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Citations for New England Biolabs :
6451 - 6500 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... single point mutations were introduced to the respective wild-type plasmids by site-directed mutagenesis using the Q5 site-directed mutagenesis Kit (NEB). The used primers are listed in table 1.
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bioRxiv - Evolutionary Biology 2022Quote: ... library sequence and terminator was PCR amplified to serve as template for in vitro transcription (NEB HiScribe T7 kit, USA). The PUREfrex 2.0 system (GeneFrontier Corporation ...
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bioRxiv - Cell Biology 2022Quote: ... 1mg of RNA was used to generate sequencing libraries using NEBNext Ultra II Directional RNA library Prep Kit for Illumina (NEB) according manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2022Quote: ... Second strand cDNA synthesis was performed with 1X SSS buffer and SSS enzyme (NebNext mRNA second strand synthesis kit NEB; thermocycler program ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was extracted with a Sodium Dodecyl Sulfate (SDS) protocol from whole bodies and sequencing libraries were built with NEBNext DNA Library Prep Kits (New England Biolabs) by Novogene (Hong Kong).
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bioRxiv - Evolutionary Biology 2022Quote: All constructs encoding for the protein fragments that were used as the tagless analyte in SPR experiments were produced using the IMPACT protein purification kit (NEB) following the manufacturer’s guidelines for cloning and refolding from denaturing conditions ...
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bioRxiv - Immunology 2022Quote: ... the purification of PolyA containing mRNA molecules using poly-T oligo attached magnetic beads from 100ng total RNA (with the Magnetic mRNA Isolation Kit from NEB), a fragmentation using divalent cations under elevated temperature to obtain approximately 300bp pieces ...
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bioRxiv - Immunology 2022Quote: ... was amplified from the synthetic S gene and cloned into the EcoRI and HindIII digested pXC17.4 CHO expression vector with a secretion signal sequence using NEBuilder HiFi DNA assembly kit (New England Biolabs, E5520). Similarly ...
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bioRxiv - Genomics 2020Quote: Synthetic SARS-CoV-2 RNA templates were serially diluted and amplified by RT-LAMP using the WarmStart LAMP Kit (NEB). LAMP primers were added to a final concentration of 0.2µM for F3 and B3 ...
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bioRxiv - Genomics 2020Quote: Small RNA sequencing libraries were generated using the NEBNext Small RNA library Prep kit and NEBNext multiplex oligos for Illumina according to the manufacturer’s instructions (New England Biolabs, E7300). The final small RNA libraries were purified from 6% PAGE gel ...
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bioRxiv - Genomics 2020Quote: ... In vitro transcription was performed using HiScribe T7 high yield RNA synthesis kit according to the manufacturer’s protocol (NEB, E2040S). In vitro synthesized RNAs was treated with 10U RNAse-free DNAse I (ThermoFisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... using 6 µg input 3C DNA as previously described8 or using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). When using the Ultra II kit 3 µg 3C material was sonicated to 200 bp as previously described8 ...
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bioRxiv - Genomics 2020Quote: The lentiviral vector for expressing TIR1 (Lentiv4-EFsp-Puro-2A-TIR1-9Myc) was constructed using PCR and Gibson Assembly Cloning kit (New England Biolabs). The backbone was modified from lentiCRISPR v259 (a gift from Feng Zhang ...
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bioRxiv - Genomics 2020Quote: PCR3 was performed by subtracting 2-3 cycles from the Ct and using 2.5 uL of a mix of distinct indexed primers from the NEBNext Dual Index Kit for Illumina (New England Biolabs) using 22.5 uL PCR2 product in a 50 uL reaction volume (Q5 UltraII mastermix with EvaGreen (Biotium) ...
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bioRxiv - Genomics 2020Quote: ... High quality RNA was subjected to library preparation using NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB, USA). The libraries were sequenced on a HiSeq X Ten sequencer (Illumina) ...
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bioRxiv - Microbiology 2020Quote: ... Genes with internal restriction sites for any of these two enzymes were subcloned into the same vector using the HIFI DNA Assembly kit (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... and Illumina-sequencer-compatible libraries were constructed by using an NEBNext Ultra II DNA Library Prep Kit (New England Biolabs).
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bioRxiv - Genomics 2020Quote: The immunoprecipitated sample and the input were subjected to Nextera library preparation as described by the manufacturer (NEBNext Ultra II DNA library Prep Kit for Illumina, NEB). The quality and size of libraries and chromatin shearing fragments were checked by Agilent Bioanalyzer using Agilent DNA High Sensitivity Chips (Agilent Technologies) ...
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bioRxiv - Genomics 2020Quote: ... end-repaired material (quantified by Nanodrop measurement) was ligated to Illumina adaptors supplied in the NEB DNA-seq kit (NEB cat ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries for cChIPseq and Native cChIPseq were prepared from 5-10ng of ChIP and corresponding input DNA samples using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs), following manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2021Quote: ... Two reactions of 1-5μg DNA each were adaptor-ligated and indexed using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) and NEBNext Multiplex Oligos for Illumina Primer sets 1 and 2 (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: Sequencing libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB Cat. E7645S). For HA-Rap1 ChIP ...
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bioRxiv - Molecular Biology 2021Quote: Plasmid mutagenesis to create SARS-CoV-2 mutant genes was achieved using the Q5® Site-Directed Mutagenesis (SDM) Kit according to the manufacturer’s instructions (NEB) or by using Gibson assembly with mutations introduced into the complementary overhang regions of the primer sequences ...
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bioRxiv - Genomics 2020Quote: ... and used to produce barcoded RNA sequencing libraries using the NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs). Libraries were sequenced on Illumina NextSeq 500 ...
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bioRxiv - Genetics 2020Quote: ... The concentration and purity of genomic DNA was determined using a Nanodrop OneC (Thermo) and 500 ng of genomic DNA was bisulphite-converted using the EpiMark Bisulfite Conversion Kit (New England BioLabs) as per manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... the sequence of the lead SNP construct was too GC rich to be synthesised and so the corresponding region was PCR amplified from a major allele homozygote and site-directed mutagenesis was used to create the alternate allele constructs (Q5 Site-Directed Mutagenesis Kit, NEB – used according to manufacturer’s instructions) ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmid #1129 used to obtain transgenic fish line expressing LifeAct-frFAST fusion under the control of the cmlc2 enhancer28 was constructed using the NEBuilder assembly kit (New England BioLabs) in the pT22i vector for transgenesis previously used29.
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bioRxiv - Microbiology 2020Quote: Reverse transcription and amplification for figure 2 was performed using OneTaq One-Step RT-PCR Kit from NEB (cat. # E5315S). Both the OneTaq One-Step RT-PCR Kit and Luna Universal One-step RT-qPCR Kit (cat ...
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bioRxiv - Developmental Biology 2020Quote: ... a total amount of 2µg of RNA per sample was used to prepare poly(A+)-enriched cDNA libraries using the NEBNext Ultra RNA library Prep Kit for Illumina (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... was transcribed from a sgRNA-coding PCR product with a 5′ T7 promoter sequence using HiScibe T7 Quick High yield RNA Synthesis kit (NEB). The transcription was performed at 37 °C overnight and then purified by phenol ...
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bioRxiv - Developmental Biology 2020Quote: ... mRNA-seq libraries were prepared with the NEBNext Ultra RNA library preparation kit with poly(A) mRNA magnetic isolation module (NEB) essentially according to manufacturer protocols ...
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bioRxiv - Genetics 2020Quote: ... ChIP enrichment was determined by RT-qPCR (Supplementary Table 5) prior to addition of sequencing adaptors using NEBNext Ultra II DNA Library Prep kit for Illumina (New England Biolabs). ATAC-seq was performed as previously described76 ...
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bioRxiv - Genetics 2020Quote: ... all wells from a single plate were pooled and prepared for sequencing with the NEBNext Ultra II DNA Library Prep kit for Illumina (New England Biolabs). Plates were multiplexed and sequenced on the MiSeq platform (Illumina ...
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bioRxiv - Biochemistry 2021Quote: cRNA were synthesized in vitro from linearized DNA of EGFP-fused mTTYH constructs using HiScribe T7 ARCA mRNA Kit (New England Biolabs). 1–2 ng of cRNA were injected in Xenopus laevis oocytes and imaged after 18 hours of incubation at 18 °C ...
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bioRxiv - Microbiology 2021Quote: ... primers oJV5-6 were used to remove ackA from pJV204 using the Q5 site-directed mutagenesis kit (NEB CN# E0554S) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Resulting cell pellets were washed in 1x PBS and resuspended in 1x DNA/RNA protection reagent (Monarch Total RNA Miniprep Kit, NEB). Subsequently ...
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bioRxiv - Genetics 2020Quote: ... CUT&RUN dsDNA samples were quantified with a Qubit III and 5-10ng used as input for library preparation using the NEBNext Ultra II DNA Library Prep Kit for Illumina (#E7645S, NEB). Libraries were prepared using the following PCR program ...
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bioRxiv - Genetics 2021Quote: ... FAN1 variants were introduced in the pFASTBAC1-GST-FAN1-FLAG-His plasmid by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Confirmed by DNA sequencing ...
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bioRxiv - Genomics 2021Quote: Wild-type HAP1 samples were used for standard ChIP-seq library preparation with NEBNext Ultra II DNA Library Prep kit (New England Biolabs), followed by sequencing on NovaSeq 6000 ...
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bioRxiv - Genomics 2021Quote: ... Shotgun sequencing was performed using a PCR-free DNA library preparation (NEBNext Ultra II DNA Library Prep Kit, New England Biolabs). Libraries were paired end 150 bp sequenced with Illumina HiSeqX ...
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bioRxiv - Genomics 2021Quote: ... Libraries were purified with SPRI beads (0.9X) and quantified on a bioanalyzer and with NEB Illumina quant kit (NEB#E7630). HiC libraries were sequenced on a NextSeq500 either 150bp or 75bp paired-end reads.
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bioRxiv - Cancer Biology 2020Quote: The Flag-HA-USP18WT construct was purchased from Addgene (#22572) and the Flag-HA-USP18C64R/C65R construct was generated using the Q5® Site-Directed Mutagenesis Kit (NEB, E0554S) and the primers OL87 ...
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bioRxiv - Genomics 2021Quote: The RNA-seq libraries were prepared as follows: the rRNA was depleted from 0.5 µg of A549 total RNA using the NEBNext rRNA Depletion Kit (NEB E6310). The resulting rRNA-depleted RNA was used for library construction with the NEB ultra II directional RNA-seq kit (NEB E7760) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and prepared DNA-Seq libraries from 100 ng DNA using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs; 12 minutes enzymatic shearing ...
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bioRxiv - Genomics 2021Quote: ... or the NEBNext Ultra II DNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (both New England Biolabs).
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was synthesized using 200 ng total RNA with the ProtoScript® First Strand cDNA Synthesis kit (New England Biolabs) utilizing random hexamers ...
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bioRxiv - Genomics 2021Quote: ... RNA was extracted from adult prothoracic leg tissue tissue using Monarch Total RNA Mini Kit (New England Biolabs, Ipswich, MA). Paired-end RNA-Seq libraries were constructed with NEBNext Ultra II Directional RNA prep (New England Biolabs ...
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bioRxiv - Immunology 2021Quote: ... cDNA was generated using the SuperScript IV reverse transcriptase kit (TFS) and PCR carried out using Q5 High-Fidelity 2X Master Mix (New England Biolabs) as per manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: ... β-ENaC/SCNN1B (Hs00165722_m1) and γ-ENaC/SCNN1G (Hs00168918_m1) were performed with the Luna Universal Probe One-Step RT-qPCR Kit (NEB, E3006L). Expression of each gene was normalized to 18S (Hs99999901_s1) ...
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bioRxiv - Plant Biology 2020Quote: T-DNAs expressing four sgRNAs were assembled from BsaI-linearized vectors and three PCR fragments using an NEBuilder kit (E2621, New England Biolabs). Appropriate PCR fragments were generated in three- or four-primer reactions similar to the ones described above ...