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Citations for New England Biolabs :
6601 - 6650 of 7781 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... 250 ng – 1 μg of RNA samples were digested with Nucleoside Digestion mix (NEB, M069S) for 2 hr at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... PBT was removed and SNAP substrate diluted in PBT (SNAP-Surface649, NEB S9159S; 1:1000) added ...
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bioRxiv - Genomics 2021Quote: ... into 96-well plate containing 2.5μl of methylase reaction buffer (1 × M.CviPI Reaction buffer (NEB), 2 U M.CviPI (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... and 70 μl water and 1mM MnCl2 added together with 1 μl DNaseI (NEB #M0303S) for 10 minutes at room temperature in order to degrade any DNA that precipitated overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... total RNA (1 µg) extracted from adult mouse testes was treated with alkaline phosphatase (NEB), de-phosphorylated ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were recovered in 1 ml NEB® 10-beta/Stable Outgrowth Medium (NEB, B9035) at 37 °C for 1 hour ...
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bioRxiv - Genetics 2022Quote: ... The supernatant (~12 ml) was added to 1 ml HEGX washed chitin magnetic resin (NEB) and left to bind for 1 h at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... the library was treated with a 1:20 dilution of USER enzyme (New England Biolabs) at 37 °C for 30 min ...
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bioRxiv - Developmental Biology 2021Quote: ... at least 1×106 fixed cells were digested using the DpnII restriction enzyme (NEB, R0543M). Chromatin was re-ligated with T4 ligase (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... with ISPCR (IDT; 0.1 μM) and Lambda Exo (New England BioLabs MO262S; 1 units/reaction) for 12 or 14 total cycles.
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bioRxiv - Microbiology 2021Quote: ... we treated the purified RT reaction with 1 μl of Exonuclease I (NEB, Catalog# M0293L) at 37°C for 30 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... (2019): 1) the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760) was used for library preparation ...
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bioRxiv - Genomics 2022Quote: ... Each “PCR#1” reaction contained 25 µL of Q5 High-Fidelity 2X Master Mix (NEB), 2.5 µL of Nuc PCR#1 Fwd Primer (10 µM) ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μg total RNA was depleted of rRNA using the NEBNext rRNA Depletion kit (NEB). To capture nascent RNA ...
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bioRxiv - Microbiology 2020Quote: ... and 1 μL of 200 U M-MuLV reverse transcriptase enzyme (B0253S, New England Biolabs). Negative controls were reactions without the RT enzyme and without sample ...
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bioRxiv - Microbiology 2021Quote: ... Protein purification was done in eight steps: (1) amylose resin high flow (New England Biolabs) chromatography using a step elution from 0 mM to 10 mM maltose in Tris buffer A (20 mM Tris/HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... and treated amplicons were visualized on ~1% agarose gels with 100 bp DNA ladder (NEB) and SYBR Gold (Thermo Fisher Scientific).
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bioRxiv - Biophysics 2021Quote: ... 1 mM DTT at pH 7.9) containing 30 units/ml of T4 DNA polymerase (NEB), 100μg/ml single stranded DNA binding protein (gp32 ...
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bioRxiv - Microbiology 2021Quote: ... 0.7 μL of 20 mg mL-1 bovine serum albumin (New England Biolabs, MA, USA), 0.75 μL of each primer (10 μM) ...
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bioRxiv - Immunology 2020Quote: DNA encoding SARS-CoV-1 VH regions was amplified by PCR (using Q5 polymerase NEB). J segments were modified as required to match the following protein sequence (GTLVTVSS) ...
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bioRxiv - Molecular Biology 2020Quote: ... Reactions were undertaken in 25 ul consisting of 1 x Long amp Taq buffer (NEB), 300 μM dNTP mix ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were mixed with 1× SDS–PAGE sample loading buffer (New England Biolabs # B7703S) and incubated at 98°C for 5 min ...
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bioRxiv - Neuroscience 2021Quote: We next linearized the vector pscAAV-Hsp68-EGFP (Supplemental Figure 1) using PacI (NEB #R0547L) and AscI (NEB #R0558L) ...
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bioRxiv - Developmental Biology 2021Quote: A working lysis buffer solution was prepared by adding 1 µL of the MNase (NEB) [1:50 dilution] per 5 µL lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... 30 mM acetyl phosphate or 1 µl Calf Intestinal Alkaline Phosphatase (CIP) (NEB, catalog#: M0290V) was added to the solution ...
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bioRxiv - Microbiology 2021Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1) (NEB #E7600) (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg of DNase treated RNA was reverse transcribed with Protoscript II (New England Biolabs) and a no reverse transcriptase (no RT ...
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bioRxiv - Genomics 2021Quote: ... at specific sequence motifs (GCTCTTCN^) in the presence of 1 μl 10X buffer 3.1 (NEB) for 2 h at 50 °C and ultra-pure water to a volume of 10 μl ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA was synthesized from 1 μg of RNA using M-MuLV Reverse Transcriptase (NEB) and random hexamer primer (Promega) ...
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bioRxiv - Neuroscience 2023Quote: ... The primary antibodies used were anti-PDGF-Ra (rabbit, New England Biolabs, 1:400 dilution), anti-APC (clone CC1 ...
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bioRxiv - Cancer Biology 2023Quote: ... agarose plugs containing DNA were washed three times with 1× NEBuffer 3.1 (New England BioLabs) with 5-minute incubations ...
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bioRxiv - Cell Biology 2023Quote: ... and M-MuLV reverse transcriptase enzyme (200,000 U mL−1, New England Biolabs, Ispwich, USA) was added per sample ...
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bioRxiv - Molecular Biology 2023Quote: ... using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer, NEB, 1 µL of 100 µM gapmer ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg of purified DNA was treated with 10 units Exonuclease V (New England Biolabs) in a 50 μl reaction at 37°C for 30 min to remove ssDNA ...
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bioRxiv - Biochemistry 2023Quote: ... and ScFv16 in the presence of 25 mU ml-1 apyrase (NEB, Cat. no. M0398S) and 1 µM of individual ligand ...
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bioRxiv - Biochemistry 2023Quote: ... 1.35 μL of 1 mM dNTPs and 0.8 μL Klenow exo-polymerase (New England Biolabs) were added to the annealed product ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.125 μl of Shrimp Alkaline Phosphatase (1 U/ml; New England Biolabs, cat. no. M0371S), 0.0125 μl Exonuclease I (20 U/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA substrate was incubated with 1 unit of purified Uracil-DNA glycosylase (New England BioLabs) instead of cell extracts in the same reaction buffer ...
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bioRxiv - Biophysics 2024Quote: ... N-glycosylation was removed by a 1-hour incubation with PNGase F (500 U, NEB) and following digest with sequence-grade trypsin (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were then incubated with RNase H (NEB, 1: 50 in 1X RNase H buffer) at 37°C for 4 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... The reaction system was as follows: 20 μL volume containing 1× reaction buffer (NEB, B9012S), 5 U/μL T7 RNA polymerase (NEB ...
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bioRxiv - Genomics 2024Quote: ... 1 μg of the step two plasmid library was linearized with SpeI-HF (NEB #R3133) at 37°C for 16 hours ...
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bioRxiv - Genetics 2024Quote: ... A mixture of 20 ng/μL transgene and 70 ng/μL 1 kb ladder (NEB) filler DNA was microinjected into CZ26711 young adult hermaphrodites ...
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bioRxiv - Immunology 2024Quote: ... RNA samples (1 µg each) were mixed with 2x RNA Loading Dye (New England Biolabs), denatured at 72 °C for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... 0.4 µg μl-1 bovine serum albumin (New England Biolabs® Inc., Ipswich, MA, USA) and 5 µl DNA template ...
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bioRxiv - Developmental Biology 2023Quote: ... After samples were PCR-amplified using 1×NEBNext High-Fidelity PCR Master Mix (NEB, M0541S). Subsequent libraries were purified with the MinElute PCR Purification Kit (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... at 37 °C for 1 hour prior to the addition of T4 DNA ligase (NEB). The ligation reaction was incubated at room temperature for 1 hour then transformed into DH5ɑ competent cells.
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bioRxiv - Molecular Biology 2023Quote: ... The reaction was stopped by adding 1 µL of Thermolabile Proteinase K (NEB, Ipswich, MA) and incubating for 30 min at 37°C then an additional 10 min at 60°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The reaction was stopped by adding 1 µL of Thermolabile Proteinase K (NEB, Ipswich, MA) and incubating for 30 min at 37°C then an additional 10 min at 60°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µL of T4 DNA ligase high-concentration (2,000,000, units/mL, NEB, cat. no. M0202T) was added ...