Labshake search
Citations for Illumina :
4201 - 4250 of 4662 citations for Genz 644282 CAS 529488 28 6 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... Approximately 5 ug of total RNAs were used to construct cDNA libraries (NEBNext Ultra Directional RNA Library Prep Kit for Illumina, Illumina, San Diego, CA, USA). Transcriptome sequencing was performed using the Illumina Hi-Seq 2000 platform ...
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bioRxiv - Neuroscience 2020Quote: ... sequencing was performed at a targeted depth of 40 million reads per sample using the Illumina NextSeq 500 platform (Illumina Inc, San Diego, CA). mRNA reads (4 regions ...
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bioRxiv - Genomics 2021Quote: ... Eight whole genome sequencing libraries with a median insert size of 550 bp were constructed using the Illumina TruSeq DNA PCR-free sample preparation kit (Illumina, San Diego, CA, USA), according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... Extracted DNA was sent to the University of Illinois Keck Center for Fluidigm sample preparation and paired-end (2 × 250 nt) sequencing on an Illumina NovaSeq (Illumina, San Diego, CA, USA). The 16S rRNA gene fragment was amplified using the primers V3_F357_N ...
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bioRxiv - Microbiology 2021Quote: ... Libraries were then sequenced on a NovaSeq 6000 instrument with a SP flow cell (2 x 250 bp) (Illumina Inc., San Diego, CA, USA).
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bioRxiv - Microbiology 2020Quote: ... Samples were sequenced following amplification of the V4 hypervariable region of the 16S rRNA gene with modified F515/R806 primers [21] on a MiSeq platform (Illumina, San Diego, CA, USA) in 2016 (Batch 1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were then processed at The Center of Functional Genomics/University at Albany for differential expression with Poly-A selection and sequenced using an Illumina Nextseq500 (Illumina, Inc., San Diego, CA) at 75 million single-end reads per run ...
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bioRxiv - Microbiology 2020Quote: ... of the 16S rRNA gene and the internal transcribed spacer (ITS) 1 rDNA variable region on the Illumina MiSeq platform (Illumina, San Diego, CA, USA), as detailed previously (Phoma et al. ...
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bioRxiv - Microbiology 2020Quote: ... The RNA extracts were enriched for bacteria by depleting coral host rRNA using a Ribo-Zero Gold Yeast kit (Illumina, San Diego, CA, USA) following the manufacturer’s protocols.
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bioRxiv - Cancer Biology 2020Quote: Libraries for cluster generation and DNA sequencing were prepared following an adapted method from Fisher et al49 using Illumina TruSeq Stranded mRNA LT Kit (Illumina, San Diego, CA, USA). Quality and quantity of the DNA libraries was assessed on a Agilent 2200 Tapestation (D1000 screentape ...
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bioRxiv - Cancer Biology 2020Quote: ... The libraries were run on the Illumina Next Seq 500 using the High Output 75 cycles kit (2 x 36 cycles, paired end reads, single index; Illumina, San Diego, CA, USA).
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bioRxiv - Cancer Biology 2020Quote: ... converted to cDNA and ligated to sequencing adaptors containing indexes using the Illumina TruSeq stranded mRNA sample preparation kit (Cat. #RS-122-2101, Illumina, Inc., San Diego, CA). The molar concentrations of the indexed libraries were measured using the 2100 Agilent Bioanalyzer (Agilent Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA (1 μg) was then converted into cDNA libraries according to the Illumina TruSeq Stranded mRNA Sample Preparation Guide (Illumina, San Diego, CA, USA) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... with RNA integrity number higher than 7 was used for library preparation using the TruSeq Stranded mRNA Library Preparation Kit (Illumina, San Diego, CA, USA) and sequenced on a NextSEq500 (Illumina) ...
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bioRxiv - Bioengineering 2020Quote: ... RNA-Seq libraries were constructed by using Illumina HiSeq 2500 Stranded Total RNA TruSeq RNA Library Preparation Kit v2 (Illumina, San Diego, CA, USA) and sequenced on the Illumina Nova Seq 6000 platform in the SR 50bp and barcode (Illumina) ...
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bioRxiv - Genomics 2021Quote: ... For Illumina sequencing an Illumina Nextera XT library was prepared for paired-end sequencing on an Illumina NextSeq550 (Illumina Inc., San Diego, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... 2ng of DNA was prepared in 30ul of buffer and used for library construction using the Nextera DNA Flex Library Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2022Quote: The RNA samples were DNase-treated and then underwent poly-A enrichment and library construction using a TruSeq v2 Library Preparation Kit (Illumina, San Diego, CA, USA), followed by 150 bp paired-end sequencing on an Illumina HiSeq 3000 platform at the Max Planck-Genome center (Cologne ...
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bioRxiv - Neuroscience 2022Quote: ... and libraries were constructed by using 600 ng of total RNA from each sample and utilizing a TruSeqV2 kit from Illumina (Illumina, San Diego, CA) following the manufacturer’s suggested protocol ...
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bioRxiv - Genomics 2022Quote: ... Then 16S rRNA gene sequencing libraries were prepared according to Illumina’s protocol and run on an Illumina MiSeq for 300 cycles of paired-end sequencing using a MiSeq v3 600-cycle reagent kit (Illumina, San Diego, CA, USA). For S1 ...
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bioRxiv - Immunology 2022Quote: ... A total of 250ng total RNA was used for each sample as input to the TruSeq Stranded Total RNA library prep kit with unique-dual indexing (catalog #s 20020598 & 2002371; Illumina, San Diego, CA, USA). Libraries were assessed for mass using the Qubit 1X dsDNA HS Assay kit (catalog # Q33231 ...
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bioRxiv - Immunology 2022Quote: ... Paired-end (2×75) sequencing was performed to an average of 40 million reads per sample on a HiSeq 3000 (Illumina, San Diego, CA, USA).
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bioRxiv - Molecular Biology 2022Quote: ... a final dilution to 4nM was performed and 13 pM of the final denaturated library was loaded onto the Illumina MiSeq® with 10% PhiX using 2 × 300 bp v3 chemistry (Illumina Inc., CA, USA).
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bioRxiv - Microbiology 2022Quote: ... a paired-end metagenomic library was prepared from 100 ng of DNA using the DNA PCR free Library Prep Kit (Illumina, San Diego, CA, USA) and size selected at about 400 bp ...
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bioRxiv - Microbiology 2022Quote: ... sequencing libraries were prepared using Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit per manufacturer’s protocol (Illumina Inc., San Diego, CA, USA) and sequenced on an Illumina MiSeq at 2 x 150 base pair reads ...
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bioRxiv - Neuroscience 2022Quote: ... the mRNA from each brain sample was pooled for RNA-Seq library construction using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, USA). The mRNA library was subjected to paired-end transcriptome sequencing (Illumina platform) ...
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bioRxiv - Genomics 2022Quote: ... Canada prepared and sequenced four additional libraries: one paired-end (PE) library with 550 bp inserts using a Nano kit (Illumina, San Diego, CA, USA) and three mate-pair (MP ...
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bioRxiv - Genomics 2022Quote: ... Five hundred ng were used as starting material for library preparation using the TruSeq® Stranded mRNA Library Prep (Illumina, Inc, San Diego, CA) and the IDT-ILMN TruSeq UD indexes ...
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bioRxiv - Genetics 2022Quote: ... Indexed libraries prepared from small RNA were normalized and all 24 libraries were pooled and split between two lanes of a MiSeq (Illumina, Inc., San Diego, CA) for 50 base-pair single read sequencing.
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bioRxiv - Genetics 2022Quote: ... Retinal cDNA libraries were created by the Iowa Institute of Human Genetics Genomics Division using the TruSeq Stranded mRNA Library Prep Kit and the TruSeq Small RNA Library Prep Kit (Illumina, Inc., San Diego, CA). Indexed libraries prepared from mRNA were normalized and pooled into three groups (corresponding to three biological replicates ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We then prepared dual indexed-libraries using 1ng DNA extract using the Nextera XT Library Preparation Kit and the Nextera XT Index Kit (Illumina, San Diego, CA, USA), which we pooled so individual libraries would all bring in about the same number of HSV-2 genome copies (according to the qPCR results) ...
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bioRxiv - Genomics 2022Quote: ... Integrity of genomic DNA was verified by the DKFZ Genomics and Proteomics Core Facility and 100-250 ng subjected to DNA methylation analysis using Infinium Mouse Methylation BeadChip arrays (Illumina, San Diego, CA, USA).
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bioRxiv - Genomics 2022Quote: ... SW-8046-2) from libraries prepared using the Nextera XT DNA kit (Illumina, Inc, San Diego, CA, and sequenced on Illumina HiSeq4000 (2×150 bp). WGS data was obtained from six C ...
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bioRxiv - Cancer Biology 2020Quote: ... The amplification and sequencing of 16S rRNA gene V3V4 region were done commercially by Omega Bioservices (Georgia, USA) on a MiSeq platform (Illumina, Inc, San Diego, CA) using the 2×300 bp paired-end protocol ...
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bioRxiv - Microbiology 2020Quote: ... The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumina; San Diego, CA, USA), and the library preparations were then sequenced on an Illumina NovaSeq 6000 platform (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... The DNA library was prepared using the Nextera XT library preparation kit with the Nextera XT v2 index kit (Illumina, San Diego, CA, USA). The library fragment length was aimed at fragments with a median size of 575 bases and was assessed with the Genomic DNA ScreenTape assay with the 2200 Tape-Station system (Agilent Technologies ...
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bioRxiv - Neuroscience 2020Quote: DNase treated RNA was subjected to poly-A enrichment and library construction using a RNA-Seq v2 Library Preparation Kit (Illumina, San Diego, CA, USA), followed by 150 bp paired-end sequencing ...
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bioRxiv - Plant Biology 2021Quote: ... Pooled libraries were diluted to 1.8 pM for 1×86 bp single-end sequencing with 75-cycle High Output v2 Kit on the NextSeq 550 Sequencing System (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... An Illumina paired-end DNA library with an insert size of ∼300 bp was constructed using the Genomic DNA Sample Prep kit (Illumina, San Diego, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... The cDNA libraries were posteriorly sequenced on the Illumina Genome platform Analyzer IIx with a TruSeq kit with 36 cycles (Illumina, San Diego, CA, USA) for 72 bp paired-end reads.
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bioRxiv - Microbiology 2020Quote: ... One nanogram of quantified DNA was sheared enzymatically at 55°C for 5 min using the Illumina Nextera XT tagmentation enzyme (Illumina, San Diego, CA, USA). Tagmented DNA fragments were enriched by 10 cycles of PCR amplification using PCR master mix and primers with the index from Illumina ...
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bioRxiv - Microbiology 2020Quote: ... and were sequenced at the University of Washington Northwest Genomics Center with the Illumina NextSeq 500 High Output v2 Kit (Illumina Inc, San Diego, CA). The sequencing generated an average of 75 million base-pair paired-end raw read counts per library.
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bioRxiv - Microbiology 2020Quote: ... Next Generation Sequencing (NGS) was performed on an Illumina MiniSeq® using a Mid Output Kit (2×150-cycles) (Illumina, San Diego, CA, USA) by loading 0.8 pM on the flowcell ...
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bioRxiv - Genomics 2020Quote: ... The pooled library was quality controlled via sequencing at a concentration of 1.7 pM with 35% PhiX on a NextSeq 500 using a mid-output v2 kit (single-end 75 nucleotides, Illumina, San Diego, CA, USA), resulting in an average sequencing depth of 1 million reads ...
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bioRxiv - Genomics 2020Quote: Small RNA libraries were prepared starting from 5 µL DNase treated and spike-in supplemented RNA eluate using a TruSeq Small RNA Library Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol with two minor modifications(1) ...
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bioRxiv - Genomics 2020Quote: ... Both re-pooled libraries were then sequenced at a final concentration of 1.7 pM with 25% PhiX on a NextSeq 500 using a high output v2 kit (single-end, 75 nucleotides, Illumina, San Diego, CA, USA), resulting in an average sequencing depth of 9 million reads (range 817 469 – 41.7 million reads).
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bioRxiv - Neuroscience 2020Quote: ... paired-end sequencing was performed on an Illumina NextSeq 500 sequencer using a 150 bp sequencing kit (v2) (Illumina Inc., San Diego, CA, USA) to produce a minimum of 50M paired-reads per library ...
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bioRxiv - Microbiology 2021Quote: ... To prepare 500 bp paired-end libraries of all isolates we used the Nextera XT DNA Library Preparation kit (Illumina, San Diego, CA, USA). Libraries were sequenced on the Illumina MiSeq sequencing platform using v2 sequencing chemistry ...
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bioRxiv - Biochemistry 2021Quote: ... Amplicons containing the mutagenized regions were prepared from each cellular isolate and sequenced using paired end reads on a 600 cycle Illumina MiSeq cell (Illumina Inc., San Diego, CA) to a final depth of ~2 million reads per amplicon/ isolate ...
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bioRxiv - Molecular Biology 2022Quote: ... miRNA libraries were prepared from the isolated EV RNA and a water control using the TruSeq Small RNA Library Preparation Kit (RS-200-0012, Illumina, San Diego, CA, USA) using the manufacturer instructions with the modification of performing the adaptor ligation in the presence of 15% polyethylene glycol in order to reduce bias by driving the ligation reactions towards completion as determined by the Extracellular RNA Communication Consortium (46) ...