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Citations for New England Biolabs :
6251 - 6300 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... Plasmid DNA and total DNA was isolated from overnight cultures using Monarch® plasmid mini-prep kit (NEB, #T1010) and Monarch® genomic DNA purification kit (NEB ...
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bioRxiv - Immunology 2024Quote: ... Sample libraries were prepared using the NEBNext UltraTM II Directional RNA Library Prep Kit for Illumina (New England Biolabs) and cDNA libraries were sequenced using an Illumina HiSeq machine (2×50 bp sequence reads) ...
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bioRxiv - Immunology 2024Quote: ... Reverse transcription was then performed with 0,5-1 μg of RNA using ProtoScript II First Strand cDNA Synthesis Kit (NEB) and 3′-RACE CDS primer (Clontech) ...
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bioRxiv - Microbiology 2024Quote: ... Illumina sequencing libraries were built using the NEBNext Ultra II FS DNA Library Prep kit (New England Biolabs, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: Libraries of converted methylated DNA were prepared using the NEB Enzymatic Methyl-seq Kit (New England Biolabs Inc., USA) with the EM-seq Index Primers (New England Biolabs Inc. ...
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bioRxiv - Molecular Biology 2024Quote: ... pB-T-PAF-Clu-CC4 was obtained by mutagenesis using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). pB-T-PAF-Clu-DD1+TL1 was obtained by PCR amplification of pB-T-PAF-Clu-DD1 plasmid to delete the TL1 region and assembly with the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs).
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bioRxiv - Microbiology 2024Quote: ... The cDNA Library was constructed following the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) manual ...
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bioRxiv - Synthetic Biology 2024Quote: ... Amplicons from synthesized DNA fragments were purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs, Cat# T1030L), while amplicons from plasmids were purified using the Monarch Gel DNA Extraction Kit (New England Biolabs ...
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bioRxiv - Genomics 2024Quote: ... Each sample were subjected to one reaction of NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645). Deamination reactions were conducted as described before ...
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bioRxiv - Immunology 2024Quote: ... total RNA was isolated for the same individuals with the Monarch® Total RNA Miniprep Kit (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... For RT-qPCR 1µg of total RNA was used for first strand cDNA preparation by using ProtoScript II Reverse Transcriptase kit (NEB) followed by LUNA Universal qPCR kit (NEB) ...
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bioRxiv - Immunology 2024Quote: ... Library preparation was performed using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645S) as per the manufacturer’s recommendations and indexed using NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Immunology 2024Quote: ... An Illumina sequencing library was prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) following the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2024Quote: ... libraries were prepared for all samples using the NEB Next Ultra II Directional RNA Library Prep Kit (NEB, E7760S) according to manufacturer protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Library preparation was done using NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (NEB #E7645, #E7103). DNA was sequenced on Illumina HiSeq 2500 platform.
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bioRxiv - Molecular Biology 2024Quote: ... The purified DNA samples were then used for library preparation using NEBNext UltraII DNA Library prep kit (NEB, E7370L) and were sequenced at the iGE3 platform of the University of Geneva.
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bioRxiv - Molecular Biology 2024Quote: ... Library preparation was carried out on the purified DNA using NEBNext Ultra II DNA Library Prep Kit (NEB #E7645) and sequenced at 100 million reads per sample on a NovaSeq6000 using 75-nucleotide read length paired-end sequencing.
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bioRxiv - Neuroscience 2024Quote: ... 100ng input RNA was used for library preparation with the NEBNext Globin & rRNA depletion kit (New England Biolabs, E7750X) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs, Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... and multiplexed RNA-seq libraries were generated using the NEBNext Ultra Directional RNA library Prep Kit for Illumina (NEB). Libraries were sequenced (75 bp ...
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bioRxiv - Microbiology 2024Quote: ... DNA fragmentation was performed using the NEBNext® Ultra™ II FS DNA Library Prep Kit (New England Biolabs), an enzymatic fragmentation assay with an average fragment size of 380 bp ...
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bioRxiv - Microbiology 2024Quote: ... Samples were then treated with DNase I and cleaned with the Monarch RNA Cleanup Kit (NEB, Ipswich, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... a vector containing an N-terminal Thioredoxin-His10 tag using a Gibson Assembly® Cloning Kit (New England BioLabs). Plasmids were extracted and transformed into competent E ...
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bioRxiv - Genomics 2024Quote: ... Small RNA libraries were generated using the NEBnext small RNA library preparation kit according to instructions (New England BioLabs). small RNA high-throughput sequencing libraries were sequenced for 101 cycles using NovaSeq X Plus ...
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bioRxiv - Genetics 2024Quote: ... ChIP-seq libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) without size selection ...
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bioRxiv - Immunology 2024Quote: Site-directed mutants in this study were generated using a Q5 site directed mutagenesis kit according to the manufacturer’s instructions (NEB). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... EM-seq was performed with 100ng of purified genomic DNA using NEBNext® Enzymatic Methyl-seq Kit (NEB, E7120) and following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Digested oligos and vector were gel-purified with Qiagen gel purification kit and ligated by T4 DNA ligase (NEB). Ligate was transformed into Stbl3 competent bacteria (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: ... All connections between genes and linearized vectors were achieved by Gibson assembly (Gibson assembly kit, New England BioLabs, UK). The shell-(csoS2-NM) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Lettuce variant sequences were cloned into retron Eco2 plasmid using Q5® Site-Directed Mutagenesis Kit (New England Biolabs). Primer sequences and annealing temperatures were generated using the NEBaseChanger™ tool.
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bioRxiv - Synthetic Biology 2024Quote: ... RNA strands were transcribed in-house and column-purified using the Monarch® RNA Cleanup Kit (New England Biolabs). The concentration of oligonucleotides was quantified by UV-vis spectrophotometry using a Thermo Fisher Scientific NanoDrop 2000 Spectrophotometer ...
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bioRxiv - Synthetic Biology 2024Quote: ... and the amplified plasmid DNA was extracted using the Monarch® DNA Gel Extraction Kit (New England Biolabs, USA) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: All transcription templates were generated using PCR amplification (Phusion High-Fidelity PCR Kit, New England Biolabs, catalog no. E0553) of an oligo that includes a T7 RNAP promoter ...
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bioRxiv - Molecular Biology 2024Quote: ... All qPCRs were performed using the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA) and a Step One Plus qPCR machine (Life Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from bulk liver tissue using the Monarch total RNA miniprep kit (New England Biolabs, Ipswich, MA) after homogenization with steel beads using a TissueLyser LT bead mill (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: 50 ng of DNA template was transcribed using a HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) according to the manufacturer’s protocol but at one-quarter volume (5 µl) ...
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bioRxiv - Microbiology 2024Quote: ... part of the ONT Kit) to the RNA sample using a mix of 3μl NEBNext Quick Ligation Reaction Buffer (New England BioLabs), 0.5μl RNA CS (ONT Kit) ...
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Fluoroquinolone-Specific Resistance Trajectories in E. coli and their Dependence on the SOS-ResponsebioRxiv - Microbiology 2024Quote: ... Genomic DNA libraries were created according to the manufacturer’s protocol using NEBNext Ultra II FS DNA Library Prep kit for Illumina (New England BioLabs) in combination with NEBNext multiplex oligos for Illumina (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Neuroscience 2024Quote: The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ChIP-seq libraries were built using an NEBNext® Ultra™ II DNA Library Prep Kit (NEB #E7645S/L) with immunoprecipitated or input DNA ...
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bioRxiv - Microbiology 2024Quote: ... RIG-I and ISG15 were determined using Luna Universal One-Step RT-qPCR Kit according to the manufacturer’s instructions (New England Biolabs). The primer sequences are available upon request ...
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bioRxiv - Plant Biology 2024Quote: ... Synthesis of cDNA was conducted via ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, Ipswich MA, USA), using manufacturer’s instructions for reverse transcription of total RNA ...
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bioRxiv - Physiology 2024Quote: ... RNA-Seq libraries were constructed using either NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) or by Novogene RNA-seq service ...
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bioRxiv - Cell Biology 2024Quote: ... libraries were prepared with the NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs, E7765) in conjunction with the NEBNext® Poly(A ...
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bioRxiv - Plant Biology 2024Quote: ... end repair and adaptor ligation were performed following the manual of NEBNext Ultra II DNA Library Prep Kit (NEB). The ligated DNA was then affinity pulled-down with Dynabeads MyOne Streptavidin C1 beads (Invitrogen) ...
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bioRxiv - Biophysics 2024Quote: ... Total RNA was isolated using the SPLIT RNA Extraction Kit (Lexogen, Austria) and treated with DNase I (NEB, USA) to eliminate genomic DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNAs (gDNAs) were extracted using the Monarch HMW DNA extraction kit for Tissue from New England Biolabs (NEB) with the following modifications ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared using the NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs). sPOINT was performed in the same manner with the following difference ...
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bioRxiv - Neuroscience 2024Quote: ... The synthesised sgRNAs was cleaned up with the Monarch® RNA Cleanup Kit (50μg, New England BioLabs, USA, #T2040L), following the instructions ...
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bioRxiv - Molecular Biology 2024Quote: CFTR-Δexon deletion plasmids were constructed from the synthetic CFTR high codon adaption index (HCAI) into the pcDNA3.1/Neo(+) vector using the Q5 Site-Directed Mutagenesis Kit (NEB) (Table S1 ...