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Citations for New England Biolabs :
7151 - 7200 of 9547 citations for QuantiChrom Phosphate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Poly(A) mRNA library preparation was performed using NEBNext Ultra II DNA library prep kit for illumina (New England BioLabs) and 2x100bp paired-end sequencing was performed at a depth of 50 million reads on an Illumina Novaseq 6000 platform by the University of Toronto Donelly Sequencing Centre ...
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bioRxiv - Genomics 2024Quote: ... and reverse-stranded sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). Libraries were sequenced on an Illumina NovaSeq 6000 machine ...
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bioRxiv - Evolutionary Biology 2024Quote: RNA was extracted from heads and thorax+abdomen of one female and one male using the Monarch Total RNA Miniprep Kit (New England, BioLabs). RNA was purified by ethanol precipitation and equal concentration of head and thorax+abdomen tissue was pooled for sequencing ...
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bioRxiv - Evolutionary Biology 2024Quote: RNA libraries were constructed using the NEBNext Ultra II RNA Library prep for Illumina kit (New England Biolabs, MA, USA), NEBNext Poly(A ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids containing either rpsL-cat or the inducible promoter tetO1 [18] flanked by regions homologous to the DNA up- and downstream of the ggt-promoter were constructed using a commercially available Gibson Assembly Cloning Kit (NEB). Homologous regions were amplified from the genomic DNA of strain PMSS1 using primer pairs FlgK fwd/FlgK rev (rpsL/tetO1 ...
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bioRxiv - Immunology 2024Quote: ... mRNA used for comparison in NAGE gels was produced using a HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The PCR amplicon was purified by sequential gel extraction (1.5% agarose gel prepared in 0.5x TAE) and affinity column chromatography (Monarch® PCR & DNA Cleanup Kit, New England Biolabs). For IVT synthesis ...
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bioRxiv - Immunology 2024Quote: ... Template DNA digestion was carried out at 37 °C for 30 minutes in a thermocycler block prior to purification of the RNA using a Monarch RNA Cleanup Kit (New England Biolabs). Purified mRNA was eluted in DEPC-treated water and stored at -80 °C prior to use ...
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bioRxiv - Genomics 2024Quote: ... Library amplification (10-12 PCR cycles) was carried out using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs), with IDT for Illumina UD Indexes (96x ...
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Recurrent loss of crossover interference punctuates the recombination landscape across yeast speciesbioRxiv - Genomics 2024Quote: DNA libraries were prepared from 5 ng of total genomic DNA using the NEBNext Ultra II FS DNA Library kit for Illumina (New England Biolabs). All volumes specified in the manufacturer’s protocol were divided by four ...
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bioRxiv - Genetics 2024Quote: In vitro transcription was carried out on a linear DNA template using the HighScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Libraries were then created from each fraction using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645L) and the Unique Dual Index NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Microbiology 2024Quote: ... L742A and L786A mutations were inserted into pACEBac1-Strep-Psc-BICD2711-800 (either individually or in combination) by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (NEB). The full-length mouse BICD1 sequence (UniProt Q8BR07 ...
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bioRxiv - Microbiology 2024Quote: ... AnTat1.1 sequence was obtained from AnTat1.1 specific cDNA from mouse infection D6 cloned into a pMiniT vector with the PCR Cloning Kit (NEB, E1202S). VSG-228 was partially amplified from VSG PCR (see below ...
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bioRxiv - Microbiology 2024Quote: ... reactions were performed in a final volume of 100 μl using the PURExpress ΔRibosome In vitro Protein Synthesis Kit (New England Biolabs) according to the manufacturer instructions and with ribosomes purified from E ...
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bioRxiv - Immunology 2024Quote: ... Libraries were prepared with 5ng of CUT&RUN DNA using NEBNext Ultra II DNA Library Prep Kit for Illumina (Cat # E7645S, NEB) following manufacturers protocol ...
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bioRxiv - Immunology 2024Quote: VR therapy vectors and the CAR therapy vector were first designed through Gibson Assembly molecular cloning (HiFi DNA Assembly Cloning Kit, NEB) using the backbone pSLCAR-CD19-BBz (Addgene) ...
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bioRxiv - Microbiology 2024Quote: ... Libraries for Illumina sequencing were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) following manufacturer instructions ...
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bioRxiv - Genomics 2024Quote: ... Libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (New Englands Biolabs), multiplexed ...
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bioRxiv - Genomics 2024Quote: Between 2 and 4 million cells were harvested by centrifugation and high molecular weight (HMW) genomic DNA was extracted using the Monarch High Molecular Weight DNA Extraction kit (NEB) and agitation speeds of 1,500 rpm.
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bioRxiv - Immunology 2024Quote: ... The unpurified DNA product was then subjected to in vitro transcription using the NEB HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs), incubating at 37 °C for 16 h ...
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bioRxiv - Immunology 2024Quote: ... DNA libraries were then prepared for sequencing using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, #E7645S) and sequencing adaptors were ligated using NEBNext Multiplex Oligos for Illumina (#E7500S ...
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bioRxiv - Cell Biology 2024Quote: ... or 3 * 106 cells with the lowest or highest 5% of Spry4:H2B-Venus fluorescence were FAC sorted and their DNA isolated by column-based genomic DNA purification (Monarch Genomic DNA Purification Kit, NEB). For reference ...
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bioRxiv - Cell Biology 2023Quote: ... The sequences were cloned into the pUASz1.0 vector (54) with the eGFP ORF at the C-terminal end of the Top3β ORF using a ligation kit (NEB). The UASz vector allows one to induce the constructs in any tissue ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR product was purified by phenol chloroform extraction and RNA was synthesized using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB). 1 ug of starting linear DNA template was used and transcription was performed for 2hrs at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR-amplified DNA templates encoding 5’UTR of ATF4 mRNA were used for in vitro transcription of ATF4 reporter mRNA using HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2024Quote: ... site-directed mutagenesis of the codon encoding lysine at amino acid position 41 was altered to alanine using the Q5 site-directed mutagenesis kit (New England Biolabs) using pPM11 as a template ...
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bioRxiv - Cancer Biology 2024Quote: ... Purified ChIP DNA was used to prepare Illumina multiplexed sequencing libraries using the NEBNext Ultra II DNA Library Prep kit and the NEBNext Multiplex Oligos for Illumina (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... The 3xFLAG- and HA-tagged ZNRF3 expression vectors were generated with the Q5 Hot Start High-Fidelity DNA Polymerase Kit (New England Biolabs) by replacing the FLAG-tag ...
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bioRxiv - Cancer Biology 2024Quote: ... before ligation to microsatellite expansion adapters containing an EcoRV restriction site (listed in Supplemental Table 1) using the T4 DNA Ligase Kit (NEB) at a 5:1 ratio overnight at 16°C ...
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bioRxiv - Cell Biology 2024Quote: ... and inserted into BamHI-HindIII-digested p413-PGPD vector using the NEBuilder HiFi DNA Assembly kit (New England Biolabs, E5520S).
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bioRxiv - Cell Biology 2024Quote: ... Synthetic oligonucleotides encoding 3xFLAG were then ligated to the 5’ position of APEX2 sequence using NEBuilder HiFi DNA assembly kit (New England Biolabs) to form the final vector pcDNA5/FRT/TO/3xFLAG-APEX2.
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bioRxiv - Cancer Biology 2024Quote: ... and a final concentration determination was performed using NEBNext® Library Quant Kit for Illumina (New England Biolabs Cat. E7630) prior to library sequencing.
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bioRxiv - Cell Biology 2024Quote: ... or (Q71L for Arf1) and dominant negative (T27N for Arf6) or (T31N for Arf1) by using site-directed mutagenesis (SDM kit from NEB) kit ...
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bioRxiv - Cell Biology 2024Quote: ... Poly(A) RNA libraries were constructed using NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs, #E7770) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, NEB) using 200-600ng total RNA as input ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared on beads using the Next Ultra II DNA Library Prep Kit for Illumina and Next Multiplex Oligos for Illumina (New England Biolabs) following instructions from the Arima-HiC+ for HiC (Arima Genomics) ...
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bioRxiv - Molecular Biology 2024Quote: ... 10ng of purified DNA (average size 250–300bp) was used to prepare sequencing libraries with the Next Ultra II Library Prep Kit for Illumina (New England Biolabs) using the application note for “Low input ChIP-seq” ...
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bioRxiv - Molecular Biology 2023Quote: ... Resulting footprints were then used to generate sequencing libraries using the NEBNext Small RNA Library Prep Set for Illumina kit (New England BioLabs). Library quality was assessed using High Sensitivity DNA Chips with the Agilent 2100 Bioanalyzer System (Agilent Technologies).
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bioRxiv - Biophysics 2023Quote: ... The TRAK1 construct was made through assembling His-ZZ-TEV-SNAP tag and TRAK1(1-395) into a pACEBac1 vector via HIFI DNA assembly kit (NEB). All constructs were verified using whole plasmid sequencing from Plasmidsaurus.
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bioRxiv - Systems Biology 2023Quote: ... Whole metagenome sequencing libraries were prepared from 26 µL of DNA solution using the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs). The DNA was purified and size selected to remove excess adaptors and adaptor dimers using Ampure XP beads (Beckman Coulter Life Sciences) ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were run on a 1% agarose gel and DNA was extracted using Monarch DNA Gel Extraction Kit (New England Biolabs). DNA was sequenced by Sanger sequencing using either the forward or reverse primer ...
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bioRxiv - Immunology 2023Quote: ... Live CD19+ B cells and CD4+PD1+CXCR5- Tph cells were purified by high-speed sorting (FACSAria II) and were directly processed using NEBNext Single Cell/Low Input RNA Library Prep Kit (New England Biolabs), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... mutations were created in the chromoshadow domain of HP1γ in pGEMT-KpnI-HP1γ using the Q5 site-directed mutagenesis kit (New England Biolabs) according to manufacturer’s instructions (Data S1) ...
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bioRxiv - Systems Biology 2023Quote: ... and total RNA libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs), using 700ng of RNA per sample ...
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bioRxiv - Microbiology 2023Quote: ... Cloning was performed by assembling the purified PCR fragments into the specified pET derivative expression vector using the commercially available NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: ... A stop codon was inserted after amino acid residue 370 by site directed mutagenesis using Q5 Site-Directed Mutagenesis Kit (NEB), in order to express the truncated N1-370 construct ...
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bioRxiv - Genomics 2023Quote: ... 170-200 ng of library was indexed using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). The manufacturer’s protocol was followed with the following deviations ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNA from single adult male flies was extracted using using the Monarch Genomic DNA Purification Kit (New England Biolabs), using a protocol we developed (dx.doi.org/10.17504/protocols.io.bp2l694qklqe/ v1 ...
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bioRxiv - Cell Biology 2023Quote: ... 500ng of total RNA were used for RNA-Seq library preparation with the NEBNext Ultra II RNA Library Prep Illumina Kit (NEB) and sequenced with the NextSeq 500/550 sequencing platform (performed at the NYUAD Sequencing Center within the NYUAD Core Technology Platform) ...