Labshake search
Citations for Illumina :
1451 - 1472 of 1472 citations for ssc mir 17 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... variable regions V3 to V4 of the bacterial 16S ribosomal RNA gene were amplified by limited cycle PCR and subjected to the Illumina 16S metagenomic sequencing library preparation workflow according to the manufacturer’s recommendations (Illumina Inc., San Diego, CA, USA). The obtained DNA libraries were quantified ...
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bioRxiv - Cell Biology 2022Quote: ... as described in the section “Quantitative real-time PCR.” The library was prepared using the TruSeq stranded mRNA sample prep kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions and sequenced on an Illumina NovaSeq 6000 platform in the 101 bp single-end mode ...
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bioRxiv - Genomics 2022Quote: ... 2 μg of Rohu-1 genomic DNA was used with an Illumina TruSeq DNA PCR-free Library Prep Kit (Illumina, San Diego, CA, USA) to create an Illumina sequencing library ...
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bioRxiv - Plant Biology 2022Quote: Pair-end library preparation for MiSeq sequencing was conducted using the two-step tailed PCR method described on Illumina (Illumina, San Diego, CA, USA). We used the following primer pairs to amplify partial sequences of the 16S rRNA gene:
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bioRxiv - Microbiology 2022Quote: ... Purified PCR products were fragmented and tagged with the indexed adaptors using the Nextera DNA XT Sample Preparation Kit (Illumina, San Diego, CA, USA). The sequence library was quantified by using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... A second short-run PCR was performed in order to ligate the Illumina i7 and i5 barcodes (Nextera XT, Illumina, San Diego, CA, USA), and amplicons were purified again as above ...
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bioRxiv - Molecular Biology 2022Quote: ... A second PCR to insert Illumina adaptors (no further library preparation required) and amplicon sequencing was performed at Genewiz (Illumina-based Amplicon-EZ service).
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bioRxiv - Genomics 2023Quote: ... by the University of Wisconsin-Madison Biotechnology Center (Madison, WI, USA) as specified in the TruSeq DNA PCR-Free Reference Guide (Illumina, San Diego, CA, USA) [17] ...
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bioRxiv - Plant Biology 2023Quote: ... as per manufacturer’s instructions except that reaction volumes were scaled by one-half and 12 PCR cycles were used for library amplification (Illumina, San Diego, California, USA). Paired-end sequencing (2 × 150 bp ...
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bioRxiv - Microbiology 2024Quote: ... the V4 region within the 16S rRNA gene of bacterial DNA was amplified by PCR and sequenced using Illumina MiSeq platform (Illumina, San Diego, CA, USA). Raw sequences were processed following the Divisive amplicon denoising algorithm 2 (DADA2 ...
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bioRxiv - Genomics 2020Quote: ... After A-tailing and adaptor ligation fragments that carry adaptors on both ends were enriched by 8 cycles of PCR (Illumina TruSeq Nano DNA Sample Kit). The final library was quantified using PicoGreen (Quant-iT ...
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bioRxiv - Microbiology 2021Quote: ... Libraries for shotgun metagenomic sequencing were prepared using a PCR-free method at Novogene (Nanjing, China) on a HiSeq instrument (Illumina Inc. San Diego, CA, USA) with 150 bp paired-end reads and 6 Gb data/sample ...
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bioRxiv - Immunology 2020Quote: ... before fragmenting the samples to 450bp fragments and processing with the Illumina TruSeq DNA PCR-Free Sample Preparation kit (Illumina Inc., San Diego, CA, USA). Over the three-year duration of the sequencing phase of the project ...
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bioRxiv - Microbiology 2021Quote: ... and gDNA mocks were included in each PCR plate and sequenced alongside the samples sequenced with an Illumina MiSeq instrument (Illumina Inc., San Diego, CA, US) following manufacturer’s recommendations.
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bioRxiv - Microbiology 2022Quote: ... Selected samples from MPX virus-positive patients were directly sequenced without prior PCR amplification by the Illumina technology with the Nextera XT paired-end strategy on a MiSeq instrument (Illumina Inc., San Diego, CA, USA) or ...
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bioRxiv - Genetics 2024Quote: ... Samples were preamplified using High-Fidelity 2X PCR Master Mix (New England Bio-labs, #M0541L) and adapters (Illumina Nextera XT Index kit #FC-131-1001). The number of additional cycles was determined by quantitative PCR ...
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bioRxiv - Microbiology 2020Quote: ... A second PCR was performed to attach dual indices and Illumina® sequencing adapters using the Nextera XT Index kit (Illumina®; San Diego, CA, USA). The conditions of this PCR included ...
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bioRxiv - Microbiology 2023Quote: ... the purified PCR product was used to prepare 16S rRNA gene libraries which were then sequenced on an Illumina MiSeq instrument (Illumina Inc., San Diego, CA, United States) using the MiSeq reagent kit v3 (2 × 300 bp ...
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bioRxiv - Genomics 2024Quote: ... A pre-index anchor was ligated and a PCR amplification step with 15 cycles was done to add a 10bp unique dual index adapter sequences (IDT® for Illumina® RNA UD Indexes, Ligation). All libraries were quantified by Qubit dsDNA HS Assay measurement ...
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bioRxiv - Microbiology 2024Quote: Sequencing of genomic DNA of 21 evolved clones and the parental strain was performed on Illumina HiSeq X10 platform (Macrogen Korea, Geumcheon-gu, Seoul, Korea) with libraries prepared with Illumina-Shotgun library (Illumina TruSeq DNA PCR-free (350 bp insert)) ...
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bioRxiv - Genomics 2024Quote: ... bidentatus DNA extraction and library preparation was performed by Dovetail Genomics (Scotts Valley, CA, USA) with the TruSeq DNA PCR-Free kit (Trueq Index 5, Illumina, San Diego, CA, USA, mean size 475bp). 150 bp paired-end sequencing was performed on one lane of HiSeqX by Novogene (Cambridge ...
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bioRxiv - Cancer Biology 2020Quote: ... The PCR-amplified cDNA was purified using AMPure XP beads and processed with the Nextera XT DNA Library Preparation Kits (Illumina, Cat. # FC-131-1024 and FC-131-1096) as per the protocol provided by the manufacturer.