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Citations for Illumina :
451 - 500 of 669 citations for 1 1 Dimethylsila 11 Crown 4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Nuclei were then pelleted (1,000xg, 10min, 4°C) and transposed in 2.5µl Tn5 (Nextera Tn5 transposase, Illumina), 12.5µl TD reaction buffer and 10.5µl water for 40min at 37°C with rotation ...
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bioRxiv - Genomics 2020Quote: ... The library was sequenced on 4 lanes of an Illumina HiSeq 1500 (Illumina Inc.; San Diego, CA), generating 450,132,548 reads which were subsequently trimmed to remove adapters and filtered for length and quality using FASTX-Toolkit v0.0.14 (available from http://hannonlab.cshl.edu/fastx_toolkit/) ...
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bioRxiv - Genomics 2021Quote: ... Libraries were diluted to 4 nM and sequenced on a NextSeq 500 Sequencing System (Illumina, California, USA) using NextSeq 500/550 High Output Kit v2 (150 cycles ...
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bioRxiv - Genomics 2021Quote: ... The library pool was sequenced on all 4 lanes of an NovaSeq 6000 S4 flow cell (Illumina) for 150 bp paired end reads ...
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bioRxiv - Molecular Biology 2020Quote: ... Small RNA-seq libraries for 2–4 biological samples were sequenced together using a NextSeq 500 (Illumina) to obtain 75 nt ...
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bioRxiv - Genomics 2021Quote: ... Nuclear isolation of 5×10^4 cells was followed by treatment with Nextera Tn5 enzyme (Illumina, 20034198) for 45 minutes at 37°C ...
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bioRxiv - Immunology 2023Quote: ... We observed less than 4% barcode swapping between the two barcodes (more than 96% of the Illumina sequencing reads contained correct endodomain and barcode pairs) ...
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bioRxiv - Microbiology 2020Quote: ... MDA-amplified PCR products were then diluted to DNA concentrations of 0.2 ng μL−1 and used in metagenomic library preparations with the Nextera XT DNA Library Prep Kit (Illumina, San Diego), then quantified on an Agilent 2100 Bioanalyzer System (Agilent Genomics ...
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bioRxiv - Molecular Biology 2020Quote: Libraries of eDNA and iDNA samples at 50 ng μL−1 were sequenced using a MiSeq PE300 benchtop sequencer (Illumina, USA). Library preparation was done with a TruSeq DNA PCR-Free kit (LT Lib PREP KT-ST B PhiX control v3) ...
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bioRxiv - Developmental Biology 2021Quote: ... Illumina TruSeq RNA libraries were prepared from 1 μg total RNA and sequenced to generate 50 base pair single-end reads using the the HiSeq 1500 (Illumina, USA) at the Children’s Mercy OMICs Research Core Lab (Kansas City ...
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bioRxiv - Neuroscience 2020Quote: ... RNA-seq libraries were prepared from 1 μg of total RNA using the TruSeq Stranded mRNA LT Sample Prep kit (Illumina, USA). Sequencing was performed on the Illumina NovaSeq 6000 (CNS-RNA-seq cohort subset ...
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Cis-regulatory divergence underpins the evolution of C3-C4 intermediate photosynthesis in MoricandiabioRxiv - Plant Biology 2021Quote: ... cDNA libraries were prepared using 1 μg of total RNA with the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, USA). The cDNA library was qualified on the Agilent Technologies 2100 Bioanalyzer to check the library quality and fragment size of the sample ...
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bioRxiv - Cell Biology 2021Quote: ... Sequencing libraries were constructed from 1 ng of pre-amplified cDNA using DNA library preparation kit (TruePrep DNA Library Prep Kit V2 for Illumina, Vazyme). Libraries were sequenced on a HiSeq-PE150 ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 µg of total RNA was used for library preparation using the TruSeq Stranded mRNA kit (Illumina, cat# RS-122-2103) after rRNA depletion using Ribo-Zero plus (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... 1 µg of gDNA per sample was analyzed using the TruSeq® DNA PCR-Free Low-Throughput Library Prep Kit (Illumina) according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2022Quote: ... and sequenced on an Illumina NovaSeq using single-end 1×100 reads with the Illumina NovaSeq SP reagent kit (Illumina, 20027464). A total of 122,190,150 raw sequence reads (mean of 4,213,453 per sample (n = 29 ...
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bioRxiv - Molecular Biology 2021Quote: RNA-seq libraries were prepared with 1 µg of total RNA for each sample using the Illumina TruSeq mRNA kit (Illumina, USA) as recommended by the manufacturer using the Illumina CD RNA indexes (Illumina ...
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bioRxiv - Systems Biology 2020Quote: ... Final loading concentration of 1.8 pM and 1% PhiX (v/v) was used with the NextSeq® 500/550 High Output Kit v2 (150 cycles) (Illumina). Samples were sequenced paired-end with 75 cycles and single index of 6 cycles.
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bioRxiv - Immunology 2021Quote: ... Samples were sequenced on an Illumina Novaseq 6000 S4 flowcell (67 bp read 1 and 140 bp read 2) (Illumina, USA) to a depth of approximately 100,000 reads per cell ...
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bioRxiv - Immunology 2020Quote: ... Pooled libraries of 1 nM were denatured and diluted as per the Standard Normalization Method for the NextSeq® System (Illumina). Final loading concentration of 1.7 pM and 1% (v/v ...
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bioRxiv - Immunology 2020Quote: ... Final loading concentration of 1.7 pM and 1% (v/v) PhiX was used with the NextSeq® 500/550 High Output Kit v2 (150 cycles) (Illumina Samples were sequenced paired-end with 75 cycles of each read and single index of 6 cycles.
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bioRxiv - Immunology 2021Quote: ... whose lengths were about 600 base pairs were sequenced using an Illumina Novaseq 6000 S4 flowcell (67 bp read 1 and 140 bp read 2) (Illumina, USA). Only read2 contained the sequence regarding the definition of T cell clones.
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bioRxiv - Molecular Biology 2022Quote: ... PolyA + fraction (1 ng for each sample) was used for whole-transcriptome library preparation using ScriptSeq v2 RNA-Seq kit (Illumina, USA). Libraries amplified in 14 PCR cycles were purified using Agencourt AMPure XP beads (Beckman-Coulter ...
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bioRxiv - Neuroscience 2022Quote: ... Single-end sequencing (i.e, 1×75bp) was performed in a single run on an Illumina NextSeq 500 (Illumina, San Diego, CA). Three independent experimental replicates were run for the Cebpg shRNA-mediated knockdown and shRNA control transgenic lines for a total of 6 libraries.
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Dysregulated expanded endocannabinoid system as therapeutic targets of amyotrophic lateral sclerosisbioRxiv - Neuroscience 2024Quote: ... A library was independently prepared with 1 µg of total RNA for each sample by an Illumina TruSeq Stranded mRNA Sample Prep Kit (Illumina, Inc.). The first step in the workflow involved purifying the poly-A-containing mRNA molecules using poly-T-attached magnetic beads ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA from Col-0 and hakai-1 (Mock and Hpa) three biological replicates (same samples used for Illumina RNA-seq) were pooled into a single sample ...
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bioRxiv - Microbiology 2024Quote: Libraries for Illumina short read sequencing were prepared from 1 ng of extracted DNA utilizing the Nextera XT DNA Library Prep Kit according to the manufacturer’s recommendations (Illumina Inc., USA). Sequencing was carried out in paired-end (2×300 base pairs ...
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bioRxiv - Cancer Biology 2023Quote: ... RNAseq libraries were prepared from total RNA with the QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen, 015.24) in combination with the UMI Second Strand Synthesis Module for QuantSeq FWD (Illumina, Read 1) (Lexogen ...
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bioRxiv - Genetics 2023Quote: ... was used to isolate total RNA and approximately 1 ug of RNA was processed with the TruSeq Stranded mRNA Library Prep Kit (Illumina 20020594) to prepare libraries for paired-end sequencing on Nova-seq 6000 ...
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bioRxiv - Developmental Biology 2023Quote: ... PolyA+ RNA-seq libraries were produced from 1 µg of RNA using the TruSeq® Stranded mRNA Library Prep (Illumina, 20020594) kit ...
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bioRxiv - Synthetic Biology 2024Quote: ... Barcoded RNA-seq libraries were prepared from 1 μg of total RNA using the TruSeq Stranded mRNA Library Prep kit according to the manufacturer’s instructions (Illumina Inc., USA). RNA-seq libraries were sequenced on an Illumina NovaSeq 6000 System according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... Amplicon sequencing libraries were prepared as previously described54,70,71 and sequenced with a 1 x 75 single end read on a NextSeq instrument (Illumina, San Diego CA). Individual gRNA reads were extracted from FASTQ files with PoolQ and analyzed for enrichment using MAGeCK72 ...
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bioRxiv - Genomics 2024Quote: Both read 1 and read 2 files from each spatial region were mapped to the mm10 reference genome (source: Illumina igenomes) using the bowtie2 package (v2.5.1 ...
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bioRxiv - Developmental Biology 2024Quote: ... at 1/8th reaction volumes (except for Cas13d control and bckdk mRNA KD samples where 1/4th of the reaction volume were used) paired with IDT for Illumina DNA/RNA UD Indexes Set A (Illumina, 20027213), and purified using the Ampure XP bead-based reagent (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... the pool of plasmids for each library (inputs) was amplified using primers bearing sequences from Illumina (Read 1 and Read 2) and complementary to PT1 and down to Moe (Figure 1C) ...
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bioRxiv - Genomics 2024Quote: We then pooled the original and targeted libraries at a 2:1 molar concentration and sequenced them together on a NovaSeq 6000 (Illumina, 20012850) with a S1 Reagent Kit v1.5 (200 cycles ...
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bioRxiv - Genomics 2024Quote: ... where n is the number of reads covering each site and p is the NGS error rate (that we fixed to 1% as observed for the Illumina platform).
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bioRxiv - Genetics 2024Quote: ... 1 µg of total RNA was used with the TruSeq RNA Sample Prep Kit v.2 (Illumina, San Diego, CA, USA). The cDNA libraries were quantified by quantitative Real-Time PCR (qPCR) ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were prepared from 1 ng amplicon DNA by using the Nextera XT library prep kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Total RNA (1 μg) from each sample was used for NEBNext PolyA selection and Ultra Directional RNA Library preparation (Illumina, E7490, E7420). Prepared libraries were sequenced using 2×150 bp paired-end read chemistry on an Illumina HiSeq 4000 platform.
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bioRxiv - Molecular Biology 2021Quote: ... 1 ug of treated RNA was then input into the Illumina TruSeq Small RNA Library Preparation Kit (Illumina; Cat #: RS-200-0012) with 11 cycles of PCR amplification ...
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bioRxiv - Genomics 2020Quote: ... Samples were prepared for sequencing using 1 ug of genomic DNA following the TruSeq PCR free kit protocol (Illumina, FC-121-3001). Resulting libraries were quality checked on an Agilent DNA 1000 bioanalyzer (Agilent Technologies ...
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bioRxiv - Plant Biology 2020Quote: 42 RNA samples from experiment 1 were used for mRNA-Seq library preparation using the TruSeq Stranded mRNA Library Prep Kit (Illumina, CA, USA). Libraries were multiplexed and single-end 100 nt RNA-Seq was performed on a Novaseq 6000 at the GIGA Center (University of Liege ...
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bioRxiv - Genomics 2022Quote: ... a library was prepared from 1 μg of genomic DNA and a TruSeq DNA PCR-free Sample Preparation Kit (Illumina, CA, USA). Paired-end (151 bp per read ...
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bioRxiv - Genomics 2022Quote: ... Libraries were prepared with 1 μg of total RNA for each sample using a TruSeq Stranded mRNA Sample Prep Kit (Illumina, CA, USA). Paired-end sequencing (151 bp ...
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bioRxiv - Microbiology 2022Quote: ... The V4 region of the 16S rRNA gene was amplified from 1 or 7μl DNA and sequenced with a MiSeq (Illumina, San Diego, CA) using the 500 cycle MiSeq Reagent Kit ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was diluted 1:15 and subsequently tagmented and amplified for 12 cycles using the Nextera XT DNA Library Preparation Kit (Illumina FC-131). Individual libraries were QC’d by Qubit and Agilent Bioanalyzer ...
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bioRxiv - Genetics 2020Quote: ... One sequencing experiment (Experiment 1) was performed according to the manufacturer’s directions for the TruSeq Small RNA Sample Preparation Kit (Illumina, RS-200-0012). The protocol was adapted to incorporate a 2S blocking DNA oligo for removal of prevalent Drosophila small ribosomal RNA from the sequencing library [67] ...
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bioRxiv - Genomics 2020Quote: ... The cDNA library was generated from 1 µg of total RNA using TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA, USA) following manufacturer’s protocols ...
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bioRxiv - Immunology 2020Quote: ... The Illumina RNA-seq cDNA library was prepared using 1 μg of RNA from each sample and TruSeq V2 kits (Illumina, CA, USA), with reduced RNA fragmentation time (3 minutes ...