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Citations for New England Biolabs :
6951 - 7000 of 7781 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... was detected by addition of 1 nM Europium-labeled anti-p53 phosphoserine 15 antibody (New England Biolabs/ Cisbio) and 40 nM streptavidin-conjugated APC (Prozyme ...
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bioRxiv - Molecular Biology 2021Quote: ... mRNA was isolated from 1 µg total RNA using the NEB Poly(A) mRNA Magnetic Isolation Module (NEB) and prepared using the Stranded RNA-seq protocol (67) ...
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bioRxiv - Biochemistry 2021Quote: Each cyclization reaction contained the following components: 1 µL 10X T4 DNA ligase reaction buffer (New England BioLabs), 2 µL water ...
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bioRxiv - Biochemistry 2021Quote: ... The following day cells were treated with 1 µM SNAP-cell TMR-Star (New England BioLabs cat. # S9105S) in complete culture media for 45 minutes at 37 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 μ L of Protoscript II Reverse Transcriptase (200U/μ L, Catalog No. M0368, New England BioLabs Inc.), 2 μ L of 0.1M dithiothreitol (DTT) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 μg of DNA library was used as a template for mRNA synthesis by HiScribe T7 kit (NEB). The product was purified by NH4Ac precipitation and dissolved in RNAse-free water to a final concentration of 3 μg/ul.
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bioRxiv - Biochemistry 2021Quote: ... 50 μl of lysed cells were aliquoted to 8 tubes containing 450 μl of digestion mix (1× NEB 1 buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 ug of RNA was reverse-transcribed using the WarmStart® RTx reverse transcriptase (New England BioLabs, M0380L) and random primer mix (New England BioLabs ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl of 100 μM SARS_139 and SARS_140 primers were mixed with 1 μl T4 Polynucleotide kinase (PNK; NEB) in 1x PNK reaction buffer (NEB) ...
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bioRxiv - Neuroscience 2022Quote: ... PCR product was then restriction enzyme digested at 37° C for 1 hour with MseI (New England Biolabs) and then run on a 1.5% Agarose gel ...
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bioRxiv - Molecular Biology 2023Quote: NSD1/2 WT and different cancer mutants (3.4 μM) were mixed with recombinant H3.1 protein (1 μg) (purchased from NEB) or recombinant H3.1 mononucleosomes in methylation buffer (50 mM Tris/HCl ...
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bioRxiv - Cancer Biology 2022Quote: ... Exonuclease I reaction mixture (1X Exo-I buffer, 1 U/µL Exo-I (New England Biolabs, cat# M0293L)) was pipetted into the device followed by an incubation at 37 °C for 45 min ...
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bioRxiv - Molecular Biology 2022Quote: ... The reverse transcriptase reaction was performed using between 100 ng and 1 μg RNA per sample (NEB, E3010). Quantitative PCR (qPCR ...
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bioRxiv - Cancer Biology 2023Quote: Pellets were resuspended in 99.25 µL micrococcal nuclease reaction buffer (1:10 micrococcal nuclease 10 x buffer (NEB), 1:100 10 mg/mL BSA in distilled water ...
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bioRxiv - Biophysics 2023Quote: ... and subsequently mixed with ∼0.2 ng of coilable ∼3.3 kbp dsRNA and 1 mg/ml bovine serum albumin (BSA, New England Biolabs). The ∼3.3 kbp length includes only the stem ...
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bioRxiv - Microbiology 2023Quote: ... 20 pmol of the obtained RNA were then radiolabeled by incubation with 1 U of Polynucleotide Kinase (NEB) and 20 μCi of 32P-γATP for 1 h at 37°C in a 20 μL reaction volume ...
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bioRxiv - Genomics 2023Quote: ... LIDAR-3_RT_antisense was digested by adding 1 μL of USER II enzyme (New England Biolabs, Cat. No M5508S) and incubating at 37°C for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... RNA was then purified again and the eluate was subjected to circularization with T4 RNA Ligase 1 (NEB). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: The PCR reactions were prepared as follows: Mix 1 μl of Phusion DNA Polymerase (New England Biolabs, M0530), 10 μl of 5x Phusion HF Buffer ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was digested for 1 h at 37°C with PNGase F (New England Biolabs, P0704) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of DNA was incubated 20min at 37°C with 15 units of RNAseH1 (New England Biolabs) and first digested for 2h at 37°C with 35 units of AsiSI restriction enzyme (New England Biolabs) ...
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bioRxiv - Bioengineering 2023Quote: ... 1 μg of total DNA was first digested overnight with 20 U of SmaI (New England Biolabs, R0141) at 25 °C ...
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bioRxiv - Cell Biology 2024Quote: ... gibco) supplemented with 10% fetal calf serum (eurobio scientific) and 1% ZellShield cell culture contamination preventive (Minerva-Biolabs). Cells were passaged at 80-90% confluency ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated with primary antibody rabbit anti-c-Fos (1:3000, New England Biolabs cat. No. 2250S) for 24 hours at room temperature ...
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bioRxiv - Genomics 2023Quote: ... and NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors RNA Set 1) (New England BioLabs E7416). DNA libraries were quality checked with DNA 1000 assay (Aligent 5067-1504 ...
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bioRxiv - Synthetic Biology 2023Quote: ... <1 ng of DNA of interest was pipetted to a mix of 1x rCutsmart buffer (New England Biolabs), 100 μM of primer ...
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bioRxiv - Genetics 2023Quote: ... Gibson assembly was performed for 1 hour at 50°C using Gibson Assembly Master Mix (New England Biolabs), 500 ng backbone ...
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bioRxiv - Developmental Biology 2023Quote: ... comparing the intensities of the major dsRNA bands to known amounts of 1 kb DNA ladder marker (NEB) bands using Fiji (Schindelin et al ...
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bioRxiv - Genetics 2023Quote: ... Libraries were indexed with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (New England BioLabs E7600S). Completed libraries underwent quality assessment on the Agilent Fragment Analyzer run by JH SCTC ...
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bioRxiv - Cancer Biology 2024Quote: ... PTEN and PD-1 were subcloned into linearized pLJM1-empty vector using EcoR1 and NHE1 restriction enzymes (NEB) and Takara’s two-step In-Fusion® snap assembly (#638948 ...
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bioRxiv - Neuroscience 2023Quote: ... 10µL ligation mix was prepared (20ng digested pX552 vector, 0.8µL 1:50 diluted oligo mix, 5µL 2X T7 Buffer (BioLabs #B0318S), 0.5µL T7 DNA Ligase (BioLabs #M0318S ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... P5 adapters were ligated to the ends with 1 μl of 2000 U/μl T4 DNA Ligase (NEB) at 16 °C for 20 hr ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... The wash solution was then replaced with 1× T4 polymerase buffer containing 30 U T4 DNA polymerase (NEB) and incubated at 12 °C for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Dot blot samples were prepared from 1 µg gDNA with or without RNaseH1 2500 U treatment (NEB, M0297) O/N 37°C.Samples were denatured in Denaturation Buffer (NaOH 400 mM ...
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bioRxiv - Cell Biology 2023Quote: Equal amounts (25 μg) of whole normal muscle extracts were incubated with 1 x Glycoprotein denaturing buffer (Biolabs) at 100 °C for 10 min to denature glycoproteins ...
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bioRxiv - Biochemistry 2023Quote: ... was assessed with the same protocol but in 1 X ThermoPol Buffer (New England BioLabs Inc. Cat#B9004S).
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bioRxiv - Biochemistry 2023Quote: ... was assessed with the same protocol but in 1 X rCutSmart Buffer (New England BioLabs Inc. Cat#B6004S) and incubated at 65 [ ...
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bioRxiv - Immunology 2023Quote: ... for 1 hour at 37°C and ligation was performed using the T4 RNA ligase I (NEB M0204) for 2 hours at 25°C ...
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bioRxiv - Biophysics 2024Quote: ... dsDNA template was prepared by annealing of WT_sPTC_F and WT_sPTC_R ssDNA oligos (Supplementary Table 1) followed by DNA synthesis using Klenow Fragment of DNA polymerase I (NEB). For annealing ...
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bioRxiv - Molecular Biology 2022Quote: ... For size reference 1 kb and 100 bp DNA ladders (New England Biolabs, catalog no. N3232 and N3231) were combined and loaded onto the gel ...
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bioRxiv - Biophysics 2022Quote: ... 1 μL of 100 mM 18O4-γ-ATP followed by addition of 20 U of T4-PNK (NEB) and incubation for 1 h at 37 °C ...
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bioRxiv - Biophysics 2022Quote: ... 40 μL of pooled oligonucleotides (100 μM) were mixed with 12 μL ddUTPs (1 mM, New England Biolabs), 2.4 μL TdT (20000 U/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1, NEB, E7335L). Conditions for PCR used are as follows ...
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bioRxiv - Developmental Biology 2022Quote: ... and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1, NEB, E7335L). Conditions for PCR used are as follows ...
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bioRxiv - Microbiology 2022Quote: ... The digestion product was then treated with Antarctic phosphatase for 1 hour at 37°C (New England Biolabs). PCR products were purified using the Zymogen Clean and Concentrator PCR-cleanup kit (Zymo Research ...
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bioRxiv - Plant Biology 2022Quote: ... and the ubiquitinated ERECTA_CD were detected by IB analysis with anti-MBP (E8032, 1:10,000, New England Biolabs) as primary antibody ...
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bioRxiv - Bioengineering 2023Quote: ... 0.27 nmol ErCas12a/LbCas12aU protein and 0.45 nmol crRNA were assembled in 1 X Nuclease Reaction Buffer (NEB). The protein and RNA were mixed and incubated for 10 minutes at room temperature and used for transformation of embryogenic C ...
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bioRxiv - Biochemistry 2022Quote: ... The sequence corresponding to residues 1-239 was amplified by PCR using Q5 High-Fidelity DNA Polymerase (NEB), cut with restriction enzymes NdeI (NEB ...
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bioRxiv - Bioengineering 2022Quote: ... the IP buffer was adjusted to 1% SDS and 0.8 mg Proteinase K (New England Biolabs, Ipswich, MA) was added ...
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bioRxiv - Cancer Biology 2023Quote: ... and samples were barcoded using the NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) (NEB), listed in the Supplementary Data 1 ...