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Citations for New England Biolabs :
6551 - 6600 of 7781 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... 1 μg of the step one plasmid library was linearized with XhoI (NEB #R0146) at 37°C for 16 hours ...
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bioRxiv - Immunology 2024Quote: ... 1 µl per 50 µl pool volume of Thermolabile Exonuclease I (New England Biolabs) was then added and incubated for 4 minutes at 37 °C ...
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bioRxiv - Genomics 2024Quote: ... The digested nuclei were washed and resuspended by 1 x T4 ligase buffer (NEB). Nuclei were then ligated for 4 h at room temperature by adding 1% Triton X-100 ...
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bioRxiv - Biophysics 2023Quote: 12mer arrays (40 ng) were digested with 1 U of MNase (New England Biolabs) in a 10 μL reaction for varying digestion times (2.5 to 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg RNA was reverse transcribed to cDNA using LunaScript RT SuperMix Kit (NEB). qPCR was performed in 20 μl reactions containing diluted cDNA ...
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bioRxiv - Molecular Biology 2023Quote: ... Rabbit polyclonal anti-SNAP-tag (Cat no. P9310S, New England Biolabs, 1:1000 dilution). Secondary antibodies used were ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were then degraded by addition of 1 μL of Proteinase K (NEB #P8107) and incubation at 56°C for 30 min ...
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bioRxiv - Plant Biology 2023Quote: ... and the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1; New England Biolabs) were used as specified by the manufactureŕs instructions ...
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bioRxiv - Microbiology 2023Quote: ... For XRN1 treatment refolded RNA (1 μg) was incubated with 1U XRN1 (NEB, USA) and 10U RppH (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... this was followed by incubation with 1 µl PNGase F (NEB, Ipswich, MA, USA), 2 µl 10% NP-40 (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... Library indexing was done with NEBNex multiplex oligos for Illumina Set 1 (E7335S, NEB) and Set 2 (E7500S ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μL RNAs were mixed thoroughly with 1 μL T4 PNK (10 U, NEB), 1 μL purified TS2126 and 1 μL PAP1 enzyme (NEB ...
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bioRxiv - Cell Biology 2022Quote: 1 μg of total RNA was reverse transcribed with Protoscript II reverse transcriptase (NEB), both according to the manufacturer’s instructions using random hexamer primers ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... The membrane was incubated with HRP-conjugated (1/2,500 New England Biolabs, Ipswich, MA) or IRDye® (1/10,000 LI-COR Biosciences ...
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bioRxiv - Biophysics 2023Quote: ... The resulting reaction product was mixed with 1 μl Exonuclease V (New England Biolabs) and 3 μl of 10 mM ATP in NEB Buffer 4 ...
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bioRxiv - Molecular Biology 2023Quote: ... were added in a total of 50 µl 1× CutSmart buffer (New England Biolabs). The reaction was run for 15 min at 37 °C on a thermocycler ...
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bioRxiv - Genomics 2023Quote: ... cDNA (1 µg) was prepared from total RNA using LunaScript cDNA synthesis mix (NEB), diluted 5 times before using it for qRT-PCR ...
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bioRxiv - Neuroscience 2023Quote: ... The 385 bp PCR product was digested with 1 unit of Hpy188III (NEB #R0622) for 2 hrs at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Streptavidin conjugated DNA was ligated to the appropriate concentration adapter (1:10) (NEB, 7335L). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... and resuspended in 1X DNase I reaction buffer with 1 µL DNase I (NEB) for 15 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) (E7335; NEB) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... in a reaction mix containing 1× T4 RNA Ligase Buffer (New England Biolabs, M0437M), 1U SUPERase•In RNase inhibitor (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... and NEBNext® Multiplex Oligos for Illumina® Index Primers Set 1 (NEB# E6440). The generated DNA libraries were purified using Agencourt® AMPure® XP (Beckman Coulter) ...
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bioRxiv - Genomics 2023Quote: ... a dual-enSERT-2.1 plasmid (1 μg) was digested overnight with PauI (NEB, R0199) at 50°C in rCutSmart Buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The PacI digest was treated with 1 units T4 DNA polymerase (New England Biolabs) and 2 mM dCTP ...
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bioRxiv - Bioengineering 2024Quote: ... 1 µL of T4 ligase and 0.5 µL of T4 polymerase (both from NEB) were added to 20 µL of PBS ...
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bioRxiv - Neuroscience 2024Quote: ... RT-qPCR was performed using the LUNA 1-step RT-qPCR kit (E3005L, NEB) on an AriaMx Real-time PCR system (G8830A ...
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bioRxiv - Microbiology 2024Quote: ... and 0.5 mM dNTPs) and 1 µL of ProtoScript® II Reverse Transcriptase (NEB) was added ...
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bioRxiv - Developmental Biology 2024Quote: ... The seminiferous tubules were washed twice with 1 U DNAse (NE Biolabs, Ipswich, MA) in PBS ...
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bioRxiv - Immunology 2024Quote: ... partial Illumina adaptor sequences were ligated with T4 RNA Ligase 1 (New England Biolabs), followed by a second reverse transcription ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 1 x 107 GCps were incubated overnight with 1500U of HindIII (NEB, R0104T) at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM ATP with 60 U of High Concentration T4 RNA ligase (NEB, M0437M) at 16°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... with or without the addition of 1 μL (1U) recombinant XRN1 (New England Biolabs) and was then incubated for 60 minutes at 37C as previously described (13) ...
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bioRxiv - Cancer Biology 2024Quote: ... Both miRNA-encoding plasmids and MG1 shuttle plasmids were digested using MLU-1 (NEB) and pre-miR-30-based cassette inserts were ligated individually into the MG1 shuttle vector ...
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bioRxiv - Biochemistry 2024Quote: ... Reactions were stopped by adding 2µl of stop buffer (10mg.ml-1 proteinase K (NEB), 1% SDS ...
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bioRxiv - Plant Biology 2024Quote: ... and Unique Dual Index UMI Adaptors RNA Set 1 (E7416S, NEB, Ipswich, MA, USA) were used for library preparation following the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... the following reagents were added to each reaction: 1 mM dNTP (NEB, Cat. #N0447), 20 U RNase Inhibitor (NEB ...
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bioRxiv - Genomics 2024Quote: ... Undesired background modifications were blocked by adding 1 µL ENDOIV (NEB, 10 U/µL) and 500 µM 8-HQ to 5 µg of gDNA ...
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bioRxiv - Genomics 2024Quote: ... 0.5% Triton X-100) containing 1% of 8 U/mL Proteinase K (NEB #P8107S) for 12-16 hours at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... or fluorescently labelled secondary antibodies diluted 1:15000 (Cell Signaling Technology, NEB, Hitchin, UK), for 1 hour at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... 25 μL of PCR reaction was mixed with 1 μL of DpnI (NEB, cat#R0176) at 37°C for 4 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Chromatin was digested in situ using five units of restriction enzyme CviKI-1 (NEB, R0710S) every 100.000 nuclei and RELACS custom barcodes (4 bp UMI + 8 bp RELACS barcode ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then stained with 1 µM of SNAP-Surface-AlexaFluor 647 (BG-A647, NEB) in 4% BSA + 0.1% Tween-20 for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... the dUTP-containing second strand was digested by adding 1 µL of USER enzyme (NEB) and incubating at 37 °C for 15 minutes ...
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bioRxiv - Genetics 2021Quote: ... 1 μl of 10 mM dNTPs and 0.5 μl of OneTaq Polymerase (New England BioLabs). To generate (partially ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 1 μL cDNA was used in a 30-cycle PCR using Q5 DNA Polymerase (NEB) with primer 8 & 9 or 10 & 11 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 1 μL cDNA was used in a 30-cycle PCR using Taq DNA Polymerase (NEB) with primer 4 & 6 or 5 & 6 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Purified products were ligated with 1 µL T4 DNA ligase (protocol as per NEB®) for 10 minutes at room temperature (benchtop ...
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bioRxiv - Bioengineering 2021Quote: ... for 16 cycles annealing at 67 °C was mixed with 1 μL of ExoI (NEB) digested mixture as the template ...
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bioRxiv - Plant Biology 2021Quote: ... total or virion DNA was treated with 1 unit of Mung Bean nuclease (NEB, Massachusetts). The digestion products were analyzed for SEGS-2 episomes as described above.