Labshake search
Citations for New England Biolabs :
6951 - 7000 of 9547 citations for QuantiChrom Phosphate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... with a 47-bp DNA region with flanking SpeI and XbaI sites by using a Q5 mutagenesis kit (New England Biolabs). MBP-RT-8XHis ORFs were inserted into pCas9SX by PCR amplifying the pBL1-MBP-RT-8XHis ORFs with primers that introduce flanking SpeI and XbaI sites and cloning the PCR product between the corresponding sites of pCas9SX.
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bioRxiv - Microbiology 2022Quote: ... from the original pSAG1-Cas9-GFP-UPRT (Shen et al., 2014)) for one targeting the LFM1 locus using the Q5 site-directed mutagenesis kit (NEB). 1 µg of the cassette and 1 µg of Cas9 plasmid were transfected into the LMF1-HA cell line using the Lonza nucleofection system ...
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bioRxiv - Microbiology 2022Quote: ... Indexed libraries were prepared from the resulting RNA using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). Samples were pooled and sequenced on a NextSeq 500 sequencer (Illumina).
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bioRxiv - Microbiology 2022Quote: ... xanthus cells grown on 1.5% agar supplemented with 1% CTwas extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs (NEB)) ...
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bioRxiv - Microbiology 2022Quote: ... These vectors were generated by mutating the sgRNA site in the pSag1-Cas9-U6-sgUPRT-HXG plasmid [14] using the Q5 Site-Directed Mutagenesis Kit (NEB). The two guide RNAs were designed to target TgEFP1 at either of the predicted EF-hand domains using the online E-CRISP tool (http://www.e-crisp.org/E-CRISP/) ...
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bioRxiv - Microbiology 2022Quote: ... The rRNA-depleted samples were used for stranded library preparation using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). The libraries were sequenced on a HiSeq2500 (Illumina ...
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bioRxiv - Microbiology 2022Quote: In order to generate the MksG mutants (MksGE236A, MksGD279A, MksGY258A, MksGY276A) site-directed mutagenesis (Q5 site-directed mutagenesis Kit, NEB) was performed ...
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bioRxiv - Microbiology 2022Quote: ... we executed mutagenic PCR oligonucleotide primers KT1604 and KT1606 and the Q5® Site-Directed Mutagenesis Kit (New England Biolabs) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... Mutants were sequenced using Illumina Miseq and libraries were prepared using the DNA Ultra II library preparation kit (New England Biolabs). Mutations and depth were mapped using CLC-Bio Workbench v8 78.
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bioRxiv - Microbiology 2022Quote: ... The fragment library was prepared using the NebNext Ultra II DNA Library preparation kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and cDNA libraries containing unique barcodes generated from 100 ng mRNA using the Next UltraII Directional RNA Library Prep kit for Illumina (New England Biolabs). The individual cDNA libraries were assessed for quality and quantity by Qubit ...
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bioRxiv - Immunology 2022Quote: RNA was extracted from IgG1 stimulated B cells 72 hour post-stimulation using TRIzol reagent (Fisher) and cDNA was prepared using the Protoscript II kit (NEB). Germline transcription was analyzed using quantitative PCR with SYBR Green (Roche ...
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bioRxiv - Microbiology 2022Quote: ... To perform the qRT PCR we followed the recommendations of the manufacturer Luna Universal One-Step qRT-PCR Kit (New England BioLabs Inc. ...
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Promoter-adjacent DNA hypermethylation can downmodulate gene expression: TBX15 in the muscle lineagebioRxiv - Molecular Biology 2022Quote: Reporter gene constructs were prepared by overlap extension PCR (Table S5) or by using the Gibson assembly kit (NEBuilder HiFi Assembly, New England Biolabs) as previously described [28] ...
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bioRxiv - Molecular Biology 2022Quote: ... The other molecular cloning trials and plasmid gene replacements were performed by Gibson’s assembly with Gibson Assembly® Cloning Kit (NEB). The procedure used to generate each plasmid construct is described in the corresponding subsection of Material and Methods.
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bioRxiv - Molecular Biology 2022Quote: ... Two reactions of 2-4 µg DNA each were adaptor-ligated and indexed using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) and NEBNext Multiplex Oligos for Illumina (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: ... Second-strand cDNA was synthesized in a reaction mixture containing Second-Strand Synthesis Kit (New England Biolabs; Ipswich, MA, USA). cDNA was purified using the Qiagen purification kit ...
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bioRxiv - Microbiology 2022Quote: ... The linear recombineering product was PCR amplified from the resulting plasmid using Phusion High-Fidelity Polymerase and primers MRS09 + MRS10 and PCR purified using the Monarch PCR & DNA Cleanup Kit (T1030, NEB). M ...
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bioRxiv - Microbiology 2022Quote: ... The first primer pair includes a multiple cloning site and both fragments were assembled using NEBuilder Hifi Assembly Cloning kit (New England Biolabs) together with the promoter and 5’ UTR of the constitutive expressed flavodoxin C4N14_09865 amplified from genomic DNA of F ...
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bioRxiv - Microbiology 2022Quote: A plasmid encoding an eGFP-containing MARV antigenome was generated through assembly of fragments using the NEBuilder HiFi DNA assembly cloning kit (New England Biolabs). The vector backbone ...
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bioRxiv - Systems Biology 2022Quote: Library preparation was performed with NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries of ovule samples were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) using NEBNext® Multiplex Oligos for Illumina (Index Primers Set 1 and Index Primers Set 2 ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized from total RNA and used for library construction with the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: RNA libraries were prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module and the NEBNext Ultra II Directional RNA Library Prep Kit (NEB), according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... these fragments were then cloned into pEarleyGate 301[49] (predigested by XbaI and NcoI) using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB). All clones were validated by sequencing ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was performed from 1µL of template RNA in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) on a QuantStudio 6 Flex thermocycler (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2023Quote: ... were constructed using the NEBNext Ultra II DNA library preparation kit for Illumina according to the manufacturer’s instructions (NEB E7645S). To determine the number of PCR cycles required for amplification ...
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bioRxiv - Molecular Biology 2023Quote: ... Ribosomal-depleted RNA was then used for library preparation using NEBNext Ultra II Directional RNA Library Prep kit (NEW ENGLAND BioLabs) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... is inserted in n-terminal of E2 protein of CHIKV 181/25 structural genes (C-E3-E2-6K-E1) and cloned into pcDNA3.1(+) expression vector using gibson assembly cloning kit (NEB, USA). The resulting plasmid is designated as ChAC-VLP ...
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bioRxiv - Microbiology 2022Quote: ... Mutations to the specificity of guides in pTREX-n-Cas9 were performed using a Q5 mutagenesis kit (NEB, Ipswich, Massachusetts). Guides targeting the ELO genes were inserted using the primers described in Table 1 ...
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bioRxiv - Microbiology 2023Quote: RNA samples were depleted for ribosomal RNA using the NEBNext Bacteria rRNA Depletion Kit (New England Biolabs, Ipswich, Massachusetts, USA). Samples were pooled into a library using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... and libraries for deep sequencing were prepared using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs). Quality of DNA libraries was validated using a 2100 Bioanalyzer Instrument (Agilent) ...
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bioRxiv - Plant Biology 2023Quote: ... and the purified mRNA was used to construct libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) following manufacturer instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... A 5 µL aliquot was taken in which primers and dNTPs were then inactivated using Exo-CIP Rapid PCR Cleanup Kit (NEB). From the resulting mixture ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg of purified DNA of each template including the T7 promoter at the 5’ end was used following the manufacturer instructions (HiScribe T7 High Yield RNA Synthesis kit, NEB). Primers used are listed in Supplementary Table 1 ...
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bioRxiv - Zoology 2023Quote: ... Library concentration and quality were assessed with the Qubit Fluorometer and Bioanalyzer and molarity was estimated via qPCR with the NEBNext Library Quant Kit (New England Biolabs). Libraries were single indexed with NEBNext Multiplex Oligos (New England Biolabs ...
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bioRxiv - Genomics 2022Quote: ... We converted the DNA into an Illumina sequencing library using the NEB Ultra II library preparation kit (NEB, Ipswich, MA) with a Y-adaptor ...
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bioRxiv - Genomics 2022Quote: ... The ploidy of the GM12878 and Detroit 532 were confirmed via NGS as follows: sequencing libraries were generated using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs), and libraries were sequenced on a MiSeq (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... Presence of SARS-CoV-2 RNA was determined by using CDC primers and probes with LunaScript RT Supermix Kit (NEB) run on BioRad (Hercules ...
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bioRxiv - Immunology 2022Quote: ... The cDNA libraries were prepared using NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina 96 reactions (New England Biolabs). Libraries were quantified using the KAPA Library Quantification Kit (Roche ...
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bioRxiv - Microbiology 2022Quote: ... The LepB-CutFC-A and LepB-CutFΔC-ter versions were constructed by using the Q5 mutagenesis Kit (NEB Lab, MA) with cutF(CtoA)-F/ cutF(CtoA)-R and lepB-cutF(dC-ter)-F/ lepB-cutF(dC-ter)-R mutagenic primer pairs carrying the CxxxC to AxxxA and Δ10 amino acids from C-terminal proline-rich segment respectively (Table S2) ...
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bioRxiv - Genetics 2022Quote: Libraries for RNA-seq analysis were prepared using 500 ng of total RNA from three independent replicates using NEBNext Ultra II Directional RNA library prep kit for Illumina (NEB) and PolyA mRNA magnetic isolation module (NEB) ...
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bioRxiv - Genomics 2022Quote: ... and taken forward for library preparation using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). A total of 4 libraries were generated ...
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bioRxiv - Genomics 2022Quote: ... adapters and the Sau3AI overhang adapters (375 pM) in a 40 μL solution using 50 units of Quick Ligase (Quick Ligation Kit; NEB) that was 1X reaction buffer ...
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bioRxiv - Genomics 2022Quote: ... and 100 ng of each PCR product was used to generate the final libraries using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). The libraries were indexed for multiplexing using NEBNext multiplex oligos kit for Illumina (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: Detection of viral genomes from heat-inactivated samples was performed by RT-qPCR using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) with SARS-CoV-2 specific primers targeting the N gene region (5′-TAATCAGACAAGGAACTGATTA-3′ and 5′-CGAAGGTGTGACTTCCATG-3′ ...
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bioRxiv - Microbiology 2022Quote: ... FastDigest restriction enzymes (Thermo-Fisher) were used for plasmid digestion and Gibson assembly was performed using the NEBuilder HiFi DNA assembly kit (NEB). All constructs were verified by DNA sequencing.
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bioRxiv - Microbiology 2024Quote: ... On-bead PCR indexing-amplification was performed using custom-ordered indexing primers (IDT) matching the Illumina Nextera Index Kit sequences and 2x Phusion Master Mix (NEB). PCR reactions were amplified for 9-11 cycles ...
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bioRxiv - Microbiology 2024Quote: To test the binding affinity of the TBT and TBTG mRNA to the 30S ribosomal subunit we used PURExpress ΔRibosome Kit (NEB) supplemented with 5 μM of 30S ribosomal subunit and 10 μM tRNAfMet in the presence of 1.4 μM of radioactively labelled mRNA (prepared as described above by in vitro translation followed by [32P] labelling as described for the northern blot probe labelling) ...
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bioRxiv - Microbiology 2024Quote: ... Genes of interest were PCR amplified to include either their native or heterologous promoter and cloned using Gibson Assembly kit (NEB) into integration vectors pDG1662 (for insertion into the amyE locus) ...