Labshake search
Citations for Illumina :
151 - 200 of 9161 citations for RT PCR kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared using the TruSeq DNA PCR-Free High Throughput Library Prep Kit (Illumina) prior to sequencing ...
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bioRxiv - Cancer Biology 2023Quote: ... Sequencing libraries were generated using the TruSeq® DNA PCR- Free Sample Preparation Kit (Illumina, USA) following the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2023Quote: ... a paired-end sequencing library constructed using the TruSeq DNA PCR-Free sample preparation kit (Illumina) with an insert size of 550bp ...
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bioRxiv - Genomics 2023Quote: Illumina germline and tumor sequencing libraries were prepared using the TruSeq DNA PCR-Free kit (Illumina) for all samples ...
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bioRxiv - Plant Biology 2021Quote: ... A paired-end library and four mate libraries were constructed using a TruSeq DNA PCR-Free Library Prep Kit and a Nextera Mate Pair Sample Preparation Kit (Illumina, San Diego, CA), respectively ...
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bioRxiv - Immunology 2019Quote: ... before tagmentation and PCR amplification with index primers using the Nextera XT DNA SMP Prep Kit and Nextera XT IDX Kit (Illumina, San Diego, CA). After cleanup with Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... Library preparation is performed with or without amplification (Nextera® DNA Flex Library Prep kit or TruSeq® DNA PCR-Free kit, ILLUMINA®), either through automation (MICROLAB Star® ...
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bioRxiv - Genomics 2024Quote: ... and the final sequencing library was amplified utilizing the KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit along with index primers from the Nextera XT Index Kit (Illumina, FC-131-1001). The amplification protocol involved an initial step at 98°C for 45 seconds ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR products were then pooled and paired-end sequenced using the MiSeq Reagent Kit 2 from Illumina. Sequence reads were deposited to the SRA database with accession number ...
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bioRxiv - Cancer Biology 2020Quote: ... Subsequently sequencing libraries were prepared according to the TruSeq DNA PCR-Free Library Prep Kit (Illumina, USA). Resulting libraries were quantified with KAPA Library Quantification Kit for Illumina Platforms (Kapa Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... The barcoded sequencing libraries were quantified by quantitative PCR (KAPA Biosystems Library Quantification Kit for Illumina platforms). Sequencing libraries were loaded at 300 pM on an Illumina NovaSeq6000 using the following read length ...
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bioRxiv - Cell Biology 2021Quote: ... The purified DNA was PCR amplified for 5 cycles using a Nextera DNA Library Index kit (Illumina) and Phusion HF Master Mix (New England BioLabs ...
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bioRxiv - Microbiology 2020Quote: ... The second PCR was performed using the Nextera® XT Index Kit (Illumina, San Diego, CA, USA) for sample multiplexing with index adapters ...
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bioRxiv - Microbiology 2021Quote: Sequencing libraries were prepared from the env PCR products using the Nextera XT sample preparation kit (Illumina) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: Sequencing libraries were prepared from high-quality genomic DNA using the TruSeq DNA PCR-Free kit (Illumina) and ultra-deep whole genome sequencing (average 30X read-depth across the genome ...
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bioRxiv - Neuroscience 2022Quote: ... 600 pg of PCR product was used to generate Illumina sequencing library using Nextera XT kit (Illumina). The library was sequenced on a high output flow cell using Illumina NextSeq 500 ...
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bioRxiv - Molecular Biology 2019Quote: ... Each amplicon library was quantified by real time PCR using the KAPA Universal Library Quantification Kit (Illumina) per manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and DNA sequencing adaptor indexes ligated using the TruSeq DNA PCR-free LT Library Preparation Kit (Illumina). Quality control was performed on an Agilent 2100 BioAnalyser using high sensitivity DNA chip ...
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bioRxiv - Genetics 2021Quote: ... Sequencing libraries were prepared from high-quality genomic DNA using the TruSeq DNA PCR-Free kit (Illumina) and ultra-deep whole genome sequencing (average 30X read-depth across the genome ...
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bioRxiv - Genetics 2019Quote: Paired-end genomic libraries were constructed using TruSeq DNA PCR-free sample preparation kit (Illumina, CA, USA), and Illumina HiSeq2500 (Illumina v3 chemistry ...
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bioRxiv - Genomics 2020Quote: ... PCR amplification and pooled gel purification using Truseq Small RNA Library Prep Kit (Illumina, San Diego, USA). A flow cell containing millions of unique clusters was added to Illumina Hiseq2000 sequencer ...
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bioRxiv - Genomics 2022Quote: Pooled PCR products were subjected to multiplex NGS using the NextSeq 500/550 v2 Reagent Kit (Illumina). Sequenced reads were separated by barcode ...
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bioRxiv - Microbiology 2023Quote: ... and then libraries were prepared using the TruSeq DNA PCR Free High Throughput Library Prep Kit (Illumina). Paired-end reads of 100 bp were generated with an Illumina platform (library preparation and sequencing were performed by Macrogen Japan).
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bioRxiv - Molecular Biology 2023Quote: ... adapter ligation and PCR amplification were performed using the TruSeq Stranded total RNA sample preparation kit (Illumina). DNAs were sequenced using an Illumina HiSeq 4000 sequencer in 50 bp Single-Read following Illumina’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... the library was prepared using the TruSeq DNA PCR-Free Kit (Illumina, San Diego, CA, United States) and sequenced using Illumina NovaSeq 6000 ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared from the purified PCR product using the Nextera XT DNA Library kit (Illumina). After library preparation ...
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bioRxiv - Immunology 2023Quote: ... A library was constructed per individual tissues with the TruSeq DNA PCR-Free Library Prep Kit (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: Library preparation for all 40 samples was done using TruSeq PCR-free kits (Illumina, San Diego, USA) at the Institut Botànic de Barcelona (IBB – CSIC ...
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bioRxiv - Microbiology 2023Quote: ... and resulting PCR products were further purified and prepared for sequencing with the Nextera XT kit (Illumina). The final sequencing libraries were cleaned up with Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2024Quote: ... Barcodes were then introduced with a second round PCR using a legacy Nextera kit of adaptors (Illumina, compatible with FC-121-1031 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and libraries were constructed using Nextera Flex DNA pre-enrichment kit with 12 cycles of PCR amplification (Illumina). We re-sequenced samples from these original libraries to increase their depth if they had a RT-qPCR Ct values less than 20 from an RT-qPCR as measured in a previous paper [14] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Sequencing libraries for the new samples were prepared with a TruSeq DNA PCR-Free kit (Illumina, CA, USA) with a targeted insert size of 670 bp or with a Truseq DNA nano (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... Eluted DNA was amplified by PCR using NGS primers supplied with the kit (NEBNext Multiplex Oligos for Illumina, Index Primers Set 1 and 2 ...
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bioRxiv - Microbiology 2020Quote: ... The pair-end sequencing library was prepared using the TruSeq DNA PCR-Free Prep Kit (Illumina, United States). The prepared library was sequenced on the NovaSeq 6000 platform (2 × 150 bp chemistry ...
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bioRxiv - Microbiology 2022Quote: ... A second PCR step was performed with Illumina Nextera XT Index Kit v2 (Illumina, Cat.:FC-131-2001) and Ex Taq DNA Polymerase (TaKaRa Bio ...
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bioRxiv - Genomics 2022Quote: ... DNA libraries for whole-genome sequencing (WGS) were generated using Truseq DNA PCR-Free Library Prep Kits (Illumina). WGS was performed on either the Illumina HiSeq X Ten platform or the NovaSeq 6000 platform to generate mean coverage of 17.0× for 406 clonally expanded normal crypts ...
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bioRxiv - Bioengineering 2019Quote: ... Sequencing libraries were generated using TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina, San Diego, CA, USA) following the manufacturer’s recommendations and index codes were added ...
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bioRxiv - Genomics 2021Quote: Pooled PCR products were subjected to multiplex next-generation sequencing (NGS) using the MiSeq Reagent Kit v2 (Illumina) or the NextSeq 500/550 v2 Reagent Kit (Illumina) ...
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bioRxiv - Biochemistry 2021Quote: ... PCR products were gel purified in 3% agarose gel and qPCRed (using NEBNext Library Quant Kit for Illumina) to quantify concentration ...
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bioRxiv - Genetics 2021Quote: ... Multiplexed NGS libraries were prepared using the TruSeq PCR Free kit according to the manufacturer’s instructions (20015963, Illumina). The obtained libraries were sequenced using a Miseq 600 cycle kit to generate 150 bp and 300 bp paired-end reads to a depth of approximately 5-20X coverage ...
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bioRxiv - Genomics 2020Quote: ... followed with a 12.5 µL dual-indexing PCR amplification reaction (Nextera XT DNA Sample Preparation Index Kit, Illumina). In the unEQ ...
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bioRxiv - Plant Biology 2022Quote: ... One μg of genomic DNA was used for library construction with the Truseq DNA PCR-free kit (Illumina) following the manufacturer’s instructions for 350 bp insert size ...
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bioRxiv - Molecular Biology 2022Quote: ... and ligation of full-length barcoded Illumina adapters was performed using the TruSeq DNA PCR-free kit (Illumina) according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA libraries were prepared with TruSeq DNA PCR-Free sample preparation kit (Illumina Inc., San Diego, USA) and 2Ͱ×Ͱ150 paired-end sequencing was performed using the NextSeq 500/550 Illumina technology (Illumina ...
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bioRxiv - Genomics 2022Quote: All genomic DNA was subjected to random shotgun library preparation using the TruSeq DNA PCR-Free kit (Illumina). Ready-to-load libraries were sequenced on Illumina Miseq or HiSeq 3000 platforms (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: CUT & RUN libraries were prepared using the TruSeq ChIP-Sample Prep Kit-Set A and PCR Box (Illumina) from 5 ng of DNA with 10 PCR cycles for amplification following the manufacturer’s procedures ...
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bioRxiv - Immunology 2024Quote: ... The PCR products were then tagged using the Nextera XT Index Kit v2 Sets A to B (Illumina), with the amplicon concentrations normalized to 200 ng/µl via the Qubit high sensitivity dsDNA assay before pooling ...
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bioRxiv - Microbiology 2024Quote: ... A paired-end library was constructed using a TruSeq DNA PCR-Free Library Prep kit (Illumina, CA, USA) and size-selected on an agarose gel using a Zymoclean Large Fragment DNA Recovery Kit (Zymo Research ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were quantitated with qPCR (NEBNext Library Quant Kit for Illumina, Bio-rad CFX96 Touch Real-Time PCR). Libraries were normalized to 1.5 pM and pooled ...
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bioRxiv - Genomics 2019Quote: ... Bead-bound Hi-C DNA was amplified with 7 PCR amplification cycles using PE PCR 1.0 and PE PCR 2.0 primers (Illumina). After PCR amplification ...