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Citations for New England Biolabs :
6901 - 6950 of 7781 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was carried out on 1 μg total RNA after DNase I-digestion (New England Biolabs) using the SuperScript III kit (Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... and libraries prepared using NEBNext Multiplex Oligos for Illumina Index Primer Set 1 (New England Biolabs, E7335S) and NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... followed by a secondary Alexa Fluor 555 conjugated anti-mouse IgG antibody (NEB #4413S; diluted 1:1000). For flow cytometry ...
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bioRxiv - Microbiology 2024Quote: ... The phages were then treated with 100 μg·ml-1 of Proteinase K (New England Biolabs, Whitby, Canada) with SDS to a final concentration of 2% (v·v-1 ...
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bioRxiv - Plant Biology 2024Quote: ... After washing twice with nuclei isolation buffer and one round with 1× NEB Dpn II buffer (NEB), nuclei pellet was resuspended with 250 µl 0.5% SDS and split into five tubes ...
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bioRxiv - Cell Biology 2024Quote: ... 0.8 M guanidine HCl) containing 8 units mL−1 of Proteinase K (P8107S, New England Biolabs Inc.). Gels were digested at room temperature overnight ...
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bioRxiv - Genomics 2024Quote: ... The RCA-MERFISH probes were ligated with 10% v/v (∼1 uM) SplintR ligase (New England Biolabs), 1× SplintR ligase buffer ...
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bioRxiv - Bioengineering 2024Quote: ... ddPCR reactions were incubated at room temp for 1 hour with 1E5 units of SalI (NEB R3138L) to facilitate digestion of linear cAAV insertion products prior to droplet generation ...
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bioRxiv - Biochemistry 2024Quote: RT-PCRs were performed on 1:100 diluted RT samples using hot start Taq DNA polymerase (NEB) supplemented with extreme thermostable ssDNA-binding protein (ET SSB ...
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bioRxiv - Biochemistry 2024Quote: ... TdT (20 U) and α-32P-dATP (3.3 pmol) were prepared in 1 x TdT buffer (NEB) and incubated at 37 °C for 1 h ...
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bioRxiv - Genomics 2024Quote: ... and ligated with methylated adapters for Illumina sequencing at a dilution of 1:10 (New England BioLabs). The adapter ligated DNA was subjected to bisulfite conversion with EpiTect fast bisulfite conversion kit (Qiagen ...
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bioRxiv - Systems Biology 2024Quote: ... each with 40μL of FACS buffer with 1:1000 diluted Murine RNase Inhibitor (New England Biolabs, M0314L). After collection ...
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bioRxiv - Synthetic Biology 2024Quote: ... was mixed with 0.5 µL of ATP (100 mM) and 1 µL of T4 PNK (NEB, UK) with a 33.5 µL normalized DNA solution (approx ...
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bioRxiv - Biochemistry 2024Quote: ... Denatured complexes were run on a 1% agarose gel containing 1X Sybr Safe stain (New England Biolabs).
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bioRxiv - Cancer Biology 2024Quote: ... The guides were amplified by PCR and cloned into pKLO.1 by ligation using the Esp3I (NEB) restriction sites39,40 ...
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bioRxiv - Genetics 2021Quote: ... 1 μg of RNA was reverse transcribed in cDNA using random hexamers and M-MuLV reverse transcriptase (NEB). qPCRs were assembled with Absolute QPCR ROX Mix (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Backbone and annealed guide template were ligated for 1 h at room temperature using T4 DNA Ligase (NEB), 100 ng backbone ...
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bioRxiv - Evolutionary Biology 2021Quote: Hybridization with the reverse transcription primers was performed by adding 1 uL of SR RT Primer (NEB E7333A) to the above reaction and incubating at 75 C for 5 minutes ...
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bioRxiv - Evolutionary Biology 2021Quote: Both HLS-C and HLS-E protein at 1 mg/mL were incubated with human Furin (EC 3.4.21.75, P09958, obtained from NEB P8077) in digestion buffer (10 mM HEPES (pH 7.5) ...
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bioRxiv - Developmental Biology 2020Quote: ... 2014) by incubating 0.25 μl of plasmid with 1 μl of recombinant Cre recombinase (New England Biolabs M0298S) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: One μL of amplicons from colony PCR is mixed with 1 μL of Gel Loading Dye (6x; NEB) and 4 μL of nuclease-free water in each well on the 1.2% agarose gel (Sigma ...
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bioRxiv - Genomics 2020Quote: ... except that all ligations were supplemented with 1:40 RNAse inhibitor (ThermoFisher Ribolock or NEB Murine RNase Inhibitor) to prevent RNA degradation ...
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bioRxiv - Genetics 2021Quote: ... we used NEBNext® Multiplex Small RNA Library Prep Set for Illumina®(Set 1) (catalog#E7300S,NEB) (as described13) ...
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bioRxiv - Biochemistry 2020Quote: ... Cas9 RNPs were formed by incubating 1 pmol of sgRNA with 0.5 pmol of Cas9 protein in 1X NEBuffer™ 3.1 or 2.1 (NEB) at room temperature for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... 8 μL of RNA was added per section to step 2.1 through to 2.11.11 of the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB) using NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 ...
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bioRxiv - Biophysics 2020Quote: ... followed by treatment for 1 h at 37 °C with 2.5 units of RNase-free DNase I (NEB). The sample was once again cleaned with RNeasy MinElute Cleanup Kit before applying on a 1% agarose gel for gel extraction and purification with QIAGEN gel extraction kit and elution with RNase free H2O ...
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bioRxiv - Synthetic Biology 2021Quote: ... we used either a DNA ladder (Low Molecular Weight DNA Ladder or 1 kb Plus DNA Ladder, NEB) or an RNA ladder (RiboRuler Low Range or High Range RNA Ladder ...
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bioRxiv - Synthetic Biology 2021Quote: ... The template was eliminated after the PCR reactions by addition of 1 µL DpnI (10,000 U/mL, NEB) to 25 µL PCR reactions and incubation at 37 °C for at least 1 h ...
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bioRxiv - Physiology 2020Quote: ... and analysed by Western blotting using antibodies for TCF4/TCF7L2 (C48H11) (#2569, 1:500, Cell signalling, NEB, UK), phospho-AKT (#9271 ...
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bioRxiv - Plant Biology 2020Quote: ... PCR enrichment of adaptor ligated DNA was conducted using NEBNext Multiplex Oligos for Illumina (Set 1, NEB#E7335). The PCR reaction was purified using Agencourt AMPure XP Beads ...
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bioRxiv - Biophysics 2020Quote: Each reverse transcription (RT) reaction was performed using 50 ng total RNA in 1 mM dNTPs (NEB N0447S), 10% DMSO (Fisher ...
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bioRxiv - Microbiology 2021Quote: ... 100ng of vector was digested for 1 hour at 37°C with 10 U of BamHI-HF (NEB) to create a single double-stranded cut or 10 U Nb.BtsI (NEB ...
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bioRxiv - Cell Biology 2021Quote: SNAP-GCGR was detected with an anti-SNAP-tag rabbit polyclonal antibody (P9310S, New England Biolabs, 1/500) followed by goat anti-rabbit IgG H&L HRP (ab6271 ...
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bioRxiv - Biochemistry 2022Quote: AP-DNA was prepared by incubating 1 μM uracil-containing oligonucleotide with 0.6 U UDG (New England Biolabs) (55 ...
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bioRxiv - Biochemistry 2022Quote: ... and each RV segment was then transcribed using 1 μg of linearized DNA with HiScribe T7 from NEB at 37°C for 3 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Complexes of TtAgo programmed with ssDNA guides were prepared by combining final concentrations of 1 pmol TtAgo (NEB) and 2 pmol 5’-phosphorylated ssDNA guides and incubating at 70 °C for 20 minutes ...
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bioRxiv - Neuroscience 2021Quote: U251 MG (KO) cells and NSC-34 cells were collected into 1× SDS Blue loading buffer (NEB; #B7703S) and heat-treated at 95°C for 5 min ...
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bioRxiv - Bioengineering 2021Quote: ... fixed cells were incubated in DNAse I buffer (New Englab BioLabs, M0303S, 1:100 dilution in PBS buffer) for 30 min at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... 50 nM of each of the 27f and 534r primer mixes and 0.025U.μl−1 Taq DNA polymerase (NEB). The cycling parameters were 95 °C for 2 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... Around 500ng of RNA were ligated overnight at 16°C using 20units of T4 RNA ligase 1 (NEB) in the presence of 10% PEG 8000 to favour intramolecular ligations ...
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bioRxiv - Molecular Biology 2020Quote: ... Around 500ng of RNA were ligated overnight at 16°C using 20units of T4 RNA ligase 1 (NEB) in the presence of 10% PEG 8000 to favour intramolecular ligations ...
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bioRxiv - Immunology 2021Quote: ... 1 µL of ProtoScript II reverse transcriptase 200 U / µL (cat. n° M0314L NEB, New England Biolabs, USA), 2 µL of random primer mix 60 µM (cat ...
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bioRxiv - Immunology 2021Quote: ... 1 µL of ProtoScript II reverse transcriptase 200 U / µL (cat. n° M0314L NEB, New England Biolabs, USA), 2 µL of random primer mix 60 µM (cat ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The reaction was incubated for 1 hour at 37 °C and stopped by adding 6X loading dye (NEB) with EDTA and run 2% agarose gel ...
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bioRxiv - Synthetic Biology 2020Quote: ... One acceptor and one or more donors were mixed with a ratio of 1:1.1 in distilled H2O with 0.5 ul (7.5 U) of CRE recombinase (NEB # M0298M) and 1 ul Cre buffer (provided with CRE recombinase ...
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bioRxiv - Molecular Biology 2020Quote: ... A ligation mastermix was made consisting of 1 μl T4 DNA Ligase (NEB, cat#M0202S, 400 U/μl), 1 μl of T4 DNA ligase buffer (10x stock ...
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bioRxiv - Microbiology 2021Quote: ... The ~1 kb fragment was then digested with restriction enzymes EcoRI and HindIII (New England Biolabs, Ipswitch MA), purified using Fisher’s GeneJET Gel Extraction and DNA Clean Up Kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg of RNA was used for cDNA synthesis using the ProtoScript® II kit (New England BioLabs). qPCR reactions were carried out with FastStart Essential DNA Green Master (Roche ...
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bioRxiv - Developmental Biology 2020Quote: ... The probes were subjected to a 1:30 dilution of uracil-specific excision reagent (USER) enzyme (NEB, N5505S) for about 24h at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Up to 1 μg of DNA-free RNA was reverse transcribed using ProtoScript II Reverse Transcriptase (NEB, M0368) and anchored oligo dT primers according to the product instructions.