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Citations for New England Biolabs :
7351 - 7400 of 9547 citations for QuantiChrom Phosphate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Subsequent steps of RNA-seq were outsourced to Azenta who generated sequencing libraries from polyA-enriched RNA (captured with oligo-dT beads) using the NEBNext Ultra II RNA library prep kit for Illumina (NEB), multiplexed them ...
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bioRxiv - Developmental Biology 2023Quote: ... Library preparation was either carried out using 0.5 μg of RNA with NEBNext Ultra RNA library Prep Kit for Illumina (New England BioLabs, E7530L) or was performed by Novogene Services ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were constructed from ∼2ng DNA and prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB). Library quality was assessed by Fragment Analyzer NGS ...
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bioRxiv - Developmental Biology 2023Quote: ... Library preparation for high-throughput sequencing was performed on 100 ng of rRNA depleted RNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Sequencing was done with a NextSeq 500/550 High Output Kit v2.5 (150 Cycles) ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA-seq libraries were constructed by NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB #E7775). Briefly ...
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bioRxiv - Genomics 2023Quote: ... was used to prepare libraries with the NEBNext Ultra II library preparation kit with unique dual index primers (New England Biolabs). The library quality and quantity were verified by BioAnalyzer DNA 1000 (Agilent ...
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bioRxiv - Genetics 2023Quote: ... RNA was prepared for sequencing using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760). Sequencing was performed on an Ilumina NovaSeq 6000 instrument equipped with an S4 flow cell generating 150bp paired-end reads ...
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bioRxiv - Cell Biology 2023Quote: ... The two PCR-amplified DNA blocks were inserted into the linearized AAV-TBG vector by Gibson Assembly using an NEBuilder HiFi DNA assembly kit (NEB) following the manufacturer’s instruction.
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bioRxiv - Cell Biology 2023Quote: pTO-GFP-SNX17 was used to generate an shRNA resistant version of GFP-SNX17 by introducing three silent mutations into the target sequence of the SNX17 shRNA clone 1 construct using the Q5-site directed mutagenesis kit (New England Biolabs). Specifically ...
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bioRxiv - Cell Biology 2023Quote: Poly(A)+ RNA was isolated from nuclei and whole cells (see C2C12 cell fractionation above) using the Magnetic mRNA Isolation Kit (New England Biolabs). Two rounds of binding ...
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bioRxiv - Biochemistry 2023Quote: ... Parasites were collected by centrifugation (4000 xg, 10 min) and mRNA was extracted using a Quick-RNA Miniprep kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... A 200nt polyA tail was added through PCR and in vitro transcription was carried out using the HiScribe T7 High Yield RNA Synthesis kit (NEB). An m7G cap was introduced by using Vaccinia capping enzyme (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... SPI plasmids were generated by gap-repair cloning directly in yeast or by using the NEBuilder HiFi assembly kit (New England Biolabs) by combining linearised plasmid with gene fragments with homologous ends ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760S/L) following the kit protocol ...
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bioRxiv - Biochemistry 2023Quote: ... 5′ FAM-ACCCCGCATTACGTTTGGTGGACC-BHQ1 3′) and the Luna Universal Probe one-step RT-qPCR kit (catalog no. E3006; New England Biolabs). A 20-μL RT-qPCR mixture contained 7 μL of sample ...
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bioRxiv - Biochemistry 2023Quote: ... Around 1μg of DNA was isolated from each reaction using a Monarch PCR & DNA Cleanup Kit (elution volume of 15 μl, NEB). The DNA was treated with 5 U of Antarctic Phosphatase (NEB #M0289S ...
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bioRxiv - Genetics 2023Quote: ... The sheared DNA was then used as a template for libraries prepared with a NEBNext Enzymatic Methyl-seq Kit (EM-seq) (New England Biolabs). We note that our previous attempts to develop a hybridization capture protocol using bisulfite-converted DNA were unsuccessful ...
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bioRxiv - Cancer Biology 2022Quote: ... and then ligating annealed sgRNAs and pre-digested and purified LentiCRISPRv2E vector with Quick Ligation Kit (New England Biolabs, #M2200S). Plasmids were verified to contain expected sgRNA sequences by colony PCR (using LKO.1 5’ and pLentiCRISPR-R1 primers ...
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bioRxiv - Microbiology 2023Quote: ... 50 ng of total RNA was used as input into the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs). Libraries were prepared following the manufacturer’s protocol for use of the kit with purified mRNA or rRNA-depleted RNA ...
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bioRxiv - Molecular Biology 2023Quote: ... Double-stranded DNA was then entered into the end-repair module of RNA Library Prep Kit for Illumina from NEB, and size selected for 500-700 bp inserts using AMPure XP beads ...
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bioRxiv - Immunology 2023Quote: RNA-seq libraries were generated following the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB #E6420S) protocol for cells ...
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bioRxiv - Genomics 2023Quote: ... The fragmentation pattern was assessed via agarose gel electrophoresis and 0.5-1 μg of fragmented gDNA was used to prepare sequencing libraries using the NEBNext Ultra II DNA Library Preparation Kit (New England Biolabs), according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and P2 adaptor ligation steps were performed using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs). After size selection using AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Bioengineering 2023Quote: RT-qPCR was carried out from the isolated RNA using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) following the manufacturer instructions ...
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bioRxiv - Physiology 2023Quote: ... The remaining steps of library preparation were done using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). Adapters and PCR primers were purchased from New England BioLabs.
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bioRxiv - Physiology 2023Quote: ... between 400 and 3000 pg of total RNA were used to generate barcoded RNA-seq libraries using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The library was prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, #E7760) and amplified using NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 2 ...
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bioRxiv - Zoology 2023Quote: ... ACPR ECL2 and ACPR ECL3) were assembled into pBudCE 4.1 Gα15 using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, Whitby, ON) following the protocol guidelines ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA libraries were generated from 500 ng of total RNA using the NEBnext Ultra II RNA Library Prep kit (New England Biolabs), following the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2023Quote: ... then ligated into linearized plasmid backbones using an NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs Cat. No. E5520S). Recombinant plasmids were transformed into E ...
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bioRxiv - Cancer Biology 2023Quote: Double-barcoded lentiviral vectors were generated from FU-CGW by sequentially inserting matched 10-nucleotide barcodes into the PacI site distal to the HIV FLAP using the Quick Ligation Kit (New England Biolabs) and PCR amplification of the WPRE sequence and barcode with insertion into the ClaI sites proximal to the 3’ LTR by HiFi DNA Assembly (New England Biolabs) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We prepared the sample libraries with NEBNext DNA Library Prep kit for Illumina according to the manufacturer’s protocol (New England Biolabs E7645). We sequenced the samples with the Illumina 4000 HiSeq 150PE ...
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bioRxiv - Microbiology 2023Quote: ... Secreted cypridina Luciferase (cLuc) activity from the internal control plasmid was similarly measured using the cypridina Luciferase kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... RNAseq libraries were constructed by the Transcriptional Regulation and Expression (TREx) Facility at Cornell University using the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: ... DNA removal coupled with cDNA synthesis were conducted using NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s recommended protocol ...
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bioRxiv - Microbiology 2023Quote: ... then used as template to generate cDNA using NEBNext Ultra RNA Library Prep Kit for Illumina (England Biolabs, Ipwich MA). The resulting cDNA assessed with a high-sensitivity Agilent 2100 bioanalyzer ...
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bioRxiv - Genomics 2023Quote: Constructs encoding either a GFP or an RFP protein were PCR amplified and in-vitro transcribed from a T7 promoter with the HiScribe T7 Kit (NEB), using a mixture of ATP ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting 1556 bp fragment was inserted into the pET28c vector digested with EcoRI and NcoI using the NEBuilder HiFi Assembly kit (NEB).
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bioRxiv - Developmental Biology 2023Quote: ... sgRNA synthesis was carried out using NEB T7 High Yield RNA Synthesis kit (New England Biolabs France Genopole, Evry, France), following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... according to the manufacturers’ instructions and converted to cDNA using ProtoScript First Strand cDNA Synthesis Kit (#E6300 New England BioLabs). Quantitative PCR (qPCR ...
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bioRxiv - Plant Biology 2023Quote: ... Each of the 11 genes were cloned into a pENTR-DTOPO vector and finally into a pIX-HALO expression vector using a Gateway cloning kit obtained from NEB and following the manufacturer’s protocol (Supplemental Datasets S9-S19) ...
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bioRxiv - Molecular Biology 2023Quote: ... and size selected by running samples on a 1.8% LMT agarose gel at 120 V for 25 minutes at 4°C and gel extracting the mononucleosome band with a Monarch Gel Extraction Kit (T1020S, New England Biolabs). Purified samples were carried forward for qPCR or library preparation ...
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bioRxiv - Cell Biology 2023Quote: ... double and triple N>G mutations were introduced in the parental constructs by using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) according to the manufacturers protocols and using custom designed primer ...
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bioRxiv - Microbiology 2023Quote: ... and given barcode sequences using the NEBnext Ultra-directional RNA Library Kit for Illumina (New England Biolabs, Ipswitch, MA, USA) with NEBnext Multiplex Oligos for Illumina (Primer Set 1 ...
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bioRxiv - Microbiology 2022Quote: ... Illumina sequencing libraries for each genome were constructed using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) using the manufacturer’s protocol with DNA input concentrations of 5-15 ng/µL ...
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bioRxiv - Microbiology 2023Quote: ... Cells were centrifuged 7,000xg/2min pellet was washed in 1ml of PBS and then resuspended in 400 μl of the resuspension buffer (Monarch Miniprep kit – NEB) containing 50 μg of lysostaphin ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing library was generated using NEB Next® Ultra TM DNA Library Prep Kit for Illumina (NEB, USA, Catalog#: E7370L) following manufacturer’s recommendations and index codes were added to each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... The remaining steps of library preparation were done using and the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). Adapters and PCR primers were purchased from New England BioLabs.
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bioRxiv - Molecular Biology 2023Quote: ... Nucleotide substitution or deletion mutations were designed on the NEBaseChanger online tool and TOPO-cloned using the Q5 site-directed mutagenesis kit (New England Biolabs) according to the standard protocol.
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bioRxiv - Molecular Biology 2023Quote: ... . Site-directed mutagenesis was performed on 10 ng pET28a(+)-HisPI31wt template with the Q5 Site-Directed Mutagenesis Kit (New England BioLabs) according to manufacturer’s instructions ...