Labshake search
Citations for New England Biolabs :
6351 - 6400 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... The cDNA for catalytically inactive E447D mutant was generated through mutation of StcEΔ35 using the Q5 Site-Directed Mutagenesis Kit (Cat # E0552S, New England Biolabs) with primers 5’-TCAGTCATGACGTTGGTCATAATTATG-3’ and 5’-ACTCATTCCCCAATGTGG-3’ ...
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bioRxiv - Microbiology 2024Quote: ... DNA fragmentation was performed using the NEBNext® Ultra™ II FS DNA Library Prep Kit (New England Biolabs), an enzymatic fragmentation assay with an average fragment size of 380 bp ...
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bioRxiv - Microbiology 2024Quote: ... Samples were then treated with DNase I and cleaned with the Monarch RNA Cleanup Kit (NEB, Ipswich, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... EM-seq was performed with 100ng of purified genomic DNA using NEBNext® Enzymatic Methyl-seq Kit (NEB, E7120) and following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Digested oligos and vector were gel-purified with Qiagen gel purification kit and ligated by T4 DNA ligase (NEB). Ligate was transformed into Stbl3 competent bacteria (Thermo Fisher Scientific) ...
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Fluoroquinolone-Specific Resistance Trajectories in E. coli and their Dependence on the SOS-ResponsebioRxiv - Microbiology 2024Quote: ... Genomic DNA libraries were created according to the manufacturer’s protocol using NEBNext Ultra II FS DNA Library Prep kit for Illumina (New England BioLabs) in combination with NEBNext multiplex oligos for Illumina (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Genetics 2024Quote: ... ChIP-seq libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) without size selection ...
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bioRxiv - Molecular Biology 2024Quote: ... libraries were prepared for all samples using the NEB Next Ultra II Directional RNA Library Prep Kit (NEB, E7760S) according to manufacturer protocol ...
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bioRxiv - Immunology 2024Quote: Site-directed mutants in this study were generated using a Q5 site directed mutagenesis kit according to the manufacturer’s instructions (NEB). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: 50 ng of DNA template was transcribed using a HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) according to the manufacturer’s protocol but at one-quarter volume (5 µl) ...
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bioRxiv - Microbiology 2024Quote: ... part of the ONT Kit) to the RNA sample using a mix of 3μl NEBNext Quick Ligation Reaction Buffer (New England BioLabs), 0.5μl RNA CS (ONT Kit) ...
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bioRxiv - Microbiology 2024Quote: ... RIG-I and ISG15 were determined using Luna Universal One-Step RT-qPCR Kit according to the manufacturer’s instructions (New England Biolabs). The primer sequences are available upon request ...
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bioRxiv - Neuroscience 2024Quote: The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... The purified DNA samples were then used for library preparation using NEBNext UltraII DNA Library prep kit (NEB, E7370L) and were sequenced at the iGE3 platform of the University of Geneva.
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bioRxiv - Molecular Biology 2024Quote: ... Library preparation was carried out on the purified DNA using NEBNext Ultra II DNA Library Prep Kit (NEB #E7645) and sequenced at 100 million reads per sample on a NovaSeq6000 using 75-nucleotide read length paired-end sequencing.
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bioRxiv - Molecular Biology 2024Quote: ... Library preparation was done using NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (NEB #E7645, #E7103). DNA was sequenced on Illumina HiSeq 2500 platform.
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bioRxiv - Molecular Biology 2024Quote: ChIP-seq libraries were built using an NEBNext® Ultra™ II DNA Library Prep Kit (NEB #E7645S/L) with immunoprecipitated or input DNA ...
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bioRxiv - Developmental Biology 2024Quote: ... The DNA-Seq libraries were prepared using the NEBNext DNA library preparation kit according to the manufacturer’s instructions (E6040, New England Biolabs) and the libraries were sequenced on an Illumina HiSeq 4000 instrument ...
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bioRxiv - Pathology 2024Quote: ... Library preparation was completed on ribo-depleted RNA using the NEBNext Ultra Directional RNA Library prep kit (NEB, #E7420L) using 8 cycles of PCR amplification and indexed using single (i7 ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were purified with DNA Spin and Concentrate kit (Zymo Research, Irvine, CA, D4013 or NEB Monarch, T1030) following manufacturer instructions or gel-purified from kit (Zymo Research) ...
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bioRxiv - Genomics 2024Quote: ... RNA sequencing libraries were prepared using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB) according to the recommended protocol using 1 ng RNA as input material ...
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bioRxiv - Genomics 2024Quote: ... RNA-seq libraries were constructed using the NEBNext® Ultra™ RNA Library Prep Kit (NEB, Ipswich, MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... IVT was performed on the purified PCR product using a HiScribe T7 High yield RNA Synthesis Kit (NEB, E2040S) and purified using a Monarch RNA Cleanup Kit (NEB ...
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bioRxiv - Microbiology 2024Quote: ... Mutations M1 (CAGGTGcAac) and M2 (CAGGTGTACA) in pX_Luc were introduced using Q5 site-directed mutagenesis kit (New England Biolabs). Plasmids expressing DYRK1A or DYRK1A_KR under the control of the CMV promoter were previously described [29].
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bioRxiv - Developmental Biology 2024Quote: ... Deletions of these constructs were constructed using the Q5 site-directed mutagenesis kit according to the manufacturer protocol (NEB). For construction of pMT-ed:Flag:vhh4 ...
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bioRxiv - Molecular Biology 2024Quote: ... All qPCRs were performed using the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA) and a Step One Plus qPCR machine (Life Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from bulk liver tissue using the Monarch total RNA miniprep kit (New England Biolabs, Ipswich, MA) after homogenization with steel beads using a TissueLyser LT bead mill (Qiagen ...
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bioRxiv - Genomics 2024Quote: ... Small RNA libraries were generated using the NEBnext small RNA library preparation kit according to instructions (New England BioLabs). small RNA high-throughput sequencing libraries were sequenced for 101 cycles using NovaSeq X Plus ...
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bioRxiv - Synthetic Biology 2024Quote: ... All connections between genes and linearized vectors were achieved by Gibson assembly (Gibson assembly kit, New England BioLabs, UK). The shell-(csoS2-NM) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Lettuce variant sequences were cloned into retron Eco2 plasmid using Q5® Site-Directed Mutagenesis Kit (New England Biolabs). Primer sequences and annealing temperatures were generated using the NEBaseChanger™ tool.
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bioRxiv - Synthetic Biology 2024Quote: ... RNA strands were transcribed in-house and column-purified using the Monarch® RNA Cleanup Kit (New England Biolabs). The concentration of oligonucleotides was quantified by UV-vis spectrophotometry using a Thermo Fisher Scientific NanoDrop 2000 Spectrophotometer ...
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bioRxiv - Synthetic Biology 2024Quote: ... and the amplified plasmid DNA was extracted using the Monarch® DNA Gel Extraction Kit (New England Biolabs, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... genomic DNA was extracted using the Monarch HMW DNA Extraction Kit for Tissues (New England Biolabs, Ipswitch, MA, USA). Long-read sequencing was performed on the GridION platform (Oxford Nanopore Technologies ...
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bioRxiv - Microbiology 2024Quote: ... illumina sequencing libraries were prepared with a NEBNext®Ultra™DNA Library preparation kit (New England Biolabs, USA) of 500 mg DNA samples from each sample ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng of total RNA was utilized to construct the sequencing libraries using NEBNext Ultra RNA Library Prep Kit for Illumina as per the manufacturer’s protocol (NEB) and paired-end sequencing was performed with NovaSeq 6000 platform (Illumina).
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng of DNA template was transcribed using a HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) according to the manufacturer’s protocol for 11 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... To ensure this we performed the Oligonucleotide Clean-up protocol of the Monarch PCR & DNA Clean-up kit (NEB) on 10 PCR reaction tubes ...
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bioRxiv - Microbiology 2024Quote: ... cloned into the pre-assembled vector using the NEBridge® Golden Gate Assembly Kit (BsaI-HF® v2; NEB). A synonymous point mutation (A17973G ...
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bioRxiv - Microbiology 2024Quote: ... NGS libraries were prepared by NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB, MA, USA) following manufacturer instructions and sequencing was conducted by NovaSeq 6000 (PE150 ...
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bioRxiv - Synthetic Biology 2024Quote: All plasmid E.coli plasmid DNA minipreps were performed using the NEB Monarch Plasmid Miniprep Kit and Protocol (NEB #T1010). Prior to starting the miniprep protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... All reactions were then digested with 1 µl ExoI and purified using the Monarch DNA Gel Extraction Kit (NEB), eluting in 10 µl of elution buffer (10 mM Tris•Cl pH 7.4) ...
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bioRxiv - Microbiology 2020Quote: ... The cDNA libraries were then constructed from 50 ng of depleted RNA using the NEBNext Ultra II Directional RNA library prep kit following manufacturer instructions (NEB). Libraries were size-selected to 200-700 bp using SPRIselect beads and quality was assessed on the Agilent Bioanalyzer using High Sensitivity DNA chips ...
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bioRxiv - Microbiology 2020Quote: ... DNA libraries were prepared using the NEBNext® Ultra™ DNA Library Prep Kit (New England BioLabs Inc., Ipswich, MA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The resulting fragment was inserted into the HindIII-BamHI sites of pJB866 by an NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). The nucleotide sequence of the insert was confirmed by sequencing ...
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bioRxiv - Cancer Biology 2021Quote: ... mRNA Magnetic Isolation Module and libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs). Library quality was checked on an Agilent TapeStation 4150 and quantified by Qubit 2000 fluorometer (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... mRNA Magnetic Isolation Module and libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs). Library quality was checked on an Agilent TapeStation 4150 and quantified by Qubit 2000 fluorometer (Invitrogen) ...
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bioRxiv - Biophysics 2021Quote: ... Indel frequencies at the SaCas9 target site were assessed via a T7E1 assay with the EnGen Mutation Detection Kit (NEB), using the manufacturer’s recommendations ...
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bioRxiv - Biophysics 2021Quote: ... The AcrIIA4 expression vector was modified to express AcrIIA11 with a C-terminal NLS and HA tags using the HiFi Assembly Kit (NEB).
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bioRxiv - Neuroscience 2021Quote: ... were ligated to end-repaired and A-tailed chromatin using components from NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) (25).
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bioRxiv - Cancer Biology 2021Quote: ... RNA was subsequently sent for either library preparation and RNA-sequencing to Genewiz or cDNA preparation via a two-step process beginning with Lunascript RT Supermix Kit (#E3010, New England Biolabs). qRT-PCR was performed on cDNA from samples for genes of interest using the Luna Universal qPCR Master Mix (#M3003 ...