Labshake search
Citations for New England Biolabs :
7751 - 7800 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 1 μl of the diluted cDNA was used in 10 μl reaction mixture of Q5 Hot START DNA Polymerase kit (NEB) (2 μl of 5X buffer ...
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bioRxiv - Neuroscience 2020Quote: ... mutations were inserted into the open reading frame of Vesicular stomatitis virus glycoprotein VSV-G using the Q5 SDM kit (NEB). Myc epitope-tagged SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Microbiology 2020Quote: ... column purified (QIAquick PCR Purification kit) and ligated with restriction enzyme cut-pHH21 using T4-ligase (New England Biolabs, NEB). Ligation products were transformed into DH5a (NEB ...
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bioRxiv - Microbiology 2020Quote: ... column purified (QIAquick PCR Purification kit) and ligated with restriction enzyme cut-pHH21 using T4-ligase (New England Biolabs, NEB). Ligation products were transformed into DH5a (NEB ...
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bioRxiv - Microbiology 2021Quote: ... the codon optimized RBD was PCR amplified and cloned into the bicistronic pDZ rescue plasmid system for A/Puerto Rico/8/1934 using the NEBuider HiFi DNA assembly kit (NEB). Specifically ...
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bioRxiv - Genomics 2020Quote: ... The first step involved incubation of total DNA with proteinA-MBD2-Fc mixture (NEBNext Microbiome DNA Enrichment Kit, New England BioLabs, Microbial enrichment kit ...
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bioRxiv - Genomics 2020Quote: ... Samples were prepared for Illumina sequencing with NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (NEB #E7775) accompanied by NEBNext Poly(A ...
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bioRxiv - Genomics 2021Quote: ... C and 5mC deamination reaction was performed using the APOBEC3A enzyme (NEBNext® Enzymatic Methyl-seq Kit, New England Biolabs) with the following ramping conditions ...
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bioRxiv - Genetics 2021Quote: ... Repair templates were generated by assembling homology arms (450-bp gBlocks synthesized by IDT) and the selection cassette using the NEBuilder Hifi DNA Assembly Kit (New England Biolabs).
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bioRxiv - Microbiology 2021Quote: ... cDNA was amplified and indexed using the PCR Barcoding Kit (Oxford Nanopore Technologies) and LongAmp Taq 2X master mix (New England Biolabs). The resulting cDNA libraries were sequenced on a MinION (MIN101B ...
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bioRxiv - Microbiology 2021Quote: ... and sequencing libraries were generated using the rRNA-depleted RNA samples using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... pcineo NS1 single site mutants in the full-length construct were generated by using the Q5 site-directed mutagenesis kit (New England BioLabs), following the manufacturer recommendations ...
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Lessons from the meiotic recombination landscape of the ZMM deficient budding yeast Lachancea waltiibioRxiv - Genetics 2021Quote: ... DNA libraries were prepared from 5 ng of total genomic DNA using the NEBNext Ultra II FS DNA Library kit for Illumina (New England Biolabs). All volumes specified in the manufacturer’s protocol were divided by four ...
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bioRxiv - Microbiology 2021Quote: ... was digested with NotI and NheI and the mgrB PCR product was cloned using the NEBuilder HiFi DNA assembly kit (NEB), followed by transformation into the E ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 RNA levels were quantified with 250 ng of RNA inputs using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs), using real-time RT-PCR primer/probe sets 2019-nCoV_N1 (CDCN1 ...
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bioRxiv - Immunology 2021Quote: ... pSFG-SARS-CoV-2 S D614G was cloned from pSFG-SARS-CoV-2 S plasmid using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). 293T cells were transfected with 3.75 μg pSFG-SARS-CoV-2 S or pSFG-SARS-CoV-2 S D614G ...
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bioRxiv - Immunology 2021Quote: ... Point mutations and deletions of cGAS were constructed using a Q5® Site-Directed Mutagenesis Kit (New England Biolabs, # E0554S).
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bioRxiv - Immunology 2021Quote: ... The resultant DNA was cloned into a pUC19 plasmid and in vitro transcribed using a HiScribe T7 High Yield RNA synthesis kit (New England Biolabs). After DNAseI digestion and clean-up with a TURBO DNA-free kit (Thermo-Fisher) ...
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bioRxiv - Immunology 2021Quote: ... the pCMV3-SΔ19 insert was initially digested with KpnI and blunt polished using Phusion Taq polymerase followed by a DNA cleanup using the Monarch PCR cleanup kit (NEB) and a second digest was done using NotI ...
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bioRxiv - Neuroscience 2020Quote: ... Sequencing libraries were created using the NEBNext Ultra II DNA Library Prep kit for Illumina sequencing (New England Biolabs E7645L). All cDNA and library clean-up was done using SPRIselect beads (Beckman Coulter B23317).
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bioRxiv - Genomics 2021Quote: ... A reporter containing the alternate allele was generated through SDM using the Q5 Site-Directed Mutagenesis kit (New England Biolabs). The primer sequences used were as follows:
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bioRxiv - Genomics 2021Quote: ... Libraries were prepared using NEBNext Poly(A) mRNA magnetic isolation module and NEB Ultra Directional RNA Library kit for Illumina (New England Biolabs).
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bioRxiv - Microbiology 2020Quote: ... A bacteria-codon optimized gBlock for the truncated protein was produced by IDT DNA Technologies and cloned into a pET28a bacterial expression with a C-terminal 6xHis tag using the NEBuilder Assembly Kit (New England Biolabs). Recombinant protein was expressed in BL21(DE3 ...
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bioRxiv - Microbiology 2020Quote: ... The LPMO and CBH1 amplicons for detection were radio-labelled with [α-32P] dCTP using the NEBlot Kit (NEB, USA) according to the manufacturer’s instructions and used as a probe to determine the copy number of T-DNA integrations in all the transformants.
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bioRxiv - Microbiology 2020Quote: DNA was extracted from high-titer phage lysates and sequencing libraries were prepared using the Ultra II FS Kit (New England Biolabs) or for ARB14 and ARB25 ...
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bioRxiv - Molecular Biology 2020Quote: Many of the reagents used in 5XP-seq are included in the NEBNext Multiplex Small RNA Library prep kit for Illumina (New England Biolabs), and are referred to as ‘NEB-kit’ below ...
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bioRxiv - Microbiology 2021Quote: ... VN173 fused to NiV-M was generated by exchanging Ub from the previously described VN173-Ub (Pentecost et al., 2015) for NiV-M using the Gibson Assembly Cloning Kit (New England BioLabs). MeV-M BiFC constructs were made by exchanging NiV-M fused to VN173 or VC155 for MeV-M using the Gibson Assembly Cloning Kit (New England BioLabs) ...
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bioRxiv - Microbiology 2021Quote: ... All mutant plasmids were generated using the Q5 site-directed mutagenesis kit using PAGE-purified mutagenesis primers designed using the online NEBase Changer primer design tool (New England BioLabs). The mCherry plasmid was created by cloning mCherry into the multiple cloning site of pTriEx-3 (Novagen ...
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bioRxiv - Microbiology 2021Quote: ... MeV-M BiFC constructs were made by exchanging NiV-M fused to VN173 or VC155 for MeV-M using the Gibson Assembly Cloning Kit (New England BioLabs). All mutant plasmids were generated using the Q5 site-directed mutagenesis kit using PAGE-purified mutagenesis primers designed using the online NEBase Changer primer design tool (New England BioLabs) ...
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bioRxiv - Microbiology 2021Quote: ... Genomic libraries were constructed and barcoded using the NEBNext Ultra DNA Library Prep kit (New England Biolabs, Ipswich, MA, USA) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... were cloned into the pEX18.AP vector (with ampicillin resistance as a selectable marker) using the Gibson Assembly Cloning Kit (New England Biolabs) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... PCR was carried out in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with SARS-CoV-2 NP-specific primers (Forward 5’-TAA TCA GAC AAG GAA CTG ATT A-3’ ...
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bioRxiv - Neuroscience 2019Quote: ... The R1320P mutation was created in a wild-type cDNA using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Wild-type and R1320P mutant dNf1 were then subcloned into the pUAST-attB vector with an in-frame C-terminal fusion with eGFP cDNA ...
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bioRxiv - Microbiology 2021Quote: ... and barcoded using an Native Barcoding Expansion 96 kit (Oxford Nanopore Technologies) and a Blunt/TA Ligation Master Mix (New England Biolabs). Barcoded samples (96 ...
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bioRxiv - Microbiology 2021Quote: ... 4μL purified RNA were subsequently used for RT-qPCR reaction using the Luna Universal One-Step RT-qPCR kit (New England Biolabs) and 0.4μM of each primer ...
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bioRxiv - Microbiology 2021Quote: ... Brunello and Vervet DNA samples were amplified in ten 100 μl reactions using the NEBNext High-Fidelity 2X Master Mix Kit (New England Biolabs), 0.5 μM of forward and reverse primers and 10 μg of DNA template per reaction with the following program ...
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bioRxiv - Microbiology 2021Quote: ... The SARS-CoV-2 S R403T and RaTG13 S T403R/T403A mutant plasmids were generated using Q5 Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Plant Biology 2021Quote: Total RNA was used to make sequencing libraries using a NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... the DNA of the two parents that generated the cross was extracted from flash-frozen young leaves using NEBNext Ultra II kit (NEB) and sequenced to ~40x coverage as 150 bp reads on an Illumina MiSeq at the Biological Nanostructures Lab in the California NanoSystem Institute at UC Santa Barbara ...
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bioRxiv - Plant Biology 2021Quote: ... RNA-Seq libraries were prepared from 1μg high quality RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs), according to the manufacturer’s guidelines ...
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bioRxiv - Plant Biology 2021Quote: ... PCR product of the expected size was purified from the agarose gel after electrophoresis using the Monarch DNA Gel Extraction Kit (NEB), and cloned into the pDONR207 vector (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... and Fls3 clones was performed in the entry vectors using the Q5 Site-Directed Mutagenesis kit following the manufacturer’s instructions (New England Biolabs, www.neb.com). For a list of all primers and constructs used in this study ...
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bioRxiv - Microbiology 2021Quote: ... The product was then transcribed to crRNA using HiScribe T7 Quick High Yield RNA Synthesis kit (New England Biolabs; E2050S) incubating at 37°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... pDWV-S-GFP and the parental pDWV-304 were produced using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... RNA was subjected to OneStep qRT-PCR analysis using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) and a CFX96 Real-Time System ...
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bioRxiv - Molecular Biology 2021Quote: ... with primers carrying the appropriate restriction enzymes sites AscI/SbfI (See Table S6 for the list of primers used) and cloned using Quick DNA Ligation Kit (NEB) into dCas9-empty-GFP vector ...
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bioRxiv - Molecular Biology 2021Quote: ... All cDNA libraries were constructed according to the standard protocol of the NEBNext Ultra RNA Library Prep Kit for Illumina (New England BioLabs). The synthesized double-stranded cDNA was end-repaired using NEBNext End Prep Enzyme Mix before ligation with NEBNext Adaptor for Illumina ...
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bioRxiv - Microbiology 2020Quote: ... The mRNA libraries were generated using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760L, New England Biolabs) and NEBNext Poly(A ...
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bioRxiv - Microbiology 2020Quote: ... Linkers (5’-ACTAGTTCCGAGCTCGAG-3’) with restriction sites for SpeI and XhoI were introduced by PCR based mutagenesis using the Q5 mutagensis kit (NEB) after codon 466 of nsP2 (2EGFP ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: Plasmid derivatives of pKR72 with pho-lac fusions were constructed in frame with different codons of YegI using the Q5 Site-Directed mutagenesis kit (NEB). Primers used to generate pho-lac fusions in frame to different codons E439 (KR230/KR231) ...