Labshake search
Citations for Illumina :
251 - 300 of 9161 citations for RT PCR kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: WGS libraies were generated from 1 μg of 50 ng/ul high molecular weight gDNA using the TruSeq PCR-free Library Prep Kit (Illumina), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... 1 ng of the resulting cDNA from 14 PCR cycles was “tagmented” and barcoded by using a Nextera XT DNA Sample Preparation Kit (Illumina). The final libraries were purified by AmPure XP magnetic beads (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... Metagenomic libraries were prepared using 700 ng of DNA and the TruSeq DNA PCR-Free Library Prep Kit (Illumina Inc.) following the manufacturer’s recommended protocol ...
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bioRxiv - Microbiology 2020Quote: ... One microgram of gDNA with a DNA integrity number (DIN) of <=6 was used for library preparation using the TruSeq PCR-free library preparation kit (Illumina) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The 9 genomic DNA pools for sequencing were prepared by mixing equimolar concentrations of DNA from which 9 libraries were prepared with an insert size of 350 bp using a TruSeq PCR-free sample prep kit (Illumina), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... In the second method purified PCR amplicons (100 ng) were used to prepare sequencing libraries by TruSeq Nano DNA LT Library Preparation Kit (Illumina) with the following modifications ...
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bioRxiv - Cell Biology 2021Quote: ... The generated cDNA was 3′ end-adenylated and ligated to Illumina Paired-end sequencing adapters and amplified by PCR using HiSeq SR Cluster Kit v4 cBot (Illumina). Libraries were analyzed on a 2100 Bioanalyzer (Agilent ...
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bioRxiv - Genomics 2022Quote: ... A library was prepared using a TruSeq DNA PCR-Free library preparation kit according to manufacturer guidance and was sequenced on a NextSeq500 instrument (Illumina) with a NextSeq High Output v2 300 cycle kit ...
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bioRxiv - Neuroscience 2022Quote: ... and finally converted into DNA libraries using custom rpi primers (RNA PCR Primer Index) adapted from the Truseq Small RNA kit (Illumina)106 ...
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bioRxiv - Genomics 2022Quote: ... A paired-end library was prepared with the TruSeq DNA PCR-Free LT Sample Preparation Kit (Illumina, #FC-121-3001) from 1 µg of the genomic DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... Illumina sequencing libraries were prepared from the PCR-amplified cDNA from mutant lungs and controls using the Nextra DNA library prep kit (Illumina). Libraries were sequenced on a NovaSeq 6000 S Prime flowcell (Illumina ...
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bioRxiv - Microbiology 2020Quote: Sequencing libraries were prepared for each product of long PCR (ranging from 15 to 33 kb) using the Nextera XT DNA Sample Preparation Kit (Illumina) and sequenced on the Illumina MiSeq platform to generate paired-end (PE ...
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bioRxiv - Genomics 2019Quote: ... and then subjected to library construction using a TruSeq DNA PCR-Free HT sample prep kit (Illumina; San Diego, CA). The libraries were sequenced on a HiSeq 2500 system (Illumina ...
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bioRxiv - Microbiology 2021Quote: The second stage PCR procedure attached unique index primers to each sample using the Nextera XT v2 set A kit (Illumina). Purified DNA (5 μl ...
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bioRxiv - Microbiology 2021Quote: ... DNA libraries preparation with 16 cycles of PCR amplification and sequencing were done following manufacturer instructions (TruSeq ChIP-Library kit, NextSeq 500/550, Illumina).
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were prepared from 100 ng of DNA with the TruSeq DNA PCR-Free library Prep Kit from Illumina and sequenced on Illumina MiSeq platform with 150-bp paired-end read lengths (Institut Pasteur ...
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bioRxiv - Neuroscience 2020Quote: ... then the second-stage PCR was performed to attach Illumina i7 and i5 indexes (Illumina Nextera XT Library Preparation Kit) to individual samples ...
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bioRxiv - Cancer Biology 2022Quote: ... performed the library preparation for WGS of parental and resistant cells of cell lines #3 and #9 as well as a control tail samples corresponding to line #3 with the TruSeq DNA PCR-free Kit (Illumina) and the 150 bp paired-end sequencing on a HiSeq X (Illumina).
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bioRxiv - Microbiology 2020Quote: ... Sequencing libraries were prepared from 100 ng of DNA with the TruSeq DNA PCR-Free library Prep Kit from Illumina and sequenced on Illumina MiSeq platform with 150-bp paired-end read lengths (Institut Pasteur ...
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bioRxiv - Developmental Biology 2020Quote: ... A paired-end library with an average insert size of 450 bp was constructed with a TruSeq DNA PCR-free Library Prep Kit (Illumina) and two different mate-pair libraries (3 kb and 5 kb ...
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bioRxiv - Evolutionary Biology 2019Quote: ... A paired-end 150 bp library was prepared for each sample using the TruSeq DNA PCR-Free Library Prep Kit (Illumina), and sequenced on the Illumina HiSeq X platform ...
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bioRxiv - Molecular Biology 2021Quote: ... The generated cDNA was 3′ end-adenylated and ligated to Illumina Paired-end sequencing adapters and amplified by PCR using HiSeq SR Cluster Kit v4 cBot (Illumina). Libraries were analyzed on a 2100 Bioanalyzer (Agilent ...
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bioRxiv - Molecular Biology 2020Quote: ... Pools were sent to the University of Geneva for sequencing Illumina (MiSeq) where sequencing libraries were prepared using the reagents of the PCR-free TruSeq kit (Illumina).
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bioRxiv - Molecular Biology 2020Quote: ... The generated cDNA was 3′ end-adenylated and ligated to Illumina Paired-end sequencing adapters and amplified by PCR using HiSeq SR Cluster Kit v4 cBot (Illumina). Libraries were analyzed on a 2100 Bioanalyzer (Agilent ...
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bioRxiv - Immunology 2019Quote: ... adapter ligation and PCR amplification were performed using the TruSeq stranded total RNA with ribo-zero globin sample preparation kit (Illumina). The sequencing of transcriptome libraries was performed on the Illumina HiSeq 1500 platform (Illumina ...
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bioRxiv - Microbiology 2019Quote: ... Library preparation was carried out by the IBEST Genomic Resources Center at the University of Idaho (Moscow, ID): genomic DNA was used to make shotgun libraries using the TruSeq PCR-free Library Prep Kit (Illumina) with the HiSeq-length insert option (short) ...
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bioRxiv - Genetics 2021Quote: ... 700 ng of genomic DNA was sheared to an average size of 400 bp using a Covaris LE220 and was used as input to generate a whole-genome library using the TruSeq PCR-free kit (Illumina). The resulting DNA libraries were sequenced on Illumina Hi-seq X instrument to generate 2×150bp paired-end reads.
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bioRxiv - Genomics 2021Quote: ... Bisulfite-treated DNA samples (50 ng) were subjected to 10 cycles of PCR using random primers from the TruSeq DNA Methylation Kit (Illumina) to add adapters ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and subjected to a second PCR to introduce dual index sequences using Nextera XT Index Kits (Illumina, San Diego, CA). In the second PCR ...
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bioRxiv - Genomics 2021Quote: ... of Korea) for library preparation with 350 bp targeted insert size using TruSeq DNA PCR Free preparation kit (Illumina, USA) and sequencing on HiSeq X device (Illumina ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Subsequent to second round PCR amplification MiSeq sequencing libraries were generated using Nextera XT DNA Sample Preparation Kits (Illumina, Inc.). Amplicons for each sample were tagged using Nextera XT Index Kits (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... Purified PCR products were combined in equimolar concentrations and sequenced on an Illumina MiSeq (Miseq Reagent Kit V2, 50-cycle; Illumina) using primer PLM49 ...
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bioRxiv - Microbiology 2019Quote: ... we performed a second PCR step to add unique dual-index combinations to each sample using the Nextera XT index kit (Illumina). Second-step PCR amplifications were performed in a total volume of 25 μl using 2.5 μl of the PCR products ...
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bioRxiv - Immunology 2020Quote: ... First strand cDNA was subjected to limited PCR amplification followed by Tn5 transposon-based fragmentation using the Nextera XT DNA Library Preparation Kit (Illumina). Samples were then PCR amplified for 12 cycles using barcoded primers such that each sample carries a specific combination of eight base Illumina P5 and P7 barcodes for subsequent pooling and sequencing ...
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bioRxiv - Immunology 2020Quote: ... the 2nd SGA env PCR products were cleaved into approximately 300 bp products by tagmentation using the Nextera DNA Library Prep Kit (Illumina). Indices from the Nextera Index Kit (Illumina) ...
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bioRxiv - Microbiology 2021Quote: ... Libraries were prepared following the RNA-seq sample work flow using the Scriptseq Complete kit for bacteria and indexed with unique Scriptseq Index PCR primers (Illumina).
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bioRxiv - Genomics 2021Quote: ... Genomic DNA library was prepared and sequenced using a TruSeq DNA PCR-Free Library Prep Kit (Illumina, San Diego, CA).
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bioRxiv - Genomics 2021Quote: ... DNA was PCR amplified for a total of 11-13 cycles using barcoded primers (Illumina Nextera XT Index Kit v2) and purified using Ampure beads (1.4:1 beads:sample ratio) ...
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bioRxiv - Genomics 2021Quote: ... Direct tagmentation of the mRNA/cDNA hybrids and PCR amplification were performed on beads using Nextera XT DNA Library Prep Kit (Illumina); the Reference Guide was followed exactly for 20K and 10K samples ...
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bioRxiv - Genomics 2020Quote: ... The products were then amplified by 12 cycles of PCR using specific index adapters for Illumina sequencing (Nextera XT Index Kit v2, Illumina) (Supplementary Figure 1A) ...
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bioRxiv - Genetics 2020Quote: ... Two PCR-free libraries with insertion sizes of 350 bp and 550 bp were constructed for each cat using the TruSeq DNA PCR Free library preparation kit (Illumina). The Illumina HiSeq 2000 (Illumina ...
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bioRxiv - Genomics 2022Quote: Whole-genome shotgun libraries of 13 Capsicum lines were prepared with the TruSeq DNA PCR-Free Sample Prep Kit (Illumina), in accordance with the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Purified DNA was further amplified by PCR using Kapa Hifi Hotstart Ready Mix with Nextra XT Index Primers from Nextra XT Index Kit (Illumina). The PCR condition was ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were indexed using the Nextera XT Index Kit following the manufacturer’s instructions (Illumina, San Diego, CA, USA) and the concentration of the differently indexed samples determined using a KAPA Library Quantification Complete kit (Kapa Biosystems ...
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bioRxiv - Genomics 2022Quote: ... Sequencing libraries were prepared on 1µg of DNA using the TruSeq DNA PCR-Free Library Preparation Kit (Illumina, cat#20015963), with one additional bead cleanup performed after end-repair and after adapter ligation ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5 ng of PCR1 product was subjected to another round of PCR to add unique Illumina indexes (Nextera XT Index Kit, Illumina) with KAPA Hifi Hotstart Ready Mix (Roche ...
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bioRxiv - Immunology 2023Quote: ... Samples were then indexed by ligating index barcode to Illumina adapters onto the PCR amplicon using the Nextera XT Index Kit v2 Set A (Illumina). Indexed amplicon DNA concentrations in each sample were quantified ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared and indexed by adapter ligation and PCR (8 cycles) using the TruSeq Nano DNA Library Prep Kit (Illumina) and TruSeq DNA Single Indexes Set A or B (Illumina) ...
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bioRxiv - Molecular Biology 2023Quote: ... an individually tagged library was prepared from 125 ng of input material using the TruSeq DNA PCR-Free kit (Illumina). Since pools of amplicons do not need to be fragmented ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... we utilized an Illumina DNA Prep kit with Nextera DNA CD Indexes to create an NGS library from the purified PCR products (Illumina). The libraries were then pooled and transferred onto a MiniSeq sequencer (Illumina).