Labshake search
Citations for New England Biolabs :
5201 - 5250 of 9425 citations for Mouse Plectin PLEC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The library preparation was elaborated using the NEBNext® SingleCell/Low Input cDNA Synthesis kit (New England Biolabs). The prepared libraries were then sequenced ...
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bioRxiv - Biochemistry 2024Quote: ... Then In-column DNase I Treatment and RNA cleanup was done using the Monarch RNA Cleanup Kit (NEB). Then total RNA was sent to QB3-Berkeley Genomics facility at the University of California ...
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bioRxiv - Immunology 2024Quote: ... DNA libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (cat. E7645S, New England Biolabs) and purified with AMPure SPRI beads (cat ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were constructed using the NEBNext Ultra RNA Library Preparation Kit for Illumina (NEB, USA, Catalog #: E7530L) in accordance with the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2024Quote: Substrate ssRNA was transcribed using HiScribe T7 High Yield RNA Synthesis Kit (New England BioLabs, Ipswich, MA, SUA) and was [32P]-labelled at the 5’-end using T4 polynucleotide kinase (New England BioLabs ...
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bioRxiv - Molecular Biology 2024Quote: ... and libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB; E7770L). Pooled libraries were sequenced on the NovaSeq 6000 instrument using paired-end 51 cycle reads.
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bioRxiv - Neuroscience 2024Quote: Genomic DNA from SH-SY5Y cells and iPSCs was extracted with the Monarch Genomic DNA Purification Kit (NEB). PCR was performed with Q5 Hot Start High-Fidelity Master Mix (NEB ...
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bioRxiv - Biochemistry 2024Quote: Illumina libraries were prepared using NEBNext Ultra DNA II Library Preparation Kit (New England Biolabs, Ipswich, MA, USA). 500 ng of DNA was fragmented using a Covaris E220 ...
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bioRxiv - Biochemistry 2024Quote: ... PCR products were analyzed by agarose gel electrophoresis and purified with Monarch PCR and DNA Cleanup Kit (NEB). On a passivated glass coverslip ...
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bioRxiv - Molecular Biology 2024Quote: Genomic DNA was harvested from sgRNA-transduced cells using either the Monarch genomic DNA purification kit (NEB, #T3010S) or QuickExtract DNA extraction solution (Lucigen ...
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bioRxiv - Immunology 2024Quote: ... cDNA libraries were prepared using NEBNext® Ultra DNA Library Prep for Illumina® kit (NEB, catalog: E7370) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Library preparation was done with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7770L), following the recommended kit protocol ...
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bioRxiv - Cell Biology 2024Quote: ... In vitro transcription was carried out with the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs), followed by the template DNA digestion with DNase I (Takara Bio) ...
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bioRxiv - Cell Biology 2024Quote: ... Library fragment size and concentrations were quality verified by qPCR using the NEBNext Library Quant Kit (NEB, E7630) and loading 1:1 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We also performed a fifth extraction with spleen tissue using Monarch® HMW DNA Kit (New England BioLabs) for Ultra-Long library preparation.
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bioRxiv - Immunology 2024Quote: ... and was subjected to in vitro transcription (IVT) using HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S). After ExoSAP-IT (Affymetrix ...
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bioRxiv - Microbiology 2024Quote: ... The resulting RNA was purified and concentrated with a Monarch RNA Clean-up Kit (NEB, Ipswich, MA, USA), quantified ...
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bioRxiv - Microbiology 2024Quote: ... rRNA depletion was carried out from 100ng of total RNA with NEBNext bacterial rRNA depletion kit (NEB#e7850). NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs ...
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bioRxiv - Cell Biology 2024Quote: ... Reverse transcription (RT) of RNA was performed using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs), in accordance with the manufacturer’s manual ...
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bioRxiv - Plant Biology 2024Quote: ... before library preparation using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) and sequencing on the NovaSeq6000 (Illumina).
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bioRxiv - Developmental Biology 2024Quote: ... The hsp-4 dsRNA was synthesized in vitro with a HiScribe™ T7 RNA Synthesis Kit (NEB, E2050). Subsequently ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We prepared double-stranded libraries with the NEBNext Ultra II DNA library preparation kit (New England Biolabs: NEB) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We prepared double-stranded libraries with the NEBNext Ultra II DNA library preparation kit (New England Biolabs: NEB) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... the second strand synthesis was carried out utilizing the NEBNext Second Strand Synthesis Kit (New England Biolabs, E6111S).
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bioRxiv - Genomics 2024Quote: ... 200 ng of sheared DNA was processed using the NEBNext Enzymatic Methyl-seq Kit (E7120; NEB, Ipswich, MA) following the manufacturer’s instructions for large insert libraries ...
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bioRxiv - Synthetic Biology 2024Quote: ... and amplified PCR fragments were purified by column purification using the Monarch PCR Cleanup Kit (New England Biolabs) following the manufacturer’s protocols.
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bioRxiv - Microbiology 2024Quote: ... 500 ng of total RNA underwent ribosomal depletion with the NEBNext rRNA Depletion Kit (Bacteria) (NEB catalog # E7850) and library preparation with the TruSeq Stranded Total RNA LT Kit (Illumina catalog # RS-122-1202 ...
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bioRxiv - Synthetic Biology 2021Quote: ... total DNA from one clone was isolated using the Monarch Kit for HMW DNA Extraction from Bacteria (NEB#T3060) and confirmed by Nanopore sequencing.
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative RT-PCR was performed using Luna Universal One-Step RT-qPCR kit (New England Biolabs, Ipswich, MA, USA) according to manufacturer’s protocol using Agilent Mx3000P instrument ...
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bioRxiv - Cancer Biology 2021Quote: ... Renilla luciferase gene and Firefly luciferase gene were in-vitro transcribed using HiScribe T7 RNA Synthesis Kit (NEB Biolabs). After purified with Monarch RNA Cleanup Kit (NEB Biolabs) ...
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bioRxiv - Cancer Biology 2021Quote: ... Renilla luciferase gene and Firefly luciferase gene were in-vitro transcribed using HiScribe T7 RNA Synthesis Kit (NEB Biolabs). After purified with Monarch RNA Cleanup Kit (NEB Biolabs) ...
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bioRxiv - Cancer Biology 2021Quote: ... before they were used for library construction using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). Adaptors were removed from the 3’ end of reads using cutadapt (Martin ...
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bioRxiv - Microbiology 2020Quote: ... RNA was isolated using Invitrogen PureLink RNA Mini Kit following manufacturer’s instructions and treated with DNase (New England Biolabs). Biological triplicate samples were submitted to DNA Technologies and Expression Analysis Core at UC Davis Genome Center for the generation of 3’Tag RNA-Seq libraries ...
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bioRxiv - Microbiology 2020Quote: Site-directed mutagenesis was performed on pBPVP1456 with a Q5 site-directed mutagenesis kit (New England Biolabs, Ipswich, MA) and primers listed in Table 3 ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA-Seq libraries (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, New England BioLabs, Catalog No. E7760) were produced from 16 samples according to the manufacturer’s protocol using 200 ng total RNA ...
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bioRxiv - Cell Biology 2020Quote: RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... addition of a N-terminal Hemagglutinin (HA) epitope was done using Q5 Site-Directed Mutagenesis Kit (New England Biolabs) following manufacturer recommendations.
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bioRxiv - Immunology 2021Quote: ... Devil CIITA was then cloned into SfiI-digested pSBbi-BH using NEBuilder® HiFi DNA Assembly Cloning Kit (NEB). The assembled plasmid pCO2 was transformed into NEB® 5-alpha competent Escherichia coli (High Efficiency ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 RNA levels were quantified using the Luna Universal Probe Onestep RT-qPCR kit (New England Biolabs) and US CDC real-time RT-PCR primer/probe sets for 2019-nCoV_N1 ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB). The sequencing libraries were validated on the Agilent TapeStation (Agilent Technologies) ...
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bioRxiv - Developmental Biology 2021Quote: ... and PCR addition of indices (7-11 cycles) was done using NEBNext Ultra II DNA library prep kit (NEB). Biotinylated capture probes 70 nt in length were designed against every DpnII restriction fragment in a 2.5 Mb window centered on Runx1 (chr16:91,566,000-94,101,999) ...
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bioRxiv - Genetics 2021Quote: ... and ligated with the amplicons Uro promoter and GeneSwitch using NEBuilder HiFi DNA Assembly Kit (NEW ENGLAND BioLabs, E2621X). Transgenic lines were generated using standard methods for P-element-mediated germline transformation (BestGene Inc).
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bioRxiv - Genetics 2021Quote: ... The synthetic oligo was cloned into the plasmid backbone by using the Gibson assembly kit (NEB, Gibson Assembly®) and the ligation reaction was carried out for 1 hour at 50 °C ...
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bioRxiv - Genetics 2021Quote: ... High molecular weight gDNA extraction using a Monarch® HMW DNA Extraction Kit for Tissue (New England Biolabs, T3060L) is performed on tissues isolated from N1 or higher generation mice ...
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bioRxiv - Genomics 2020Quote: ... Sequencing libraries were prepared with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, E7645) according to protocol ...
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bioRxiv - Genomics 2020Quote: ... DNA library was prepared using NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (New England BioLabs) with 5cycles of pre-amplification and 3 to 7 cycles of PCR amplification ...
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bioRxiv - Molecular Biology 2020Quote: ... and then a cDNA library was prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB E7530L). The libraries were checked for quality using the Fragment Analyzer NGS Analysis DNF-474 kit (Advanced Analytical ...
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bioRxiv - Cell Biology 2020Quote: Resulting amplicons from the second PCR were column purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs; NEB) to remove genomic DNA and first round PCR product ...
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bioRxiv - Cell Biology 2020Quote: Resulting amplicons from the second PCR were column purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs; NEB) to remove genomic DNA and first round PCR product ...
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bioRxiv - Cancer Biology 2020Quote: ... Strand-specific libraries were created using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, Hitchin, Herts, UK) and 20ng of the rRNA depleted RNA.