Labshake search
Citations for New England Biolabs :
6051 - 6100 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... In vitro transcription of sgRNAs was performed using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB). Then ...
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PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiationbioRxiv - Developmental Biology 2023Quote: ... Libraries were constructed using NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (NEB, E6420S) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Sequencing libraries were generated using the NEBNext Ultra™ DNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) following the manufacturer’s recommendations ...
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bioRxiv - Genetics 2023Quote: Libraries in this paper were prepared using NEB’s rRNA Depletion Kit (Bacteria) for rRNA removal with beads (NEB #E7860). Post rRNA removal samples were prepared for either Illumina or Singular Sequencing using NEBNext Ultra II RNA Library Prep Kit for Illumina with Beads (NEB#E7775) ...
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bioRxiv - Genomics 2023Quote: ... Linearized destination vectors were gel purified using 0.7% agarose gels and extracted with the Monarch DNA Gel Extraction Kit (New England Biolabs), before further purification by isopropanol precipitation.
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bioRxiv - Genomics 2023Quote: ... The remaining thirteen samples had paired-end sequencing libraries constructed according to the manufacturer’s specifications for the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB). Three samples (RMF031 ...
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bioRxiv - Genomics 2023Quote: Sample DNA concentrations were quantified using Qubit and libraries prepared using NEBNext Ultra II DNA library preparation kit (NEB) according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Developmental Biology 2023Quote: ... About 1ng DNA was used for library construction using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB). Sequencing (2× 150bp ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were prepared using the NEB Ultra II Directional RNA Library Prep Kit (New England BioLabs, Ipswich, United States), and all samples were run on one lane of the NovaSeq6000 S4 v1.5 (Illumina ...
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bioRxiv - Genomics 2023Quote: ... or 100 cGy) was processed for bisulfite sequencing library construction using NEBNext Ultra II DNA Library Prep Kit (NEB) along with methylated and indexed NEXTflex Bisulfite-seq Barcodes (Bio scientific) ...
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bioRxiv - Genomics 2023Quote: ... Total (“steady-state”) RNA was converted to sequencing libraries using NEB polyA capture kit (product no) followed by NEB Ultra directional II RNA-seq library kits ...
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bioRxiv - Cancer Biology 2023Quote: ... An RNA-seq library was prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Isotope labeled firefly luciferase (Fluc) mRNA was synthesized by HiScribe T7 High Yield RNA Synthesis Kit (E2040S, NEB, Ipswich) using stable heavy isotopes of ATP and CTP (NLM-3987-CA and CNLM-4267-CA-20 ...
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bioRxiv - Genetics 2024Quote: DNA from the VIU and MBP breeding program samples was extracted using the Monarch Genomic DNA Purification kit (NEB) using the enzymatic cleanup tissue extraction protocol with proteinase K and RNase A and quantified using a BioSpectrometer (Eppendorf) ...
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bioRxiv - Cell Biology 2023Quote: ... 200ng of cleaned DNA was used as an input for conversion using a Enzymatic Methyl-seq Kit (NEB E7120S) according to the manufacturer’s recommended protocol ...
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bioRxiv - Genetics 2023Quote: Whole genome DNA methylation sequencing was performed using the NEBNext Enzymatic Methyl-seq (EM-seq) Kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... followed by preparation of RNA libraries using the NEBNext Ultra RNA Prep kit (NEB #E7530) (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Neuroscience 2023Quote: ... followed by preparation of RNA libraries using the NEBNext Ultra RNA Prep kit (NEB #E7530) (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Plant Biology 2023Quote: ... ChIP DNA libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and RNA-Seq libraries were generated using the NEBNext® Ultra II Directional RNA Library Prep Kit (NEB # E7760L). Two biological replicates were sequenced using the Illumina NextSeq 550 platform to generate 150 bp PE reads.
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bioRxiv - Plant Biology 2023Quote: ... This was followed by two sets of enzymatic conversion steps using the Enzymatic Methyl-seq kit (NEB, Cat# E7120L) to differentiate cytosines from 5mC and 5hmC ...
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bioRxiv - Microbiology 2023Quote: ... and DNA libraries were prepared using the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs, NEB). DNA purifications and size selections were made using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... and DNA libraries were prepared using the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs, NEB). DNA purifications and size selections were made using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA-seq libraries were made using the NEBNext Ultra II Directional RNA Library Prep kit for Illumina (NEB, #E7760) according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2024Quote: ... Total RNA was then extracted using a Monarch® Total RNA Miniprep Kit (New England Biolabs, Whitby, ON, Canada) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... The cDNAs were transcribed in vitro to cRNAs using the HiScribe® T7 Arca mRNA Kit (New England BioLabs). Single mutations in Cx26 (W3A ...
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bioRxiv - Immunology 2024Quote: ... RNA sequencing libraries were produced by using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770L). Quantification of the library was performed using a dsDNA HS Assay Kit and Qubit (Molecular Probes ...
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bioRxiv - Microbiology 2024Quote: ... Purified RNA was depleted of ribosomal RNA (rRNA) using a NEBNext® rRNA Depletion Kit (Bacteria) (NEB, #E7850L/X) and library preparation was done using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... Illumina library was made from polyA RNA with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s manual74 ...
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bioRxiv - Plant Biology 2024Quote: ... Site-directed mutagenesis transgenic plants were generated by using the Q5 ® Site-Directed Mutagenesis Kit (New England Biolabs) in the pDONR221 vector and recombined with the above-mentioned promotor constructs into the pFR7m24GW vector using the Gateway Technology ...
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bioRxiv - Biochemistry 2024Quote: ... The activating Q163C/Q309C cysteine mutations were introduced into αMI-domain using the Q5 Site-Directed Mutagenesis Kit (NEB). The plasmids were transformed into either BL21(DE3 ...
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bioRxiv - Systems Biology 2024Quote: ... 5µl of RNA (roughly corresponding to 1µg) was reverse transcribed to cDNA using the LunaScript RT SuperMix kit - dye based qPCR detection (NEB) following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... In vitro transcription (IVT) eactions were performed with HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs). Template DNA was degraded with TURBO DNase (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... pLenti-PGK-ATP6V1H(Δ176-191)-FLAG-Puro was produced using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). M4P-EGFP-LC3B and pOGP were kind gifts from F ...
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bioRxiv - Microbiology 2023Quote: ... a KOZAK sequence was added in front of the TY1 region with the Q5 Site-Directed Mutagenesis Kit (NEB) using primers F167 and F168 ...
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bioRxiv - Microbiology 2023Quote: ... RNA sequencing library preparation was performed utilizing the NEBNext Ultra RNA Library Prep Kit for Illumina by following the manufacturer’s recommendations (NEB). Sequencing libraries were validated on the Agilent 2100 Bioanalyzer System (Agilent Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR product was cleaned and concentrated with the Zymo Research kit following the manufacturer’s protocol and then digested by DpnI (New England BioLabs) overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were prepared using 50ng RNA according to the manufacturer’s recommendation for the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) and sequenced on the Illumina NextSeq 500.
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bioRxiv - Microbiology 2023Quote: ... NEBNext® UltraTM II Non-Directional RNA Library Prep Kit for Illumina® (New England BioLabs Inc., MA, USA) was used for the remaining steps of library construction ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were generated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, UK) following manufactures’ protocol ...
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bioRxiv - Microbiology 2023Quote: ... Removal of rRNA from the samples was carried out using NEBNext Ultra directional RNA library prep kit (NEB, MA). Sequencing libraries were generated using the NEBNext Ultra RNA library prep kit for Illumina (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... Site-specific mutagenesis of efp was carried out with the Q5® Site-Directed Mutagenesis Kit (New England BioLabs) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... pcDNA3.1-ATP6V1H(Δ176-191)-FLAG and pcDNA3.1-ATP6V1H(Δ176-193)-FLAG were generated using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). pENTR-ATP6V1H-FLAG was produced using the pENTR/D-TOPO Cloning Kit (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by strand-specific cDNA NGS library preparation (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, NEB). The size of the resulting library was controlled by use of a D1000 ScreenTape (Agilent 2200 TapeStation ...
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bioRxiv - Genomics 2023Quote: 500 nanograms of total RNA were used for NEBNext® Ultra II Directional RNA Library Prep Kit (NEB # E7760L). Poly-A selection (NEBNext Poly (A ...
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bioRxiv - Molecular Biology 2023Quote: Sequencing libraries were prepared using the NEBNext Ultra II DNA library prep kit according to the manufacturer protocol (NEB). Sequencing libraries were pooled and analyzed on a NextSeq 550 platform using paired-end mode with 32 cycles on both ends ...
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bioRxiv - Molecular Biology 2023Quote: ... T7 transcription was performed for 16 h and then RNA was purified using the Monarch RNA Cleanup Kit (NEB). In vitro cleavage was performed with purified recombinant ...
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bioRxiv - Microbiology 2023Quote: 20 ng of nucleic acid was enriched for viral genomic sequences using NEBNext Microbiome DNA Enrichment Kit (NEB, E2612) to reduce host genomic DNA contamination ...
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bioRxiv - Molecular Biology 2023Quote: ... The pNH605-RIM11 was amplified by making use of the Q5® Site-Directed Mutagenesis Kit (New England Biolabs) following the instructions of the manufacturer to generate pNH605-RIM11 Y199F ...
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bioRxiv - Molecular Biology 2023Quote: ... and cDNA libraries were prepared using NEBNext® UltraII RNA library preparation kit (NEB, New England Biolabs, MA, USA) using the manufacturer’s protocol ...