Labshake search
Citations for Illumina :
551 - 600 of 669 citations for 1 1 Dimethylsila 11 Crown 4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Illumina sequencing libraries with 6-bp single indices were constructed from 1 μg total RNA using the TruSeq Stranded mRNA LT kit (Illumina, RS-122-2101). The resulting library was validated using the Standard Sensitivity NGS Fragment Analysis Kit (AATI ...
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bioRxiv - Immunology 2024Quote: ... paired-end libraries were constructed from 1 µg of the initial genomic material using the TruSeq DNA v2 Sample Prep Kit (Illumina, #FC-121-2001) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Sequencing libraries were prepared from 1 ng of each VSG PCR product using the Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1096) following the manufacturer’s protocol except for the final cleanup step ...
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bioRxiv - Plant Biology 2022Quote: Sequencing libraries were generated with 300 ng to 1 μg of RNA using the TruSeq Stranded mRNA Sample Preparation Kit (Illumina, San Diego, CA). The libraries were assessed for size and purity using the Agilent 2100 Bioanalyzer and quantitated by qPCR using a KAPA Biosystems Library Quantitation kit (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... Multiplex samples Pool (1.65 pM including PhiX 1%) was loaded in NextSeq 500/550 High Output v2 kit (75 cycles) cartridge (catalog #FC-404-1005; Illumina, San Diego, CA) and loaded on NextSeq 500 System machine (Illumina) ...
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bioRxiv - Genomics 2023Quote: Poly-A selected stranded mRNA libraries were constructed from 1 μg total RNA using the Illumina TruSeq Stranded mRNA Sample Prep Kits (Illumina, San Diego, CA) according to manufacturer’s instructions with the following exception ...
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bioRxiv - Genomics 2024Quote: ... Genomic sequencing library was prepared from 1 ng of purified DNA using the Illumina Nextera XT library preparation kit (Illumina #FC-131-1096), following the manufacturer’s instructions or with a reduced volume of reagents to 20% ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA solutions of approximately ≥5 ng μL−1 were subjected to the tagmentation reaction using the Nextera DNA Flex Library Prep Kit (Illumina, San Diego, CA) was used at 1/40 scale of the provided protocol ...
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bioRxiv - Genomics 2024Quote: Illumina short-read sequencing libraries were prepared with 1 μg of high-quality RNA and the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Individual purified libraries were pooled into two six-sample equimolar pools containing the indexes 3-8 (NEBNext multiplex oligos for Illumina, set 1, E7335) and deep-sequenced at BGI on a HiSeq4000 (paired-end 100-bp reads).
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bioRxiv - Plant Biology 2024Quote: RNA samples were submitted to the Cornell Institute for Biotechnology Genomics Center for 3’ mRNA-seq library construction using a QuantSeq FWD kit (Lexogen, Vienna, Austria) followed by 1×75 bp sequencing on an Illumina NextSeq500 (Illumina, Hayward, CA, USA). Sequencing statistics were calculated with v0.15.0 seqkit stats with parameters -j 10 -T(Shen et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... and 250 ng of genomic DNA were genotyped for each sample using the Neuro Global Diversity Array-8 v.1 LCG with Neuro Booster content (Illumina, 20031815 and 20042459) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... or purified chromosomes (2 x 106 pelleted at 10,000g for 10min at 4°C) were resuspended in 50 μl transposase mixture (25 μl Illumina TD buffer ...
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bioRxiv - Genomics 2021Quote: ... We collected nuclei by centrifuging at 500 g at 4°C and resuspended nuclei in 5 ul TD buffer with 2.5 ul Tn5 enzyme (Illumina Tagment DNA TDE1 Enzyme and Buffer Kits) ...
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bioRxiv - Genetics 2022Quote: ... Nuclei pellet was obtained by centrifugation at 500g for 5 minutes at 4 degrees and resuspended in 100μl ice-cold 1x TD buffer (20034198, Illumina). About 10K nuclei was used for transposition reaction at 37 degrees for 30 minutes in a thermomixer ...
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bioRxiv - Microbiology 2023Quote: ... Libraries were pooled into a 4 nM solution and sequenced on the Illumina MiSeq platform (Illumina; San Diego; CA) with a target sequencing yield of 500MB-1GB per virome.
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bioRxiv - Microbiology 2022Quote: ... Library pools were diluted to 4 nM and denatured into single strands using fresh 0.2 N NaOH as recommended by Illumina. The final library was loaded at a concentration of 8 pM ...
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bioRxiv - Neuroscience 2023Quote: ... 4 saline) were pooled at equimolar concentrations and run with 75 bp paired-end reads on the MiSeq (Illumina).
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bioRxiv - Immunology 2022Quote: ... Library pools were diluted to 4 nM and denatured into single strands using fresh 0.2 N NaOH as recommended by Illumina. The final library was loaded at a concentration of 8 pM ...
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bioRxiv - Cell Biology 2021Quote: Libraries were generated from 1 µg of genomic DNA and high-throughput sequencing was performed on a HiSeq 3000 instrument (Illumina, San Diego, United States). Library preparation and Illumina sequencing was carried out by the Next Generation Sequencing (NGS ...
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bioRxiv - Plant Biology 2021Quote: ... 10 ng of template DNA and index primer 1 (N7xx) and index primer 2 (N5xx) of Nextera XT Index Kit v2 Set A (Illumina, San Diego, CA, USA) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... of the 16S rRNA gene and the internal transcribed spacer (ITS) 1 rDNA variable region on the Illumina MiSeq platform (Illumina, San Diego, CA, USA), as detailed previously (Phoma et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA (1 μg) was then converted into cDNA libraries according to the Illumina TruSeq Stranded mRNA Sample Preparation Guide (Illumina, San Diego, CA, USA) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Pooled libraries were diluted to 1.8 pM for 1×86 bp single-end sequencing with 75-cycle High Output v2 Kit on the NextSeq 550 Sequencing System (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... Pooled RPFs were diluted to 100 ng/uL and 1 ug was used as input for rRNA depletion using RiboZero Gold (part of Illumina 20020598, San Diego, CA) per manufacturer protocol ...
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bioRxiv - Physiology 2020Quote: ... RNA-seq libraries were sequenced using paired-end reads (50-nt read 1 and 25-nt read 2) on a NextSeq 500 instrument (Illumina, San Diego, CA, USA). Obtained reads were mapped to the mouse GRCm38.p6 genome using STAR (version 2.7.3a) ...
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bioRxiv - Genomics 2021Quote: ... reporter cDNA was PCR amplified using a reporter specific forward primer and a reverse primer binding the anchor sequence of the oligo- dT primer (corresponding to the Illumina TruSeq Read 1 sequence):
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bioRxiv - Neuroscience 2021Quote: ... Libraries were pooled and loaded for clustering on 1 lane of a Single-read Illumina Flow cell (for an average of 50 million of reads per library) (Illumina, San Diego, CA, USA). Reads of 100 bases were generated using the TruSeq SBS chemistry on an Illumina HiSeq 4000 sequencer (Illumina ...
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bioRxiv - Genomics 2021Quote: ... Illumina Catalogue FC-404-2003) following the Illumina recommended denaturation and loading recommendations which included a 1% PhiX spike (PhiX Control v3 Illumina Catalogue FC-110-3001).
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bioRxiv - Genomics 2020Quote: ... We spiked in 1% PhiX control (PhiX Control v3) in a 12 pM library and sequenced on an Illumina MiSeq platform (Illumina, San Diego, CA, USA) using a MiSeq Nano Reagent Kit v2 (500 cycles).
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bioRxiv - Cancer Biology 2022Quote: ... Libraries were pooled and sequenced single-end (1×75) on an Illumina NextSeq 500 using the High output V2 kit (Illumina Inc., San Diego CA). The RNA-seq samples were sequenced at a sequencing depth of 11-16 million reads.
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bioRxiv - Cell Biology 2024Quote: ... 8 bases for index 1 and 8 bases for index 2) on NextSeq 500 using the NextSeq 500 High Output Kit 75-cycles (Illumina, Cat# FC-404-1005). Flow lanes were loaded at 1.8pM ...
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bioRxiv - Cell Biology 2024Quote: ... 8 bases for index 1 and 8 bases for index 2) on NextSeq 500 using the NextSeq 500 High Output Kit 75-cycles (Illumina, Cat# FC-404-1005). Flow lanes were loaded at 1.8pM ...
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bioRxiv - Cell Biology 2023Quote: ... Libraries were prepared from 1 µg of input DNA using a TruSeq DNA Sample Prep Kit according to the manufacturer’s instructions (Illumina, Inc., San Diego, CA, USA). The sheared DNA fragments underwent end-repair ...
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bioRxiv - Microbiology 2023Quote: ... a sequencing library was generated from 1 ng of the resulting SAG DNA with the NexteraXT library preparation kit (Illumina, San Diego, CA, USA) and the library was then sequenced on an Illumina MiSeq instrument (2×300 bp) ...
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bioRxiv - Bioengineering 2023Quote: ... Sequencing was performed in paired-end mode with HighOutput flow cell (read 1: 28 cycles and read 2: 90 cycles) and a NextSeq 550 sequencer (Illumina, San Diego, CA, USA) at the Transcriptome Core Facility of the Institute in Regenerative Medicine and Biotherapy ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries were pooled and sequenced single-end (1×75) on an Illumina NextSeq 500 using the High output V2 kit (Illumina Inc., San Diego CA) at a sequencing depth of 23-31 million reads ...
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bioRxiv - Genomics 2022Quote: ... The concentration of amplified cDNA was measured with Qubit and 1 ng was used as input for Nextera XT (Illumina, San Diego, CA, USA) library preparation using New P5 SMART PCR oligo and Nextera index oligos ...
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bioRxiv - Genomics 2022Quote: ... 2 μg of Rohu-1 genomic DNA was used with an Illumina TruSeq DNA PCR-free Library Prep Kit (Illumina, San Diego, CA, USA) to create an Illumina sequencing library ...
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bioRxiv - Genomics 2022Quote: ... Illumina Catalogue FC-404-2003) following the Illumina recommended denaturation and loading recommendations which included a 1 % PhiX spike in (PhiX Control v3 Illumina Catalogue FC-110-3001). Data was uploaded to Basespace (www.basespace.illumina.com ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared from purified RNA (∼1 μg) using Illumina TruSeq standard mRNA library preparation kit as per manufacturer protocol (Illumina Inc., San Diego, CA). Briefly ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg total RNA was used for preparation of cDNA libraries with the TruSeq Small RNA Library Preparation Kit (Illumina, San Diego, CA, USA). Libraries were sequenced as 50 bp paired-end reads on an Illumina Genome Analyzer ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA samples were then diluted to 0.2-0.3 ng per uL and 1 ng of cDNA was carried over into library preparation using the Nextera XT DNA Library Preparation Kit (Illumina, cat. # FC-131-1096) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... RNA-seq libraries were prepared from 1 μg of total RNA using the Illumina TruSeq RNA Library Prep Kit (Illumina, San Diego, CA, USA) and sequenced on an Illumina NovaSeq 6000 platform (2 × 150 bp ...
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bioRxiv - Microbiology 2024Quote: ... DNA paired-end libraries (2 × 250-bp) were constructed with 1 ng of the viral genome as input using the Nextera XT DNA kit (Illumina, Inc., San Diego, USA) and sequenced on the Illumina MiSeq for 39-hours ...
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bioRxiv - Genetics 2024Quote: ... which uses proximity extension assay technology coupled to a readout methodology based on next generation sequencing (Illumina NovaSeq 6000, NextSeq 550, and NextSeq 2000; all manufactured by Illumina; appendix 1 p 2), to quantify protein targets ...
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bioRxiv - Molecular Biology 2024Quote: ... Library preparation for sequencing experiments was performed for Figure 1 using the Illumina TruSeq ChIP-Seq Library Preparation kit (Illumina, San Diego, CA, USA) and for Figure 2 using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Cancer Biology 2021Quote: ... The captured libraries were sequenced to achieve a mean unique coverage of at least 1000x using Illumina v.4 chemistry and paired-end 100-base pair (bp) reads (HiSeq, Illumina).
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bioRxiv - Cancer Biology 2021Quote: ... The flow cell underwent cluster amplification and massively parallel sequencing by synthesis using Illumina v.4 chemistry and paired-end 100-bp reads (Illumina).
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bioRxiv - Immunology 2021Quote: Paired-end RNA-Sequencing was performed on two independent biological replicates of 10 or 12 pooled 4-dpf larval zebrafish per genotypic group (nlrc3l mutants versus their siblings) by Illumina HiSeq 2×100 basepairs ...