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Citations for New England Biolabs :
5301 - 5350 of 9425 citations for Mouse Plectin PLEC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... were modified based on the pcDNA3.1(+)-C-I30-FLG-TEV plasmid via the Q5 Site-Directed Mutagenesis Kit (cat#: E0554S, NEB). pCMV-FLAG-ABCE1 was a gift from Dr ...
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bioRxiv - Developmental Biology 2019Quote: ... This was used as input for the NEBNext Multiplex Small RNA Library Prep Set for Illumina kit (NEB #E7300) according to the manufacturer’s recommendations with the following modifications ...
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bioRxiv - Developmental Biology 2020Quote: ... into p328-attB-ubi:EGFP (gifted from the Piotrowski Lab at Stowers Institute) (Mosimann et al., 2011) via NEBuilder HiFi assembly kit (NEB) using PCR products from p328 plasmid amplified with pDest-gibson_FW/RV primers (Table S1 ...
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bioRxiv - Cancer Biology 2020Quote: ... Point mutations were introduced by Q5 site directed mutagenesis kit according to manufacture protocol (NEB, Ispwich, MA 01938, USA). All constructs were confirmed by DNA sequencing ...
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bioRxiv - Genetics 2019Quote: ... Mutant transgenes were generated by site-directed mutagenesis of SPD771 using the Q5 Site-Directed Mutagenesis kit (NEB E0554S) and primers cdr-1p_delGATA1F (CCCTACTTTCccgctgCATTATGTCATCGGG ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and RT-qPCR was performed using the Luna Universal Onestep RT-qPCR kit (New England Biolabs, Ipswich, Massachusetts, USA) on a Bio-Rad CFX Connect Real-Time PCR Detection System (Bio-Rad Laboratories Inc. ...
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bioRxiv - Physiology 2020Quote: ... cDNA was then synthesized from 1μg RNA template through reverse transcription using Protoscript II First Strand cDNA Synthesis Kit (New England Biolabs), as recommended by manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... The sequencing library was constructed according to NEBNext Ultra II DNA library Prep kit for Illumina (NEB, cat#7645L), NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Genetics 2019Quote: RNA libraries were prepared using the NEBNext Ultra Directional RNA Library preparation kit (New England Biolabs, Ipswich, MA, USA) and sequenced on the Illumina NextSeq 500 ...
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bioRxiv - Biochemistry 2020Quote: ... and RNAse free water and lastly 1.5 μL of T7 RNA polymerase (HiScribe T7 In Vitro Transcription Kit from New England Biolabs or AmpliScribe T7 High Yield Transcription Kit from Epicenter) ...
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bioRxiv - Microbiology 2020Quote: ... an equimolar pool of all amplicons was prepared and purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs). After DNA quantification using Qubit dsDNA HS Assay Kit and Qubit 2.0 fluorometer (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2019Quote: ... The template was then used for in-vitro transcription using the HiScribe T7 High Yield RNA synthesis kit (NEB). The in-vitro transcription reaction was carried out at 37°C for 16 hrs ...
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bioRxiv - Molecular Biology 2019Quote: ... Pooled PCR products (1 μg) were digested with the NEBNext dsDNA fragmentase kit (New England BioLabs, Inc., Ipswich, MA). Fragmented DNA was end-repaired with the NEBNext End Repair Module (New England BioLabs ...
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bioRxiv - Biochemistry 2019Quote: ... PCR amplified ORF was cloned into a pGEX4T1 vector using NEB HF cloning kit (New England Biolabs, Inc. USA). E69A-TRAF6 was cloned using PCR based site-directed mutagenesis ...
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bioRxiv - Genetics 2019Quote: ... the transcriptome library was constructed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA). Furthermore ...
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bioRxiv - Cancer Biology 2019Quote: A deletion between amino acid 336 and the beginning of GFP was generated from the MCSV:GPR68-GFP plasmid using the Q5 mutagenesis kit (NEB).
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bioRxiv - Microbiology 2019Quote: ... Antisense strand DNA was digested in the samples using the Uracil-Specific Excision Reagent Enzyme Kit (New England Biolabs), followed by a PCR to amplify the cDNA ...
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bioRxiv - Microbiology 2019Quote: ... Shotgun metagenomic libraries were prepared using New England BioLabs Ultra FS II library kits (New England BioLabs, Ipswich, MA), ligated to iTru adapter stubs (18) ...
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bioRxiv - Cancer Biology 2020Quote: ... SALL4-targeting sgRNA candidates were transcribed with HiScribe™ Quick T7 High Yield RNA Synthesis Kit (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: RNA sequencing libraries were prepared for independent replicates of mRNA samples by using NEBNext Ultra RNA Library Prep Kit for Illumina according to manufacturer’s instructions (New England Biolabs). Briefly ...
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bioRxiv - Synthetic Biology 2019Quote: ... A Gibson assembly was carried out to link all DNA fragments and assemble the final integrative pHO_pCMV_CggR_ble plasmid using the NEB Gibson assembly kit (New England Biolabs, MA, US). 5 µl of reaction mix was transformed into chemical competent E.coli DH5alpha cells ...
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bioRxiv - Physiology 2021Quote: ... The biotinylated peptides were placed on streptavidin-coated plates and three rounds of solid-phase panning were conducted using 109 random 12-mer peptides fused to a minor coat protein of M13 (pIII) following the manufacture’s protocol (Ph.D-12 Phage Display Peptide Library Kit, NEB, E8110S). Sequenced data were subsequently analyzed as follows ...
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bioRxiv - Genomics 2021Quote: ... cDNA libraries were prepared using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB#E7760S) for 50ng of total RNA and using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB #E7420 ...
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bioRxiv - Genomics 2021Quote: ... for 50ng of total RNA and using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB #E7420) for 100ng of WT1 ...
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bioRxiv - Genomics 2019Quote: ... Sonicated DNA was used to construct libraries with a NEBNext Ultra DNA Library Prep Kit (NEB, Ipswich, MA, USA), and sequenced on an Illumina MiSeq instrument.
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bioRxiv - Genomics 2020Quote: ... Sequencing libraries were constructed using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA), according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... indexed ChIP-seq libraries were constructed using the NEBNext Ultra II Library Prep Kit for Illumina (New England Biolabs) using the application note ‘Low input ChIP-seq’ ...
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bioRxiv - Genomics 2021Quote: ... and were excised between 300 bp - 900 bp and cleaned using the Monarch DNA Gel Extraction Kit (NEB, T1020). The libraries were quantified and qualified using an Agilent 2100 Bioanalyzer with a High Sensitivity DNA analysis kit (Agilent ...
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bioRxiv - Genomics 2021Quote: ... DNA library preparation was performed using NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (NEB, #E7370) with 10 ng input ...
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bioRxiv - Genomics 2021Quote: ... DNA library preparation was performed using NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (NEB, E7370) with 10 ng input ...
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bioRxiv - Microbiology 2020Quote: ... 16S amplicon from all samples was prepared for sequencing using the NEBNext Illumina library preparation kit (New England Biolabs), and sequenced with 400 forward cycles of v2 Miseq chemistry.
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bioRxiv - Developmental Biology 2020Quote: ... and 6 cDNA libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) with 9 amplification cycles ...
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bioRxiv - Systems Biology 2020Quote: ... we prepared sequencing libraries using the NEBNext single-cell/low-input RNA sequencing library preparation kit for Illumina (NEB) then performed paired-end sequencing of these libraries (38 cycles read 1 + 37 cycles read 2 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We performed a 10-cycle PCR using a Phusion PCR Kit according to the manufacturer’s protocol (New England Biolabs) with multiplexed primers and adjusted PCR products to 10 μM for sequencing on either an Illumina HiSeq2500 (125 bp paired-end ...
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bioRxiv - Cell Biology 2021Quote: ... DNA was once again purified with a Qiagen MinElute kit and dA-tailed with 11.25U Klenow fragment (exo-) enzyme (NEB). DNA fragments were then selected for size (200-300bp ...
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bioRxiv - Genomics 2021Quote: ... cDNAs were generated using the ProtoScript II First Strand cDNA Synthesis Kit and Oligo(dT) primers (New England Biolabs). RT-qPCR were performed on the Light Cycler 480II (Roche ...
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bioRxiv - Genomics 2021Quote: ... After determining that some whole fly libraries made using NEBNext Ultra-II DNA library prep kit for Illumina (NEB) were as complete and has better yields than the then discontinued Nextera kit ...
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bioRxiv - Immunology 2021Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (New England Biolabs, USA) following manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... clr4 gene specific sgRNA was cloned into Cas9 containing pLSB-KAN plasmid by Golden Gate Assembly kit (E1601S, NEB). The resulting plasmid clr4-pLSB-KAN and clr4 HR template obtained by annealing primer pair WW748-clr4-HR-F/WW749-clr4-HR-R (Table S3 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The 36N plasmid DNA library was generated using a Q5 site directed mutagenesis kit (New England Biolabs, Ipswitch, MA). For amplification ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were constructed with the NEBNext® UltraTM II DNA Library Prep kit (New England Biolabs, MA, USA), and shotgun sequenced by Illumina-MiSeq at the Pittsburgh Bacteriophage Institute ...
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bioRxiv - Biochemistry 2021Quote: ... while the RBD constructs were introduced in frame to the mu phosphatase signal sequence and incorporated within pcDNA3.1 via Gibson assembly (NEBuilder HiFi DNA Assembly Cloning Kit, New England Biolabs).
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bioRxiv - Microbiology 2021Quote: ... each 180 μl sample/Saccharibacteria co-culture was treated with Endo Hf Kit P0703L (New England Biolabs, Evry, France): 3 μl of each reagent was added to the sample and incubated for one hour at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmid pES101 (encoding GPx7) was constructed by assembling three DNA fragments using the Gibson Assembly Kit (New England Biolabs). The DNA fragments used were a fragment amplified from pcDNA3.1+ (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... RNA libraries were then generated by NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB). A custom-designed probe panel (Roche) ...
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bioRxiv - Cancer Biology 2020Quote: ... and then cloned into the lentiviral expression vectors coexpressing a puromycin resistance gene or GFP (pLX_TRC307 and pLX_TRC312 from GPP) using a Gibson Assembly Cloning Kit (New England Biolabs E5510S). dTAG expression vector and dTAGV-1 compound were kindly provided by Dr ...
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bioRxiv - Cell Biology 2021Quote: ... The pDD162 plasmid was used to deliver Cas9 and sgRNAs modified using the Q5 Site-Directed Mutagenesis Kit (NEB) for sequence specific CRISPR editing of each gene ...
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bioRxiv - Cell Biology 2021Quote: CFTR-Δ23 and CFTR-W1282X were created from the synthetic CFTR high codon adaption index (HCAI) construct subcloned in the pcDNA3.1/Neo(+) vector (73) using the Q5 Site-Directed Mutagenesis Kit (NEB) with primers flanking each exon (Table S1) ...
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bioRxiv - Microbiology 2020Quote: ... The cDNA was prepared by using the First Strand cDNA Synthesis Kit following the manufacturer’s instructions (New England BioLabs). The same PCR program was applied to the total RNA sample after DNase treatment as negative controls.
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bioRxiv - Microbiology 2020Quote: ... and OC43) viral RNA genomes were synthesized using the LunaScript RT SuperMix Kit (New England Biolabs, Ipswich, MA, USA). The reaction mixture had a volume of 20◻μL and comprised of 4◻μL of 5X LunaScript RT supermix ...