Labshake search
Citations for Illumina :
1051 - 1100 of 1399 citations for ssc mir 17 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... Sequencing libraries were prepared from high-quality genomic DNA using the TruSeq DNA PCR-Free kit (Illumina) and ultra-deep whole genome sequencing (average 30X read-depth across the genome ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products were cleaned and mixed with equal quantity and sequenced on Illumina MiSeq (Illumina, USA) at the Yale Center for Genome Analysis ...
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bioRxiv - Microbiology 2019Quote: ... 10 ng of DNA template was combined with PCR master mix (0.2 mM dNTP mix, 0.56 mg/ml BSA, 0.4 uM Illumina adapter sequence-tagged forward primer (515F ...
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bioRxiv - Genetics 2019Quote: Paired-end genomic libraries were constructed using TruSeq DNA PCR-free sample preparation kit (Illumina, CA, USA), and Illumina HiSeq2500 (Illumina v3 chemistry ...
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bioRxiv - Genomics 2020Quote: ... PCR amplification and pooled gel purification using Truseq Small RNA Library Prep Kit (Illumina, San Diego, USA). A flow cell containing millions of unique clusters was added to Illumina Hiseq2000 sequencer ...
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bioRxiv - Genomics 2022Quote: Pooled PCR products were subjected to multiplex NGS using the NextSeq 500/550 v2 Reagent Kit (Illumina). Sequenced reads were separated by barcode ...
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bioRxiv - Microbiology 2022Quote: ... Purified PCR products were pooled in equimolar and sequenced (PE300) on an Illumina MiSeq platform (Illumina, USA) following the standard protocols provided by Majorbio Bio-Pharm Technology Co ...
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bioRxiv - Plant Biology 2022Quote: ... was used for qPCR with an ECO Illumina Real-Time PCR system (Illumina, San Diego, CA; www.illumina.com). The presence of a single PCR product was confirmed by a melt curve ...
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bioRxiv - Immunology 2022Quote: ... PCR products were bar-coded (indexed) and sequenced using a HiSeq 2500 or NovaSeq6000 (both from Illumina). Sequenced reads (50 bp ...
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bioRxiv - Microbiology 2023Quote: ... and then libraries were prepared using the TruSeq DNA PCR Free High Throughput Library Prep Kit (Illumina). Paired-end reads of 100 bp were generated with an Illumina platform (library preparation and sequencing were performed by Macrogen Japan).
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bioRxiv - Molecular Biology 2023Quote: ... adapter ligation and PCR amplification were performed using the TruSeq Stranded total RNA sample preparation kit (Illumina). DNAs were sequenced using an Illumina HiSeq 4000 sequencer in 50 bp Single-Read following Illumina’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Index PCR was performed with 13 cycles using IDT for Illumina Nextera DNA Unique Dual Indexes (Illumina). Obtained libraries were purified with AMPure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... the library was prepared using the TruSeq DNA PCR-Free Kit (Illumina, San Diego, CA, United States) and sequenced using Illumina NovaSeq 6000 ...
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bioRxiv - Cell Biology 2023Quote: ... The obtained library is purified from the PCR products and sequenced using NextSeq 500 (Illumina, Inc., USA) to produce 75 base pair single-end reads ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... The 16S rDNA V3-V4 region was amplified by PCR and sequenced in the HiSeq platform (Illumina). The raw reads were processed and clustered into Operational Taxonomic Units (OTUs ...
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bioRxiv - Immunology 2023Quote: ... A second round of PCR was carried out with the IDT for Illumina UD indexes (Illumina Corp.) to add adapter tags ...
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bioRxiv - Genetics 2022Quote: ... PCR amplicons were subjected to 300 pair-end read sequencing using Illumina MiSeq (Illumina, San Diego, CA). The detailed methods are described in Supplemental methods.
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared from the purified PCR product using the Nextera XT DNA Library kit (Illumina). After library preparation ...
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bioRxiv - Molecular Biology 2023Quote: ... A 206 bp library fragment was generated using overlap PCR and included NexteraXT adapter overhang sequences (Illumina 16S Metagenomic Sequencing Library Preparation protocol ...
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bioRxiv - Immunology 2023Quote: ... A library was constructed per individual tissues with the TruSeq DNA PCR-Free Library Prep Kit (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: Library preparation for all 40 samples was done using TruSeq PCR-free kits (Illumina, San Diego, USA) at the Institut Botànic de Barcelona (IBB – CSIC ...
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bioRxiv - Microbiology 2024Quote: ... sequencing libraries were prepared from 1 µg of gDNA using the Illumina TruSeq DNA PCR free (Illumina) library preparation kit according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... and resulting PCR products were further purified and prepared for sequencing with the Nextera XT kit (Illumina). The final sequencing libraries were cleaned up with Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2024Quote: ... The mRNA levels of fepA and fes were quantified by qPCR (Illumina Eco Real-Time PCR System), using the SYBR Green Master mix (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... Barcodes were then introduced with a second round PCR using a legacy Nextera kit of adaptors (Illumina, compatible with FC-121-1031 ...
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bioRxiv - Biochemistry 2023Quote: ... Indexed PCR amplicons were pooled together in an equimolar ratio for paired-end sequencing by MiSeq (Illumina) with the 300-cycle MiSeq Reagent Nano Kit v2 (Illumina ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and libraries were constructed using Nextera Flex DNA pre-enrichment kit with 12 cycles of PCR amplification (Illumina). We re-sequenced samples from these original libraries to increase their depth if they had a RT-qPCR Ct values less than 20 from an RT-qPCR as measured in a previous paper [14] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Sequencing libraries for the new samples were prepared with a TruSeq DNA PCR-Free kit (Illumina, CA, USA) with a targeted insert size of 670 bp or with a Truseq DNA nano (Illumina ...
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bioRxiv - Synthetic Biology 2021Quote: ... The purified PCR products were then subjected to NGS-based amplicon sequencing (Illumina 100-bp paired end sequencing), which was performed at the Genome Sequencing Facility (GSF ...
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bioRxiv - Microbiology 2020Quote: ... The pair-end sequencing library was prepared using the TruSeq DNA PCR-Free Prep Kit (Illumina, United States). The prepared library was sequenced on the NovaSeq 6000 platform (2 × 150 bp chemistry ...
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bioRxiv - Microbiology 2022Quote: ... A second PCR step was performed with Illumina Nextera XT Index Kit v2 (Illumina, Cat.:FC-131-2001) and Ex Taq DNA Polymerase (TaKaRa Bio ...
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bioRxiv - Genomics 2022Quote: ... DNA libraries for whole-genome sequencing (WGS) were generated using Truseq DNA PCR-Free Library Prep Kits (Illumina). WGS was performed on either the Illumina HiSeq X Ten platform or the NovaSeq 6000 platform to generate mean coverage of 17.0× for 406 clonally expanded normal crypts ...
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bioRxiv - Bioengineering 2019Quote: ... Sequencing libraries were generated using TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina, San Diego, CA, USA) following the manufacturer’s recommendations and index codes were added ...
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bioRxiv - Genomics 2019Quote: ... equivalent to the protocol in the Illumina TruSeq PCR-Free Sample Preparation Guide (Illumina cat# FC-121-2001). Center-specific details are available from the TOPMed website82 ...
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Macrobdella decora: Old World Leech Gut Microbial Community Structure Conserved in a New World LeechbioRxiv - Microbiology 2019Quote: ... PCR reactions were prepared as in (58) and sequenced using an Ilumina MiSeq (Illumina San Diego, CA U.S.A.) with custom sequencing primers added to the reagent cartridge (73 ...
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bioRxiv - Genetics 2020Quote: ... Plates were immediately transferred to ice post-incubation and 12 μL of PCR mix (7.5 μL NPM [Illumina Inc ...
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bioRxiv - Genomics 2021Quote: Pooled PCR products were subjected to multiplex next-generation sequencing (NGS) using the MiSeq Reagent Kit v2 (Illumina) or the NextSeq 500/550 v2 Reagent Kit (Illumina) ...
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bioRxiv - Biochemistry 2021Quote: ... PCR products were gel purified in 3% agarose gel and qPCRed (using NEBNext Library Quant Kit for Illumina) to quantify concentration ...
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bioRxiv - Genetics 2021Quote: ... Multiplexed NGS libraries were prepared using the TruSeq PCR Free kit according to the manufacturer’s instructions (20015963, Illumina). The obtained libraries were sequenced using a Miseq 600 cycle kit to generate 150 bp and 300 bp paired-end reads to a depth of approximately 5-20X coverage ...
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bioRxiv - Genomics 2020Quote: ... followed with a 12.5 µL dual-indexing PCR amplification reaction (Nextera XT DNA Sample Preparation Index Kit, Illumina). In the unEQ ...
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bioRxiv - Plant Biology 2022Quote: ... One μg of genomic DNA was used for library construction with the Truseq DNA PCR-free kit (Illumina) following the manufacturer’s instructions for 350 bp insert size ...
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bioRxiv - Molecular Biology 2022Quote: ... and ligation of full-length barcoded Illumina adapters was performed using the TruSeq DNA PCR-free kit (Illumina) according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2022Quote: ... of the 16S rRNA gene was amplified by PCR (341F_primer: CCTACGGGNGGCWGCAG, 806R_primer: GACTACHVGGGTATCTAATCC) and sequenced on the MiSeq platform (Illumina) using 2 × 300 base pair paired-end protocol ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA libraries were prepared with TruSeq DNA PCR-Free sample preparation kit (Illumina Inc., San Diego, USA) and 2Ͱ×Ͱ150 paired-end sequencing was performed using the NextSeq 500/550 Illumina technology (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... Adaptor ligated cDNA was PCR enriched to incorporate an Illumina compatible index sequence (NEBNext Multiplex Oligos for Illumina, Dual Index Primers Set1 ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting PCR amplicons were purified using Ampure XP Beads and sequenced using the HiSeq 2500 system (Illumina) to assess changes in abundance of sgRNAs between Ogt fl and Ogt iKO groups ...
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bioRxiv - Genomics 2022Quote: All genomic DNA was subjected to random shotgun library preparation using the TruSeq DNA PCR-Free kit (Illumina). Ready-to-load libraries were sequenced on Illumina Miseq or HiSeq 3000 platforms (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... the final PCR products from multiple libraries were typically combined and sequenced on a NextSeq 500 system (Illumina) in 2×80 bp paired-end mode.
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bioRxiv - Systems Biology 2023Quote: ... Each appropriate sized PCR product was purified and then use the HiSeq 2500 genome analyzer (Illumina HiSeq 2500) to perform the 250-bp nucleotide paired-end sequencing ...
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bioRxiv - Genomics 2023Quote: ... 12.5 μl of a master mix containing 7.5 μl of Nextera PCR Master Mix (Illumina, FC-131-1096) and 2.5μl of each Index primer i7 (Illumina ...