Labshake search
Citations for New England Biolabs :
5951 - 6000 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and libraries were quantitated with qPCR (Bio-rad CFX96 Touch Real-Time PCR, NEB Library Quant Kit for Illumina). Libraries were normalized to 0.5 nM and pooled ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Antisense strand DNA was digested in the samples using the Uracil-Specific Excision Reagent Enzyme Kit (New England Biolabs), followed by a PCR to amplify the cDNA ...
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bioRxiv - Plant Biology 2022Quote: ... The small RNA libraries were prepared using the NEBNext Small RNA Sample Prep kit (New England Biolabs, Ipswich, MA). High throughput sequencing was performed with NovaSeq-6000 (Illumina ...
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bioRxiv - Plant Biology 2022Quote: ... followed by RNA-seq libraries construction using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB E7420). RNA libraries were sequenced on an Illumina NovaSeq 6000 platform (PE150 bp) ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting plasmid backbone of the linearized vector was run on the 0.8% agarose gel and purified by Monarch DNA Gel Extraction Kit (NEB). The oligos for the inserted sgRNA targeting sequences were synthesized with two overhangs compatible with the linearized vector and complementary to each other ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® was used (New England Biolabs, MA, USA). For sequencing ...
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bioRxiv - Plant Biology 2022Quote: ... DNA fragments were inserted into plasmid vectors using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, Massachusetts, USA). The primers used in this study are listed in the Supplemental Table S9 ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of total RNA was used for cDNA synthesis using the LunaScript RT SuperMix Kit (New England Biolabs) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: The following M35 derivative constructs were derived from pcDNA3.1 TOPO M35-V5/His using the Q5 site-directed mutagenesis kit (#E0554, New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The inserts and backbone were assembled using the NEB HiFi DNA assembly kit (New England Biolabs, Ipswich, Massachusetts, USA), resulting in the final plasmids ...
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bioRxiv - Neuroscience 2023Quote: ... RNA-seq libraries were prepared using NEBNext® single cell/low input RNA library preparation Kit (New England Biolabs). Library preparation was initiated with 10ng of total RNA ...
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bioRxiv - Physiology 2022Quote: ... cDNA was prepared following the NEBNext® Ultra™ II Directional RNA Library Prep Kit protocol (New England Biolabs). The libraries were sequenced using paired-end 150 bp sequencing on a HiSeq 4000 System (Illumina) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Quantification of viral genomes was performed using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with a primer/probeset binding in the orf1ab region (forward ...
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bioRxiv - Molecular Biology 2022Quote: ... and in vitro transcription was performed using the HiScribe T7 ARCA mRNA Kit (New England Biolabs, Ipswich, MA, USA) to generate IVT mRNA according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... ChIP-Seq libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) and sequenced on a NovaSeq 6000 ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA-seq libraries were prepared with NEBNext Ultra Directional RNA library preparation kit for Illumina (New England Biolabs #E7420S) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ... in high output runs using a NEBNext Ultra Directional RNA Library Prep kit (New England Biolabs, Ipswich, MA, USA) including rRNA depletion ...
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bioRxiv - Cell Biology 2022Quote: mRNA was enriched from 100ng DNase treated total RNA using the NEBNext rRNA depletion Kit (human, mouse, rat, NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant plasmids encoding a catalytically inactive 3Dpol (3Dneg) were generated using the Q5 Site-Directed Mutagenesis Kit (NEB, E0554S). Nucleotides at position 6891 and 6892 of the CVB3/28 genome were mutated from AT to GC ...
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bioRxiv - Microbiology 2022Quote: ... The RNA sequencing library was prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using the manufacturer’s instructions (New England Biolabs). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by T7 in vitro transcription using the HiScribe T7 In Vitro Transcription Kit (New England Biolabs, Ipswich, MA) and purification with Trizol (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... and ChIP libraries were amplified and barcoded using the NEBNext Ultra II DNA library preparation kit (New England Biolabs) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... FLAG-tagged ORF3c mutant Q5Y was prepared by site-directed mutagenesis using a commercial kit (New England Biolabs, E0554S).
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, #E7103) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Deglycosylation of total brain homogenates was performed according to the PNGase F kit instruction manual (#P0704S, New England Biolabs). 40µg of protein were digested with 1,000 U of PNGase F enzyme.
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bioRxiv - Biophysics 2024Quote: ... The cDNAs were transcribed in vitro to cRNAs using the HiScribe® T7 Arca mRNA Kit (New England BioLabs). Single mutations in Cx26 (W3A ...
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bioRxiv - Systems Biology 2024Quote: ... 5µl of RNA (roughly corresponding to 1µg) was reverse transcribed to cDNA using the LunaScript RT SuperMix kit - dye based qPCR detection (NEB) following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... In vitro transcription (IVT) eactions were performed with HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs). Template DNA was degraded with TURBO DNase (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... and libraries for Illumina sequencing were prepared using the NEBNext Ultra II RNA kit (NEB, Ornat, Rehovot, Israel, #E7770L). Quantification of the library was performed using dsDNA HS assay kit and Qubit (Molecular Probes ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was synthesized from 1 µg total RNA using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB) with d(T)23VN primer.
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bioRxiv - Plant Biology 2024Quote: ... In-frame deletions and substitutions were generated using a Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Hitchen UK) using the primers listed in Table S1 ...
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bioRxiv - Plant Biology 2024Quote: ... The Illumina cDNA library was prepared using a NEBNext Ultra II RNA Library Prep Kit (NEB, Ipswich, MA, USA) according to the standard experimental protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Adapter ligation and library preparation were carried out using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB), and adapter ligation was checked using qPCR ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... Library preparation was carried using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7103). Sequencing was performed on an Illumina HiSeq4A to produce paired-end reds of 125 bp ...
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bioRxiv - Microbiology 2024Quote: ... Metagenomic sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep kit (New England Biolabs, MA, USA) with a targeted insert size of 350-400bp and Dual Index multiplex oligos ...
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bioRxiv - Neuroscience 2024Quote: ... Illumina library was made from polyA RNA with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s manual74 ...
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bioRxiv - Zoology 2024Quote: ... Total RNA was then extracted using a Monarch® Total RNA Miniprep Kit (New England Biolabs, Whitby, ON, Canada) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Purified RNA was depleted of ribosomal RNA (rRNA) using a NEBNext® rRNA Depletion Kit (Bacteria) (NEB, #E7850L/X) and library preparation was done using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Plant Biology 2024Quote: ... Site-directed mutagenesis transgenic plants were generated by using the Q5 ® Site-Directed Mutagenesis Kit (New England Biolabs) in the pDONR221 vector and recombined with the above-mentioned promotor constructs into the pFR7m24GW vector using the Gateway Technology ...
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bioRxiv - Synthetic Biology 2023Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra RNA library Prep Kit for Illumina per manufacturer’s instructions (New England Biolabs). mRNAs were enriched with Oligo(dT ...
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bioRxiv - Molecular Biology 2023Quote: ... pLenti-PGK-ATP6V1H(Δ176-191)-FLAG-Puro was produced using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). M4P-EGFP-LC3B and pOGP were kind gifts from F ...
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bioRxiv - Physiology 2024Quote: ... Libraries were constructed from polyA-selected RNA using a NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs) and sequenced on Illumina HiSeq2500 instrument ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The RNA sequencing library was prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs), validated on TapeStation (Agilent Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were then either purified directly using Monarch PCR and DNA Cleanup Kit (New England Biolabs, Hitchin, UK), or if more than one band was present extracted from 1% TAE gel using the QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... an equimolar pool of all amplicons was prepared and purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs). After Qubit quantification using Qubit® dsDNA HS Assay Kit and Qubit 2.0 fluorometer (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by the GFP sequence were generated by using the HiScribe T7 ARCA mRNA Kit (New England Biolabs, E2060). 1 µg mRNA was first denatured at 65°C for 3 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Mutations of PICK1 (KD-AA and 5K-E) were made with Q5® Site-Directed Mutagenesis Kit (NEB #E0554S). The constructs were purified by NucleoBond® Xtra Midi EF (MACHEREY-NAGEL) ...
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bioRxiv - Immunology 2024Quote: ... mRNA was purified from 4 µg of total RNA with a magnetic mRNA isolation kit (New England Biolabs, S1550S). cDNA was synthesized from mRNA samples with M-MLV (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ChIP-seq libraries were constructed using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs) using ChIP products from “OSC ChIP” and “Ovary ChIP” ...