Illumina Index Primers Set 1 and 2 (E7335S and E7500S) ...
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bioRxiv - Microbiology 2022Quote: ... LAMP reactions were set up using the NEB WarmStart® LAMP Kit (DNA & RNA) (E1700S, New England Biolabs, Ipswich, Massachusetts, USA) and using the two designed primer sets ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA samples from empty vector and sgRNA2 MEFs were converted into sequencing libraries with the “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” (NEB #E7760), as recommended by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... The eluted DNA was used for sequencing library preparation with NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, E7645). Biotinylated DNA fragments were enriched by Dynabeads M-280 Streptavidin (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... at −80 °C prior to RNA extraction and library preparation with NEB Next® Ultra™ RNA Library Prep Kit (NEB) by Novogene ...
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bioRxiv - Plant Biology 2023Quote: Illumina library was prepared at GIGA Genomics (University of Liege, Belgium) using NEBNext Ultra II DNA library prep kit (New England BioLabs, US) and libraries were sequenced on the Illumina NextSeq500 sequencer for the generation 10 M of paired-end reads (2 x 150 base pair ...
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bioRxiv - Neuroscience 2022Quote: ... was used as a template and transcribed in vitro using HiScribe™ T7 ARCA mRNA Kit (with tailing) (New England Biolabs) following manufacturer protocol (E2060 ...
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bioRxiv - Microbiology 2023Quote: MNV plasmids were linearised with NotI and phenol/chloroform extracted before being used for in vitro transcription using the HiScribe™ T7 ARCA mRNA Kit (NEB), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Obtained DNA was used for library preparation with NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (NEB E7370L). Libraries were run on Novaseq 6000 (Illumina ...
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bioRxiv - Genetics 2022Quote: ... Between 0.5-1 μg of chromatin was proceeded to MeDIP (Methylated DNA Immunoprecipitation) according to the manufacture protocol (EpiMark® Methylated DNA Enrichment Kit, New England Biolabs). MeDIP DNA was quantified by qPCR with specific primers and sequencing.
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bioRxiv - Neuroscience 2023Quote: ... RNA-seq libraries were prepared with 10 ng of total RNA using NEBNext® single cell/low input RNA library preparation Kit (New England Biolabs). Single-end sequencing was performed on all the samples with a 75 bp sequencing depth on a NextSeq machine at the RNomics platform of the Université de Sherbrooke (Canada) ...
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bioRxiv - Neuroscience 2022Quote: ... and used to generate point mutations in the GlyT2 cDNA/pOTV construct utilising the Q5 site-directed mutagenesis kit (New England Biolabs (Genesearch), Arundel ...
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bioRxiv - Synthetic Biology 2022Quote: ... Purified DNA was eluted in 30 μl of Ultrapure water and 10ng was inputted into the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S) using the following PCR programme ...
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bioRxiv - Molecular Biology 2022Quote: ... and 500 ng of the gDNA was subjected to DNA library preparation using the NEBNext Ultra II FS DNA Library Prep kit for Illumina (New England Biolabs, E7805S) following manufacturer’s instructions (protocol for inputs >100 ng) ...
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bioRxiv - Neuroscience 2022Quote: ... Deletion mutant EGFP-Inka2ΔiBox and EGFP-Inka2ΔCC were constructed from pEGFP-Inka2 using Q5 Site-Directed Mutagenesis Kit (New England BioLabs, Ipswich, MA, USA). Expression plasmids for Pak1 (pCMV6M-Pak1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared following the instruction from NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645L) and indexes (NEB #7335S ...
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bioRxiv - Molecular Biology 2022Quote: ... Up to 10 ng of DNA was used for the library construction using NEBNext Ultra II DNA Library Prep Kit (NEB, E7645). Sequencing was performed on NextSeq 500 (Illumina).
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bioRxiv - Molecular Biology 2022Quote: ... One µg of RNA together with oligo(dT) primers were used for the cDNA synthesis using the ProtoScript®II first strand cDNA synthesis kit (New England Biolabs) according to the manufacture’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... About 75 ng of barcoded libraries were ligated to the AMII sequencing adaptors (Oxford Nanopore Technologies, UK) using the Quick ligation kit (New England Biolabs, UK), then finally purified and quantified with Ampure XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2022Quote: RLuc-P2A-X-P2A-Fluc plasmids (where X represents a variable sequence) were assembled using NEB Builder HiFi DNA Assembly Cloning Kit (New England Biolabs, #E2621S) by combining the original plasmid digested with HindIII (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: The tRNA-Pro coding sequence was ordered as gBlock (listed in Table S2) and was assembled with digested YCplac33 backbone using NEBuilder HiFi DNA Assembly Cloning Kit (NEB, #E5520). The tRNA-Pro probe sequence was amplified from this plasmid using the primers in Table S2 and in vitro transcribed by using Digoxigenin-11-UTP included in DIG Northern Starter Kit (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... The enrichment of the microbial DNA was performed using the NEBNext® Microbiome DNA Enrichment Kit (New England Biolabs Inc, MA) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2022Quote: The library for EM-seq was prepared using 200 ng DNA input and the NEBNext Enzymatic Methyl-seq Kit (NEB, E7120S) following the manufacturer’s instructions for a standard insert (370-420 bp) ...
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bioRxiv - Molecular Biology 2022Quote: ... following the manufacturer’s instructions and the recovered poly(A) selected RNA was used for cDNA library preparation using NEBNext Ultra II directional RNA library kit (NEB, E7760) following library preparation protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The final RNA concentration was determined using Qubit RNA high Sensitivity Assay Kit and 100ng of RIP-purified RNA was used for cDNA library preparation using NEBNext Ultra II directional RNA library kit (NEB, E7760) following library preparation protocol ...
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bioRxiv - Molecular Biology 2022Quote: The library for RNA-seq was prepared with 200 ng RNA input and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs (NEB), E7760L ...
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bioRxiv - Molecular Biology 2022Quote: ... Library preparation of immunoprecipitated DNA fragments was performed using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs: E7645) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... were generated by deletion of the unwanted DNA from the parental plasmid using the Q5 Site-Directed Mutagenesis Kit (NEB BioLabs). The R763G point mutation was introduced into D1-A1 by PCR mutagenesis ...
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bioRxiv - Developmental Biology 2022Quote: ... pUASTattB-bon-mCherry and pUASTattB-bon-Y507A Y585A-mCherry were constructed using NEBuilder HiFi DNA Assembly Cloning Kit (NEB, Cat# E5520S). All tags were added to the C-terminus of the genes.
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bioRxiv - Plant Biology 2022Quote: ... treatment and used for complementary DNA (cDNA) synthesis with a ProtoScript® II First Strand cDNA Synthesis Kit (New England BioLabs). To clone the coding region of maize BABY BOOM (BBM ...