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Citations for New England Biolabs :
6501 - 6550 of 7781 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... the chromatin was collected and resuspended in 200 μL 1x NEBuffer 1 (NEB, B7001S) and 200 U NEB Exonuclease III (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... chromatin fraction was solubilized in Buffer A with 1:100 micrococcal nuclease enzyme (NEB) and incubated for 15 min at 37°C shaker.
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bioRxiv - Molecular Biology 2021Quote: ... This was repeated with a 1:200 pA-Dam solution (∼60 NEB Dam units), followed by 2 wash steps ...
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bioRxiv - Molecular Biology 2021Quote: ... Component A contained 1 μL of Bst 3.0 DNA polymerase (Cat. No. M0374L, NEB), 2.5 μL of 10 × isothermal amplification buffer ...
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bioRxiv - Neuroscience 2020Quote: ... 1:1000 concentrations of SNAP-Surface 488 (NEB #S9124, New England Biolabs, Ipswich, MA), TMR Halo (G8252 ...
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bioRxiv - Molecular Biology 2020Quote: ... digested overnight at 50°C with 1 μl 20 U/μl SfiI (NEB R0123S) in 200 μl 1x CutSmart buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... digested overnight at 50°C with 1 μl 20 U/μl SfiI (NEB R0123S) then rinsed with 1xTE.
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bioRxiv - Genomics 2022Quote: ... DNA samples were loaded together with Quick-Load 1 kb Extend DNA Ladder (NEB) to aid size determination ...
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bioRxiv - Genomics 2020Quote: ... PCR products were treated with 1 μl of exonuclease (New England Biolabs, 20 units), and the mixture was then incubated at 37°C for 15 min ...
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bioRxiv - Microbiology 2021Quote: ... Denatured RNA was circularised by incubation with 10U T4 RNA Ligase 1 (NEB, USA) in 20 μL of the reaction mixture containing 1x T4 RNA Ligase Buffer (NEB ...
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bioRxiv - Bioengineering 2020Quote: ... PCR 1 conditions: 0.02 U/μL Q5 High-Fidelity DNA Polymerase (New England Biolabs), 1X Q5 reaction buffer ...
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bioRxiv - Neuroscience 2021Quote: Libraries were prepared from 1 ug RNA using a library prep kit (NEB #7770), rRNA depletion kit (NEB #E6310) ...
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bioRxiv - Genomics 2021Quote: ... The template was destroyed by adding 1 μL 20,000 U/mL DpnI (NEB, R0176S) to the mix and incubating at 37 °C for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... Annealed primers were then diluted to 1 µl in 200 µl and ligated (NEB) to the digestedand dephosphorylated Cas9 vector.
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bioRxiv - Microbiology 2021Quote: ... RNA was then removed by incubation with 1 µl of RNase H (NEB, USA) for 20 minutes at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... then directly resuspended in 1× SDS (sodium dodecyl sulphate) sample buffer (New England BioLabs) per 1 ml of OD600 of 1.0 cells in 200 μl sample buffer ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μL PCR products were incubated with 1 μL DpnI enzyme (New England Biolabs) to clean up templates at 37 °C for 2.5 hours and then purified by gel-extraction following the manufacturer instructions (Cat# D2500-02 ...
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bioRxiv - Genomics 2022Quote: 500 ng to 1 μg gDNA were cleaved by SacI-HF (New England Biolabs) for 15 min at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was generated from RNA (Omicron-BA.1; EPI_ISL_7062525) by LunaScript RT SuperMix (NEB). PCR reactions using Q5® High-Fidelity DNA Polymerase were performed with the primers and templates described in Supplementary Information Table 3 ...
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bioRxiv - Immunology 2021Quote: ... 1 µL of 10 mM dNTP (cat. n° N0447L, NEB, New England Biolabs, USA), 0.2 µL of RNase Inhibitor 40 U / µL (cat ...
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bioRxiv - Immunology 2021Quote: ... 1 µL of 10 mM dNTP (cat. n° N0447L, NEB, New England Biolabs, USA), 0.2 µL of RNase Inhibitor 40 U / µL (cat ...
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bioRxiv - Cancer Biology 2020Quote: Specific antibodies against Slug (C19G7, Cell Signaling Technology, NEB, Frankfurt, Germany, #9585, 1:400), pan-cytokeratine (polyclonal ...
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bioRxiv - Genomics 2022Quote: ... and incubated overnight at 37°C with MSPI (20U μl-1 NEB cat: R0106L) and 10x NEBuffer2 (NEB cat ...
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bioRxiv - Microbiology 2022Quote: Fragmented gDNA (1 μg) was end-repaired using an End-repair module (NEB#E6050), and dA-tailed using Klenow (NEB#6053) ...
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bioRxiv - Synthetic Biology 2020Quote: ... we cooled the samples to 37C and added 20U of Exonuclease 1 (NEB, M0293S) to the reaction to remove excess primers ...
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bioRxiv - Developmental Biology 2020Quote: ... Rehybridized product was digested with 1 μL T7 endonuclease I enzyme (New England Biolabs) at 37°C for 1 hour and separated on a 1.5% agarose gel ...
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bioRxiv - Molecular Biology 2021Quote: ... The fragmented RNA was then purified and circularized with T4 RNA ligase 1 (NEB). Tandem repeats were generated during first-strand synthesis at 42 °C for 30 min ...
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bioRxiv - Genetics 2020Quote: ... and 0.3 μL quick ligase in 1 x quick ligase buffer (New England Biolabs) with a total volume of 6 μL ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... 1 μL deoxynucleotide Solution Mix and 0.5 μL Taq DNA Polymerase (New England Biolabs). The reactions were incubated in an Eppendorf Mastercycler Gradient Thermal Cycler for 7m at 95°C ...
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bioRxiv - Biochemistry 2021Quote: ... one of which was incubated with 1 µl nicking enzyme (10 units Nt.BspQI (NEB) or 5 units Nb.Bpu101 (ThermoFisher) ...
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bioRxiv - Biochemistry 2021Quote: ... reaction products were resuspended in 50 µl relevant manufacturer’s enzyme buffer (1 × Cutsmart (NEB) for nicking with Nt.BspQI or 1 × Buffer R (ThermoFisher ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.02% NP-40) including 1 μL of PstI (NEB, at 100,000 U/mL), 2 mM ATP (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) (NEB E7335) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2020Quote: ... The chambers of the microplate were pre-treated with 1 mg/ml BSA (NEB) for 30 min before aspirating the BSA ...
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bioRxiv - Immunology 2020Quote: ... enzymatic digestion was performed 30 min with 1 unit T7 endonuclease (New England Biolabs) at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... Digestion was performed for 1 h using Cut Smart Buffer (NEB, Cat no. B7204S). Next ...
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bioRxiv - Biophysics 2020Quote: ... The flow cell was incubated for 10 minutes with 1 mg/ml streptavidin (NEB) and then 10 minutes with Blockaid (ThermoFisher) ...
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bioRxiv - Bioengineering 2022Quote: ... Each 10 µL experiment contained: 1 µL 10x isothermal buffer (New England Biolabs (NEB)) ...
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bioRxiv - Bioengineering 2022Quote: ... Each 10 µL experiment contained: 1 µL 10x isothermal buffer (New England Biolabs (NEB)) ...
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bioRxiv - Biophysics 2022Quote: ... fluorescently labeled dCas9 was incubated with gRNA in 1× NEBuffer 3.1 (New England Biolabs). 1 nM dCas9 was incubated with 10 nM gRNA for 30 min at 37 °C on an orbital shaker ...
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bioRxiv - Bioengineering 2022Quote: ... we used a 1 kb Plus DNA Ladder for Safe Stains (NEB; catalog # N0559S), Gel Loading Dye ...
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bioRxiv - Microbiology 2022Quote: ... two complementary oligonucleotides (1 μM each) were hybridized in T4 DNA-ligase-buffer (NEB) containing T4 polynucleotide kinase (PNK ...
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bioRxiv - Microbiology 2022Quote: ... To 200 μL of supernatant 1 μL DNase (New England Biolabs, catalog number: M0303S) 1 μL RNaseA (Biomed ...
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bioRxiv - Synthetic Biology 2022Quote: ... and assembled using NEBuilder HiFi DNA Assembly Master Mix (NEB, 1 h 50° C) into backbones previously digested with corresponding restriction enzymes (NEB ...
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bioRxiv - Evolutionary Biology 2022Quote: ... (1) DNA fragments were repaired with the End-Repair enzyme mix (New England Biolabs). (2 ...
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bioRxiv - Biophysics 2022Quote: ... diluted to ∼1 nM in 1x T4 DNA ligase reaction buffer (New England Biolabs), and treated with T4 DNA ligase to circularize the DNA ...
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Spatial visualization of A-to-I Editing in cells using Endonuclease V Immunostaining Assay (EndoVIA)bioRxiv - Molecular Biology 2024Quote: ... Cells were then incubated for 1 hour with anti-MBP antibody (New England BioLabs) solution diluted in calcium containing blocking buffer ...
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bioRxiv - Microbiology 2024Quote: ... Cells were harvested and resuspended with 1:10 volumes of CutSmart Buffer (NEB, # B7204) and 5 µl of 100 mg/ml RNase-A solution (pre-heated at 100°C for 20 minutes) ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1% BSA) with or without Short Cut RNase III (New England Biolabs; 1:150) for 90 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with a 1:150 dilution of RNase III (New England Biolabs) in low-salt buffer (50 mM Tris-HCl pH 7.6 ...