Labshake search
Citations for New England Biolabs :
6301 - 6350 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... pcDNA3.1-ATP6V1H(Δ176-191)-FLAG and pcDNA3.1-ATP6V1H(Δ176-193)-FLAG were generated using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). pENTR-ATP6V1H-FLAG was produced using the pENTR/D-TOPO Cloning Kit (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by strand-specific cDNA NGS library preparation (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, NEB). The size of the resulting library was controlled by use of a D1000 ScreenTape (Agilent 2200 TapeStation ...
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bioRxiv - Genomics 2023Quote: 500 nanograms of total RNA were used for NEBNext® Ultra II Directional RNA Library Prep Kit (NEB # E7760L). Poly-A selection (NEBNext Poly (A ...
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bioRxiv - Molecular Biology 2023Quote: Sequencing libraries were prepared using the NEBNext Ultra II DNA library prep kit according to the manufacturer protocol (NEB). Sequencing libraries were pooled and analyzed on a NextSeq 550 platform using paired-end mode with 32 cycles on both ends ...
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bioRxiv - Microbiology 2023Quote: 20 ng of nucleic acid was enriched for viral genomic sequences using NEBNext Microbiome DNA Enrichment Kit (NEB, E2612) to reduce host genomic DNA contamination ...
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bioRxiv - Molecular Biology 2023Quote: ... The pNH605-RIM11 was amplified by making use of the Q5® Site-Directed Mutagenesis Kit (New England Biolabs) following the instructions of the manufacturer to generate pNH605-RIM11 Y199F ...
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bioRxiv - Molecular Biology 2023Quote: ... and cDNA libraries were prepared using NEBNext® UltraII RNA library preparation kit (NEB, New England Biolabs, MA, USA) using the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and cDNA libraries were prepared using NEBNext® UltraII RNA library preparation kit (NEB, New England Biolabs, MA, USA) using the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA fragments were then constructed into libraries using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645). 100M reads/read pairs per library were requested ...
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bioRxiv - Bioengineering 2023Quote: ... 20 ng of each integrase amplicon was used per reaction using PURExpress® In Vitro Protein Synthesis Kit (NEB) in a total volume of 9 µl ...
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bioRxiv - Evolutionary Biology 2023Quote: ... using the Monarch® HMW DNA Extraction Kit for Tissue (Cat. No. T3060S, New England BioLabs Inc., MA, USA). DNA from resequencing samples was isolated using either a modified protocol for the DNeasy Blood and Tissue Kit (Cat ...
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bioRxiv - Developmental Biology 2023Quote: ... and RNA libraries were prepared using NEBNext® Ultra TM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: ... Non-β-lactamase genes used as controls were cloned into the pBAD18 using the Gibson Assembly Cloning Kit (NEB). pBAD (RBS)_fwd/rev primer pairs were used to amplify the plasmid backbone ...
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bioRxiv - Microbiology 2023Quote: cDNA was generated starting from 150 ng of RNA using ProtoScript® II First Strand cDNA Synthesis Kit (NEB) using random hexamers in agreement with the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2023Quote: ... the cDNA libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). The resulting libraries were measured using the Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... mRNA was reverse transcribed into cDNA using ProtoScript® II First Strand cDNA Synthesis Kit (BioLabs, Ipswich, MA, USA). Ribosomal Protein L32 (RPL32 ...
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bioRxiv - Cancer Biology 2023Quote: Kinase-dead mutant (K37R) of Nek2A was derived from Nek2A in pJP1563 using Q5 Site-Directed Mutagenesis Kit (NEB) following the instructions of manufacturer ...
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bioRxiv - Plant Biology 2023Quote: ... and each individual library was barcoded using the NEBNext® Multiplex Oligos for Illumina® kit (New England Biolabs).
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bioRxiv - Developmental Biology 2024Quote: ... NEBNext UltraTM II Directional RNA Library Prep Kit for Illumina and NEBNext UltraTM II DNA Library Prep Kit for Illumina according to the manufacturer’s instructions (New England Biolabs). Libraries were sequenced on a NextSeq2K (Illumina ...
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bioRxiv - Evolutionary Biology 2024Quote: ... in conjunction with the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB, #E7760) and the NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Neuroscience 2023Quote: ... The region of interest was amplified using Phusion® High-Fidelity PCR Kit (New England Biolabs, Cat. No. E0553S). The PCR product was purified with QIAquick PCR Purification Kit (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... deposited by Bischof and Basler) at the EcoRI restriction site by NEBuilder HiFi DNA Assembly Cloning Kit (NEB #E5520S). The pUASTattB-Gs2 vector was injected to y1 w67c23 ...
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bioRxiv - Neuroscience 2023Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s instruction ...
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bioRxiv - Synthetic Biology 2023Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra RNA library Prep Kit for Illumina per manufacturer’s instructions (New England Biolabs). mRNAs were enriched with Oligo(dT ...
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bioRxiv - Neuroscience 2023Quote: ... RNA libraries were prepared using an established protocol with NEBNExt Ultra II Directional Library Prep Kit (NEB, Lynn, MA). Each library was made with one of the TruSeq barcode index sequences and the Illumina sequencing done by Medgenome (Foster City ...
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bioRxiv - Developmental Biology 2023Quote: ... mixed in a ratio 1:3 (insert:backbone plasmid) and ligated with T4 ligase according to manufacturer’s instructions (Quick Ligation kit, NEB M2200). The ligation reaction was transformed into competent TOP-10 bacteria and plated on agarose plates with Ampicillin ...
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bioRxiv - Cancer Biology 2023Quote: ... and PCR enrichment were performed sequentially using the NEBNext Ultra™ II DNA Library Prep Kit for Illumina (NEB). Lastly ...
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bioRxiv - Biochemistry 2023Quote: Mutant variants of the mS29-containing construct were generated using the Q5 site-directed mutagenesis kit (New England Biolabs). Mutagenesis primers were designed using the NEBaseChanger tool ...
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bioRxiv - Cell Biology 2023Quote: ... The RNA-Seq library was prepared by using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) and sequenced with the NextSeq 500/550 sequencing platform (performed at the NYUAD Sequencing Center) ...
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bioRxiv - Plant Biology 2024Quote: ... In-frame deletions and substitutions were generated using a Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Hitchen UK) using the primers listed in Table S1 ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was synthesized from 1 µg total RNA using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB) with d(T)23VN primer.
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bioRxiv - Plant Biology 2024Quote: ... The Illumina cDNA library was prepared using a NEBNext Ultra II RNA Library Prep Kit (NEB, Ipswich, MA, USA) according to the standard experimental protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Adapter ligation and library preparation were carried out using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB), and adapter ligation was checked using qPCR ...
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bioRxiv - Physiology 2024Quote: ... Libraries were constructed from polyA-selected RNA using a NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs) and sequenced on Illumina HiSeq2500 instrument ...
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bioRxiv - Microbiology 2024Quote: ... Metagenomic sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep kit (New England Biolabs, MA, USA) with a targeted insert size of 350-400bp and Dual Index multiplex oligos ...
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bioRxiv - Neuroscience 2024Quote: ... and libraries for Illumina sequencing were prepared using the NEBNext Ultra II RNA kit (NEB, Ornat, Rehovot, Israel, #E7770L). Quantification of the library was performed using dsDNA HS assay kit and Qubit (Molecular Probes ...
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bioRxiv - Microbiology 2023Quote: ... The complementation plasmid was constructed by Gibson assembly using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). In brief ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were purified with DNA Spin and Concentrate kit (Zymo Research, Irvine, CA, D4013 or NEB Monarch, T1030) following manufacturer instructions or gel-purified from kit (Zymo Research) ...
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bioRxiv - Pathology 2024Quote: ... Library preparation was completed on ribo-depleted RNA using the NEBNext Ultra Directional RNA Library prep kit (NEB, #E7420L) using 8 cycles of PCR amplification and indexed using single (i7 ...
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bioRxiv - Developmental Biology 2024Quote: ... Deletions of these constructs were constructed using the Q5 site-directed mutagenesis kit according to the manufacturer protocol (NEB). For construction of pMT-ed:Flag:vhh4 ...
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bioRxiv - Developmental Biology 2024Quote: ... The DNA-Seq libraries were prepared using the NEBNext DNA library preparation kit according to the manufacturer’s instructions (E6040, New England Biolabs) and the libraries were sequenced on an Illumina HiSeq 4000 instrument ...
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bioRxiv - Genomics 2024Quote: ... RNA-seq libraries were constructed using the NEBNext® Ultra™ RNA Library Prep Kit (NEB, Ipswich, MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... IVT was performed on the purified PCR product using a HiScribe T7 High yield RNA Synthesis Kit (NEB, E2040S) and purified using a Monarch RNA Cleanup Kit (NEB ...
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bioRxiv - Genomics 2024Quote: ... RNA sequencing libraries were prepared using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB) according to the recommended protocol using 1 ng RNA as input material ...
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bioRxiv - Microbiology 2024Quote: ... Mutations M1 (CAGGTGcAac) and M2 (CAGGTGTACA) in pX_Luc were introduced using Q5 site-directed mutagenesis kit (New England Biolabs). Plasmids expressing DYRK1A or DYRK1A_KR under the control of the CMV promoter were previously described [29].
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bioRxiv - Microbiology 2024Quote: ... illumina sequencing libraries were prepared with a NEBNext®Ultra™DNA Library preparation kit (New England Biolabs, USA) of 500 mg DNA samples from each sample ...
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bioRxiv - Synthetic Biology 2024Quote: ... then inserted the library cassette into the digested pMPRA1 backbone using the NEBuilder HiFi DNA Assembly kit (NEB E2621). This intermediate plasmid was purified with Kapa Pure Beads (Roche KK8002) ...
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bioRxiv - Cancer Biology 2024Quote: ... and PCR enrichment were performed sequentially using the NEBNext Ultra™ II DNA Library Prep Kit for Illumina (NEB). Finally ...
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bioRxiv - Bioengineering 2024Quote: ... The cDNA for catalytically inactive E447D mutant was generated through mutation of StcEΔ35 using the Q5 Site-Directed Mutagenesis Kit (Cat # E0552S, New England Biolabs) with primers 5’-TCAGTCATGACGTTGGTCATAATTATG-3’ and 5’-ACTCATTCCCCAATGTGG-3’ ...
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bioRxiv - Developmental Biology 2024Quote: ... The RNA-sequencing (RNA-seq) libraries were generated using the NEBNext Ultra Directional RNA Library Prep Kit (NEB, E7420L), The yield of cDNA libraries was measured using the Qubit dsDNA HS Assay Kit ...