Labshake search
Citations for Illumina :
301 - 350 of 669 citations for 1 1 Dimethylsila 11 Crown 4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Enterobacter sp. SA187 mediates plant thermotolerance by chromatin modification of heat stress genesbioRxiv - Plant Biology 2020Quote: We performed mRNA libraries with 1 µg of total plant RNA using a stranded mRNA Library Prep kit (Illumina). Pooled libraries were sequenced using Illumina HiSeq 4000 platform which resulted in paired-end reads of length 151 bps ...
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bioRxiv - Genetics 2020Quote: ... were incubated in a 50 µl reaction with 0.8 µl of Tn5 transposase at 1× TD buffer from the Nextera library preparation kit (Illumina) for 5 min at 55°C ...
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bioRxiv - Genomics 2021Quote: ... Complementary DNA (cDNA) libraries were prepared from 1 μg RNA using TrueSeq RNA Sample Prep Kit (Illumina, California, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... adding Illumina sequencing adapters and dual-index barcodes (index 1(i7) and index 2(i5)) (Illumina, San Diego, CA) to the amplicon target ...
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bioRxiv - Bioengineering 2022Quote: ... and 1 ×107 cells from each gate were barcoded and the sequences for each cell were determined by Illumina next-generation sequencing15 ...
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bioRxiv - Genomics 2022Quote: Combined with four types of libraries constructed: (1) the short-read sequencing library with 350-bp insertions from Illumina HiSeq X Ten instrument platforms ...
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bioRxiv - Immunology 2020Quote: ... Libraries were sequenced on the iSeq (cases 1 - 6 and controls) or NovaSeq 6000 (case 7 and controls) (Illumina) using 150nt paired-end reads.
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bioRxiv - Cell Biology 2021Quote: ... Samples were sequenced according to the Illumina user manual with 80 cycles of read 1 (forward) using the NextSeq 500/550 High Output Kit v2.5 (75 Cycles) (Illumina) with the 20% PhiX spike in Illumina PhiX control kit (Illumina).
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bioRxiv - Microbiology 2021Quote: ... All cDNA libraries were sequenced on an Illumina NextSeq 500 instrument at the Boston University microarray and sequencing core according to Illumina and 10x Genomics guidelines with 1.4-1.8pM input and 1% PhiX control library spike-in (Illumina, USA).
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bioRxiv - Molecular Biology 2022Quote: ... The chromatin was then tagmented by resuspending beads in 29 µl Tagmentation Buffer (10 mM Tris-HCl pH 8.0, 5 mM MgCl2, 10% dimethylformamide) and adding 1 µl of transposase (Illumina). Samples were incubated at 37 °C for 10 min and the reaction was terminated by adding 150 µl RIPA buffer ...
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bioRxiv - Genetics 2022Quote: ... sequence reads were mapped to the mouse reference genome GRCm38/mm10 (iGenomes, Illumina; chromosomes 1-19, X, Y, M) using the Rsubread v1.28.1 package in R v3.4.4 ...
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bioRxiv - Genetics 2020Quote: ... for PPI (see supplementary methods).14 The 148 additionally selected SNV markers (supplementary table 1) were genotyped in 1,012 F2 mice by Illumina BeadArray genotyping (Illumina Golden Gate assay ...
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bioRxiv - Synthetic Biology 2021Quote: ... cDNA was diluted to an average of 200 pg/µl and 100 pg cDNA from each cell was tagmented by adding 1 µl TD and 0.5 µl ATM from a Nextera XT DNA Library Preparation Kit (Illumina) to 0.5 µl diluted cDNA in each well of a fresh 384-well plate ...
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bioRxiv - Immunology 2020Quote: ... RNA-Seq libraries were prepared using 1 μg of end-repaired cDNA using the TruSeq Stranded RNA Kit (Illumina), however due to the method of amplification the libraries were not stranded ...
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bioRxiv - Genomics 2021Quote: ... 30 μL of NEBNext Ultra II Ligation Master Mix, 1 μL of NEBNext Ligation Enhancer, 2.5 μL of NEBNext Adapter for Illumina) at 20 °C for 15 min. ...
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bioRxiv - Microbiology 2022Quote: ... RNA-Seq libraries were prepared using 1 ug of total RNA with TruSeq Stranded mRNA Library Prep kit (Illumina) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... All cDNA libraries were sequenced on an Illumina NextSeq 500 instrument according to Illumina and 10X Genomics guidelines with 1.4–1.8 pM input and 1% PhiX control library spike-in (Illumina, USA). Sequencing data were processed using 10X Genomics’ Cell Ranger pipeline to generate feature/barcode matrices from raw count data.
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bioRxiv - Developmental Biology 2023Quote: ... bead-bound complexes were resuspended in tagmentation mixture (25µL reaction containing 1 µL of enzyme in 1X buffer, Illumina) and incubated at 37°C for 25minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... Sequencing libraries were prepared using 1 μg of total RNA with the TruSeq Stranded mRNA Sample Prep Kit (Illumina) and the TruSeq single-index adaptor (Illumina) ...
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bioRxiv - Pathology 2023Quote: ... Barcoded libraries from each experimental sample were combined in equimolar concentrations of 1.5 pM prior to sequencing at 75bp x 1 (single-end) read metric on a NextSeq 550 (Illumina) system.
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bioRxiv - Immunology 2023Quote: ... The samples were sequenced in a paired-end run (read 1: 28bp, read 2: 91bp) on a NovaSeq6000 platform using S1 v1.5 (100 cycles) sequencing kits (Illumina). Bcl2fastq software (v2.20.0.422 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 ng DNA was used to generate the sequencing library by using Nextera XT DNA Library Preparation Kit (Illumina).
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bioRxiv - Bioengineering 2023Quote: ... 10x libraries were pooled and charged with 1% PhiX on one SP lane of the NovaSeq 6000 instrument (Illumina) using the NovaSeq 6000 SP Reagent Kit v1.5 Raw sequencing data of the modules viability study were processed with Cellranger80 ...
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bioRxiv - Molecular Biology 2023Quote: ... Pelleted nuclei were incubated in transposase reaction mix (1 X TD reaction buffer, 2.5 µl Tn5 transposase (Nextera, Illumina)) for 30 min at 37 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR product was purified in a 1 % agarose gel and sequenced using a NextSeq 550 and NovaSeq (Illumina) to obtain 79+79-nt ...
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bioRxiv - Plant Biology 2022Quote: The triplicate total RNA samples used for single-end 1×75bp RNA-Seq on a NextSeq500 sequencing platform (Illumina). After examining the data quality using FastQC (v0.11.5) ...
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bioRxiv - Genomics 2022Quote: ... 5 μl of Index primer (both provided in NEBNext Multiplex Oligos for Illumina Index Primers Sets 1 and 2), and 25 μl NEBNext Ultra II Q5 Master Mix (PCR cycling conditions ...
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bioRxiv - Plant Biology 2023Quote: ... The library was diluted to 0.5 nM with EB in 18 μL and spiked with 1% PhiX Control v3 (Illumina) before being prepared for loading using a NovaSeq XP 2 lane kit v1.5 (20043130 ...
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bioRxiv - Immunology 2022Quote: ... 5,000-50.000 cells per experiment were centrifuged 5 minutes at 8°C, and resuspended in 25 µl transposase mixture (12.5 µl Tagmentation DNA buffer, 1 µl Tn5 transposase (Illumina), 10.75 µl nuclease-free water ...
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bioRxiv - Microbiology 2023Quote: ... 1 ng of DNA was used to prepare DNA libraries using the Nextera XT DNA Library Preparation Kit (Illumina) and genomic DNA was fragmented with Illumina Nextera XT fragmentation enzyme ...
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bioRxiv - Developmental Biology 2024Quote: ... The mRNA-Seq libraries were prepared with 1 μg of total RNA using the TruSeq RNA Library Prep Kit v2 per the manufacturer’s instructions (Illumina). Three independent biological replicates were prepared for each condition and libraries were sequenced on an Illumina HiSeq 4000 instrument (single-end ...
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bioRxiv - Genomics 2024Quote: The read 1 fastq file from each spatial region was mapped on the mm10 reference genome (source: Illumina igenomes) using the STAR package (v 2.7.9a ...
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bioRxiv - Cell Biology 2024Quote: ... we prepared the mRNA-Seq libraries using 1 μg of total RNA and the TruSeq RNA Library Prep Kit v2 according to the manufacturer’s instructions (Illumina). The samples were sequenced on an Illumina HiSeq 4000 instrument (single-end ...
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bioRxiv - Genomics 2024Quote: ... We added a FORMAT (GT) and SAMPLE (0/1) column to the VCF and used hap.py (https://github.com/Illumina/hap.py) to compare the v4.2.1 benchmark VCF and BED (https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/ChineseTrio/HG005_NA24631_son/NISTv4.2.1/GRCh38/) ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR product was purified from a 1% agarose gel and sequenced using a NextSeq 550 or NovaSeq (Illumina) to obtain 79+79-nt or 150+150-nt ...
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bioRxiv - Immunology 2024Quote: ... All cDNA libraries were sequenced on an Illumina NextSeq 500 instrument according to Illumina and 10x Genomics guidelines with 1.4-1.8pM input and 1% PhiX control library spike-in (Illumina, USA).
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bioRxiv - Physiology 2024Quote: ... 10x libraries were pooled and charged with 1% PhiX on one S1 lane of the NovaSeq 6000 instrument (Illumina) using the NovaSeq 6000 S1 Reagent Kit v1.5 (100 cycles) ...
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bioRxiv - Neuroscience 2024Quote: ... 5 ul of Tn5 tagmentation mix (1X Tagment DNA buffer, Illumina; ATM mix, Illumina and cDNA library 1 ng) was prepared for cDNA fragmentation ...
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bioRxiv - Developmental Biology 2024Quote: ... cleaned up by 0.6:1 AMPure XP beads and sequenced on NovaSeq for 150-bp paired end reads (Illumina).
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bioRxiv - Cancer Biology 2024Quote: ... diluted to 650 pM in RSB buffer and mixed with 1% PhiX Control v3 DNA (Illumina FC-110-3001) for sequencing ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA libraries were created from 1 µg DNase-treated RNA with the TruSeq Stranded Total RNA Globin kit (Illumina) and sequenced on the Illumina NovaSeq6000 ...
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bioRxiv - Developmental Biology 2024Quote: ... cleaned up by 0.6:1 AMPure XP beads and sequenced on NovaSeq for 150-bp paired end reads (Illumina).
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bioRxiv - Cancer Biology 2021Quote: ... and sequenced on an Illumina HiSeq 2000 sequencer (RRID:SCR_020132, v.4, Illumina, San Diego, California, USA) in 50 bp single-end mode by Genomics and Proteomics Core facility ...
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bioRxiv - Microbiology 2023Quote: ... 4 μl of the 3’-5’-adapter-ligated RNA was mixed with barcoded RT primers (Illumina) and cDNA synthesis was performed using SuperScript™ II Reverse Transcriptase (Invitrogen ...
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bioRxiv - Genomics 2024Quote: ... samples were incubated in cleavage mix (MiSeq Nano kit v2 reagent 4) (Illumina MS-103-1003) for 6 min at 60 °C ...
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bioRxiv - Microbiology 2022Quote: ... and diluted to 4 nM for sequencing on an Illumina MiSeq (Illumina, San Diego, CA, USA). A 500 cycle MiSeq Reagent kit v2 (Illumina ...
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bioRxiv - Cell Biology 2020Quote: ... RNA-Seq libraries were generated from 1 μg of total RNA from duplicated samples per condition using the TruSeq LT RNA Library Preparation Kit v2 (Illumina) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... Libraries were sequenced by NextSeq 550 High Output with 21 bp for read 1 and 72 bp for read 2 (Illumina). Sequenced reads were aligned using STAR (v2.7.6a ...
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bioRxiv - Genetics 2021Quote: ... The scRNAseq samples were then sequenced on 1/7th of a single S4 lane on a NovaSeq 6000 platform (Illumina). For the ScISOr-Seq library ...
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bioRxiv - Microbiology 2020Quote: ... pooled with 1% denatured PhiX DNA (sequencing control) and sequenced in a MiSeq platform (2× 251 cycles paired-end run; Illumina). New PCR reactions using combinations of the primers described above were carried out to cover regions with low coverage for each sample ...