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Citations for New England Biolabs :
5151 - 5200 of 9425 citations for Mouse Plectin PLEC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Micro-C libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645) following77 manufacturer instructions and sequenced on the Illumina NovaSeq 6000 platform.
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bioRxiv - Molecular Biology 2023Quote: ... The circularization of the plasmids was then achieved using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB, E5520). The primers utilized for site-specific mutagenesis are presented in Table S2 ...
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bioRxiv - Genomics 2023Quote: ... RNA expression levels were quantified using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs #E3005L) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Sequencing library preparation was made using the NEBNext® UltraTM II DNA Library Prep Kit for Illumina (NEB) following the procedure described in “Library Prep for CUT&RUN with NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (E7645 ...
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bioRxiv - Microbiology 2023Quote: ... RNA sequencing libraries were generated using the NEBNext Ultra II RNA Library Prep Kit (New England BioLabs E7760) and sequenced using a 150bp paired-end protocol on an Illumina Novaseq 6000 instrument ...
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bioRxiv - Microbiology 2023Quote: PCR was carried out using NEB Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs, E3006) or Taqman Fast Universal PCR master mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... and used to construct Illumina sequencing libraries with NEBNext Ultra Library DNA Prep Kit for Illumina (NEB, E7370). BrdU-labeled nascent DNA library fragments were then enriched by immunoprecipitation with anti-BrdU antibody (BD ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were constructed by NEBNext® Ultra RNA Library Prep Kit for Illumina (NEB, Cat# E7530L), and were sequenced by Illumina NovaSeq 6000 sequencing machine with 150-bp paired-end reads by Beijing Novel Bioinformatics Co. ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription was carried out using ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs #E6560S) with random hexamer mix ...
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bioRxiv - Cell Biology 2023Quote: ... Ribosomal RNA was depleted from 0.8 μg total RNA using NEBNext rRNA Depletion Kit (New England Biolabs, E6350L) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA libraries were prepared using the ‘NEBNext Ultra Directional RNA Library Prep Kit for Illumina’ (New England Biolabs). These samples were sequenced on the high-throughput sequencing platform (NovaSeq6000 ...
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bioRxiv - Genetics 2023Quote: ... The ints-4 sgRNA was inserted into plasmid pDD162 via the Q5 Site-Directed Mutagenesis Kit (NEB, E0554S). The sgRNA plasmid and repair template were Sanger sequenced to confirm correct construct assembly followed by microinjection into EG9882 strain with integrated Cas9 activity (10 ng µl-1 repair template ...
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bioRxiv - Neuroscience 2023Quote: RNA-seq libraries were prepared with the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs), using 1 μg total RNA per experiment.
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bioRxiv - Neuroscience 2023Quote: ... and then transcribed using pooled in vitro transcription (HiScribe T7 High Yield RNA Synthesis kit, New England BioLabs) and cleaned up with the NEB Monarch RNA Cleanup kit ...
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bioRxiv - Genomics 2023Quote: ... The NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module kit (New England BioLabs Inc. MA, USA) was used following manufacturers protocols for cDNA synthesis and amplification from 300 ng of RNA ...
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bioRxiv - Molecular Biology 2023Quote: ... the positive control and each viral mutant plasmid was constructed using the Q5 site-directed mutagenesis kit (NEB) according to manufacturer protocol and verified via whole plasmid sequencing ...
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bioRxiv - Biochemistry 2024Quote: The ATP binding mutants (APLFD11A, APLFR37A, APLFK95A) were generated using Q5® Site- Directed Mutagenesis Kit (NEB #E0554) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... RNA-seq libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770) following the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: Ifnb1 mRNA 3’ UTR (NM_002176.4) was synthesized using the HiScribe T7 High Yield RNA synthesis kit (NEB, E2040S) through in vitro transcription ...
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA was extracted from flash-frozen samples with the Monarch® Total RNA Miniprep Kit (New England Biolabs) including the DNase treatment step ...
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bioRxiv - Plant Biology 2024Quote: ... RNA-Seq libraries were prepared by NebNext Ultra II RNA library preparation kit (New England Biolabs, Massachusetts, USA) and sequenced using Illumina’s state-of-the-art NovaSeq 6000 V1.5 platform (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA synthesis and RT-qPCR was carried out using Luna® Universal One-Step RT-qPCR Kit (NEB) according to the manufacturer’s instructions with 100 ng of mRNA ...
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bioRxiv - Microbiology 2023Quote: ... We generated sequencing libraries using the TruSeq DNA PCR-Free Sample Preparation Kit (New England Biolabs, Massachusetts, USA), which were sequenced using the Illumina MiSeq PE300 platform (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: Pre-crRNA arrays (Supplementary Table 9) were synthesized using the HiScribe T7 High Yield RNA Synthesis Kit (NEB). T7 transcription was performed for 16 h and then RNA was purified using the Monarch RNA Cleanup Kit (NEB) ...
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bioRxiv - Biophysics 2023Quote: ... assembled with GST tag from pGEX6P plasmid and inserted into pACEBac1 vector via HIFI DNA assembly kit (NEB). The full length KIF5B in pACEBac1 was replaced with a C-terminal TEV-Twin-Strep-tag via HIFI DNA assembly kit (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were constructed using the NEBNext Ultra RNA Library Preparation Kit for Illumina (NEB, USA, Catalog #: E7530L) in accordance with the manufacturer’s guidelines ...
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bioRxiv - Plant Biology 2024Quote: ... before library preparation using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) and sequencing on the NovaSeq6000 (Illumina).
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bioRxiv - Cell Biology 2024Quote: ... In vitro transcription was carried out with the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs), followed by the template DNA digestion with DNase I (Takara Bio) ...
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bioRxiv - Cell Biology 2024Quote: ... Library fragment size and concentrations were quality verified by qPCR using the NEBNext Library Quant Kit (NEB, E7630) and loading 1:1 ...
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bioRxiv - Immunology 2024Quote: ... cDNA libraries were prepared using NEBNext® Ultra DNA Library Prep for Illumina® kit (NEB, catalog: E7370) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... The hsp-4 dsRNA was synthesized in vitro with a HiScribe™ T7 RNA Synthesis Kit (NEB, E2050). Subsequently ...
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bioRxiv - Synthetic Biology 2024Quote: ... and amplified PCR fragments were purified by column purification using the Monarch PCR Cleanup Kit (New England Biolabs) following the manufacturer’s protocols.
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bioRxiv - Cell Biology 2024Quote: ... Reverse transcription (RT) of RNA was performed using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs), in accordance with the manufacturer’s manual ...
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bioRxiv - Microbiology 2024Quote: ... The resulting RNA was purified and concentrated with a Monarch RNA Clean-up Kit (NEB, Ipswich, MA, USA), quantified ...
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bioRxiv - Immunology 2024Quote: ... and was subjected to in vitro transcription (IVT) using HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S). After ExoSAP-IT (Affymetrix ...
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bioRxiv - Microbiology 2024Quote: ... rRNA depletion was carried out from 100ng of total RNA with NEBNext bacterial rRNA depletion kit (NEB#e7850). NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs ...
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bioRxiv - Genomics 2024Quote: ... 200 ng of sheared DNA was processed using the NEBNext Enzymatic Methyl-seq Kit (E7120; NEB, Ipswich, MA) following the manufacturer’s instructions for large insert libraries ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Duplexed oligonucleotides were ligated into dephosphorylated and BsmBI-digested pLB-Cas9 using the Quick Ligase kit (NEB M2200S) to generate transfer plasmid with RIPK1 guide sequence ...
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bioRxiv - Immunology 2024Quote: ... and poly-A enriched before library preparation using NEBNext Ultra II Directional RNA Library Prep Kit (NEB, USA). For each library ...
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bioRxiv - Microbiology 2024Quote: ... RNA libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs) and sequenced using a NextSeq 550 75 Cycle High Output kit for single-end sequencing.
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bioRxiv - Genetics 2024Quote: ... RNA was converted to cDNA using a ProtoScript® II First Strand cDNA Synthesis Kit (NEB Cat # E6560L). RT-qPCR was performed using a QuantStudio 3 96-well 0.2 ml block instrument and PowerUp SYBR Green Master Mix (ThermoFisher Cat # A25741) ...
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bioRxiv - Developmental Biology 2024Quote: ... a pair of single guide RNAs (sgRNAs) were synthesized using the EnGen sgRNA Synthesis Kit (E3322V) from NEB; a total of 1μg of the sgRNA pair along with 20 pmol recombinant Cas9 (EnGen S ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). These libraries were subsequently sequenced for paired-end reads of 2×100 bp on an MGI system at a sequencing depth of 50X.
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bioRxiv - Molecular Biology 2024Quote: ... followed by cDNA library generation using NEBNext Ultr II Directional RNA Library Prep Kit for Illumina (NEB, E7760) according to the manufacturer’s protocol and deep sequencing on Illumina HiSeq 2500 platform.
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bioRxiv - Genomics 2024Quote: ... Psoralen pull-down DNA was further purified using a Monarch PCR & DNA clean-up kit (New England Biolabs), eluted in 15 μl of EB buffer (10 mM Tris ...
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bioRxiv - Cell Biology 2024Quote: ... Loss-of-function mutations were generated using site directed mutagenesis using Q5 Site-directed mutagenesis kit (E0552S, NEB) with the HA::SQSTM1/p62 plasmid as template (Addgene#280227) ...
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bioRxiv - Developmental Biology 2024Quote: ... Total RNA was extracted from each pool using the Monarch® Total RNA Miniprep Kit (New England Biolabs) including on-column DNase treatment ...
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bioRxiv - Molecular Biology 2024Quote: ... and libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB; E7770L). Pooled libraries were sequenced on the NovaSeq 6000 instrument using paired-end 51 cycle reads.
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bioRxiv - Cancer Biology 2024Quote: ... The library was constructed using the NEB Next®Ultra™RNA Library Preparation Kit (New England Biolabs). The library quality was assessed using the Agilent Bioanalyzer 2100 system and then sequenced using the Illumina Novaseq platform ...
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bioRxiv - Cell Biology 2024Quote: ... and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1, NEB, E7335L). Conditions for the PCR were as follows ...