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Citations for New England Biolabs :
6251 - 6300 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... The sgRNA was synthesized using the NEB HiScribe T7 Fast and Efficient RNA Synthesis Kit (NEB E2050S, MA, USA). After mixing 5μM~10μM sgRNAs of bmp6a and bmp6b and 5μM Cas9 protein (M0646 ...
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bioRxiv - Genetics 2022Quote: ... exon duplication and exon deletion mutants were generated by site-directed mutagenesis PCR following the manufacturer’s instructions of the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). To generate the plasmids for in vitro transcription/translation of the fusion proteins SmMADS3-GFP and CRM3-GFP ...
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bioRxiv - Immunology 2023Quote: pals-17::gfp and pals-20::wrmScarlet translational reporter constructs were assembled using NEBuilder HiFi kit (New England Biolabs) by fusing PCR products and gene fragments ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: DNA for WGS was prepared by PCR-free library preparation according to the NEBNext DNA Ultra II kit (NEB). Sequencing was performed by Illumina NextSeq 2000 P3 150PE.
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bioRxiv - Immunology 2023Quote: 1 μg of total RNA was subjected to rRNA depletion using the NEBNext rRNA Depletion Kit (New England Biolabs), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... of ATG13 and ATG13.11-198 referred to as ATG13.1HORMA were introduced into linearised pBMN-HA-C1 using Gibson Cloning kit (New England Biolabs) to create pBMN-HA-ATG13 and ATG13.1HORMA (Addgene #186223 and #18622 respectively) ...
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bioRxiv - Molecular Biology 2023Quote: DNA templates were transcribed and translated in vitro using a custom PURExpress ΔRF-123 ΔRibosome Kit (New England Biolabs) and a final concentration of 2.2 μM ribosomes ...
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bioRxiv - Biochemistry 2023Quote: ... Sequencing libraries were prepared using NEBNext Ultra II RNA library prep kit using the manufacturer’s instructions (NEB, Ipswich, MA). Sequencing libraries were validated on the Agilent TapeStation and quantified using Qubit 2.0 Fluorometer as well as via quantitative PCR (KAPA Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 pmol of TP_MAAAPQKCAAA* mRNA (see “Toeprinting assays” section) and components of the PURExpress ΔRibosomes Kit (New England Biolabs). The reaction was incubated at 37ºC for 20 min and then diluted in 50 mM Hepes KOH pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA expression was determined using SYBR green Luna Universal One-step RT-PCR kit (New England Biolabs, Cat #E3005L) with a BioRad CFX96 thermocycler ...
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bioRxiv - Plant Biology 2023Quote: ... Poly-A enriched libraries were prepared using the NEBNext UltraTM RNA Library Prep Kit (New England Biolabs, Ipswich, USA) for Illumina ...
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bioRxiv - Physiology 2023Quote: ... cDNA was generated from 500ng of RNA using the LunaScript RT Supermix kit (New England Biolabs, Cat. No. E3010L) according to the manufacturer’s directions ...
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bioRxiv - Systems Biology 2023Quote: ... metatranscriptomic libraries were constructed according to the NEBNext® UltraTM RNA Library Prep Kit (Illumina, New England Biolabs, MA) and sequenced on the Illumina Hiseq 2500 platform (Illumina ...
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bioRxiv - Systems Biology 2023Quote: ... Dual-indexed sequencing libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) with 10 ng or 10 uL of input DNA (whichever was lower volume) ...
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bioRxiv - Genomics 2023Quote: ... Preparing the MLI1 DNA library was performed using NEBNext Ultra II DNA Library Prep kit (New England Biolabs, USA) and sequencing on an Illumina MiSeq with paired-end 300 bp reads at the “Molecular and Cellular Biology” core facility of the IMCB SB RAS (Novosibirsk ...
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bioRxiv - Cell Biology 2023Quote: ... APOE3-C-mEm ΔSS were created from APOE3-mEm using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554S). The N-terminal domain constructs consist of residues 19-209 with or without residues 1-18 of the N-terminal signal peptide ...
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bioRxiv - Developmental Biology 2023Quote: ... Eluted DNA fragments were amplified using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ChIP sequencing libraries were prepared using NEBNext® Ultra™ II DNA Library Prep Kit reagents (NEB, Ipswitch, MA) according to the procedure recommended by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... which was subsequently cloned into linearized pUHE-21 (106) using NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs). Construct was verified by Sanger DNA sequencing with primers 146 and 156.
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bioRxiv - Microbiology 2023Quote: The pGEX-6P-PrkA1-338 plasmid (Table 2) was constructed by a two-part ligation (NEB Quick Ligation kit) of BamHI- and NotI-linearized pGEX-6P and PCR-generated prkA1-338 amplified with primers AS21 and AS81 (Table 3 ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 µL 100 µM dNTPs, 0.3 µL 50 mg/mL BSA, 6 µL blunting enzyme mix from quick blunting kit, NEB). Prior to ligation ...
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bioRxiv - Cell Biology 2023Quote: ... 200ng of cleaned DNA was used as an input for conversion using a Enzymatic Methyl-seq Kit (NEB E7120S) according to the manufacturer’s recommended protocol ...
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bioRxiv - Genetics 2023Quote: Whole genome DNA methylation sequencing was performed using the NEBNext Enzymatic Methyl-seq (EM-seq) Kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... followed by preparation of RNA libraries using the NEBNext Ultra RNA Prep kit (NEB #E7530) (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Neuroscience 2023Quote: ... followed by preparation of RNA libraries using the NEBNext Ultra RNA Prep kit (NEB #E7530) (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Neuroscience 2023Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (#E7530L, NEB, USA) following the manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Plant Biology 2023Quote: ... ChIP DNA libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and RNA-Seq libraries were generated using the NEBNext® Ultra II Directional RNA Library Prep Kit (NEB # E7760L). Two biological replicates were sequenced using the Illumina NextSeq 550 platform to generate 150 bp PE reads.
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bioRxiv - Plant Biology 2023Quote: ... This was followed by two sets of enzymatic conversion steps using the Enzymatic Methyl-seq kit (NEB, Cat# E7120L) to differentiate cytosines from 5mC and 5hmC ...
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bioRxiv - Microbiology 2023Quote: ... and DNA libraries were prepared using the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs, NEB). DNA purifications and size selections were made using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... and DNA libraries were prepared using the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs, NEB). DNA purifications and size selections were made using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA-seq libraries were made using the NEBNext Ultra II Directional RNA Library Prep kit for Illumina (NEB, #E7760) according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2024Quote: ... Total RNA was then extracted using a Monarch® Total RNA Miniprep Kit (New England Biolabs, Whitby, ON, Canada) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... The cDNAs were transcribed in vitro to cRNAs using the HiScribe® T7 Arca mRNA Kit (New England BioLabs). Single mutations in Cx26 (W3A ...
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bioRxiv - Immunology 2024Quote: ... RNA sequencing libraries were produced by using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770L). Quantification of the library was performed using a dsDNA HS Assay Kit and Qubit (Molecular Probes ...
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bioRxiv - Microbiology 2024Quote: ... Purified RNA was depleted of ribosomal RNA (rRNA) using a NEBNext® rRNA Depletion Kit (Bacteria) (NEB, #E7850L/X) and library preparation was done using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... Illumina library was made from polyA RNA with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s manual74 ...
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bioRxiv - Plant Biology 2024Quote: ... Site-directed mutagenesis transgenic plants were generated by using the Q5 ® Site-Directed Mutagenesis Kit (New England Biolabs) in the pDONR221 vector and recombined with the above-mentioned promotor constructs into the pFR7m24GW vector using the Gateway Technology ...
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bioRxiv - Biochemistry 2024Quote: ... The activating Q163C/Q309C cysteine mutations were introduced into αMI-domain using the Q5 Site-Directed Mutagenesis Kit (NEB). The plasmids were transformed into either BL21(DE3 ...
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bioRxiv - Systems Biology 2024Quote: ... 5µl of RNA (roughly corresponding to 1µg) was reverse transcribed to cDNA using the LunaScript RT SuperMix kit - dye based qPCR detection (NEB) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... pLenti-PGK-ATP6V1H(Δ176-191)-FLAG-Puro was produced using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). M4P-EGFP-LC3B and pOGP were kind gifts from F ...
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bioRxiv - Microbiology 2023Quote: ... a KOZAK sequence was added in front of the TY1 region with the Q5 Site-Directed Mutagenesis Kit (NEB) using primers F167 and F168 ...
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bioRxiv - Microbiology 2023Quote: ... RNA sequencing library preparation was performed utilizing the NEBNext Ultra RNA Library Prep Kit for Illumina by following the manufacturer’s recommendations (NEB). Sequencing libraries were validated on the Agilent 2100 Bioanalyzer System (Agilent Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR product was cleaned and concentrated with the Zymo Research kit following the manufacturer’s protocol and then digested by DpnI (New England BioLabs) overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were prepared using 50ng RNA according to the manufacturer’s recommendation for the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) and sequenced on the Illumina NextSeq 500.
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bioRxiv - Microbiology 2023Quote: ... NEBNext® UltraTM II Non-Directional RNA Library Prep Kit for Illumina® (New England BioLabs Inc., MA, USA) was used for the remaining steps of library construction ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were generated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, UK) following manufactures’ protocol ...
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bioRxiv - Microbiology 2023Quote: ... Removal of rRNA from the samples was carried out using NEBNext Ultra directional RNA library prep kit (NEB, MA). Sequencing libraries were generated using the NEBNext Ultra RNA library prep kit for Illumina (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... Replacement of the interchangeable sgRNA was accomplished using a Q5 site-directed mutagenesis kit (Table S3) (New England BioLabs). The TgLaforin-HA tagging construct was generated by amplifying the 3’ end of the TgLaforin-HA construct generated for complementation (see generation of COMP line below and Tables S2 and S3 ...
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bioRxiv - Microbiology 2023Quote: ... Site-specific mutagenesis of efp was carried out with the Q5® Site-Directed Mutagenesis Kit (New England BioLabs) according to manufacturer’s instructions.