Labshake search
Citations for New England Biolabs :
8101 - 8150 of 9501 citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... pUASTattB-bon-mCherry and pUASTattB-bon-Y507A Y585A-mCherry were constructed using NEBuilder HiFi DNA Assembly Cloning Kit (NEB, Cat# E5520S). All tags were added to the C-terminus of the genes.
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bioRxiv - Plant Biology 2022Quote: ... treatment and used for complementary DNA (cDNA) synthesis with a ProtoScript® II First Strand cDNA Synthesis Kit (New England BioLabs). To clone the coding region of maize BABY BOOM (BBM ...
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bioRxiv - Immunology 2022Quote: ... The resulting fragment was then incorporated to NcoI-digested pMSCV-IRES-mCherry using the Gibson Assembly Cloning Kit (New England Biolabs, E5510S). The construct was transformed into E ...
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bioRxiv - Neuroscience 2022Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina using the manufacturer’s instructions (NEB, Ipswich, US). Briefly ...
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bioRxiv - Plant Biology 2022Quote: ... and libraries were prepared using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, Ipswish MA, USA). Libraries were then quantified using Qubit™ Fluorometer 4 and Fragment analyzer 5200 (Agilent ...
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bioRxiv - Molecular Biology 2022Quote: ... Two to three PCR reactions per sample were combined and cleaned-up using the Monarch PCR and DNA clean-up kit (NEB, T1030S). 10-100 ng of DNA from each sample was carried forward for end-repair using the NEBNext Ultra II End repair/dA-tailing Module (NEB ...
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bioRxiv - Bioengineering 2022Quote: ... RNA-seq libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, Cat. No. / ID: E7770) following the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2022Quote: ... using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs Inc, Ipswich, Massachusetts, USA), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Barcoded stranded mRNA-seq libraries were prepared from 300 ng of high-quality total RNA samples using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries for ribosome profiling were prepared following the NEBNext Multiplex Small RNA Library Prep kit for Illumina’s protocol (NEB, Cat# E7300S). Ribo-seq ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries for small DNA fragments (25-75 bp) were prepared based on the NEBNext Ultra II DNA library prep Kit for Illumina (NEB#E7645).
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bioRxiv - Microbiology 2023Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, NEB) using 200-600ng total RNA as input ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting amplicon was used as starting material for in vitro transcription using HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S). Finally ...
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bioRxiv - Bioengineering 2024Quote: cDNA of each library was generated using oAS345 (Supplemtary Note 21) and LunaScript Primer-Free RT Master Mix Kit (NEB E3025S).
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bioRxiv - Bioengineering 2024Quote: ... The bacterial pellet was then resuspended in 1X DNA/RNA protection reagent supplied with the Monarch Total RNA Miniprep Kit (NEB #T2010) (New England BioLabs, USA) and subjected to mechanical lysis using the FastPrep-24™ 5G bead beating grinder system (MP Biomedicals Germany GmbH) ...
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bioRxiv - Bioengineering 2024Quote: ... The bacterial pellet was then resuspended in 1X DNA/RNA protection reagent supplied with the Monarch Total RNA Miniprep Kit (NEB #T2010) (New England BioLabs ...
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bioRxiv - Cell Biology 2024Quote: ChIP-seq libraries were prepared independently from two ChIP biological replicates using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, #E6177L) according to manufacturer’s instructions with a few modifications ...
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bioRxiv - Bioengineering 2024Quote: High Molecular Weight (HMW) DNA was obtained using the Monarch® HMW DNA extraction kit for Tissue (T3060L, New England Biolabs), as previously described (Low et al ...
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bioRxiv - Molecular Biology 2024Quote: ... libraries were constructed with the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (catalog no. E7760L, NEB) and indexed with NEBNext® Multiplex Oligos for Illumina (catalog no ...
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bioRxiv - Cell Biology 2024Quote: ... The amplified fragment containing SNAP was assembled with an amplified pUbq destination backbone using a NEBuilder® HiFi DNA Assembly kit (NEB) to create a pUbq-SNAP destination vector ...
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bioRxiv - Cell Biology 2024Quote: ... The pRNA destination backbone was linearised by primers s5 and s6 (Table 3) and assembled with the mScarlet-I3 fragment using a NEBuilder® HiFi DNA Assembly kit (NEB) to create a pRNA-mScarlet-I3 destination vector ...
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bioRxiv - Microbiology 2023Quote: ... following the manufacturer’s protocol and cDNA library preparation using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Massachusetts, USA). DNA library quality control was done by measuring concentration using the Qubit 2.0 fluorometer (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: DNA was extracted from lentiviral transduced cells using the Monarch® Genomic DNA Purification Kit (New England Biolabs, Ipswich, MA, USA), and its quality was determined using the NanoDrop Q500 spectrophotometer (Quawell CF ...
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bioRxiv - Biochemistry 2023Quote: jAspSnFR3 and jAspSnFR3-mRuby3 were first cloned into entry vector pENTR1A (Fisher, A10462) using NEBuilder HiFI DNA Assembly Cloning Kit (New England BioLabs, E2621). These donor constructs were then used to transfer their insert into destination vectors ...
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bioRxiv - Physiology 2023Quote: ... were PCR-amplified from wild-type N2 gDNA and subcloned into the SpeI and NheI sites of a pL4440 expression vector with NEBuilder® HiFi DNA Assembly Cloning Kit (E2621L, NEB). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... library indexing was carried out with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Cat. E7645S) and sequenced on a NextSeq 1000 P2 cartridge (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... These gene fragments were cloned into pcDNA3.1-PsChR2-eYFP backbone vector using the HiFi Assembly Kit (New England Biolabs, lpswich, MA).
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bioRxiv - Cancer Biology 2024Quote: ... Whole exome library construction was generated using the NEB Next® Ultra RNA Library Prep Kit for Illumina (E7530L, NEB, USA) after DNA shearing ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA-sequencing libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep kit for Illumina® (NEB) following supplier’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 ng of purified CUT&RUN enriched DNA was used to prepare Illumina library using the NEBNext Ultra II DNA Library Prep kit (NEB, #E76450) per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, no. E7775) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... gcry1:BFP vector backbone was assembled with the 2A and nCas9n PCR amplicons using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB # E5520S) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... and 100 ng of RNA was reverse-transcribed into cDNA using the LunaScript RT Master Mix Kit (New England Biolabs #E3025L). qPCR reactions utilized specific primers and were performed in triplicate with Applied Biosystems TaqMan Gene Expression Master Mix (ThermoFisher #4369016) ...
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bioRxiv - Microbiology 2024Quote: ... RT-qPCR reactions were carried out in 10 μL reactions using the Luna® One-Step Universal RT-qPCR kit (NEB) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: A spike-in Luciferase RNA was first transcribed in vitro using HiScribe™ T7 Quick High Yield RNA Synthesis Kit (New England Biolabs) according to Manufacturer’s instructions ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... RNA library preparation was performed using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB #E7775) or the SMRTbell Prep Kit 3.0 for Illumina and Iso-Seq respectively ...
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bioRxiv - Neuroscience 2024Quote: ... and prepared using NEBNext Ultra II Directional RNA Library Prep Kit with Sample Purification Beads (E7765; New England Biolabs, Ipswitch, MA). cDNA libraries were then indexed using the NEBNext Dual Index Kit (E7600S ...
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bioRxiv - Molecular Biology 2024Quote: ... ABCA4 missense variants were introduced in WT-ABCA4-SmBiT by site-directed mutagenesis using Q5 Site-directed mutagenesis kit quick protocol (NEB, E0554) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: RNA-seq libraries were prepared from 100 ng RNA/sample using the NEBNext Ultra II RNA library preparation kit for Illumina (NEB E7770S) in conjunction with the NEBNext poly(A ...
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bioRxiv - Molecular Biology 2024Quote: ... and 50 ng of material was fragmented for 8 min at 37 °C and A-tailed for 30 min at 65 °C using NEBNext Ultra II FS DNA Library Prep Kit (NEB, E7805S). The fragmented and dA-tailed DNA library was ligated to dsDNA adapter having both forward (Ligation FWD primer ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of PCR product was used in an in vitro transcription reaction using the HiScribe T7 High Yield RNA Synthesis kit (NEB, E2040S) in the presence of 20units/mL SUPERaseIn ...
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bioRxiv - Molecular Biology 2024Quote: ... was added to 500 ng total RNA then the rRNA was depleted with the NEBNext rRNA depletion kit V2 (NEB, E7400L) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were barcoded using Illumina i5 and i7 adaptors from NEBNext® Multiplex Oligos for Illumina (Dual Index Primers Set 1) kit (New England Biolabs) and sequenced using a 150-cycle NextSeq 500/550 High Output Kit v2.5 with 75 forward and 75 reverse reads ...
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bioRxiv - Neuroscience 2024Quote: ... C terminal and αPKC binding region) or PICK1 (BAR domain) were made with NEBuilder HiFi DNA Assembly Cloning Kit (NEB #E5520S). Mutations of PICK1 (KD-AA and 5K-E ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Libraries were created using 1000ng of total RNA with the NEBNext Ultra 2 Directional RNA Library Kit following the Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) and sequenced on an Illumina Hi-Seq 2000 ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was prepared with 0.1-1 µg of total RNA using the Protoscript First Strand cDNA Synthesis kit (New England Biolabs; Catalogue #E6560L) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was extracted from zebra finch and chicken embryos using QIAgen RNeasy kit and transcribed into cDNA using LunaScript RT (NEB #E3010). PCR was performed using chicken or zebra finch cDNA and gene specific primers (Supplemental table 33) ...
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bioRxiv - Cell Biology 2024Quote: ... The WT PACT expression vector was used as template for constructing p38 mutant PACT clone by using Q5 site-directed mutagenesis kit (New England Biolabs, E0554S). Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Qualified RNA samples were subjected to sequencing library construction using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, E7775). Paired-end (2 × 150 bp ...
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bioRxiv - Genomics 2023Quote: ... DNA libraries for Next Generation Sequencing were prepared with NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs E7775) and NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors RNA Set 1 ...