Labshake search
Citations for Illumina :
201 - 250 of 9161 citations for RT PCR kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Bead-bound DNA was amplified with 6 PCR amplification cycles using PE PCR 1.0 and PE PCR 2.0 primers (Illumina). Primary samples T-ALL 2-5 ...
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bioRxiv - Genetics 2023Quote: ... After a post-capture PCR (four amplification cycles using Illumina’s PE PCR 1.0 and PE PCR 2.0 primers), the PCHi-C libraries were purified with AMPure XP beads (Beckman Coulter #A63881 ...
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bioRxiv - Plant Biology 2020Quote: The genotypes were generated using an Eco RT (Illumina, San Diego, CA), a CFX 96 (Biorad ...
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Biosynthesis of Circular RNA ciRS-7/CDR1as Is Mediated by Mammalian-Wide Interspersed Repeats (MIRs)bioRxiv - Molecular Biology 2019Quote: ... a real-time PCR instrument (Eco Real-Time PCR System, Illumina) was used with the same primers (Table S1 ...
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bioRxiv - Cell Biology 2019Quote: ... The sequencing libraries were quantified by quantitative PCR (KAPA Biosystems Library Quantification Kit for Illumina platforms P/N KK4824) and Qubit 3.0 with dsDNA HS Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2021Quote: Purified samples were then amplified by PCR (maximum 12 cycles) using barcoded primers from the Nextera Index Kit (Illumina). To determine the optimal number of cycles for each sample ...
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bioRxiv - Genomics 2020Quote: Short-read sequence libraries for JI4 and JI128 were prepared using the TruSeq DNA PCR-Free Library Kit (Illumina), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... Library preparation was performed following the Illumina Truseq DNA PCR-Free LT Library Prep Kit (Illumina, San Diego, CA) protocol using 1ug of isolated genomic DNA ...
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bioRxiv - Genomics 2019Quote: ... The PCR products were then tagged using Nextera XT DNA Library Preparation Kit (Cat. 15032354, Illumina, San Diego, CA) and indexed using Nextera XT Index Kit (Cat ...
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bioRxiv - Microbiology 2019Quote: ... Libraries were prepared for sequencing on the HiSeq2500 platform with the TruSeq DNA PCR-free Library Prep Kit (Illumina). In total 83 P ...
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bioRxiv - Genomics 2020Quote: ... Libraries were generated from1 µg of genomic DNA using the TruSeq® DNA PCR-free Library Prep Kit (Illumina) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... PCR products were verified on 1.2% agarose gels and then indexed in a 2nd round PCR reaction using the Nextera Index kit (Illumina). PCR2 conditions for indexing were ...
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bioRxiv - Immunology 2022Quote: ... PCR products were purified and paired-end sequenced using the MiSeq v3 reagent kit (Illumina, San Diego, CA, USA). Reads with quality score of ≥ 30 and mean quality score ≥ 35 were further processed ...
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bioRxiv - Microbiology 2022Quote: ... Sequencing libraries were generated using the TruSeq DNA PCR-Free library prep kit (Illumina Inc., San Diego, CA, USA) and sequenced on an Illumina MiSeq instrument at 2 x 151 paired-end reads ...
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bioRxiv - Microbiology 2022Quote: ... Next generation libraries were generated using the TruSeq DNA PCR Free Nano kit (Illumina, Inc., San Diego, CA, USA) and the ARTIC multiplex PCR genome amplification protocol with the V3 primer scheme (www.protocols.io/view/ncov-2019-sequencing-protocol-bbmuik6w ...
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bioRxiv - Genomics 2022Quote: ... Libraries of 550 bp inserts were constructed using Truseq DNA PCR-Free kit and sequenced on NextSeq500 (Illumina, USA). Raw reads were filtered as described above ...
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bioRxiv - Microbiology 2022Quote: ... The H37Rv samples were library prepped using an Illumina DNA PCR-free library prep kit (Illumina, San Diego, USA) (Supplementary table 1) ...
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bioRxiv - Genomics 2021Quote: ... to prepare PCR-free paired-end libraries using the Illumina Genomic DNA Sample Preparation kit following the manufacturer’s instructions (Illumina). All paired-end libraries were sequenced on an Illumina NovaSeq 6000 system ...
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bioRxiv - Genomics 2020Quote: ... 600 pg of PCR product was prepared into Illumina sequencing libraries through tagmentation with a Nextera XT kit (Illumina). Tagmentation was performed according to manufacturer’s instructions and the library was amplified with primers Truseq5 and N700 series barcoded index primers ...
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bioRxiv - Genomics 2021Quote: ... The purified PCR products were then subjected to a multiplexing process using Nextera XT Index kit (Illumina, California, USA) in 50 μL reactions ...
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bioRxiv - Genomics 2019Quote: ... 415 out of 427 libraries were prepared the TruSeq DNA PCR-free Library Prep Kit (Illumina, San Diego, CA). For the remaining 12 libraries ...
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bioRxiv - Genomics 2022Quote: A short-read sequence library of ‘Takanotsume’ was prepared using the TruSeq DNA PCR-Free Sample Preparation Kit (Illumina) and sequenced on the NextSeq500 instrument (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... and libraries were quantitated with qPCR (Bio-rad CFX96 Touch Real-Time PCR, NEB Library Quant Kit for Illumina). Libraries were normalized to 0.5 nM and pooled ...
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bioRxiv - Bioengineering 2022Quote: ... Pooled PCR products were sequenced on the MiSeq using v2 2×250 base pair kit (Illumina, San Diego, CA). Sequences were processed in Mothur v ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were generated using the TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina, San Diego, CA, USA) following the manufacturer’s recommendations and index codes were added ...
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bioRxiv - Cancer Biology 2023Quote: ... indexed libraries for human WGS were constructed from 1.0 μg of gDNA using the TruSeq DNA PCR-Free Library Prep Kit (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and libraries were prepared using the Illumina Truseq DNA PCR-Free Sample preparation kit (Illumina Inc., San Diego, CA). Six pools were combined into a Miseq run (PE250 ...
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bioRxiv - Synthetic Biology 2023Quote: ... sequencing libraries were prepared using a TruSeq DNA PCR-free Sample Preparation Kit (Illumina, Inc., San Diego, CA, USA). The purified libraries were subjected to paired-end (2 × 150 bp ...
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bioRxiv - Microbiology 2023Quote: ... DNA-Seq libraries from 2.5 µg of genome DNA were prepared using the TruSeq DNA PCR-free kit (Illumina). Then ...
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bioRxiv - Microbiology 2023Quote: ... The sequencing library was constructed using the TruSeq DNA PCR-Free Library Prep Kit (Illumina, San Diego, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... This PCR product was prepared for sequencing using the Nextera XT DNA Sample Prep Kit (Illumina, FC-131-1096) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... cDNA libraries were amplified 12 cycles of PCR with Terra PCR Direct polymerase Mix in the presence of i5 or i7 PCR primers (Illumina) to add sample specific indexes ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was performed on an Eco Real-Time PCR system (Illumina) in triplicates using PerfeCTa SYBR Green FastMix (Quanta) ...
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bioRxiv - Genetics 2023Quote: ... After adapter ligation (Illumina PE PCR 1.0 and PE PCR 2.0 primers), the bead-bound Hi-C DNA was amplified with seven PCR amplification cycles ...
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bioRxiv - Plant Biology 2020Quote: ... to an average size of 500-bp and indexing with sequencing adapters using the TruSeq DNA (PCR free) kit (Illumina). Sequencing was performed on a single lane of an Illumina HiSeq2500 with 500 cycles in high-output mode ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR amplicons were purified by gel extraction and the concentrations of each sample was adjusted to 25 ng/µl for the 2nd stage barcoding PCR using TG Nextera XT Index Kit v2 Set A (Illumina) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... RNA-seq libraries were prepared from 500ng of RNA samples using an oligo dT based method with 15 cycles of PCR amplification with the Illumina TruSeq mRNA stranded kit (Illumina) and sequenced on the Illumina HiSeq 2000 v4 (50-bp single end read) ...
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bioRxiv - Developmental Biology 2021Quote: ... Input material ranged from 500pg to 15ng of immunoprecipitated DNA and processed according to the kit guidelines (NEBNext End Prep, Adaptor Ligation, PCR enrichment of Adaptor-Ligated DNA using NEBNext Multiplex Oligos for Illumina). Clean up and size selection were performed with AMPure beads (NEB) ...
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bioRxiv - Developmental Biology 2022Quote: ... The barcode sequencing libraries were quantified by quantitative PCR (KAPA Biosystems Library Quantification Kit for Illumina platforms P/N KK4824). Sequencing libraries were loaded at 18 pM on an Illumina HiSeq2500 using the following read length ...
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bioRxiv - Genomics 2020Quote: ... Successful amplicons were screened on a 1.5% agarose gel and further purified using Agencourt AMPure XP PCR Purification kit before proceeding to Nextera XT DNA Library Preparation (Illumina), according to manufacture instructions ...
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bioRxiv - Genomics 2020Quote: ... DNA samples were processed using the Illumina TruSeq DNA PCR-Free Sample Preparation kit (Illumina Inc., San Diego, CA, USA) on the Hamilton Microlab Star (Hamilton Robotics ...
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bioRxiv - Genomics 2020Quote: ... Paired-end libraries were prepared by the Purdue Genomics Core Facility using the Truseq DNA PCR-Free Sample Preparation Kit (Illumina). Whole-genome sequencing was performed using Illumina short-read technology (2×100bp ...
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bioRxiv - Immunology 2019Quote: ... and by enzymatic fragmentation of 300 pg of cDNA followed by 12 PCR cycles using the Nextera XT DNA Library Preparation Kit (cat# FC-131-1096, Illumina). Sequencing of the 15 mRNAseq libraries multiplexed together was carried out with an Illumina HiSeq2500 on a 2x125 bp paired-end run.
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bioRxiv - Immunology 2019Quote: ... and a mixture of 8 VH family and IgM-specific primers (listed below) containing 5’ adaptors to allow barcoding by the Illlumina TruSeq multiplex pcr kit (Illumina). Resulting PCR products were subjected to 10 cycles of PCR using Illumina TruSeq indexing primers ...
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bioRxiv - Genomics 2019Quote: ... and three mate-pair libraries (insert sizes of 2 kb, 5 kb, and 8 kb) were constructed using the TruSeq PCR-free Kit (Illumina) and Mate-pair Kit (Illumina) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we subjected the gel-purified product of the first PCR reaction to a second PCR reaction using a pair of index primers from Nextera XT DNA library preparation kit (Illumina). Fourth ...
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bioRxiv - Genomics 2019Quote: ... and ligated to TruSeq LT adapters using a TruSeq DNA PCR-Free Library Preparation Kit (Illumina, San Diego, CA, USA). We purified the ligation reaction using a Qiaquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Genomics 2021Quote: ... libraries with an insert size of 350 bp with the TruSeq DNA PCR-free gel-free library preparation kit (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... resequencing of genomic DNA was performed for accessions of ‘Kirakiraboshi,’ ‘Frau Yoshimi,’ ‘Posy Bouquet Grace,’ and ‘Blue Picotee Manaslu.’ Sequencing libraries (insert size of 500 bp) for the four lines were constructed with TruSeq DNA PCR-Free Library Prep Kit (Illumina) to sequence on a HiSeqX (Illumina) ...
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bioRxiv - Genetics 2019Quote: DNA sequencing libraries were constructed from 1 µg of genomic DNA using TruSeq DNA PCR-free Library Prep kit (Illumina). Sequencing was run on an Illumina HiSeq 2500 apparatus using a paired-end read length of 2×150 pb with the Illumina Reagent Kits as already described (Demars et al ...