Labshake search
Citations for New England Biolabs :
6301 - 6350 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... The poly A enriched samples were then used as input in the NEBnext Ultra II RNA kit (NEB, USA), and processed following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Sequencing libraries for ChIP and input samples were prepared with NEBNext Ultra II DNA library prep kit (NEB, E7645) reagents according to the manufacturer’s protocol on beads suspended in 25 μL TT (10 mM Tris/HCl pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... genes were synthesized (Twist Biosciences) and cloned into the expression vector pCDNA3.1 using NEBuilder HiFi DNA Assembly Kit (New England Biolabs). Constructs were verified by sequencing ...
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bioRxiv - Plant Biology 2024Quote: ... The NEBNext Ultra II Directional RNA Library Prep Kit was used to construct PolyA libraries according to the manufacture’s protocol (New England Biolabs). The libraries were sequenced with PE150 format by Admera Health (South Plainfield ...
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bioRxiv - Genomics 2024Quote: The libraries were generated by using the NEBNext® Ultra™ II DNA Library Prep Kit (NEB, Cat.N: E7103S), adhering to the manufacturer’s guidelines ...
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bioRxiv - Genomics 2024Quote: Skin biopsies were cut into small pieces and processed with the Monarch Genomic DNA Purification Kit (NEB, Cat. T3010S) or DNeasy blood and tissue kit (Qiagen ...
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bioRxiv - Immunology 2024Quote: ... and subsequently subjected to in vitro transcription (IVT) with the HiScribe T7 high yield RNA synthesis kit (NEB, E2040S). Following ExoSAP-IT treatment (Affymetrix ...
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bioRxiv - Biochemistry 2024Quote: ... open reading frame cloned in pDNA3.1 expression plasmid were each mutated to alanine using Q5 site-directed mutagenesis kit (NEB) and appropriate primers obtained from Integrated DNA technologies (Table 1) ...
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bioRxiv - Synthetic Biology 2024Quote: The genomic DNA was extracted from the WNT-BC1 INSCRIBE cells using the Genomic DNA Purification Kit (NEB, T3010) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: The 9N_VRA3 adapter oligonucleotide (0139, Supplementary Table 2) was pre-adenylated with the 5’ DNA Adenylation Kit (New England Biolabs) using the following protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were generated using the NEBNext Ultra II Kit following the manufacturer’s instructions with the following parameters: the input DNA was fragmented (FS DNA Module, NEB) for 40mins at 37C and 8 cycles for the library construction PCR ...
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bioRxiv - Genomics 2024Quote: ... Library preparation was continued using the NEB NEBNext® UltraTM II DNA Library Prep Kit for Illumina (NEB #E7645S) starting at “Step 4 ...
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bioRxiv - Neuroscience 2024Quote: ... RNA-seq libraries were prepared using NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770, New England BioLabs). End repair was performed using NEBNext Ultra II End Repair/dA-Tailing Module (E7546L ...
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bioRxiv - Genomics 2024Quote: ... DNA ends were FFPE repaired and end-prepped/dA-tailed using the NEBNext FFPE DNA Repair kit (M6630, NEB) and the NEBNext Ultra II End-Repair/dA-tailing Module (E7546 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were then blunted and 3’ A tailed using the Quick Blunting Kit and Klenow Fragment (New England Biolabs). TruSeq indexed and universal adapters (Integrated DNA Technologies ...
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bioRxiv - Genetics 2024Quote: ... For RT-qPCR 1µg of total RNA was used for first strand cDNA preparation by using ProtoScript II Reverse Transcriptase kit (NEB) followed by LUNA Universal qPCR kit (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... NL) and further prepared for sequencing using the NEBNext Ultra II Directional RNA library preparation kit (New England Biolabs). Library concentrations were measured using Qubit HS DNA assay and library DNA size estimated using TapeStation with D1000 ScreenTape ...
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bioRxiv - Synthetic Biology 2024Quote: ... while the digested pM probe pool was purified using the DNA Gel Extraction Kit (New England Biolabs, Cat# T1020L) to isolate the insert fragment (tPT2A-fp) ...
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bioRxiv - Biochemistry 2024Quote: ... Δ419-427 and Δ591-627 were obtained from the pETM11-SUMO3_ECT2 recombinant plasmid by site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, BioLabs) using the primers listed in Suppl ...
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bioRxiv - Biochemistry 2024Quote: ... capsulatus anfH promoter via Golden Gate assembly50 using NEBridge Golden Gate Assembly Kit (New England Biolabs, Ipswich, Massachusetts, USA) into a broad-host range plasmid pOGG02451 (pMM0165) ...
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bioRxiv - Biophysics 2024Quote: ... The final RNA template was synthesized using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (E2050S, NEB), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... The PCR fragments were purified using Monarch® PCR & DNA Cleanup Kit (5 μg) from New England Biolabs (NEB) Cat#T1030L ...
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bioRxiv - Bioengineering 2024Quote: ... 5’-AGCTACCGACAACAACGTGT-3’ and Cap9_ Kpn/Age_Rev: 5’-AGAAGGGTGAAAGTTGCCGT-3’ and Phusion High-Fidelity PCR kit (New England Biolabs). The amplicon was gel purified digested with KpnI ...
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bioRxiv - Bioengineering 2024Quote: ... Gene fragments were cloned using Golden Gate cloning with BsmBI-v2 golden gate assembly kit (New England Biolabs; E1602S). Resulting plasmids were transformed into Dh5⍺ competent E ...
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bioRxiv - Cancer Biology 2024Quote: ... The WI290>C substitution was generated in the pMSCV-FGFR2wt construct using the Q5 site-directed mutagenesis kit (NEB). pMSCV-Blasticidin (empty vector ...
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bioRxiv - Bioengineering 2024Quote: ... and cDNA libraries for sequencing were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760 ...
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bioRxiv - Cancer Biology 2024Quote: ... One hundred ng of DNA template was used to generate PCR using Phusion® High-Fidelity PCR kit (NEB). PCR was performed by using DNA Engine Tetrad® Thermal cycler (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... RNA extraction was then performed using the Monarch Total RNA Mini Prep Kit (T2010, New England Biolabs, MA, USA) with on-column DNase-I digest as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... RNA sequencing libraries were prepared using NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... mRNA enrichment and cDNA library preparation was performed using the stranded (polyA) mRNA library preparation kit (New England Biolabs), and paired-end 150 base pair sequencing was performed on the Illumina NovaSeq 6000 with an average of 50 million reads per sample ...
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bioRxiv - Biochemistry 2024Quote: ... The WT- DHFR DNA template for protein expression was obtained from the PURExpress in vitro protein synthesis kit (NEB). The P105TAG-DHFR DNA template containing a stop codon at position 105 was mutated from the DHFR-WT DNA using the Q5 site-directed mutagenesis kit (NEB).
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Structure of the SMYD2-PARP1 Complex Reveals Both Productive and Allosteric Modes of Peptide BindingbioRxiv - Biochemistry 2024Quote: ... SMYD2-F184A and SMYD2-L351A/W356A mutants were prepared using the Phusion Site-directed Mutagenesis Kit (New England Biolabs) according to the manufacturer’s instructions (29) ...
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bioRxiv - Biochemistry 2024Quote: ... the free DNA fragments were purified using a Monarch PCR & DNA Cleanup Kit (5 μg) (New England Biolabs, #T1030S) and quantified ...
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bioRxiv - Cancer Biology 2024Quote: ... ChIP-seq libraries were prepared using NEBNext Ultra II DNA Library preparation Kit following the manufacturer’s instructions (NEB, E7645). Libraries were amplified for 9 cycles using Q5 Hot Start High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... and GFP:RAB32DN (T39N) were generated by site-directed mutagenesis using the Q5 site-directed mutagenesis kit (E0554; New England Biolabs). ALS2 with a single C-terminal HA tag was synthesized by Twist Biosciences and inserted into the NotI/NheI sites of the pTwist CMVPuro plasmid ...
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bioRxiv - Cancer Biology 2024Quote: ... Knockout was confirmed in mouse genomic DNA extracted using Monarch Genomic DNA Purification kit (New England Biolabs, Ref T3010S) followed by PCR amplification of the surrounding ∼500bp next to the sgRNA target site ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA-seq libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB E7760) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... ChIP DNA library was made using NEBNext® Ultra™ II DNA Library Prep Kit (New England Biolabs, #E7645S).
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bioRxiv - Cancer Biology 2024Quote: ... and purified DNA fragments were used for library preparation with NEBNext DNA Library Prep Kit for Illumina (NEB E7645). Libraries were then sequenced (paired-end ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was in vitro transcribed from the clean PCR product using HiScribe T7 High Yield RNA Synthesis kit (NEB), followed by TurboDNase treatment (Invitrogen) ...
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bioRxiv - Genetics 2024Quote: 7.5 µL of the samples were mixed with 2.5 µL unique Native Barcode (NB01-96) from the Native Barcoding kit (ONT, SQK-NBD114.96) and 10µL Blunt/TA Ligase Master Mix (NEB, M0367), the sample mix flicked and spun down ...
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bioRxiv - Genetics 2024Quote: ... Linearized destination vectors were gel purified using 0.7% agarose gels and extracted with the Monarch DNA Gel Extraction Kit (New England Biolabs), before further purification by isopropanol precipitation.
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bioRxiv - Cell Biology 2024Quote: ... The sgRNA was synthesized using HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, Cat. No. E2050S).
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bioRxiv - Evolutionary Biology 2024Quote: ... Libraries were end repaired and ligated to adaptors using NEBNext Ultra II library prep kit (New England Biolabs E7645). Next ...
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A Newborn F-box Gene Blocks Gene Flow by Selectively Degrading Phosphoglucomutase in Species HybridsbioRxiv - Evolutionary Biology 2024Quote: ... Double-stranded RNAs (dsRNAs) were in vitro transcribed using the HiScribe T7 Quick High Yield RNA Synthesis kit (NEB) as described (60) ...
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bioRxiv - Genomics 2024Quote: Library prep kit(s) (e.g., EpiCypher 14-1001 or 14-1002 [each provides different 48x multiplex barcode sets] or NEB NEBNext Ultra II DNA Library Kit E7645 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... cDNA libraries were prepared from RNA samples using the NEBNext Ultra Directional RNA Library Prep Kit (Illumina compatible, NEB, #E7420L following the kit protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... RNA extraction was performed by OzOmics (Melbourne, Australia) using a Monarch® Total RNA Miniprep Kit (New England BioLabs). Library preparation was performed by AGRF (Melbourne ...
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bioRxiv - Evolutionary Biology 2024Quote: ... performed using the NEBNext Ultra™ II FS DNA Library Prep Kit for Illumina (New England Biolabs, MA, USA) following the protocol provided by the manufacturer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was reverse transcribed into cDNA using the LunaScript RT Master Mix Kit and d(T)23VN primers according to manufacturer’s instructions (New England Biolabs). Negative controls lacking the reverse transcriptase (RT ...